What is claimed is:
1. A dry powder nucleic acid composition comprising insoluble nucleic acid constructs dispersed within a hydrophilic excipient material.
2. A dry powder nucleic acid composition as in claim 1, wherein the composition consists essentially of particles of the nucleic acid constructs dispersed within the hydrophilic excipient material, present in a powder of the excipient material.
3. A dry powder nucleic acid composition as in claim 2, wherein the nucleic acid construct particles have an average particle size in the range from 0.5 m to 50 m.
4. A dry powder nucleic acid composition as in claim 1, wherein the nucleic acid constructs comprise bare nucleic acid molecules or viral vectors.
5. A dry powder nucleic acid composition as in claim 1, wherein the nucleic acid constructs comprise nucleic acids present in a vesicle, wherein the vesicle is dispersed within the hydrophilic excipient.
6. A dry powder nucleic acid composition as in claim 1, wherein nucleic acid construct includes a structural region and a regulatory region.
7. A dry powder nucleic acid composition as in claim 1, wherein the hydrophilic excipient is a material selected from the group consisting of inorganic salts, sugars, sugar alcohols, oligosaccharides, amino acids, organic acids and salts, carbohydrates, proteins, and peptides.
8. A method for preparing dry powder nucleic acid compositions, said method comprising:
suspending insoluble nucleic acid constructs in an aqueous solution of a hydrophilic excipient; and
drying the solution to produce a powder comprising particles of the nucleic acid constructs dispersed within the dried excipient material.
9. A method as in claim 8, wherein the nucleic acid constructs are present in the aqueous solution at a weight ratio in the range form 2:1 to 1:100 nucleic acid construct: hydrophilic excipient.
10. A method as in claim 8, wherein the aqueous solution is dried by spraying droplets into a gas stream, wherein particles having a size in the range from 0.5 to 50 m are produced.
11. A method as in claim 8, wherein the aqueous solution is dried by exposure to a vacuum to produce a crude powder, further comprising grinding the crude powder to produce a final powder size in the range from 1 to 50 m.
12. A method as in claim 8, wherein the nucleic acid constructs comprise bare nucleic acid molecules or viral vectors and the aqueous solution is buffered.
13. A method as in claim 8, wherein the nucleic acid constructs comprise nucleic acids present in a vesicle and the aqueous solution is substantially free from buffer and salts.
14. A method as in claim 8, wherein the nucleic acid constructs include a structural region and a regulatory region.
15. A method as in claim 8, wherein the hydrophilic excipient is a material selected from the group consisting of inorganic salts, sugars, sugar alcohols, oligosaccharides, amino acids, organic acids and salts, carbohydrates, proteins, and peptides.
16. A method for delivering nucleic acid constructs to a moist target location in a patient, said method comprising
dispersing a dry powder comprising particles of insoluble nucleic acid constructs in a hydrophilic excipient material in a gas stream; and
directing the gas stream at the moist target location, whereby the hydrophilic excipient coating absorbs water and dissolves to release the nucleic acid constructs.
17. A method as in claim 16, wherein the target location is the lung and the gas stream is directed to the lung by inhalation.
18. A method as in claim 17, wherein the coated nucleic acid constructs have a particle size in the range from 0.5 m to 50 m.
19. A method as in claim 16, wherein the nucleic acid constructs comprise bare nucleic acid molecules or viral vectors.
20. A method as in claim 16, wherein the nucleic acid constructs comprise nucleic acids present in a vesicle.
21. A method as in claim 16, wherein the nucleic acid constructs include a structural region and a regulatory region.
22. A method as in claim 16, wherein the hydrophilic excipient is a material selected from the group consisting of inorganic salts, sugars, sugar alcohols, oligosaccharides, amino acids, organic acids and salts, carbohydrates, proteins, and peptides.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An electrical connector adapted for being mounted onto a printed circuit board defining a plurality of vias, the electrical connector comprising:
an insulative housing having a mounting surface adapted for being mounted onto the printed circuit board;
a contact module assembled to the insulative housing, said contact module comprising a plurality of conductive terminals, each of the conductive terminals comprising a pin end extending beyond the mounting surface for being inserted into one of the vias;
a position fixer having a plurality of through holes each retaining to a corresponding pin end prior to the position fixer being moved up to the mounting surface when the electrical connector is mounted to the printed circuit board.
2. The electrical connector as claimed in claim 1, wherein said pin end comprises a needle eye portion having a first end and a second end, and the position fixer, prior to the electrical connector being mounted onto the printed circuit board, is disposed adjacent to the first end while, after the electrical connector being pressed into the printed circuit board, the position fixer is moved to the second end.
3. The electrical connector as claimed in claim 2, wherein a diameter of the needle eye portion is greater than a diameter of the through hole of the position fixer.
4. The electrical connector as claimed in claim 1, wherein there are provided plural contact modules assembled to the insulative housing, and said insulative housing defines a plurality of ports forwardly opening to receive a plurality of mating connectors, respectively.
5. The electrical connector as claimed in claim 1, wherein said position fixer is parallel to the printed circuit board.
6. The electrical connector as claimed in claim 1, wherein said pin ends extend along a direction perpendicular to the position fixer.
7. The electrical connector as claimed in claim 1, wherein said insulative housing comprises a projection portion for aligning to the printed circuit board.
8. The electrical connector as claimed in claim 1, further comprising a sub-circuit board at the mounting surface of the insulative housing.
9. The electrical connector as claimed in claim 1, further comprising a shielding shell enclosing the insulative housing, the shielding shell having a plurality of ground tails.
10. A method of mounting an electrical connector having a housing and a plurality of conductive terminals to a printed circuit board having a plurality of vias, the method comprising the steps of:
retaining a position fixer defining a plurality of through holes corresponding to the conductive terminals to pin ends of the conductive terminals; and
inserting the pin ends into the vias while moving the position fixer all the way up to the housing.
11. The method of assembling an electrical connector as claimed in claim 10, further comprising a step of providing a needle eye portion to the pin end, and wherein the step of retaining comprises placing the position fixer onto the needle eye portion.
12. An electrical connector for mating with at least a complementary connector and for mounting to a printed circuit board, comprising:
an insulative housing defining a mating face for mating with said at least complementary connector, and a mounting face for mounting to the printed circuit board;
a plurality of contact modules dispose in the housing around the mounting face, said contact module including a position member associated with a plurality of conductive terminals, each of said terminal defining a press-fit mounting portion for mounting to a corresponding hole in the printed circuit board, said press-fit mounting portion including an upper needle eye section and a lower pin type section wherein said upper needle eye section is transversely shrinkable and defines a larger width than the lower pin type section; and
a position fixer characterized with deformability thereof; wherein
the housing and the contact modules are configured to allow the lower pin type sections of said press-fit mounting portions to pierce through said positioning fixer and retained thereto in a snug manner when said position fixer is located in a lower position before said connector is mounted to the printed circuit board, and to allow the upper needle eye sections of said mounting portions to further pierce through said position fixer via a procedure of mounting said connector to the printed circuit board to have the upper needle eye sections further inserted into said corresponding holes in the printed circuit board when said position fixer is located in a final position.
13. The electrical connector as claimed in claim 12, wherein said position fixer is moved above the needle eye section when the position fixer is located in the final position.
14. The electrical connector as claimed in claim 12, wherein said position member is wholly located around said mounting face and equipped with two rows of said terminals arranged in a minor image with a shielding plate therebetween.
15. The electrical connector as claimed in claim 12, wherein the shielding plate defines a height similar to that of the housing while the position member is less than a half of that of the housing.
16. The electrical connector as claimed in claim 12, wherein a sub-circuit board is mounted between the contact module and the position fixer and pierced by the mounting portion.