1. An inkpot limiting device for limiting a position of an inkpot, the inkpot limiting device comprising:
a limiting structure;
a shaft, wherein the limiting structure is located in a diameter direction of the shaft;
a carrier comprising a main body portion, a carrying portion, and a limiting portion, wherein the carrying portion and the limiting portion are located at opposite sides of the main body portion, the shaft passes through the main body portion in a way that the carrier is rotatable around the shaft, and the limiting structure and the limiting portion face each other; and
a pair of limiting magnets comprising:
a permanent magnet disposed on one of the limiting structure and the limiting portion; and
an electromagnet disposed on the other one of the limiting structure and the limiting portion, wherein when an electricity is applied, the electromagnet is magnetically attracted to fix with the permanent magnet.
2. The inkpot limiting device according to claim 1, wherein the limiting portion is a C shape and the limiting structure is extended and located in an opening of the C-shaped limiting portion.
3. The inkpot limiting device according to claim 1, wherein the limiting structure is disposed in a housing of a multifunctional printer.
4. The inkpot limiting device according to claim 1, wherein the limiting structure is one of a guide rail or an introducer while the limiting portion is the other one of the guide rail or the introducer.
5. The inkpot limiting device according to claim 1, wherein when the electricity is not applied, the electromagnet and the permanent magnet are not attracted to each other by a magnetic force, and the carrier is adapted to move along an axis direction of the shaft.
6. A multifunctional printer comprising:
a housing;
an inkpot limiting device disposed in the housing and comprising:
a limiting structure disposed on the housing;
a shaft, wherein the limiting structure is located in a diameter direction of the shaft;
a carrier comprising a main body portion, a carrying portion, and a limiting portion, wherein the carrying portion and the limiting portion are located at opposite sides of the main body portion, the shaft passes through the main body portion in a way that the carrier is rotatable around the shaft, and the limiting structure and the limiting portion face each other; and
a pair of limiting magnets comprising:
a permanent magnet disposed on one of the limiting structure and the limiting portion; and
an electromagnet disposed on the other one of the limiting structure and the limiting portion, wherein when an electricity is applied, the electromagnet is magnetically attracted to fix with the permanent magnet.
7. The multifunctional printer according to claim 6, wherein the limiting portion is a C shape and the limiting structure is extended and located in an opening of the C-shaped limiting portion.
8. The multifunctional printer according to claim 6, wherein the limiting structure is one of a guide rail or an introducer, and the limiting portion is the other one of the guide rail or the introducer.
9. The multifunctional printer according to claim 6, wherein when the electricity is not applied, the electromagnet and the permanent magnet are not attracted to each other by a magnetic force, and the carrier is adapted to move along an axis direction of the shaft.
10. The multifunctional printer according to claim 6, further comprising a scraper, wherein when the electricity is applied and the electromagnet is magnetically attracted to fix with the permanent magnet, a distance between a bottom surface of the carrier and a scraper in a perpendicular direction is maintained at a fixed value, and the perpendicular direction is perpendicular to the axis direction of the shaft.
11. The multifunctional printer according to claim 6, further comprising an inkpot disposed on the carrying portion of the carrier.
12. The multifunctional printer according to claim 11, further comprising a scraper, wherein when the electricity is applied and the electromagnet is magnetically attracted to fix with the permanent magnet, a distance between a bottom surface of a tip of the inkpot and the scraper in a perpendicular direction is maintained at a fixed value, and the perpendicular direction is perpendicular to the axis direction of the shaft.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. Nucleic acid encoding a polypeptide with the bioactivity of the ultraspiracle protein, comprising a sequence selected from
(a) the sequence of SEQ ID NO: 1,
(b) sequences which have at least 85% identity with the sequence of SEQ ID NO: 1 over a length of at least 600 consecutive nucleotides,
(c) sequences which, owing to the degeneracy of the genetic code, encode the same amino acid sequence as the sequences defined under (a) and (b),
(d) parts of the sequences as defined under (a), (b) and (c) which encode polypeptides which have essentially the same bioactivity as a polypeptide with the amino acid sequence of SEQ ID NO: 2.
2. Vector comprising at least one nucleic acid according to claim 1.
3. Vector according to claim 2, characterized in that the nucleic acid molecule is linked functionally to regulatory sequences which ensure the expression of the nucleic acid in pro- or eukaryotic cells.
4. Host cell containing a nucleic acid according to claim 1 or a vector according to claim 2 or 3.
5. Host cell according to claim 4, characterized in that it is a pro- or eukaryotic cell.
6. Host cell according to claim 5, characterized in that the prokaryotic cell is E. coli.
7. Host cell according to claim 5, characterized in that the eukaryotic cell is a yeast cell, mammalian cell, insect cell or plant cell.
8. Transgenic organism, with the exception of humans, containing a nucleic acid according to claim 1 or a vector according to claim 2 or 3.
9. Polypeptide which is encoded by a nucleic acid according to claim 1.
10. Receptor comprising an EcR subunit and a polypeptide according to claim 9.
11. Antibody which binds specifically to a polypeptide according to claim 9.
12. Process for the preparation of a polypeptide according to claim 9, comprising the following steps:
(a) culturing a host cell according to one of claims 4 to 7 under conditions which ensure the expression of the nucleic acid according to claim 1, and
(b) obtaining the polypeptide from the cells or the culture medium.
13. Process for the preparation of a nucleic acid according to claim 1, comprising the following steps:
(a) complete chemical synthesis in a manner known per se or
(b) chemically synthesizing oligonucleotides, labelling the oligonucleotides, hybridizing the oligonucleotides with DNA of an insect cDNA library, selecting positive clones and isolating the hybridizing DNA from positive clones, or
(c) chemical synthesis of oligonucleotides and amplification of the target DNA by means of PCR.
14. Regulatory region which naturally controls the transcription of a nucleic acid according to claim 1 in insect cells and which ensures specific expression.
15. Method of finding new active compounds for crop protection, in particular compounds which cause the activation or inhibition of a polypeptide according to claim 9 or a receptor according to claim 10, comprising the following steps:
(a) providing a host cell according to one of claims 4 to 7,
(b) culturing the host cell in the presence of a chemical or a mixture of chemicals, and
(c) detecting the activation or inhibition of the polypeptide or receptor.
16. Method of finding a compound which binds to a polypeptide according to claim 9, comprising the following steps:
(a) contacting a polypeptide according to claim 9 with a compound or a mixture of compounds under conditions which permit the interaction of the compound(s) with the polypeptide, and
(b) identifying the compound which binds specifically to the polypeptide.
17. Method for inducibly expressing target genes by means of a polypeptide according to claim 9, comprising the following steps:
(a) culturing a host cell according to one of claims 4 to 7 or providing a transgenic organism according to claim 8 under conditions which ensure the expression of the nucleic acid according to claim 1, where the host cell or the transgenic organism contains a target gene with suitable regulatory sequences, and
(b) contacting the host cell or the transgenic organism with a chemical which induces the expression of the target gene.
18. Use of at least one nucleic acid according to claim 1, of a vector according to claim 2 or 3, of a host cell according to one of claims 4 to 7, of a transgenic organism according to claim 8, of a polypeptide according to claim 9, of a receptor according to claim 10 or of a regulatory region according to claim 14 for finding new active compounds for crop protection.
19. Use of at least one nucleic acid according to claim 1, of a vector according to claim 2 or 3, of a host cell according to one of claims 4 to 7, of a transgenic organism according to claim 8, of a polypeptide according to claim 9, of a receptor according to claim 10, of a regulatory region according to claim 14 or of a method according to claim 17 for the directed modification of the biological properties of a host cell or a host organism.