1. A transgenic non-human animal comprising cells containing a DNA sequence encoding a human tissue factor, said sequence operatively linked to a promoter endogenous to the non-human animal, wherein said non-human animal exhibits a human tissue factor phenotype.
2. The transgenic non human animal of claim 1, wherein said non human animal is a mouse.
3. The transgenic mouse of claim 2, wherein said mouse is homozygous for said transgene.
4. The transgenic mouse of claim 1, wherein said mouse is heterozygous for said transgene.
5. The transgenic mouse of claim 1 wherein the mouse does not express endogenous murine tissue factor.
6. A method of identifying a therapeutic agent for use in treating a tissue factor-related disease, comprising:
(a) administering a compound to the transgenic mouse of claim 1, and
(b) screening said transgenic mouse for an improved response associated with a human tissue factor phenotype of said transgenic mouse, thereby identifying a therapeutic agent for use in treating said tissue factor related disease.
7. The method of claim 6, wherein said mouse is homozygous for said transgene.
8. The method of claim 6, wherein said mouse is heterozygous for said transgene.
9. A cell isolated from the transgenic mouse of claim 1, wherein said cell expresses said human tissue factor polypeptide.
10. The mouse cell of claim 9, wherein said mouse is homozygous for said transgene.
11. The mouse cell of claim 9 wherein said mouse is heterozygous for said transgene.
12. A method of identifying a potential therapeutic agent for use in treating a tissue factor related disease, comprising:
(a) contacting a cell of claim 9, said cell containing a DNA construct comprising a DNA sequence encoding a tissue factor polypeptide, with a compound, and
(b) screening said cell to identify a compound having activity that alters a phenotype associated with human tissue factor polypeptide expression, thereby identifying a potential therapeutic agent for use in treating said tissue factor related disease.
13. The method of claim 12, wherein said DNA construct comprises a DNA sequence encoding a green fluorescent protein fusion with said human tissue factor polypeptide.
14. A transgenic mouse comprising cells containing a DNA sequence encoding a human tissue factor, said sequence operatively linked to a murine tissue factor promoter.
15. The transgenic mouse of claim 14, wherein said mouse is homozygous for said transgene.
16. The transgenic mouse of claim 14, wherein said mouse is heterozygous for said transgene.
17. A method of identifying a therapeutic agent for use in treating a human tissue factor disease, comprising:
(a) administering a compound to the transgenic mouse of claim 13 and
(b) screening said transgenic mouse for an improved response associated with a human tissue factor phenotype of said transgenic mouse, thereby identifying a therapeutic agent for use in treating said human tissue factor disease.
18. The method of claim 17 wherein said mouse is homozygous for said transgene.
19. The method of claim 17 wherein said mouse is heterozygous for said transgene.
20. A cell isolated from the transgenic mouse of claim 14, wherein said cell expresses a human tissue factor polypeptide.
21. A method of identifying a potential therapeutic agent for use in treating a human tissue
factor disease, comprising:
(a) contacting a cell of claim 20, said cell containing a DNA construct comprising a DNA sequence encoding human tissue factor polypeptide, with a compound, and
(b) screening said cell to identify a compound having activity that alters a phenotype associated with human tissue factor polypeptide expression, thereby identifying a potential therapeutic agent for use in treating said human tissue factor disease.
22. A DNA construct comprising a DNA sequence encoding a human tissue factor polypeptide, operationally linked to a promoter element.
23. A vector comprising the DNA construct of claim 22.
24. An isolated mouse cell comprising the DNA construct of claim 22.
25. A DNA construct comprising a DNA sequence encoding a fusion polypeptide of human tissue factor polypeptide and green fluorescent protein, operationally linked to a cell-expression element.
26. The DNA construct of claim 25, wherein said human tissue factor polypeptide comprises the amino acid sequence referenced as SEQ ID NO. 1.
27. A mouse embryonic stem cell containing a DNA construct comprising a DNA sequence encoding a human tissue factor polypeptide, said sequence operatively linked to a promoter, and said human tissue factor polypeptide expressed in said embryonic stem cell.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A tester apparatus comprising:
a first printed circuit board;
a pogo block electrically connected to the first printed circuit board on a first side of the pogo block;
wherein in a first configuration the pogo block is configured to be electrically connected to a packaged integrated circuit under test; and
wherein in a second configuration the pogo block is electrically connected on a second side of the pogo block to a second printed circuit board.
2. The tester apparatus as recited in claim 1 further comprising:
a first frame coupled to support the pogo block to form a pogo block assembly in the second configuration; and
the second printed circuit board, wherein the second printed circuit board provides a translation between the first printed circuit board for testing a device with at least one of a different package size or different electrical contacts than in the second configuration.
3. The tester apparatus as recited in claim 2 further comprising:
a second frame coupled to support the pogo block in the first configuration, the second frame being thicker than the first frame.
4. The tester apparatus as recited in claim 3, wherein the first configuration further comprises an alignment plate coupled to the pogo block, the alignment plate to align a package integrated circuit to be tested with electrical connections of the pogo block.
5. The tester apparatus as recited in claim 4 further comprising a plurality of screws securing the alignment plate to the pogo block.
6. The tester apparatus as recited in claim 4 further comprising a plurality of screws securing the alignment plate and pogo block to the second frame.
7. The tester apparatus as recited in claim 3 where the first frame has a thickness to allow pogo pins to make electrical contact with the second printed circuit board.
8. The tester apparatus as recited in claim 2, wherein the first frame coupled with the pogo block includes a space allowing a component to protrude from the first printed circuit board into a space between the frame and the pogo block.
9. The apparatus as recited in claim 8 further comprising a component coupled to the base printed circuit board extending into the space formed by the first frame coupled with the contactor allowing the component to protrude from the first printed circuit board into the space adjacent to the frame and the pogo block.
10. The tester apparatus as recited in claim 1 where the pogo block is generally X-shaped.
11. The tester apparatus as recited in claim 1 wherein the pogo block comprises:
a pogo pin retainer portion;
a plurality of pogo pins; and
a portion to receive the pogo pins held in by the pogo pin retainer portion.
12. The tester apparatus as recited in claim 1, wherein the second configuration further comprises an alignment plate coupled to the pogo block, the alignment plate to align a package integrated circuit to be tested with electrical connections of the pogo block.
13. A tester apparatus comprising:
a first printed circuit board;
wherein in a first configuration a pogo block assembly including a plurality of pogo blocks and a first frame, is configured to be electrically connected to the first printed circuit board and to receive a packaged integrated circuit under test; and
wherein in a second configuration the plurality of pogo blocks are coupled to a second frame, thinner then the first frame, and electrically connected to the first printed circuit board and electrically connected to a second printed circuit board.
14. The tester apparatus as recited in claim 13 further comprising:
a contactor assembly coupled to the second printed circuit board in the second configuration to receive one or more devices under test.
15. The tester apparatus as recited in claim 13 wherein in the second configuration, the second printed circuit board provides a translation from a first number of sites on the first printed circuit board to a second number of sites on the second printed circuit board.
16. The tester apparatus as recited in claim 15 wherein the first number of sites and the second number of sites are equal.
17. The tester apparatus as recited in claim 2 wherein the first number of sites is more than the second number of sites.
18. The tester apparatus as recited in claim 2 wherein the first configuration provides for testing a first package type and the second configuration provides for testing a second package type.
19. A method of configuring a tester comprising:
configuring the tester in a first or a second configuration, wherein
configuring the tester in the first configuration includes,
securing a pogo block to a first frame and an alignment plate to form a first contactor assembly, the alignment plate to align a packaged integrated circuit under test; and
electrically connecting the pogo block to a first printed circuit board;
wherein configuring the tester in a second configuration includes, securing the pogo block to a second frame thinner than the first frame;
electrically coupling the first printed circuit board to one side of the pogo block and electrically connecting a second printed circuit board to another side of the pogo block; and
coupling a contactor assembly including another pogo block, frame, and alignment plate to the second printed circuit board.
20. The method as recited in claim 19 wherein the first contactor assembly is for a first package type and the second contactor assembly is for a second package type.