Author: claimsbot

1. An image generating system comprising:
means adapted for acquiring document image data representative of a document;
a memory for storing the document image data;
means adapted to establish a page length corresponding to a number of pages associated with each document of a specific set of documents, wherein each document contains the same number of pages;
an inputting means adapted to store page data representative of a pre-set number of pages shared in common by each document of the specific set of documents; and
an image generating means adapted to generate, in the memory, image data of each document of the set of documents; and
means adapted for generating separator data so as to identify the image data into distinct portions based on stored page data.
2. The image generating system of claim 1 wherein the inputting means includes means adapted for manually inputting the pre-set page length including at least one of a keyboard, a user interface on the image generating device, and via a network application or web browser.
3. The image generating system of claim 1 further comprising means adapted to collect and stack the set of documents with the specified number of pages.
4. The image generating system of claim 1 further comprising means adapted for selecting the desired parameters for generating the image data.
5. The image generating system of claim 4 further comprising a display for generating an image of the document image data.
6. The image generating system of claim 1 wherein the image generating means includes optical character recognition systems, image generating devices, and other multif unction peripherals.
7. The image generating system of claim 1 wherein the image generating means generates the set of documents into TIFF files.
8. The image generating system of claim 1 further comprising:
means adapted to change the pre-set page length for each document to a new established page length; and
means adapted to generate separator data so as to identify the image data into distinct portions having the new pre-set page length.
9. A method for generating images comprising the steps of:
acquiring image data representative of a document;
storing the document image data in memory;
establishing a page length corresponding to a number of pages associated with each document of a specific set of documents, wherein each document contains the same number of pages;
inputting the page length for each document of the specific set of documents into an image generating apparatus as a pre-set value;
generating, in the memory, image data of each document of the set of documents; and
generating separator data so as to identify the image data into distinct portions based on the pre-set page length.
10. The method of claim 9 wherein the page length of the specific set of documents is manually input into the image generating apparatus via a keyboard, a user interface on the image generating device, and via a network application or web browser.
11. The method of claim 9 further comprising collecting and stacking the set of documents with the specified number of pages.
12. The method of claim 9 further comprising selecting the desired parameters for generating the image data.
13. The method of claim 12 further comprising a display for generating an image of the document image data.
14. The method of claim 9 wherein the image generating apparatus includes optical character recognition systems, image generating devices, and other multifunction peripherals.
15. The method of claim 9 wherein the image generating apparatus generates the set of documents into TIFF files.
16. The method of claim 9 further comprising:
changing the pre-set page length for each document to a new established page length; and
generating separator data as to identify the image data into distinct portions based on the new pre-set page length.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A vector comprising a nucleic acid sequence encoding a bonding partner, wherein the nucleic acid sequence encoding the bonding partner comprises a nucleic acid sequence encoding a carrier polypeptide and a nucleic acid sequence encoding a target sequence, wherein the nucleic acid sequence encoding the target sequence is heterologous to the nucleic acid sequence encoding the carrier polypeptide, and the target sequence comprises at least 2 cysteines and has the sequence of Cys-Cys-Rn, whether R is any amino acid and n is an integer from 1-100, and wherein when n\u22672, each R is selected independently from every other R in the sequence, but wherein the target sequence cannot be Cys-Cys-Xaa-Xaa-Cys-Cys (SEQ. ID NO: 1) wherein Xaa can be any amino acid, and wherein the target sequence reacts with a biarsenical molecule having the formula:
and tautomers, anhydrides, and salts thereof;
wherein:
each X1 or X2 is independently Cl, Br, I, ORa, or SRa, or
X1 and X2 together with the arsenic atom form a ring having the formula:
Ra is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Z is 1,2-ethanediyl, 1,2-propanediyl, 2,3-butanediyl, 1,3-propanediyl, 1,2 benzenediyl, 4-methyl-1,2-benzenediyl, 1,2-cyclopentanediyl, 1,2-cyclohexanediyl, 3-hydroxy-1,2-propanediyl, 3-sulfo-1,2-propanediyl, or 1,2-bis(carboxy)-1,2-ethanediyl;
Y1 and Y2, are each independently H or CH3, or
Y1 and Y2 together form a ring such that the biarsenical molecule has the formula
wherein:
M is O, S, CH2, C(CH3)2, or NH;
R1 and R2 are each independently ORa, OAc, NRaRb, or H;
R3 and R4 are each independently H, F, Cl, Br, I, ORa, or Ra; or
R1 together with R3, or R2 together with R4, or both, form a ring in which

(i) one of R1 or R3 is C2-C3 alkyl and the other is NRa and
(ii) one of R2 and R4 is C2-C3 alkyl and the other is NRa;
Rb is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Q is CRaRb, CRaORb, C\u2550O, or a spirolactone having the formula:
wherein the spiro linkage is formed at C1
2. The vector of claim 1, wherein the target sequence is Cys-Cys-X1-X1-X2-X1-X3-X1-X1-Cys-X1-Cys-X2 (SEQ. ID NO: 3), wherein:
X1 is an amino acid having a non-polar side chain,
X2 is an amino acid having a basic side chain, and
X3 is an amino acid having a non-ionic polar side chain.
3. The vector of claim 2, wherein X1 is glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine, or tryptophan.
4. The vector of claim 2, wherein X2 is lysine, arginine, or histidine.
5. The vector of claim 2, wherein X3 is asparagine, glutamine, serine, or threonine.
6. The vector of claim 1, wherein the target sequence is Cys-Cys-gly-gly-lys-gly-asn-gly-gly-Cys-gly-Cys-his (SEQ. ID NO: 50).
7. The vector of claim 1, wherein the nucleic acid sequence encoding the target sequence is attached at the 5\u2032-end of the nucleic acid sequence encoding the carrier polypeptide.
8. The vector of claim 1, wherein the nucleic acid sequence encoding the target sequence is attached at the 3\u2032-end of the nucleic acid sequence encoding the carrier polypeptide.
9. The vector of claim 1, wherein the biarsenical molecule has the following structure:
10. A method of labeling a carrier molecule, comprising:
a) providing a bonding partner comprising the carrier molecule and a target sequence, and
b) contacting the bonding partner with a biarsenical molecule under conditions wherein the biarsenical molecule reacts with the target sequence, wherein the target sequence comprises at least 2 cysteines and has the sequence Cys-Cys-Rn, wherein R is any amino acid and n is an integer from 1-100, and wherein when n\u22672, each R is selected independently from every other R in the sequence, but wherein the target sequence cannot be Cys-Cys-Xaa-Xaa-Cys-Cys (SEQ. ID NO: 1) wherein Xaa can be any amino acid, and wherein the biarsenical molecule has the formula:
and tautomers, anhydrides, and salts thereof;
wherein:
each X1 or X2 is independently Cl, Br, I, ORa, or SRa, or
X1 and X2 together with the arsenic atom form a ring having the formula:
Ra is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Z is 1,2-ethanediyl, 1,2-propanediyl, 2,3-butanediyl, 1,3-propanediyl, 1,2 benzenediyl, 4-methyl-1,2-benzenediyl, 1,2-cyclopentanediyl, 1,2-cyclohexanediyl, 3-hydroxy-1,2-propanediyl, 3-sulfo-1,2-propanediyl, or 1,2-bis(carboxy)-1,2-ethanediyl;
Y1 and Y2 are each independently H or CH3, or
Y1 and Y2 together form a ring such that the biarsenical molecule has the formula
wherein:
M is O, S, CH2, C(CH3)2, or NH;
R1 and R2 are each independently ORa, OAc, NRaRb, or H;
R3 and R4 are each independently H, F, Cl, Br, I, ORa, or Ra; or
R1 together with R3, or R2 together with R4, or both, form a ring in which

(i) one of R1 or R3 is C2-C3 alkyl and the other is NRa and
(ii) one of R2 and R4 is C2-C3 alkyl and the other is NRa;
Rb is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Q is CRaRb, CRaORb, C\u2550O, or a spirolactone having the formula:
wherein the spiro linkage is formed at C1.
11. The method of claim 10, wherein the biarsenical molecule generates a detectable signal.
12. The method of claim 11, further comprising monitoring the detectable signal.
13. The method of claim 11, wherein the signal is a fluorescent signal.
14. The method of claim 10, wherein the biarsenical molecule is coupled to a solid phase.
15. The method of claim 10, wherein the target sequence is coupled to a solid phase.
16. The method of claim 10, wherein the carrier molecule is a polypeptide.
17. The method of claim 16, wherein the polypeptide is an antibody or an enzyme.
18. The method of claim 10, wherein the target sequence is Cys-Cys-X1-X1-X2-X1-X3-X1-X1-Cys-X1-Cys-X2 (SEQ. ID NO: 3), wherein:
X1 is an amino acid having a non-polar side chain,
X2 is an amino acid having a basic side chain, and
X3 is an amino acid having a non-ionic polar side chain.
19. The method of claim 18, wherein X1 is glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine, or tryptophan.
20. The method of claim 18, wherein X2 is lysine, arginine, or histidine.
21. The method of claim 18, wherein X3 is asparagine, glutamine, serine, or threonine.
22. The method of claim 10, wherein the target sequence is Cys-Cys-gly-gly-lys-gly-asn-gly-gly-Cys-gly-Cys-his (SEQ. ID NO: 50).
23. A kit comprising:
a) a biarsenical molecule having the structure:
and tautomers, anhydrides, and salts thereof;
wherein:
each X1 or X2 is independently Cl, Br, I, ORa, or SRa, or
X1 and X2 together with the arsenic atom form a ring having the formula:
Ra is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Z is 1,2-ethanediyl, 1,2-propanediyl, 2,3-butanediyl, 1,3-propanediyl, 1,2 benzenediyl, 4-methyl-1,2-benzenediyl, 1,2-cyclopentanediyl, 1,2-cyclohexanediyl, 3-hydroxy-1,2-propanediyl, 3-sulfo-1,2-propanediyl, or 1,2-bis(carboxy)-1,2-ethanediyl;
Y1 and Y2 are each independently H or CH3, or
Y1 and Y2 together form a ring such that the biarsenical molecule has the formula
wherein:
M is O, S, CH2, C(CH3)2, or NH;
R1 and R2 are each independently ORa, OAc, NRaRb, or H;
R3 and R4 are each independently H, F, Cl, Br, I, ORa, or Ra; or
R1 together with R3, or R2 together with R4, or both, form a ring in which

(i) one of R1 or R3 is C2-C3 alkyl and the other is NRa and
(ii) one of R2 and R4 is C2-C3 alkyl and the other is NRa;
Rb is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Q is CRaRb, CRaORb, C\u2550O, or a spirolactone having the formula:
wherein the spiro linkage is formed at C1; and
b) a bonding partner comprising a target sequence, wherein the target sequence comprises at least 2 cysteines and has the sequence Cys-Cys-Rn, wherein R is any amino acid and n is an integer from 1-100, and wherein when n\u22672, each R is selected independently from every other R in the sequence, but wherein the target sequence cannot be Cys-Cys-Xaa-Xaa-Cys-Cys (SEQ. ID NO: 1) wherein Xaa can be any amino acid, and wherein the target sequence reacts with the biarsenical molecule.
24. The kit of claim 23, wherein the target sequence is Cys-Cys-X1-X1-X2-X1-X3-X1-X1-Cys-X1-Cys-X2 (SEQ. ID NO: 3), wherein:
X1 is an amino acid having a non-polar side chain,
X2 is an amino acid having a basic side chain, and
X3 is an amino acid having a non-ionic polar side chain.
25. The kit of claim 24, wherein X1 is glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine, or tryptophan.
26. The kit of claim 24, wherein X2 is lysine, arginine, or histidine.
27. The kit of claim 24, wherein X3 is asparagine, glutamine, serine, or threonine.
28. The kit of claim 23, wherein the target sequence is Cys-Cys-gly-gly-lys-gly-asn-gly-gly-Cys-gly-Cys-his (SEQ. ID NO: 50).
29. A method of purifying a bonding partner, comprising:
a) providing a bonding partner comprising a target sequence, comprises at least 2 cysteines and has the sequence of Cys-Cys-Rn, whether R is any amino acid and n is an integer from 1-100, and wherein when n\u22672, each R is selected independently from every other R in the sequence, but wherein the target sequence cannot be Cys-Cys-Xaa-Xaa-Cys-Cys (SEQ. ID NO: 1) wherein Xaa can be any amino acid, and wherein the target sequence reacts with a biarsenical molecule having the structure:
and tautomers, anhydrides, and salts thereof;
wherein:
each X1 or X2 is independently Cl, Br, I, ORa, or SRa, or
X1 and X2 together with the arsenic atom form a ring having the formula:
Ra is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Z is 1,2-ethanediyl, 1,2-propanediyl, 2,3-butanediyl, 1,3-propanediyl, 1,2 benzenediyl, 4-methyl-1,2-benzenediyl, 1,2-cyclopentanediyl, 1,2-cyclohexanediyl, 3-hydroxy-1,2-propanediyl, 3-sulfo-1,2-propanediyl, or 1,2-bis(carboxy)-1,2-ethanediyl;
Y1 and Y2 are each independently H or CH3, or
Y1 and Y2 together form a ring such that the biarsenical molecule has the formula
wherein:
M is O, S, CH2, C(CH3)2, or NH;
R1 and R2 are each independently ORa, OAc, NRaRb, or H;
R3 and R4 are each independently H, F, Cl, Br, I, ORa, or Ra; or
R1 together with R3, or R2 together with R4, or both, form a ring in which

(i) one of R1 or R3 is C2-C3 alkyl and the other is NRa and
(ii) one of R2 and R4 is C2-C3 alkyl and the other is NRa;
Rb is H, C1-C4 alkyl, CH2CH2OH, CH2COOH, or CN;
Q is CRaRb, CRaORb, C\u2550O, or a spirolactone having the formula:
wherein the spiro linkage is formed at C1;
d) contacting the bonding partner with the biarsenical molecule, wherein the biarsenical molecule is coupled to a solid phase,
e) eluting the bonding partner from the biarsenical molecule by contacting the biarsenical molecule with a dithiol.
30. The method of claim 29, wherein the carrier molecule is a protein.
31. The method of claim 30, wherein the protein is an antibody or an enzyme.
32. The method of claim 29, wherein the biarsenical compound is membrane permeable.
33. The method of claim 29, wherein the dithiol is selected from the group consisting of 1,2-benzendithiol, 1,2-cyclohexanedithiol and 1,2-ethanedithiol.
34. An isolated polypeptide comprising a sequence Cys-Cys-X1-X1-X2-X1-X3-X1-X1-Cys-X1-Cys-X2 (SEQ. ID NO: 3), wherein:
X1 is an amino acid having a non-polar side chain,
X2 is an amino acid having a basic side chain, and
X3 is an amino acid having a non-ionic polar side chain.
35. The polypeptide of claim 34, wherein X1 is glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine, or tryptophan.
36. The polypeptide of claim 34, wherein X2 is lysine, arginine, or histidine.
37. The polypeptide of claim 34, wherein X3 is asparagine, glutamine, serine, or threonine.
38. An isolated polypeptide comprising a sequence Cys-Cys-Gly-Gly-Lys-Gly-Asn-Gly-Gly-Cys-Gly-Cys-His (SEQ. ID. NO: 50).
39. An isolated nucleic acid molecule encoding a polypeptide having a sequence Cys-Cys-X1-X1-X2-X1-X3-X1-X1-Cys-X1-Cys-X2 (SEQ. ID NO: 3), wherein:
X1 is an amino acid having a non-polar side chain,
X2 is an amino acid having a basic side chain, and
X3 is an amino acid having a non-ionic polar side chain.
40. The isolated nucleic acid molecule of claim 39, wherein X1 is glycine, alanine, valine, leucine, isoleucine, methionine, proline, phenylalanine, or tryptophan.
41. The isolated nucleic acid molecule of claim 39, wherein X2 is lysine, arginine, or histidine.
42. The isolated nucleic acid molecule of claim 39, wherein X3 is asparagine, glutamine, serine, or threonine.
43. An isolated nucleic acid molecule encoding a polypeptide having a sequence Cys-Cys-Gly-Gly-Lys-Gly-Asn-Gly-Gly-Cys-Gly-Cys-His (SEQ. ID. NO: 50).

1. A sensor element for determining a concentration of at least one gas component in a gas mixture, comprising:
at least one first electrode exposed to the at least one gas component to be analyzed;
a second arrangement;
at least one reference electrode electrically interconnected by a first solid electrolyte to the at least one first electrode, wherein the second arrangement is configured to measure an electric current at least one of continuously and non-continuously between the at least one first electrode and one of the at least one reference electrode and a counterelectrode, in response to at least one of the applying and the setting an electric voltage between the at least one first electrode and the at least one reference electrode due to a chemical reaction proceeding at the at least one first electrode, the concentration of the at least one gas component in the gas mixture being determinable from the electric current; and
a first arrangement configured to at least one of apply and set the electric voltage between the at least one first electrode and the at least one reference electrode only during recurring time intervals;
wherein the second arrangement is configured to measure the electric current between the at least one first electrode and one of the at least one reference electrode and the counterelectrode, at least intermittently within the recurring time intervals.
2. The sensor element of claim 1, further comprising:
at least one reference gas channel, wherein the at least one reference gas is applied to the at least one reference electrode via the at least one reference gas channel.
3. The sensor element of claim 1, wherein the sensor element includes a first chamber, to which the gas mixture can be applied, that is provided with a second electrode which is connected to the counterelectrode via a second solid electrolyte, and a second chamber, downstream of the first chamber and connected to the first chamber in a gas-permeable manner, in which the at least one first electrode is positioned.
4. The sensor element of claim 3, wherein the first chamber together with the second electrode and the counterelectrode is a pump cell for electrochemically pumping off oxygen from the gas mixture.
5. The sensor element of claim 3, further comprising:
a first diffusion barrier positioned in the first chamber; and
a second diffusion barrier positioned in the second chamber;
wherein the gas mixture at least one of accesses the first chamber via the first diffusion barrier and accesses the second chamber via the second gas diffusion barrier.
6. The sensor element of claim 1, wherein the first arrangement includes an electric circuit and a voltage source for generating at least one of a specific electric voltage and a predefinable electric voltage that is constant during the recurring time intervals.
7. The sensor element of claim 1, wherein the first arrangement is configured to select a length of the recurring time intervals so that the electric current, which is measured as a function of time during the recurring time intervals, between the at least one first electrode and the at least one reference electrode is greater than a limiting current, measured at a constant electric voltage during a corresponding time.
8. The sensor element of claim 1, wherein, outside the recurring time intervals:
the electric voltage at least one of applied and set by the first arrangement is at least one of smaller than within the recurring time intervals and is at least nearly zero;
the first arrangement is electrically separated from at least one of the at least one first electrode and the at least one reference electrode outside of the recurring time intervals.
9. The sensor element of claim 1, wherein the second arrangement includes at least one of:
a current measuring device for one of point-by-point recording and continuous recording of the electric current during the recurring time intervals; and
an integrator circuit for recording an integral of the electric current, the integrator circuit forming the integral during a measuring interval at least one of lying within a recurring time interval and coinciding with the recurring time interval.
10. The sensor element of claim 1, wherein one of the following is satisfied:
the at least one first electrode is at least one of supplied with a storage arrangement to store temporarily the at least one gas component to be analyzed; and
the storage arrangement to store temporarily the at least one gas component to be analyzed is provided in a vicinity of the at least one first electrode.
11. The sensor element of claim 10, wherein the storage arrangement includes one of a layer applied on the at least one first electrode, and a material integrated into the at least one first electrode, the layer and material including at least one of barium oxide, barium carbonate and cerium oxide.
12. The sensor element of claim 10, wherein the storage arrangement enriches the at least one gas component to be analyzed outside of the recurring time intervals.
13. A method for determining a concentration of at least one gas component in a gas mixture using a sensor element that includes at least one first electrode exposed to the at least one gas component to be analyzed, at least one reference electrode electrically interconnected by a first solid electrolyte to the at least one first electrode, comprising:
at least one of applying and setting an electric voltage between the at least one first electrode and the at least one reference electrode only during recurring time intervals due to a chemical reaction proceeding at the at least one first electrode; and
measuring an electric current at least one of continuously and non-continuously, between the at least one first electrode and the one of at least one reference electrode and a counterelectrode, in response to the at least one of applying and setting an electric voltage between the at least one first electrode and the at least one reference electrode due to a chemical reaction proceeding at the at least one first electrode, the concentration of the at least one gas component in the gas mixture being determinable from the electric current;
wherein the measuring of the electric current between the at least one first electrode and the counterelectrode is performed at least intermittently within the recurring time intervals.
14. The method of claim 13, wherein the recurring time intervals recur periodically and have a length of 1 ms to 100 ms, and pause intervals of 1 ms to 100 ms lie between the recurring time intervals.
15. The method of claim 13, wherein at the at least one first electrode, the at least one gas component includes nitrogen oxide to be analyzed, and reacts to form oxygen and nitrogen, the oxygen thus formed being removable through the first solid electrolyte via the electric voltage at least one of applied and set during the recurring time intervals.
16. The method of claim 13, wherein a length of the recurring time intervals is selected so that the electric current between the at least one first electrode and the at least one reference electrode, measurable during the recurring time intervals as a function of time, is greater than a limiting current measurable at a constant electric voltage at a corresponding time.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

I claim:

1. A magnetic resonance apparatus having a single-circuit cooling circulation system operated with a coolant for cooling a gradient coil system so that the temperature of at least one surface of the gradient coil system remains within a prescribable temperature range, said cooling circulation system having a dimensioning performance corresponding to an average ohmic dissipated power of the gradient system, the cooling circulation system containing a heat exchanger that can at least be switched on and off, the heat exchanger having a high-frequency onoff switching clock, the gradient coil system having a high average specific heat capacity and the coolant having an operating temperature range proceeding beyond the prescribable temperature range.
2. A magnetic resonance apparatus according to claim 1, wherein the period of the onoff switching clock is less than ten minutes.
3. A magnetic resonance apparatus according to claim 1, wherein the average specific heat capacity is greater than approximately 1 JgK.
4. A magnetic resonance apparatus according to claim 1, wherein the gradient coil system comprises a casing of casting resin.
5. A magnetic resonance apparatus according to claim 1, wherein the heat exchanger is an air-cooled heat exchanger of an interior construction.
6. A magnetic resonance apparatus according to claim 1, wherein the heat exchanger contains a ventilator that is fashioned at least to be switched on and off.
7. A magnetic resonance apparatus according to claim 1, wherein the heat exchanger contains a refrigeration system that contains a refrigeration compressor that is constructed at least to be switched on and off.
8. A magnetic resonance apparatus according to claim 1, wherein the coolant is a cooling fluid.
9. A magnetic resonance apparatus according to claim 8, wherein the cooling fluid is water.
10. A magnetic resonance apparatus according to claim 1, wherein the cooling circulation system contains a temperature sensor for determining a run-up temperature, the cooling circulation system includes a two-point regulator for regulating variables in the run-up temperature and for manipulating variables and which operates the onoff switching of the heat exchanger and the two-point regulator comprises a small switching difference for achieving a high-frequency onoff switching clock.
11. A magnetic resonance apparatus according to claim 1, wherein the magnetic resonance apparatus comprises a permanent magnet as a basic field magnet and the gradient coil system is arranged at the permanent magnet.