1. A system comprising:
a pipettor;
a reagent pack comprising a well containing a reagent;
a reagent storage unit configured to hold the reagent pack, the reagent storage unit including:
a cavity containing the reagent pack;
a latch arranged about the cavity, the latch configured to secure and align the reagent pack within the cavity, the latch including a releasing feature; and
a cover disposed over the cavity and latch, the cover including a first aperture and a second aperture, wherein the first aperture aligns over the well of the reagent pack thereby providing the pipettor access to the reagent contained in the well, and the second aperture aligns over the releasing feature thereby providing the pipettor access to actuate the releasing feature to unsecure the reagent pack from the latch.
2. The system of claim 1, wherein the latch is a mechanical latch.
3. The system of claim 1, wherein the reagent pack further comprises a second well, wherein the cover further includes a third aperture, and the third aperture of the cover aligns over the second well thereby providing the pipettor access to the second well, wherein the first, the second, and the third apertures are arranged linearly.
4. The system of claim 1, wherein the pipettor comprises a pipette tip suitable to extend through the first aperture and aspirate the reagent contained in the well.
5. The system of claim 1, wherein the reagent storage unit is configured to hold a plurality of reagent packs in the cavity, the reagent storage unit includes a latch including a releasing feature release to secure each one of the plurality of reagent packs, the cover includes apertures to access each reagent pack and its corresponding releasing feature, wherein the reagent storage unit includes containment walls arranged between the reagent packs held in the cavity.
6. The system of claim 1 wherein the reagent storage unit further comprises a fan, the fan configured to remove heat from the reagent storage unit, wherein the speed of the fan is adjusted by a controller in response to ambient temperature.
7. The system of claim 1, where the reagent pack comprises a reagent that is specific for an assay for a specific analyte.
8. The system of claim 1, wherein the latch comprises a fastener that is configured to engage a mating feature of the reagent pack, and wherein the system further comprises a resilient member that is interposed between the reagent pack and the distal wall when the reagent pack is held in the reagent storage unit, such that the resilient member provides an impelling force that directs the reagent pack toward a proximal wall of the reagent storage unit on disengagement of the fastener from the mating feature.
9. The system of claim 5, wherein the resilient member is configured to act as an electrical ground.
10. A method comprising:
aligning a consumable pack in a storage unit, wherein the consumable pack comprises consumables that are manipulated using a pipettor;
securing the consumable pack within the storage unit by engaging a latch having a releasing feature with a mating feature of the consumable pack; and,
releasing the consumable pack by;
aligning the pipettor with the releasing feature;
moving the pipettor towards the releasing feature; and
contacting the releasing feature with the pipettor,
thereby causing the latch to disengage from the mating of the consumable pack.
11. The method of claim 10, wherein the pipettor comprises a pipette tip suitable to extend into the storage unit and contact the consumables of the consumable pack.
12. The method of claim 10, wherein the consumable pack is a pipette tip rack.
13. The method of claim 10, wherein the consumable pack is a reagent pack.
14. The method of claim 13, wherein
the pipettor transfers an isolated nucleic acid to a reaction vessel stored in an assay cartridge;
the pipettor transfers reagents contained in the reagent pack to the reaction vessel; and,
the pipettor transfers the reaction vessel to a thermal cycler.
15. The method of claim 13, wherein
the pipettor transfers reagents contained in the reagent pack to a reaction vessel stored in an assay cartridge;
the pipettor transfers an isolated nucleic acid to the reaction vessel; and,
the pipettor transfers the reaction vessel to a thermal cycler.
16. The method of claim 14 or 15, wherein the reaction vessel is located in a compartment of an assay cartridge, the assay cartridge comprising a reaction well containing an isolated nucleic acid.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A polyisocyanate derived adduct comprising
the reaction product of a urethane prepolymer and a capping agent,
the capping agent selected from the group consisting of a methoxypolyoxyethylene monols, polyoxyethylene-polyoxypropylene monols, and mixtures thereof, and
wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
2. The polyisocyanate derived adduct of claim 1 wherein the dihydroxy functional polyol is selected from the group consisting of polyoxyethylene glycol and polyoxyethylene polyoxypropylene glycols
3. The polyisocyanate derived adduct of claim 2 wherein the methoxypolyoxyethylene monols have a molecular weight of about 350 to about 750, and the polyoxyethylene-polyoxypropylene monols have a molecular weight of about 270 to about 3930.
4. The polyisocyanate derived adduct of claim 2 wherein the urethane prepolymer and the capping agent is selected from the group consisting of methoxypolyoxyethylene monols, and mixtures of methoxypolyoxyethylene monols with polyoxyethylene-polyoxypropylene monols.
5. The polyisocyanate derived adduct of claim 4 wherein the urethane prepolymer and the capping agent are present in a weight ratio of urethane prepolymer to capping agent of about 2:1 to about 1:2.
6. The polyisocyanate derived adduct of claim 4 wherein the dihydroxy functional polyol and the isocyanate are present in a weight ratio of dihydroxy functional polyol to isocyanate of about 2:1 to about 25:1.
7. The polyisocyanate derived adduct of claim 2 wherein the capping agent is polyoxyethylene-polyoxypropylene monol, and the capping agent and the urethane prepolymer are present in a weight ratio of the capping agent to the prepolymer of about 1:1 to about 1:3.
8. The polyisocyanate derived adduct of claim 1 wherein the dihydroxy functional polyol is polyoxyethylene glycol, the isocyanate is 4,4-diphenylmethane diisocyanate, and the capping agent is a methoxypolyoxyethylene monol.
9. The polyisocyanate derived adduct of claim 1 wherein the dihydroxy functional polyol is polyoxyethylene-polyoxypropylene glycol, the isocyanate is 4,4-diphenylmethane diisocyanate, and the capping agent is selected from the group consisting of methoxypolyoxyethylene monol, and mixtures of methoxypolyoxyethylene monol with polyoxyethylene-polyoxypropylene monol.
10. An emulsifiable polyisocyanate comprising an isocyanate and a polyisocyanate derived adduct comprising the reaction product of a urethane prepolymer and a capping agent,
the capping agent selected from the group consisting of a methoxypolyoxyethylene monols, polyoxyethylene-polyoxypropylene monols, and mixtures thereof, and
wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
11. The emulsifiable polyisocyanate of claim 10 wherein the dihydroxy functional polyol is selected from the group. consisting of polyoxyethylene glycol and polyoxyethylene polyoxypropylene glycols
12. The emulsifiable polyisocyanate of claim 11 wherein the methoxypolyoxyethylene monols have a molecular weight of about 350 to about 750, and the polyoxyethylene-polyoxypropylene monols have a molecular weight of about 270 to about 3930.
13. The emulsifiable polyisocyanate of claim 11 wherein the urethane prepolymer and the capping agent is selected from the group consisting of methoxypolyoxyethylene monols, and mixtures of methoxypolyoxyethylene monols with polyoxyethylene-polyoxypropylene monols.
14. The emulsifiable polyisocyanate of claim 13 wherein the urethane prepolymer and the capping agent are present in a weight ratio of urethane prepolymer to capping agent of about 2:1 to about 1:2.
15. The emulsifiable polyisocyanate of claim 13 wherein the dihydroxy functional polyol and the isocyanate are present in a weight ratio of dihydroxy functional polyol to isocyanate of about 2:1 to about 25:1.
16. The emulsifiable polyisocyanate of claim 11 wherein the capping agent is polyoxyethylene-polyoxypropylene monol, and the capping agent and the urethane prepolymer are present in a weight ratio of the capping agent to the prepolymer of about 1:1 to about 1:3.
17. The emulsifiable polyisocyanate of claim 10 wherein the dihydroxy functional polyol is polyoxyethylene glycol, the isocyanate is 4,4-diphenylmethane diisocyanate, and the capping agent is a methoxypolyoxyethylene monol.
18. The emulsifiable polyisocyanate of claim 10 wherein the dihydroxy functional polyol is polyoxyethylene-polyoxypropylene glycol, the isocyanate is 4,4-diphenylmethane diisocyanate, and the capping agent is selected from the group consisting of methoxypolyoxyethylene monol, and mixtures of methoxypolyoxyethylene monol with polyoxyethylene-polyoxypropylene monol.
19. An aqueous emulsion having extended potlife under conditions of high temperature and humidity,
the emulsion comprising water an emulsifiable polyisocyanate,
the emulsifiable polyisocyanate comprising an isocyanate and a polyisocyanate derived adduct that comprises
20. An aqueous emulsion comprising an emulsifiable polyisocyanate, the emulsifiable polyisocyanate comprising an isocyanate and a polyisocyanate derived adduct, the adduct comprising the reaction product of a urethane prepolymer and a capping agent,
the capping agent selected from the group consisting of a methoxypolyoxyethylene monols, polyoxyethylene-polyoxypropylene monols, and mixtures thereof, and
wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
21. The aqueous emulsion of claim 20 wherein the dihydroxy functional polyol is selected from the group consisting of polyoxyethylene glycol and polyoxyethylene polyoxypropylene glycols
22. The aqueous emulsion of claim 21 wherein the methoxypolyoxyethylene monols have a molecular weight of about 350 to about 750, and the polyoxyethylene-polyoxypropylene monols have a molecular weight of about 270 to about 3930.
23. The aqueous emulsion of claim 21 wherein the urethane prepolymer and the capping agent is selected from the group consisting of methoxypolyoxyethylene monols, and mixtures of methoxypolyoxyethylene monols with polyoxyethylene-polyoxypropylene monols.
24. The aqueous emulsion of claim 23 wherein the urethane prepolymer and the capping agent are present in a weight ratio of urethane prepolymer to capping agent of about 2:1 to about 1:2.
25. The aqueous emulsion of claim 23 wherein the dihydroxy functional polyol and the isocyanate are present in a weight ratio of dihydroxy functional polyol to isocyanate of about 2:1 to about 25:1.
26. The aqueous emulsion of claim 21 wherein the capping agent is polyoxyethylene-polyoxypropylene monol, and the capping agent and the urethane prepolymer are present in a weight ratio of the capping agent to the prepolymer of about 1:1 to about 1:3.
27. The aqueous emulsion of claim 20 wherein the dihydroxy functional polyol is polyoxyethylene glycol, the isocyanate is 4,4-diphenylmethane diisocyanate, and the capping agent is a methoxypolyoxyethylene monol.
28. The aqueous emulsion of claim 20 wherein the dihydroxy functional polyol is polyoxyethylene-polyoxypropylene glycol, the isocyanate is 4,4-diphenylmethane diisocyanate, and the capping agent is selected from the group consisting of methoxypolyoxyethylene monol, and mixtures of methoxypolyoxyethylene monol with polyoxyethylene-polyoxypropylene monol.
29. An emulsifiable polyisocyanate comprising the reaction product of polymeric methane diphenyl diisocyante, methoxypolyoxyethylene monol, and a polyisocyanate derived adduct,
the adduct comprising the reaction product of a urethane prepolymer and a methoxypolyoxyethylene monol,
wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
30. The emulsifiable polyisocyanate of claim 29 wherein the polymeric methane diphenyl diisocyante is present in an amount of about 92 wt. %, the methoxypolyoxyethylene monol is present in an amount of about 4 wt. %, and the polyisocyanate derived adduct is present in an amount of about 4 wt. %.
31. The emulsifiable polyisocyanate of claim 30 wherein the dihydroxy functional polyol is polyoxyethylene glycol.
32. The emulsifiable polyisocyanate of claim 31 wherein the methoxypolyoxyethylene monol has a molecular weight of about 350.
33. An emulsifiable polyisocyanate comprising the reaction product of uretomine modified 4,4-diphenylmethane diisocyanate having about 26% NCO, and
a mixture of methoxypolyoxyethylene monol and a polyisocyanate derived adduct comprising the reaction product of a urethane prepolymer and a methoxypolyoxyethylene monol, wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
34. The emulsifiable polyisocyanate of claim 33 wherein the modified diisocyante is present in an amount of about 90 wt. %, the methoxypolyoxyethylene monol is present in an amount of about 6 wt. %, and the polyisocyanate derived adduct is present in an amount of about 4 wt. %.
35. The emulsifiable polyisocyanate of claim 34 wherein the dihydroxy functional polyol is polyoxyethylene glycol.
36. The emulsifiable polyisocyanate of claim 35 wherein the methoxypolyoxyethylene monol has a molecular weight of about 350.
37. An emulsifiable polyisocyanate comprising the reaction product of uretonimine modified 4,4-diphenylmethane diisocyanate having a NCO value of about 29.3%, and a mixture of methoxypolyoxyethylene monol and a polyisocyanate derived adduct comprising the reaction product of a urethane prepolymer and a methoxypolyoxyethylene monol, wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
38. The emulsifiable polyisocyanate of claim 37 wherein the uretomine modified diisocyante is present in an amount of about 92 wt. %, the methoxypolyoxyethylene monol is present in an amount of about 4 wt. %, and the polyisocyanate derived adduct is present in an amount of about 4 wt. %.
39. The emulsifiable polyisocyanate of claim 38 wherein the dihydroxy functional polyol is polyoxyethylene glycol.
40. The emulsifiable polyisocyanate of claim 39 wherein the methoxypolyoxyethylene monol has a molecular weight of about 750.
41. An aqueous latex emulsion having improved potlife comprising an aqueous latex emulsion and an emulsifiable isocyanate,
the emulsifiable polyisocyanate selected from the group consisting of
a. a first reaction product of polymeric methane diphenyl diisocyante, methoxypolyoxyethylene monol, and a polyisocyanate derived adduct, the adduct comprising the reaction product of a urethane prepolymer and a methoxypolyoxyethylene monol, wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate,
b. a second reaction product of product of uretomine modified 4,4-diphenylmethane diisocyanate having about 26% NCO, and a mixture of methoxypolyoxyethylene monol and a polyisocyanate derived adduct comprising the reaction product of a urethane prepolymer and a methoxypolyoxyethylene monol, wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate,
c. a third reaction product of uretonimine modified 4,4-diphenylmethane diisocyanate having a NCO value of about 29.3%, and a mixture of methoxypolyoxyethylene monol and a polyisocyanate derived adduct comprising the reaction product of a urethane prepolymer and a methoxypolyoxyethylene monol, wherein the urethane prepolymer is the reaction product of a dihydroxy functional polyol and an isocyanate.
42. The emulsion of claim 41 wherein in the first reaction product the polymeric methane diphenyl diisocyante is present in an amount of about 92 wt. %, the methoxypolyoxyethylene monol is present in an amount of about 4 wt. %, and the polyisocyanate derived adduct is present in an amount of about 4 wt. %.
43. The emulsion of claim 42 wherein the dihydroxy functional polyol is polyoxyethylene glycol, and the methoxypolyoxyethylene monol has a molecular weight of about 350.
44. The emulsion of claim 41 wherein in the second reaction product the modified diisocyante is present in an amount of about 90 wt. %, the methoxypolyoxyethylene monol is present in an amount of about 6 wt. %, and the polyisocyanate derived adduct is present in an amount of about 4 wt. %.;
45. The emulsion of claim 44 wherein the dihydroxy functional polyol is polyoxyethylene glycol and the methoxypolyoxyethylene monol has a molecular weight of about 350.
46. The emulsion of claim 41 wherein in the third reaction product the uretomine modified diisocyante is present in an amount of about 92 wt. %, the methoxypolyoxyethylene monol is present in an amount of about 4 wt. %, and the polyisocyanate derived adduct is present in an amount of about 4 wt. %.
47. The emulsion of claim 46 wherein the dihydroxy functional polyol is polyoxyethylene glycol and the methoxypolyoxyethylene monol has a molecular weight of about 750.
48. An emulsifiable polyisocyanate comprising,
the reaction product of polymeric methane diphenyl diisocyante, a polyisocyanate derived adduct wherein the adduct is the reaction product of a urethane prepolymer formed by reacting a urethane prepolymer of polyoxyethylene-polyoxypropylene glycol and 4,4-diphenylmethane diisocyanate with a first methoxypolyoxyethylene monol having a molecular weight, a polyoxypropylene glycol and a second methoxypolyoxyethylene monol having a molecular weight differenct from the molecular weight of the first methoxypolyoxyethylene.
49. The emulsifiable isocyanate of claim 48 wherein the polymeric methane diphenyl diisocyante is present in an amount of about 93%, the polyisocyanate derived adduct is present in an amount of about 3%, the polyoxypropylene glycol is present in an amount of about 1.5%, and the second methoxypolyoxyethylene monol is present in an amount of about 2.5%.
50. The emulsifiable isocyanate of claim 49 wherein the polyoxypropylene glycol has a molecular weight of abut 725, and the second methoxypolyoxyethylene monol has a molecular weight of about 750.
51. An emulsion comprising an emulsifiable polyisocyanate comprising,
a liquid vehicle and the reaction product of polymeric methane diphenyl diisocyante, a polyisocyanate derived adduct wherein the adduct is the reaction product of a urethane prepolymer formed by reacting a urethane prepolymer of polyoxyethylene-polyoxypropylene glycol and 4,4-diphenylmethane diisocyanate with a first methoxypolyoxyethylene monol having a molecular weight of about 550, polyoxypropylene glycol and a scond methoxypolyoxyethylene monol having a molecular weight of about 750.
52. The emulsion of claim 51 wherein the liquid vehicle is selected from water and castor oil.
53. The emulsion of claim 52 wherein the polymeric methane diphenyl diisocyante is present in an amount of about 93%, the polyisocyanate derived adduct is present in an amount of about 3%, the polyoxypropylene glycol is present in an amount of about 1.5%, and the second methoxypolyoxyethylene monol is present in an amount of about 2.5%.
54. The emulsion of claim 53 wherein the polyoxypropylene glycol has a molecular weight of abut 725.
55. The emulsion of claim 54 wherein the liquid vehicle is water.
56. The emulsion of claim 54 wherein the liquid vehicle is castor oil.