1. A composition for electrophoresis of nucleic acids, the buffer comprising:
(i) TAPS, TAPSO, or Asparagine;
(ii) a compound having a structure of Formula I, wherein:
HO(CH2)m3C\u2014N(CH2)n-OH2\u2003\u2003Formula I
wherein m is an integer of from 1 to 3, and n is an integer of form 1 to 4; and
(iii) a metal-chelating agent.
2. The composition of claim 1, wherein said metal-chelating agent is EDTA.
3. The composition of claim 1, wherein the composition is substantially detergent-free.
4. The composition of claim 1, having a pH greater than 7.
5. The composition of claim 1 comprising TAPS and the compound of Formula I.
6. The composition of claim 5, wherein said metal-chelating agent includes EDTA.
7. The composition of claim 5, wherein the composition is substantially detergent-free.
8. The composition of claim 5, wherein the compound of Formula I is present at a concentration of 50-200 mM, wherein TAPS is present at a same concentration as the compound of Formula I, and wherein the EDTA is present at 1-2 mM.
9. The composition of claim 5, wherein the compound of Formula I is present at a concentration of 50 mM and the EDTA is present at a concentration of 2 mM.
10. The composition of claim 5, having a pH no less than 7.
11. The composition of claim 1, further comprising one or more organic polymers.
12. The composition of claim 11, wherein the one or more organic polymers comprise a sieving component comprising a non-crosslinked acrylamide polymer, and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide, N-substituted polyacrylamide, N-monosubstituted polyacrylamides, polymethacrylamide, polyvinylpyrrolidone, and poly(N,Ndimethylacrylamide).
13. The composition of claim 11, wherein the one or more organic polymers comprise a sieving component comprising one or more polymers selected from the group consisting of linear polyacrylamide, branched acrylamide polymers, and star-shaped acrylamide polymers.
14. An apparatus for resolving samples, comprising:
an electrophoretic channel extending and communicating between anodic and cathodic buffer chambers; and
a buffer, wherein said buffer comprises:
a) a compound having a structure of Formula I, wherein:
HO(CH2)m3C\u2014N(CH2)n\u2014OH2\u2003\u2003Formula I
m is an integer of from 1 to 3, and n is an integer of form 1 to 4; Bis Tris,
b) one or both of TAPS and TAPSO; and
c) a metal-chelating agent.
15. The apparatus of claim 14, wherein the channel is defined by a capillary tube.
16. The apparatus of claim 14, wherein the compound of Formula I is contained in said anodic and cathodic buffer chambers and in said channel.
17. The apparatus of claim 14, further comprising a sieving medium held in said channel.
18. (canceled)
19. A method of electrophoresis of nucleic acids, comprising:
(a) adding a buffer into an electrophoretic channel, wherein said buffer comprises:
i) TAPS;
ii) a metal-chelating agent; and
iii) a compound having a structure of Formula I, wherein:
HO(CH2)m3C-N(CH2)n\u2014OH2\u2003\u2003Formula I
m is an integer of from 1 to 3, and n is an integer of form 1 to 4;
(b) adding a sample including nucleic acids to be analyzed into said electrophoretic channel; and
(c) applying an electromotive potential across the electrophoretic channel.
20. The method of claim 19, wherein the channel is defined by a capillary tube.
21. The method of claim 20, wherein during the application of the electromotive potential, the buffer has a pH of no less than 7.5.
22.-42. (canceled)
43. The composition of claim 5, wherein the compound of Formula I has the structure wherein m is 1 and n is 2.
44. The method of claim 19, wherein the electrophoretic channel contains a sieving medium comprising one or organic polymers.
45. The method of claim 44, wherein the one or more organic polymers comprise a non-crosslinked acrylamide polymer, and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide, N-substituted polyacrylamide, N-monosubstituted polyacrylamides, polymethacrylamide, polyvinylpyrrolidone, and poly(N,Ndimethylacrylamide).
46. The method of claim 44, wherein the one or more organic polymers comprise one or more polymers selected from the group consisting of linear polyacrylamide, branched acrylamide polymers, and star-shaped acrylamide polymers.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A voice coil motor assembly comprising:
a housing comprising a top surface;
a voice coil motor received in the housing;
a cover secured to the voice coil motor and resisting the top surface of the housing, wherein the cover comprises a top surface defining an aperture, a bottom surface opposite to the top surface of the cover, a limiting element protruding from the top surface of the cover, and a frame formed around the brim of the bottom surface of the cover; the frame comprises a top surface and a bottom surface, the top surface of the frame resists the top surface of the housing, and the bottom surface of the frame resists the voice coil motor; and
a transparent board adhering to the top surface of the cover and covering the aperture, the limiting element engaging with the transparent board and configured for restraining movement of the transparent board.
2. The assembly as described in claim 1, wherein the distance between the bottom surface of the frame and the bottom surface of the cover is equal to or greater than the greatest movement distance of the voice coil motor.
3. The assembly as described in claim 1, wherein the voice coil motor comprises a bracket, a barrel, and an elastic element, the barrel is moveably received in the bracket, and the elastic element is secured to a top of the barrel.
4. The assembly as described in claim 3, wherein four positioning posts protrude from a top of the bracket, and the frame of the cover defines four postholes, the positioning posts are engaged in the postholes to secure the cover to the bracket.
5. The assembly as described in claim 3, wherein four fixing posts protrude from a top of the barrel, and the elastic element defines four fixing holes, the fixing posts are engaged in the fixing holes to secure the elastic element to the top of the barrel.
6. The assembly as described in claim 5, wherein the bottom surface of the cover defines two first recessed portions and two second recessed portions, each of the first recessed portions spatially corresponds to one of two opposite fixing holes, and each of the second recessed portions spatially corresponds to one of the other fixing holes.
7. The assembly as described in claim 5, wherein each of the first recessed portions extends through the cover.
8. The assembly as described in claim 3, wherein a pair of latching protrusions protrude from each of opposite sidewalls of the bracket, and each of the opposite sidewalls of the housing defines a pair of through holes, each of the latching protrusions extends through each of the through holes and is latchingly engaged with the through hole to secure the voice coil motor to the housing.
9. The assembly as described in claim 1, wherein the top surface of the housing defines a receiving space receiving the voice coil motor, and the limiting element is exposed to the housing via the receiving space.
10. The assembly as described in claim 1, wherein the shape of the transparent board conforms to the limiting element.