Author: claimsbot

1. Vector for expressing and selecting random peptide sequences of a genome of an organism, characterized in that it comprises
three restriction sites RS1, RS2 and RS3, which are unique in the vector, RS3 being located downstream relative to RS1 and upstream relative to RS2,
an insert coding for a random peptide inserted in RS3, said insert being a fragment with a length of 20 to 500 bp of said genome,
a selection marker gene located downstream of RS2 and
the random peptide sequence and the selection marker gene being expressable in frame to form a fusion protein comprised of the random peptide and the selection marker.
2. Vector according to claim 1, characterized in that it comprises a leader sequence upstream to RS1 suitable for being expressed in frame with the random peptide and the selection marker and suitable for directing the fusion protein comprised of the leader sequence, the random peptide and the selection marker to a predetermined location.
3. Vector according to claim 1 or 2, characterized in that RS1 and RS2 are 8 bp cutting restriction enzyme sites.
4. Vector according to any one of claims 1 to 3, characterized in that cutting RS3 results in blunt ends.
5. Vector according to any one of claims 1 to 4, characterized in that the random peptide sequence is derived from the genome of a pathogen.
6. Vector according to any one of claims 1 to 5, characterized in that the random peptide sequence has a length of 100 to 300 bp.
7. Vector according to any one of claims 1 to 6, characterized in that it comprises an in frame linker sequence between the random peptide sequence and the selection marker gene.
8. Vector according to any one of claims 1 to 7, characterized in that the selection marker is an antibiotic resistance.
9. Vector according to any one of claims 1 to 8, characterized in that the selection marker is -lactamase.
10. Vector according to any one of claims 1 to 9, characterized in that it contains an OmpA leader sequence.
11. Vector according to any one of claims 1 to 10, characterized in that the random peptide sequence is derived from a viral pathogen.
12. Vector according to any one of claims 1 to 11, characterized in that the random peptide sequence is a fragment of HAV, HBV, HCV, HIV-1, HIV-2, EBV, HTLV-I or HTLV-II.
13. Vector according to any one of claims 1 to 10, characterized in that the random peptide sequence is derived from a bacterial pathogen, especially from S. aureus, M. tuberculosis, C. pneumoniae, S. typhimurium, Y. pestis or S. epidermidis.
14. Vector according to any one of claims 1 to 10, characterized in that the random peptide sequence is derived from a eukaryotic pathogen, especially from T. brucei.
15. Method for selecting random peptide sequences of a genome of an organism comprising the following steps:
providing a vector comprising three restriction sites RS1, RS2 and RS3, which are unique in the vector, RS3 being located downstream relative to RS1 and upstream relative to RS2, and a selection marker gene located downstream of RS2 and optionally a leader sequence being located upstream of RS1,
inserting a library of random peptide sequences into RS3 to create a vector library, said inserts being fragments with a length of 20 to 500 bp of said genome,
introducing the vector library into a suitable host, which is capable of expressing a fusion protein comprised of random peptide and selection marker, to create a host library,
cultivating said host library on a medium selective with respect to the selection marker, thereby selecting the host individuals which express said fusion protein.
16. Method according to claim 15, characterized in that the vector of the selected host individuals is isolated and cut with RS1 and RS2 cutting restriction enzymes to obtain a fragment containing the random peptide sequence in a defined reading frame and orientation.
17. Method according to claim 16, characterized in that the fragment is inserted into another vector which has been cut with RS1 and RS2 cutting restriction sites to obtain an RS1RS2 insertion site.
18. Method according to any one of claims 15 to 17, characterized in that the cultivation medium contains an antibiotic and the selection marker is an antibiotic resistance.
19. Expressionselection vector library characterized in that in a vector according to claims 1 to 14 a library of random peptide sequences is inserted.
20. Vector system comprising a vector according to any one of claims 1 to 14 and a second vector comprising an RS1RS2 insertion site.
21. Vector system according to claim 20, characterized in that said second vector is an expression vector allowing expression of DNA fragments inserted in said RS1RS2 insertion site.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An information medium comprising:
a plurality of layers, each of the plurality of layers includes:
a first area including a disc information area having disc information stored thereon and a second area including a data area;
a first track pitch in the first area that is different from a second track pitch in the second area; and
a transition area of track pitches provided between a first track in the first area and a second track in the second area,
wherein each first area of the plurality of layers is provided at an about the same radial position,
each second area of the plurality of layers is provided at an about the same radial position,
transition of track pitches in the transition area is completed within 100 tracks, and
wherein a difference between the first track pitch and the second track pitch is less than or equal to 15%.
2. An information medium according to claim 1, wherein the information medium is a reproduction-only disc.
3. An information processing method for performing at least one of reproduction and recording of information for an information medium, the information medium including a plurality of layers, each of the plurality of layers includes: a first area including a disc information area having disc information stored thereon and a second area including a data area; a first track pitch in the first area that is different from a second track pitch in the second area; and a transition area of track pitches provided between a first track in the first area and a second track in the second area, wherein each first area of the plurality of layers is provided at an about the same radial position, each second area of the plurality of layers is provided at an about the same radial position, transition of track pitches in the transition area is completed within 100 tracks and wherein a difference between the first track pitch and the second track pitch is less than or equal to 15%,
the information processing method comprising:
reproducing the disc information; and
reproducing or recording the information for the information medium based on the reproduced disc information.
4. An information processing method according to claim 3, wherein the information medium is a reproduction-only disc.

What is claimed is:

1. A plastic container comprising:
a tube of a plastic laminate;
a bottom wall formed so as to be joined to an inner circumference of a lower end part of the tube by an insert injection molding means;
a hoop formed on an outer circumference of an upper end part of the tube by an insert injection molding means; and
a top cover provided with a spout and bonded to an upper end surface of the hoop.
2. The plastic container according to claim 1, wherein the top cover is provided with projections in a part thereof corresponding to the hoop, and the projections are crushed by an ultrasonic means to bond the top cover to the hoop.
3. The plastic container according to claim 1, wherein the plastic laminate is an opaque film including a metal foil.
4. The plastic container according to claim 1, wherein the plastic laminate is a transparent film.
5. A plastic container assembly comprising:
the plastic container of claim 1;
an outer container capable of containing the plastic container.
6. The plastic container assembly according to claim 5 wherein the tube of the plastic container is tapered from its upper end toward its lower end.
7. The plastic container assembly according to claim 5 or 6 further comprising a holding cap having a side wall provided with a screw thread on its inner circumference;
wherein the outer container has an open end part provided with a screw thread on its outer circumference, and the holding cap is screwed on the open end part of the outer container after inserting the plastic container in the outer container to fasten the plastic container to the outer container.
8. A method of manufacturing a plastic container comprising a tube of a plastic laminate, a bottom wall formed so as to be joined integrally to an inner circumference of a lower end part of the tube, a hoop formed so as to be joined integrally to an outer circumference of an upper end part of the tube, and a top cover provided with a spout and bonded to an upper end surface of the hoop, said method comprising the steps of:
forming the tube by rolling a plastic laminate in a tubular shape;
putting the tube on a mandrel;
inserting the mandrel holding the tube in a mold so that a first molding cavity connected to a runner formed in the mold, for molding the bottom wall of the plastic container is formed inside one end part of the tube and a second molding cavity connected to a runner formed in the mold, for molding the hoop is formed around the other end part of the tube;
injecting a molten resin through the runners into the first and the second cavity by an insert injection means to form the bottom wall and the hoop; and
bonding the top cover provided with the spout to an upper end surface of the hoop.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A wheel support bearing assembly, comprising:
an outer member having an inner peripheral surface formed with a plurality of outer raceways;
an inner member positioned inside the outer member with an annular working space defined between it and the outer member, said inner member having an outer peripheral surface formed with inner raceways in mating relation with the respective outer raceways in the outer member;
rows of rolling elements accommodated within the annular working space and rollingly received in part within the outer raceways in the outer member and in part within the inner raceways in the inner member; and
at least one sealing member fitted to one of the inner and outer members sealing one of opposite open ends of the annular working space delimited between the inner and outer members, said sealing member having an approximately uniform annular cross section and a plurality of elastic sealing lips which extend towards a sealing surface defined directly on the other of the inner and outer members or defined on a sealing contact member fitted to the other of the inner and outer members, one of said elastic sealing lips extending in a direction generally axially inwardly of the annular working space and defining an axially innermost sealing lip while the other elastic sealing lips being kept in sliding contact with the sealing surface;
wherein the innermost sealing lip is a non-contact sealing lip leaving a gap between a free end thereof and the sealing surface of the sealing contact member, a size of the entire gap gradually increases in a direction away from the sealing surface area and inwardly of the annular working space, said gap being of a size sufficient to
permit flow of air therethrough, and
prevent a lubricant from passing therethrough and to provide a non-contact sealing effect when a relative rotation takes place between the outer and inner members.
2. The wheel support bearing assembly as claimed in claim 1, wherein the at least one sealing member includes a core metal and an elastic member rigidly mounted on the core metal and wherein all of the elastic sealing lips are an integral part of the elastic member.
3. The wheel support bearing assembly as claimed in claim 1, wherein the gap is defined in a radial direction between a free end face of the axially innermost sealing lip and the sealing surface.
4. The wheel support bearing assembly as claimed in claim 3, wherein the free end of the axially innermost sealing lip extends inwardly of the annular working space between the inner and outer members.
5. The wheel support bearing assembly as claimed in claim 1, wherein the free end face of the axially innermost sealing lip which confronts the sealing surface has a width, as measured in a direction across a thickness of the axially innermost sealing lip, which is greater than that of any one of the remaining elastic sealing lips.
6. The wheel support bearing assembly as claimed in claim 1, wherein the sealing contact member includes a cylindrical wall and an radially upright wall protruding radially outwardly from the cylindrical wall so as to render the sealing contact member to represent a generally L-sectioned configuration, and wherein the innermost elastic sealing lip confronts a first portion of the sealing surface defined on an outer peripheral surface of the cylindrical wall and at least one of the remaining elastic sealing lips is held in sliding contact with a second portion of the sealing surface defined on the radially upright wall that confronts the sealing member.
7. The wheel support bearing assembly as claimed in claim 1, further comprising a ring-shaped multi-pole magnet secured to the sealing contact member, said multi-pole magnet magnetized to have a plurality of opposite magnetic poles alternating with each other in a direction circumferentially thereof.
8. The wheel support bearing assembly as claimed in claim 1, wherein the inner member has one end formed with a flange which extends radially outwardly beyond a level of an outer periphery of a cylindrical portion of the outer member and wherein the sealing member is used to seal one of the opposite open ends of the annular working space adjacent such flange.
9. A wheel support bearing assembly, comprising:
inner and outer members defining an annular working space therebetween, and accommodating lubricated rolling elements within the annular working space; and
a sealing member fitted to one of the inner or outer members and sealing an open end of the annular working space, the sealing member having
an approximately uniform annular cross section,
at least one elastic sealing lip maintaining sliding contact with a sealing surface defined on the remaining one of the inner or outer members, or on a sealing contact member fitted to the remaining one of the inner or outer members, and
an axially innermost elastic sealing lip extending axially inwardly of the annular working space and defining a gap between an end thereof and the sealing surface, a size of the entire gap gradually increasing in a direction away from the sealing surface and inwardly of the annular working space, the gap being sized to permit flow of air therethrough and provide a non-contact sealing effect to prevent a lubricant from exiting therethrough during relative rotation between the inner and outer members.