1-2. (canceled)
3. A process for the treatment of oil spills, soil remediation, degreasing and release of oil in the automotive industry, and oil dispersal and equipment clean-up in the oil transport, aerospace and food preparation industries comprising:
providing a composition which includes 7.3%-9.3% by weight of cocoamidopropyl-betaine; 6.8%-8.3% by weight of tall oil fatty acid; 7.9%-9.7% by weight monoethanolamine; 7.9%-9.7% by weight of a Nonionic Octylphenol Ethoxylate having the formula:
wherein R=octyl (C8) and x=4.5 (avg); 8.3%-10.4% by weight of a Nonionic Octylphenol Ethoxylate having the formula:
wherein R=octyl (C8) and x=9.5 (avg); 8.4%-10.5% by weight of a tetrasodium salt of ethylenediaminetetraacetic acid; and water to make 100% by weight; and
contacting an oil spill, soil in need of remediation, automotive components requiring degreasing or release of oil, and equipment in need of clean-up from the oil transport, aerospace and food preparation industries with said composition;
whereby oil spill treatment, soil remediation, degreasing and release of oil in the automotive industry, and oil dispersal and equipment clean-up in the oil transport, aerospace and food preparation industries are effected.
4. A process for the treatment of oil spills, soil remediation, degreasing and release of oil in the automotive industry, and oil dispersal and equipment clean-up in the oil transport, aerospace and food preparation industries comprising:
providing a composition which includes 8.87% by weight of cocoamidopropyl-betaine; 7.74% by weight of tall oil fatty acid; 8.68% by weight monoethanolamine; 8.87% by weight of a Nonionic Octylphenol Ethoxylate having the formula:
wherein R=octyl (C8) and x=4.5 (avg); 9.06% by weight of a Nonionic Octylphenol Ethoxylate having the formula:
wherein R=octyl (C8) and x=9.5 (avg); 9.15% by weight of a tetrasodium salt of ethylenediaminetetraacetic acid; and 47.63% by weight water; and
contacting an oil spill, soil in need of remediation, automotive components requiring degreasing or release of oil, and equipment in need of clean-up from the oil transport, aerospace and food preparation industries with said composition;
whereby oil spill treatment, soil remediation, degreasing and release of oil in the automotive industry, and oil dispersal and equipment clean-up in the oil transport, aerospace and food preparation industries are effected.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method of assaying in a microfluidic device the amount of an analyte in a biological fluid, said microfluidic device having at least one sample inlet port, at least one air vent, and at least one reagent-containing chamber, comprising:
(a) dispensing a sample of said biological fluid into said at least one sample inlet port of said microfluidic device, said sample moving by capillary force to a capillary stop through a capillary passageway communicating with said at least one sample inlet;
(b) dispensing into said at least one inlet port of (a) a portion of a liquid different from said sample of (a), said liquid portion being sufficient to force said sample past said capillary stop, said liquid portion being dispensed in the form of a group of droplets having diameters in the range of 0.05 to 1 mm, said groups of droplets being separated by intervals when no droplets are dispensed, said liquid portion being added at a predetermined time after introducing said sample.
2. A method of claim 1 wherein said different liquid of (b) is introduced in an amount sufficient to displace all of said sample of (a) to a position in said microfluidic device beyond said capillary stop.
3. A method of claim 2 wherein said displaced sample of claim 2 contacts a reagent dispensed in said at least one reagent-containing chamber and displaces air found in said at least one chamber.
4. A method of claim 3 wherein said sample and said reagent react and produce a detectable result related to the amount of said analyte in said sample.
5. A method of claim 2 wherein said displaced sample contacts a conditioning agent or a carrier agent to prepare said sample for subsequent contact with a reagent.
6. A method of claim 1 wherein said groups of droplets of said different liquid of (b) are dispensed by a micro-dispensing nozzle at a rate of about 30 to 150 thousand drops per second.
7. A method of claim 1 wherein said microfluidic device has a total volume of about 0.1 to 200 \u03bcL.
8. A method of claim 1 wherein the smallest group of droplets have a volume of about 100 pL.
9. In a method of assaying in a microfluidic device the amount of an analyte in a biological fluid, said microfluidic device having at least one sample inlet port, at least one air vent, and at least one reagent-containing chamber, said assay comprising dispensing a sample of said biological fluid into said at least one inlet port and displacing said sample by dispensing a liquid different from said sample into said at least one inlet port, the improvement comprising dispensing said different liquid in the form of group of droplets having diameters in the range of 0.05 to 1 mm, said groups of droplets being separated by intervals when no droplets are dispensed.
10. A method of claim 9 wherein said sample of biological fluid moves by capillary force to a capillary stop in a capillary passageway communicating with said at least one sample inlet and said different liquid is dispensed in an amount sufficient to force said sample past said capillary stop.
11. A method of claim 10 wherein said sample of biological fluid is forced past said capillary stop into said at least one reagent-containing chamber.
12. A method of claim 10 wherein said sample and said reagent react and produce a detectable result related to the amount of said analyte in said sample.
13. A method of claim 10 wherein said sample contacts a conditioning agent or a carrier agent to prepare said sample for subsequent contact with a reagent.
14. A method of claim 9 wherein said groups of droplets of said different liquid are dispensed by a micro-dispensing nozzle at a rate of about 30 to 150 thousand drops per second.
15. A method of claim 9 wherein said microfluidic device has a total volume of about 0.1 to 200 \u03bcL.
16. A method of claim 9 wherein the smallest group of droplets has a volume of about 100 pL.
17. A method of claim 10 wherein the volume of different liquid dispensed is about 5nL.
18. A method of operating a microfluidic device, said microfluidic device having at least one inlet port, at least one air vent, and at least one chamber, comprising:
(a) dispensing into said at least one inlet port a predetermined amount of a first liquid in the form of groups of droplets having diameters in the range of 0.05 to 1 mm;
(b) dispensing into said at least one inlet port a predetermined amount of a second liquid sufficient to force said first liquid from said at least one inlet port, said second liquid being dispensed in the form of a group of droplets having diameters in the range of 0.05 to 1 mm, said groups of droplets being separated by intervals when no droplets are dispensed, said second liquid being added at a predetermined time after introducing said first liquid.
19. A method of claim 18 wherein said microfluidic device comprises a capillary passageway communicating between said at least one inlet port, and said at least one chamber, said first liquid moving by capillary force from said inlet port to a capillary stop at the entrance to said at least one chamber.