Author: claimsbot

1. A process converting greater than 1,000 Kghour of a feedstock in the presence of a molecular sieve into one or more olefin(s), wherein the feedstock comprises less than 50 ppm of a Group IA metal salt or a Group IIA metal salt.
2. The process of claim 1 wherein the feedstock comprises less than 30 ppm of a Group IA metal salt andor a Group IIA metal salt.
3. The process of claim 1 wherein the feedstock comprises less than 20 ppm of a Group IA metal salt andor a Group IIA metal salt.
4. The process of claim 1 wherein the feedstock comprises less than 10 ppm of a Group IA metal salt andor a Group IIA metal salt.
5. The process of claim 1 wherein the feedstock comprises less than 1 ppm of a Group IA metal salt andor a Group IIA metal salt.
6. The process of claim 1 wherein the feedstock comprises less than 500 ppb of a Group IA metal salt andor a Group IIA metal salt.
7. The process of claim 1 wherein the feedstock comprises an oxygenate.
8. The process of claim 1 wherein one or more olefin(s) are produced at a rate of 1,000 Kghour.
9. The process of claim 1 wherein the molecular sieve is synthesized from the combination from at least one, preferably at least two, of the group consisting of a silicon source, a phosphorous source and an aluminum source, optionally in the presence of a templating agent.
10. A process for converting a feedstock in the presence of a molecular sieve in a reactor, the process comprising the steps of: (a) introducing to the reactor a feedstock at a rate of greater than 1000 Kg per hour, wherein the feedstock comprises less than 50 ppm of a Group IA metal salt andor a Group IIA metal salt; (b) introducing a molecular sieve to the reactor; and (c) withdrawing an effluent stream from the reactor, the effluent stream comprising greater than 1,000 Kg of one or more olefin(s) per day.
11. The process of claim 10 wherein rate of introducing the feed stock is greater than 10,000 Kghour.
12. The process of claim 10 wherein the feedstock comprises less than 30 ppm of a Group IA metal salt or a Group IIA metal salt.
13. The process of claim 10 wherein the feedstock comprises less than 20 ppm of a Group IA metal salt or a Group IIA metal salt.
14. The process of claim 10 wherein the feedstock comprises less than 10 ppm of a Group IA or a Group IIA metal salt.
15. The process of claim 1 wherein the feedstock comprises an oxygenate.
16. The process of claim 1 wherein the process is producing greater than 10,000 kg of one or more olefin(s) per hour.
17. The process of claim 10 wherein the process is producing 20,000 kghour of ethylene andor propylene.
18. The process of claim 10 wherein the reactor is a fixed bed reactor.
19. The process of claim 10 wherein the reactor is a fast fluidized bed rector.
20. A process for converting a feedstock in the presence of a molecular sieve catalyst composition in a reactor to produce greater than 1,000 Kg per hour of one or more olefin(s), the process operating substantially free of a Group IA metal salt andor a GroupIIA metal salt.
21. The process of claim 20 wherein the process is operating at greater than 2,000 Kg per hour of ethylene andor propylene.
22. The process of claim 20 wherein the process is operating at greater than 4,000 Kg per hour of ethylene and propylene.
23. The process of claim 20 wherein the Group IA metal salt are salts where the metal is sodium or potassium.
24. The process of claim 20 wherein the rate of feedstock entering the reactor per day is greater than 100,000 kg per day.
25. The process of claim 20 wherein the feedstock comprises less than 20 ppm of the Group I metal salt andor Group IIA metal salt.
26. The process of claim 20 wherein the feedstock comprises less than 10 ppm of the Group IA metal salt andor Group IIA metal salt.
27. The process of claim 20 wherein the reactor is a fast fluidized bed reactor operating at a WHSV in the range of from 1 hr1 to 20 hr1.
28. The process of claim 27 wherein the reactor is at a temperature in the range of from 200 C. to 650 C.
29. The process of claim 20 wherein the feedstock comprises methanol.
30. A process for converting a feedstock, the process comprising the steps of
(a) providing a feedstock comprising greater than 10,000 ppm of a Group IA metal salt andor a Group IIA metal salt;
(b) removing an amount of the Group IA metal salt andor Group IIA metal salt to a level less than 50 ppm to form a treated feedstock;
(c) introducing the treated feedstock into a reactor;
(d) providing a molecular sieve catalyst composition in the reactor to convert the treated feedstock into one or more olefin(s); and
(e) withdrawing an effluent stream comprising the one or more olefin(s) from the reactor.
31. The process of claim 30 wherein the Group IA metal salt andor Group IIA metal salt in step (b) is removed by boiling the feedstock.
32. The process of claim 30 wherein the treated feedstock comprises less than 30 ppm of a Group IA metal salt andor a Group IIA metal salt.
33. The process of claim 30 wherein the treated feedstock comprises less than 20 ppm of a Group IA metal salt andor a Group IIA metal salt.
34. The process of claim 30 wherein the treated feedstock comprises less than 10 ppm of a Group IA metal salt andor a Group IIA metal salt.
35. The process of claim 30 wherein the effluent stream is being remove at a rate greater than 10,000 Kgday.
36. The process of claim 30 wherein the reactor is a fluidized bed reactor.
37. The process of claim 30 wherein the effluent stream comprises greater than 10,000 Kg of olefin(s) per kg of the total effluent stream.
38. The process of claim 30 wherein the treated feedstock is introduced into the reactor at a rate of greater than 1,000 Kghour.
39. The process of claim 30 wherein the treated feedstock is introduced into the reactor at a rate of greater than 10,000 Kghour.
40. A process for converting a feedstock comprising an oxygenate, the process comprising the steps of:
(a) introducing the feedstock to a reactor system in the presence of a molecular sieve catalyst composition comprising a molecular sieve, wherein the amount of a Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 10,000 ppm;
(b) withdrawing from the reactor system an effluent stream;
(c) passing the effluent stream through a recovery system; and
(d) recovering at least one or more olefin(s) in amount greater than 1,000 Kgday.
41. The process of claim 40 wherein the effluent steam is withdrawn at a rate greater than 1,000 Kghour.
42. The process of claim 40 wherein the feedstock is introduced to the reactor at a rate greater than 1,000 Kghour.
43. The process of claim 40 wherein the process is producing greater than 10,000 Kg per day of the one or more olefin(s).
44. The process of claim 40 wherein the amount of a Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 5000 ppm.
45. The process of claim 40 wherein the amount of a Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 1000 ppm.
46. The process of claim 40 wherein the amount of a Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 500 ppm.
47. The process of claim 40 wherein the amount of a Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 250 ppm.
48. The process of claim 40 wherein the amount of a Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 100 ppm.
49. The process of claim 40 wherein the oxygenate comprises methanol and less than 50 ppm of a Group IA andor Group IIA metal salt.
50. An integrated process for making one or more olefin(s), the integrated process comprising the steps of:
(a) passing a hydrocarbon feedstock to a syngas production zone to producing a synthesis gas stream;
(b) contacting the synthesis gas stream with a catalyst to form an oxygenated feedstock; and
(c) converting the oxygenated feedstock containing less than 50 ppm of a Group IA metal salt andor a Group IIA metal salt into the one or more olefin(s) in a reactor in the presence of a molecular sieve catalyst composition.
51. The integrated process of claim 50 wherein the process further comprises the step of: (d) polymerizing the one or more olefin(s) in the presence of a polymerization catalyst into a polyolefin at a rate greater than 1,000 Kghour.
52. The integrated process of claim 50 wherein the process further comprises the step of: (d) polymerizing the one or more olefin(s) in the presence of a polymerization catalyst into a polyolefin at a rate greater than 10,000 Kghour.
53. The integrated process of claim 50 wherein the oxygenated feedstock comprises methanol, the olefin(s) include ethylene and propylene, and the molecular sieve catalyst composition is a silicoaluminophosphate molecular sieve.
54. The integrated process of claim 50 wherein the molecular sieve is synthesized from the combination from at least one of the group consisting of a silicon source, a phosphorous source and an aluminum source, optionally in the presence of a templating agent.
55. The process of claim 50 wherein the feedstock contains less than 30 ppm of a Group IA metal salt.
56. The process of claim 50 wherein the feedstock contains less than 20 ppm of a Group IA metal salt.
57. The process of claim 50 wherein the reactor is a fast fluidized bed reactor.
58. The process of claim 50 wherein the reactor is producing 1000 Kghour of ethylene.
59. The process of claim 50 wherein the reactor is a fixed bed reactor.
60. A process of converting an oxygenated feedstock into one or more olefin(s) in the presence of a molecular sieve catalyst composition comprising a molecular sieve, the process comprising the steps of:
(a) passing a hydrocarbon feedstock to a syngas production zone producing a synthesis gas stream;
(b) contacting the synthesis gas stream with a catalyst to form the oxygenated feedstock;
(c) transporting the oxygenated feedstock containing a first amount of a Group IA metal salt andor a Group IIA metal salt;
(d) removing from the oxygenated feedstock the Group IA metal salt andor the Group IIA metal salt such that feedstock contains a second amount of the Group IA andor Group IIA metal salt to form a treated oxygenated feedstock, wherein the second amount is less than the first amount; and
(e) converting the treated oxygenated feedstock into the one or more olefin(s) in a reactor in the presence of a molecular sieve catalyst composition.
61. The process of claim 60 wherein the process further comprises the step of:
(f) polymerizing the one or more olefin(s) in the presence of a polymerization catalyst into a polyolefin at a rate greater than 1,000 Kghour.
62. The process of claim 60 wherein the first amount of the Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 10,000 ppm.
63. The process of claim 60 wherein the first amount of the Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 1000 ppm.
64. The process of claim 60 wherein the first amount of the Group IA metal salt andor Group IIA metal salt based on the molecular sieve is less than 500 ppm.
65. The process of claim 60 wherein the second amount of the Group IA metal salt andor Group IIA metal salt is less than 50 ppm.
66. The process of claim 60 wherein the second amount of the Group IA metal salt andor Group IIA metal salt is less than 30 ppm.
67. The process of claim 60 wherein the second amount of the Group IA metal salt andor Group IIA metal salt is less than 20 ppm.
68. The process of claim 60 wherein the second amount of the Group IA metal salt andor Group IIA metal salt is less 10 ppm.
69. The process of claim 60 wherein the second amount of the Group IA metal salt andor Group IIA metal salt based is less than 1 ppm.
70. The process of claim 60 wherein the ratio of the first amount to the second amount of the Group IA metal salt andor Group IIA metal salt is greater than or equal to 20.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

We claim:

1. A water soluble composition for dip-coating a substrate comprised of:
a) hydroxypropylmethyl cellulose; and
b) a thickener selected from the group consisting of xanthan gum, carrageenan, and mixtures thereof, wherein the composition possesses a surface gloss of at least 150 when applied via dip coating to a substrate.
2. The composition of claim 1, wherein the composition is comprised of, based upon the total dry weight of the composition,
a) from about 95 percent to less than about 100 percent of hydroxypropylmethyl cellulose; and
b) from about 0.5 percent to about 5 percent of a thickener selected from the group consisting of xanthan gum, carrageenan, and mixtures thereof,.
3. The composition of claim 1, wherein the composition is comprised of, based upon the total dry weight of the composition,
a) from about 95 percent to about 99.5 percent of hydroxypropylmethyl cellulose; and
b) from about 0.5 percent to about 5 percent of xanthan gum.
4. The composition of claim 2 further comprising, based upon the total dry weight of the composition, up to about 40% plasticizers.
5. The composition of claim 4 wherein the plasticizers are selected from the group consisting of polyethylene glycol, glycerin, sorbitol, triethyl citrate, tribuyl citrate, dibutyl sebecate, vegetable oils, surfactants, propylene glycol, mono acetate of glycerol, diacetate of glycerol, triacetate of glycerol, natural gums, and mixtures thereof.
6. The composition of claim 4, wherein the plasticizers are selected from the group consisting of glycerin, polyethylene glycol, propylene glycol, castor oil, and mixtures thereof.
7. The composition of claim 2 further comprising, based upon the total dry weight of the composition, up to about 14% of a coloring agent.
8. The composition of claim 7 wherein the coloring agent is selected from the group consisting of azo dyes, quinopthalone dyes, triphenylmethane dyes, xanthene dyes, indigoid dyes, iron oxides, iron hydroxides, titanium dioxide, natural dyes, and mixtures thereof.
9. A dosage form for delivering an active agent, the form comprising an outer coating, said outer coating comprising the composition of claim 2.
10. A pharmaceutical dosage form comprising an outer coating of the composition of claim 2.
11. A pharmaceutical dosage form comprising a core, a subcoating substantially covering said core, and an outer coating substantially covering said subcoating, wherein the outer coating is comprised of the composition of claim 2.
12. The coated dosage form of claim 11 wherein the subcoating comprises materials selected from the group consisting of cellulose ethers, plasticizers, polycarbohydrates, pigments, opacifiers, and mixtures thereof.
13. The coated dosage form of claim 11 wherein the subcoating comprises materials selected from the group consisting of hydroxypropylmethylcellulose, castor oil, polyethylene glycol, polysorbate 80, maltodextrin, and mixtures thereof.
14. The dosage form of claim 11 wherein the subcoating is comprised of, based upon the total dry weight of the subcoating,
a) from about 2 percent to about 8 percent of a water-soluble cellulose ether selected from the group consisting of hydroxypropylmethylcellulose, hydroxypropylcellulose, hydroxyethylcellulose, and mixtures thereof.
b) from about 0.1 percent to about 1 percent castor oil.
15. The dosage form of claim 11 wherein the subcoating is comprised of, based upon the total dry weight of the subcoating,
a) from about 4 percent to about 6 percent hydroxypropylmethylcellulose; and
b) from about 0.1 percent to about 1 percent castor oil.
16. The dosage form of claim 15 wherein the hydroxypropylmethylcellulose has a viscosity of about 5000 cps in an aqueous solution containing 2 weight percent hydroxypropylmethylcellulose.
17. The dosage form of claim 11 wherein the subcoating is comprised of, based upon the total dry weight of the subcoating,
a) from about 20 percent to about 50 percent hydroxypropylmethylcellulose;
b) from about 45 percent to about 75 percent maltodextrin;
c) from about 1 percent to about 10 percent PEG 400.
18. The coated dosage form of claim 11, wherein the coating is comprised of, based upon the total dry weight of the subcoating,
a) from about 25 percent to about 40 percent hydroxyethylcellulose;
b) from about 50 percent to about 70 percent maltodextrin;
c) from about 5 percent to about 10 percent PEG 400.
19. A tablet coated with the film forming composition according to claim 2.
20. The coated dosage form of claim 11, further comprising an effective amount of a pharmaceutical active ingredient, wherein said dosage form meets USP dissolution requirements for immediate release forms of said pharmaceutical active ingredient.
21. An aqueous dispersion of the composition of claim 2 for the manufacture of dip-coated tablets.
22. The aqueous dispersion of claim 21 comprised of, based upon the total weight of the aqueous dispersion,
a) from about 10 percent to about 14 percent of hydroxypropylmethylcellulose; and
b) from about 0.1 percent to about 0.14 percent of xanthan gum.
23. The composition of claim 1 which is substantially free of gelatin.
24. Use of the aqueous dispersion of claim 21 for the manufacture of dip coated tablets.
25. A pharmaceutical dosage form comprising a core and a coating, said coating substantially covering said core and having a surface gloss of at least 150; wherein said coating comprises the composition of claim 1.
26. The composition of claim 2 further comprising a weight enhancer selected from the group consisting of simethicone, polysorbate 80, and mixtures thereof.
27. A simulated capsule-like medicament comprising:
a. a core having a first end and a second end,
b. a first coating layer having a first color provided on said first end of said core;
c. a second coating layer having a second color on said second end of said core, said first color is different from said second color; wherein at least one of said first coating layer and second coating layer comprises the composition of claim 1.
28. The medicament of claim 27 wherein at least one of said first coating layer and said second coating layer is comprised of a coating composition, said coating composition comprised of, based upon the total dry weight of the coating composition,
a) from about 95 percent to less than about 100 percent of hydroxypropylmethyl cellulose; and
b) from about 0.5 percent to about 5 percent of xanthan gum.
29. The medicament of claim 28 further comprising a subcoating layer substantially covering said core, said subcoating layer provided between said core and said first coating layer and said second coating layer.
30. The medicament of claim 29 wherein the subcoating comprises materials selected from the group consisting of cellulose ethers, plasticizers, polycarbohydrates, pigments, opacifiers, and mixtures thereof.
31. A water soluble composition for dip-coating a substrate comprised of:
a) hydroxypropylmethyl cellulose; and
b) castor oil,
wherein the composition possesses a surface gloss of at least 150 when applied via dip coating to a substrate.
32. A pharmaceutical dosage form comprising a core and a coating, said coating substantially covering said core; wherein said coating comprises the composition of claim 31.
33. Use of the composition of claim 31 for the manufacture of dip coated tablets.
34. A water soluble composition for dip-coating a substrate comprised of:
a) hydroxypropylmethyl cellulose; and
b) maltodextrin,
wherein the composition possesses a surface gloss of at least 150 when applied via dip coating to a substrate.
35. The composition of claim 34 further comprising polyethylene glycol.
36. A pharmaceutical dosage form comprising a core and a coating, said coating substantially covering said core; wherein said coating comprises the composition of claim 34.
37. Use of the composition of claim 34 for the manufacture of dip coated tablets.
38. The water soluble composition of claim 1, wherein the composition is comprised of, based upon the total dry weight of the composition:
a) greater than about 95 percent and less than about 99.5 percent of hydroxypropylmethyl cellulose; and
b) greater than about 0.5 percent and less than about 5 percent of carrageenan,
wherein the composition possesses a surface gloss of at least 150 when applied via dip coating to a substrate.
39. A pharmaceutical dosage form comprising a core and a coating,
said coating substantially covering said core; wherein said coating comprises the composition of claim 38.
40. Use of the composition of claim 38 for the manufacture of dip coated tablets.

What is claimed is:

1. A method for cloning or subcloning one or more desired nucleic acid molecules comprising
(a) combining in vitro or in vivo
(i) one or more Insert Donor molecules comprising one or more desired nucleic acid segments flanked by at least two recombination sites, wherein said recombination sites do not substantially recombine with each other;
(ii) one or more Vector Donor molecules comprising at least two recombination sites, wherein said recombination sites do not substantially recombine with each other; and
(iii) one or more site-specific recombination proteins;

(b) incubating said combination under conditions sufficient to transfer one or more of said desired segments into one or more of said Vector Donor molecules, thereby producing one or more desired Product nucleic acid molecules;
(c) combining in vitro or in vivo
(i) one or more of said Product molecules comprising said desired segments flanked by two or more recombination sites, wherein said recombination sites do not substantially recombine with each other;
(ii) one or more different Vector Donor molecules comprising two or more recombination sites, wherein said recombination sites do not substantially recombine with each other; and
(iii) one or more site-specific recombination proteins; and

(d) incubating said combination under conditions sufficient to transfer one or more of said desired segments into one or more different Venter Donor molecules, thereby producing one or more different Product molecules.
2. The method of claim 1, further comprising incubating said different Product molecules with one or more different Vector Donor molecules under conditions sufficient to transfer one or more of said desired segments into said different Vector Donor molecules.
3. A method for cloning or subcloning desired nucleic acid molecules comprising
a) combining in vitro or in vivo
i) one or more Insert Donor molecules comprising one or more nucleic acid segments flanked by two or more recombination sites, wherein said recombination sites do not substantially recombine with each other;
ii) two or more different Vector Donor molecules comprising two or more recombination sites, wherein said recombination sites do not substantially recombine with each other; and
iii) one or more site specific recombination proteins; and

b) incubating said combination under conditions sufficient to transfer one or more of said desired segments into said different Vector Donor molecules, thereby producing two or more different Product molecules.
4. The method of claim 1 or claim 3, wherein said Insert Donor molecules are derived from genomic DNA.
5. The method of claim 1 or claim 3, wherein said Insert Donor molecules are derived from cDNA.
6. The method of claim 1 or claim 3, wherein said Insert Donor molecules are produced by chemical synthesis.
7. The method of claim 1 or claim 3, wherein said Vector Donor molecules comprise at least one Selectable marker.
8. The method of claim 7, wherein the Selectable marker comprises at least one DNA segment selected from the group consisting of:
(a) a DNA segment that encodes a product that provides resistance in a recipient cell against otherwise toxic compounds;
(b) a DNA segment that encodes a product that is otherwise lacking in a recipient cell;
(c) a DNA segment that encodes a product that suppresses the activity of a gene product in a recipient cell;
(d) a DNA segment that encodes a product that can be identified;
(e) a DNA segment that encodes a product that inhibits a cell function in a recipient cell;
(f) a DNA segment that inhibits the activity of any of the DNA segments of (a)-(e) above;
(g) a DNA segment that binds a product that modifies a substrate;
(h) a DNA segment that encodes a specific nucleotide recognition sequence which can be recognized by a protein, an RNA, DNA or chemical.
(i) a DNA segment that, when deleted, directly or indirectly confers sensitivity to cell killing by particular compounds within a recipient cell;
(j) a DNA segment that encodes a product that is toxic in a recipient cell; and
(k) a DNA segment that can be used to isolate or identify a desired molecule.
9. The method of claim 8, wherein said Selectable marker comprises at least one marker selected from the group consisting of an antibiotic resistance gene, a tRNA gene, an auxotrophic marker, a toxic gene, a phenotypic marker, an anti sense oligonucleotide, a restriction endonuclease, a restriction endonuclease cleavage site, an enzyme cleavage site, a protein binding site, and a sequence complementary to a PCR primer sequence.
10. The method of claim 1 or claim 3, wherein said Vector Donor molecules comprise prokaryotic andor eukaryotic vectors.
11. The method of claim 10, wherein said eukaryotic vectors comprise vectors which propagate andor replicate in yeast cells, plant cells, fish cells, eukaryotic cells, mammalian cells, andor insect cells.
12. The method of claim 10, wherein said prokaryotic vectors comprise vectors which propagate andor replicate in gram negative or gram positive bacteria.
13. The method of claim 12, wherein said prokaryotic vectors comprise vectors which propagate andor replicate in bacteria of the genus Escherichia, Salmonella, Bacillus, Streptomyces andor Pseudemonas.
14. The method of claim 13, wherein said prokaryotic vector comprises a vector which propagates andor replicates in E. coli.
15. The method of claim 10, wherein said Vector Donor molecules are selected from the group consisting of cloning vectors, sequencing vectors, expression vectors, fusion vectors, 2-hybrid vectors, reverse 2-hybrid vectors or derivatives or variants thereof.
16. The method of claim 10, wherein said eukaryotic vectors are selected from the group consisting of pFastBac, pFastBac HT, pFastBac DUAL, pSFV, pTet-Splice, pEUK-C1, pPUR, pMAM, pMAMneo, pBI101, pBI121, pDR2, pCMVEBNA, YACneo, pSVK3, pSVL, pMSG, pCH110, pKK232-8, p3SS, pXT1, pSG5, pPbac, pMbac, pMC1neo, and pOG44, pYES2, pAC360, pBlueBacHis, pVL1392, pBlueBacEIII, pCDM8, pcDNA1, pZeoSV, pcDNA3 pREP4, pCEP4, and pEBVHis or derivatives or variants thereof.
17. The method of claim 10 , wherein said prokaryotic vectors are selected from the group consisting of pcDNA II, pSL301, pSE280, pSE380, pSE420, pTrcHis, pRSET, pGEMEX-1, pGEMEX-2, pET, pTrc99A, pKK223-3, pGEX, pEZZ18, pRIT2T, pMC1871, pKK233-2, pKK388-1, and pProEx-HT or derivatives or variants thereof.
18. The method of claim 15, wherein said 2-hybrid and reverse 2-hybrid vectors are selected from the group consisting of pPC86, pDBLeu, pDBTrp, pPC97, p2.5, pGAD1-3, pGAD10, pACt, pACT2, pGADGL, pGADGH, pAS2-1, pGAD424, pGBT8, pGBT9, pGAD-GAL4, pLexA, pBD-GAL4, pHISi, pHISi-1, placZi, pB42AD, pDG202, pJK202, pJG4-5, pNLexA, and pYESTrp or derivatives or variants thereof.
19. The method of claim 1 or claim 3, wherein said Insert Donor molecules comprise a vector.
20. The method of claim 1 or claim 3, wherein said Insert Donor molecules comprise a DNA segment produced by amplification.
21. The method of claim 20, wherein said amplification is PCR.
22. The method of claim 21, wherein said Insert Donor is linear.
23. The method of claim 22, wherein said Insert Donor comprises at least one recombination site at or near one or both termini of said linear molecule.
24. The method of claim 1 or claim 3, wherein said recombination sites are selected from the group consisting of loxP, attB, attP, attL, and attR.
25. The method of claim 1 or claim 3, wherein said recombination proteins are selected from the group consisting of Int, Cre, Flp, Res.
26. A method for preparing a nucleic acid molecule comprising two or more recombination sites or portions thereof comprising
(a) mixing a nucleic acid template with a polypeptide having polymerase activity and one or more primers comprising one or more recombination sites or portions thereof; and
(b) incubating said mixture under conditions sufficient to synthesize a nucleic acid molecule which is complementary to all or a portion of said template and which comprises one or more recombination sites or portions thereof.
27. The method of claim 26, further comprising incubating said synthesized molecule in the presence of one or more primers comprising one or more recombination sites or portions thereof under conditions sufficient to synthesize a second nucleic acid molecule complementary to all or a portion to said first nucleic acid molecule, thereby producing a double stranded nucleic acid molecule comprising two or more recombination sites or portions thereof.
28. The method of claim 27, wherein said recombination sites or portions thereof are located at or near one or more termini of said synthesized double stranded nucleic acid molecule.
29. The method of claim 27, wherein said template is RNA or DNA.
30. The method of claim 29, wherein said RNA is an mRNA or a polyA RNA molecule.
31. The method of claim 27, wherein said polypeptide is selected from the group consisting of a reverse transcriptase or DNA polymerase.
32. The method of claim 31, wherein said DNA polymerase is a thermostable DNA polymerase.
33. The method of claim 32, wherein said thermostable DNA polymerase is selected from the group consisting of Thermus thermophilus (Tth) DNA polymerase, Thermus aquaticus (Taq) DNA polymerase, Thermatoga neopolitana (Tne) DNA polymerase, Thermatoga maritima (Tma) DNA polymerase, Thermococcus litoralis (Tli or VENT) DNA polymerase, Pyrococcus furiosus (Pfu or DEEPVENT) DNA polymerase, Pyrococcus woosii (Pwo) DNA polymerase, Bacillus sterothermophilus (Bst) DNA polymerase, Sulfolobus acidocaldarius (Sac) DNA polymerase, Thermoplasma acidophilum (Tac) DNA polymerase, Thermus flavus (TflTub) DNA polymerase, Thermus ruber (Tru) DNA polymerase, Thermus brockianus (DYNAZYME) DNA polymerase, Methanobacterium thermoautotrophicum (Mth) DNA polymerase, and mutants, variants and derivatives thereof.
34. The method of claim 27, further comprising amplifying said first and second nucleic acid molecules.
35. The method of claim 34, wherein said amplification is accomplished by a method comprising
(a) contacting said first nucleic acid molecule with a first primer which is complementary to a portion of said first nucleic acid molecule, and a second nucleic acid molecule with a second primer which is complementary to a portion of said second nucleic acid molecule with a polypeptide having polymerase activity;
(b) incubating said mixture under conditions sufficient to form a third nucleic acid molecule complementary to all or a portion of said first nucleic acid molecule and a fourth nucleic acid molecule complementary to all or a portion of said second nucleic acid molecule;
(c) denaturing said first and third and said second and fourth nucleic acid molecules; and
(d) repeating steps (a) through (c) one or more times, wherein said first primer andor said second primer comprise one or more recombination sites or portions thereof.
36. A method for amplifying a nucleic acid molecule comprising
(a) contacting a first nucleic acid molecule with a first primer which is complementary to a portion of said first nucleic acid molecule, and a second nucleic acid molecule with a second primer which is complementary to a portion of said second nucleic acid molecule with a polypeptide having polymerase activity;
(b) incubating said mixture under conditions sufficient to form a third nucleic acid molecule complementary to all or a portion of said first nucleic acid molecule and a fourth nucleic acid molecule complementary to all or portion of all or said portion of said second nucleic acid molecule;
(c) denaturing said first and third and said second and fourth nucleic acid molecule; and
(d) repeating steps (a) through (c) one or more times, wherein said first primer andor second primer comprise one or more recombination sites or portions thereof.
37. A method for producing one or more cDNA molecules or a population of cDNA molecules comprising
(a) mixing an RNA template or population of RNA templates with a reverse transcriptase and one or more primers wherein said primers comprise one or more recombination sites or portions thereof; and
(b) incubating said mixture under conditions sufficient to make a first DNA molecule complementary to all or a portion of said template, thereby forming a first DNA molecule comprising one or more recombination sites or portions thereof.
38. The method of claim 37, further comprising incubating said first DNA molecule with one or more primers which comprise one or more recombination sites or portions thereof under conditions sufficient to make a second DNA molecule complementary to all or a portion of said first DNA molecule, thereby producing a double stranded DNA molecule which comprises one or more recombination sites or portions thereof.
39. The method of claim 38, wherein said double stranded DNA molecule is linear.
40. The method of claim 39, wherein said double stranded DNA molecule comprises one or more recombination sites or portions thereof at or near one or both termini of said double stranded DNA molecule.
41. A method for synthesizing one or more nucleic acid molecules comprising one or more recombination sites, said method comprising:
(a) obtaining one or more linear nucleic acid molecules; and
(b) contacting said molecules with one or more adapters which comprise one or more recombination sites or portions thereof under conditions sufficient to add one or more of said adapters to one or more termini of said linear nucleic acid molecule.
42. The method of claim 41, wherein said linear nucleic acid molecules are derived from genomic DNA.
43. The method of claim 41, wherein said linear nucleic acid molecules are derived from cDNA.
44. The method of claim 41, wherein said linear nucleic acid molecules are produced by mechanical or enzymatic techniques.
45. The method of claim 41, wherein said linear nucleic acid molecules are produced by digesting one or more nucleic acid molecules with one or more restriction endonucleases.
46. A method for adding one or more recombination sites or portions thereof to one or more nucleic acid molecules, said method comprising:
(a) contacting one or more nucleic acid molecules with one or more integration sequences which comprise one or more recombination sites or portions thereof; and
(b) incubating said mixture under conditions sufficient to incorporate said integration sequences into said nucleic acid molecules.
47. The method of claim 46, wherein said integration sequences are selected from the group consisting of transposons, integrating viruses, integrating elements, integrons and recombination sequences.
48. The method of claim 47, wherein said integration sequence is added to genomic DNA.
49. A product produced by the process of any one of claims 1, 3, 27, 37, 41, and 46.
50. The method of claim 1 or claim 3, wherein said segment is produced by chemical synthesis.
51. The method of claim 47, wherein said integration sequence is added to a vector.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An apparatus, comprising
a passive radio frequency identification (RFID) tag circuit comprising an oscillator circuit having a component sensitive to an environmental factor, wherein a frequency of a signal output by the oscillator circuit is indicative of a value of the environmental factor.
2. The apparatus of claim 1, wherein a transmission from the RFID tag circuit comprises:
a first section modulated with an identification code of the RFID tag circuit; and
a second section modulated with the signal output by the oscillator circuit.
3. The apparatus of claim 2, wherein the identification code is encoded as a digital value and the output of the oscillator circuit is encoded as an oscillating analog signal.
4. The apparatus of claim 2, wherein the second section includes a first portion indicating a value of the environmental factor and a second portion indicating a value of a second environmental factor.
5. The apparatus of claim 1, wherein the component is a thermistor.
6. The apparatus of claim 1, wherein the component is a capacitive hygrometer.
7. The apparatus of claim 1, further comprising a dipole antenna coupled to the RFID tag circuit.
8. The apparatus of claim 1, wherein at least a part of the oscillator circuit is in a same integrated circuit as the RFID tag circuit.
9. The apparatus of claim 1, where all of the oscillator circuit is in a same integrated circuit as the RFID tag circuit.
10. An apparatus, comprising
a radio frequency identification (RFID) reader device configured to:
receive a wireless response from a passive RFID tag;
demodulate a first section of the response to determine an identification code for the RFID tag; and
demodulate a second section of the response to determine a first value of a first environmental factor sensed by the RFID tag, wherein the first value is based on at least one parameter selected from a list consisting of a frequency of an oscillating signal derived from the demodulated second section and a pulse width determination of the oscillating signal derived from the demodulated second section.
11. The apparatus of claim 10, wherein the RFID reader device is further to demodulate a third section of the response to determine a second value of a second environmental factor sensed by the RFID tag, wherein the second value is based on a signal derived from the demodulated third section.
12. The apparatus of claim 10, wherein the RFID reader device further comprises an element selected from a list consisting of a dipole antenna and a battery.
13. A method, comprising:
sensing an environmental parameter;
producing an oscillating signal whose frequency is indicative of a value of the sensed environmental parameter; and
transmitting a wireless signal from a radio frequency identification (RFID) tag, a first section of the signal modulated with an identification code for the RFID tag and a second section of the signal modulated with the oscillating signal;
wherein said producing takes place within the RFID tag.
14. The method of claim 13, further comprising harvesting electrical power from a received wireless signal to power circuitry of the RFID tag.
15. The method of claim 13, wherein said modulating the first section of the signal comprises modulating the signal with a digital waveform and said modulating the second section of the signal comprises modulating the signal with an oscillating waveform.
16. The method of claim 13, wherein said sensing an environmental parameter comprises at least one of sensing temperature and sensing humidity.
17. A method, comprising:
receiving a wireless response from a radio frequency identification (RFID) tag, the response including a first section modulated with an identification code of the RFID tag and a second section modulating with an oscillating signal whose frequency indicates a value of an environmental factor.
18. The method of claim 17, further comprising demodulating the second section to reproduce the oscillating signal.
19. The method of claim 18, wherein said demodulating the second section comprises digitizing the oscillating signal and performing a mathematical operation on the digitized oscillating signal.
20. The method of claim 19, further comprising determining the value of the environmental factor by determining a parameter selected from a list consisting of: 1) the frequency of the signal, and 2) pulse width determination of the signal.
21. An article comprising
a tangible machine-readable medium that contains instructions, which when executed by one or more processors result in performing operations comprising:
receiving a wireless response from a radio frequency identification (RFID) tag, the response including a first section modulated with an identification code of the RFID tag and a second section modulated with an oscillating signal whose frequency indicates a value of an environmental factor.
22. The article of claim 21, wherein the operations further comprise digitizing a demodulated version of the oscillating signal and performing a mathematical operation on the digitized signal to derive the frequency.
23. The article of claim 22, wherein the operation of performing a mathematical operation comprises performing a Fourier Transform.
24. The article of claim 21, wherein the operations further comprise converting the derived frequency to the value of the environment factor.