1. A method, comprising:
obtaining dielectric dispersion measurements on core samples having matrix material from a formation of interest;
determining a water content of the core samples by analyzing at least a portion of the core samples;
determining dielectric petrophysical properties of the core samples using the determined water content and the dielectric dispersion measurements;
estimating an effective matrix permittivity of the core sample matrix material using the determined dielectric petrophysical properties;
determining a mineralogy composition of the core sample by analyzing at least a portion of the core samples;
employing a volumetric mixing law using the determined effective matrix permittivity and the determined mineralogy composition; and
determining a dry permittivity for each mineral identified by the volumetric mixing law.
2. The method of claim 1, wherein the dielectric dispersion measurements are made using a network analyzer.
3. The method of claim 1, wherein the determining a water content comprises using a retort technique or a thermo-gravimetric technique.
4. The method of claim 1, wherein the estimating an effective matrix permittivity comprises inverting measured permittivity and conductivity dispersions.
5. The method of claim 1, wherein the determining a mineralogy composition comprises using a Fourier transform infrared technique or an X-ray diffraction technique.
6. The method of claim 1, wherein the employing a volumetric mixing law comprises using a complex refractive index technique.
7. A method, comprising:
determining a mineralogy composition of a formation of interest using measurements on one or more core samples or one or more downhole logging tool logs;
determining a dry permittivity for each identified mineral;
employing a volumetric mixing law using the determined mineralogy composition and the determined dry permitivities;
determining an effective matrix permittivity using results from the volumetric mixing law;
acquiring dielectric dispersion measurements of the subject formation using the one or more core samples or the one or more downhole logging tool logs;
producing a dielectric petrophysical model using the dielectric dispersion measurements and the effective matrix permittivity;
estimating a water saturation based on the dielectric petrophysical model;
acquiring nuclear magnetic resonance (NMR) T2 measurements having short echo spacings;
generating a NMR petrophysical model based on the NMR T2 measurements;
determining a total porosity based on the generated NMR petrophysical model; and
making a total gas-in-place estimate using the determined total porosity and the estimated water saturation.
8. The method of claim 7, wherein the determining a mineralogy composition comprises using a Fourier transform infrared technique, an X-ray diffraction technique, or downhole measurements from a spectroscopic tool.
9. The method of claim 7, wherein the employing a volumetric mixing law comprises using a complex refractive index technique.
10. The method of claim 7, wherein the estimating an effective matrix permittivity comprises using the determined dry permitivities for each identified mineral.
11. The method of claim 7, wherein acquiring dielectric dispersion measurements comprises using a spectrometer on the one or more core samples or a multifrequency dielectric tool downhole.
12. The method of claim 7, wherein the estimating a water saturation comprises using inverted dielectric dispersion measurements and the effective matrix permittivity.
13. The method of claim 7, wherein the estimated water saturation includes free clay water, capillary clay water, bound clay water, and structural water.
14. The method of claim 7, wherein the short echo spacings are approximately 0.13 milliseconds or less.
15. The method of claim 7, wherein the total porosity based on the generated NMR petrophysical model provides an estimate of the total volume of the fluid present in the sampled region.
16. The method of claim 7, wherein the total volume of the fluid in the sampled region includes one or more of the capillary bound water, the free water, the clay bound water, the free gas, and the adsorbed gas present in the sampled region.
17. The method of claim 7, wherein making a total gas-in-place estimate comprises subtracting a water volume estimated from the dielectric measurements from the total fluid volume determined using the generated NMR petrophysical model.
18. An system, comprising:
a multifrequency dielectric dispersion measurement tool;
a nuclear magnetic resonance (NMR) measurement tool; and
a processor capable of performing the following operations:
determining a mineralogy composition of a formation of interest using measurements on core samples or a downhole tool response;
determining a dry permittivity for each identified mineral;
employing a volumetric mixing law using the determined mineralogy composition and the determined dry permitivities;
determining an effective matrix permittivity using results from the volumetric mixing law;
acquiring dielectric dispersion measurements of the subject formation using the measurements on the core samples or the downhole tool response;
producing a dielectric petrophysical model using the dielectric dispersion measurements and the effective matrix permittivity;
estimating a water saturation based on the dielectric petrophysical model;
acquiring nuclear magnetic resonance (NMR) T2 measurements having short echo spacings on the core samples or the formation of interest;
generating a NMR petrophysical model based on the NMR T2 measurements;
determining a total porosity based on the generated NMR petrophysical model; and
making a total gas-in-place estimate using the determined total porosity and the estimated water saturation.
19. The system of claim 18, wherein the dielectric dispersion measurement tool and the NMR measurement tool are both capable of operating in a laboratory or downhole in a well.
20. The system of claim 18, wherein the NMR measurement tool is capable of making measurements having short echo spacings.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. An isolated nucleic acid molecule comprising a polynucleotide having a sequence at least 95% identical to a sequence selected from the group consisting of:
(a) a nucleotide sequence encoding a human sel-10 polypeptide having the complete amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7, or as encoded by the cDNA clone contained in ATCC Deposit No.98978;
(b) a nucleotide sequence encoding a human sel-10 polypeptide having the complete amino acid sequence selected from the group consisting of SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or as encoded by the cDNA clone contained in ATCC Deposit No. 98979; and
(c) a nucleotide sequence complementary to the nucleotide sequence of (a) or (b).
2. An isolated nucleic acid molecule comprising polynucleotide which hybridizes under stringent conditions to a polynucleotide having the nucleotide sequence in (a), (b), or (c) of claim 1.
3. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:3.
4. The nucleic acid molecule of claim 3, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 45-1928 of SEQ ID NO:1.
5. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10polypeptide having the complete amino acid sequence of SEQ ID NO:4.
6. The nucleic acid molecule of claim 5, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 150-1928 of SEQ ID NO:1.
7. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:5.
8. The nucleic acid molecule of claim 7, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 267-1928 of SEQ ID NO:1.
9. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:6.
10. The nucleic acid molecule of claim 9, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 291-1928 of SEQ ID NO:1.
11. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(a) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:7.
12. The nucleic acid molecule of claim 11, wherein said polynucleotide molecule of 1(a) comprises the nucleotide sequence of residues 306-1928 of SEQ ID NO:1.
13. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(b) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:8.
14. The nucleic acid molecule of claim 13 wherein said polynucleotide molecule of 1(b) comprises the nucleotide sequence of residues 180-1949 of SEQ ID NO:2.
15. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(b) encodes a human sel-10polypeptide having the complete amino acid sequence of SEQ ID NO:9.
16. The nucleic acid molecule of claim 15, wherein said polynucleotide molecule of 1(b) comprises the nucleotide sequence of residues 270-1949 of SEQ ID NO:2.
17. The nucleic acid molecule of claim 1, wherein said polynucleotide of 1(b) encodes a human sel-10 polypeptide having the complete amino acid sequence of SEQ ID NO:10.
18. The nucleic acid molecule of claim 17, wherein said polynucleotide molecule of 1(b) comprises the nucleotide sequence of residues 327-1949 of SEQ ID NO:2.
19. A vector comprising the nucleic acid molecule of claim 1.
20. The vector of claim 19, wherein said nucleic acid molecule of claim 1 is operably linked to a promoter for the expression of a sel-10 polypeptide.
21. A host cell comprising the vector of claim 19.
22. The host cell of claim 21, wherein said host is a eukaryotic host.
23. A method of obtaining a sel-10 polypeptide comprising culturing the host cell of claim 22 and isolating said sel-10 polypeptide.
24. An isolated sel-10 polypeptide comprising
(a) an amino acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7, or as encoded by the cDNA clone contained in ATCC Deposit No. 98978;
(b) an amino acid sequence selected from the group consisting of SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or as encoded by the cDNA clone contained in ATCC Deposit No. 98979.
25. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:3.
26. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:4.
27. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:5.
28. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:6.
29. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:7.
30. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:8.
31. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:9.
32. The isolated sel-10 polypeptide of claim 24, wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:10.
33. An isolated antibody that binds specifically to the sel-10 polypeptide of claim 24.
34. A cell line having altered A processing that expresses any of the sel-10 isolated nucleic acid molecules of claim 1.
35. The cell line of claim 34, wherein said A processing is increased.
36. The cell line of claim 34, wherein said A processing is decreased.
37. The cell line of claim 34, wherein said cell line is 6myc-N-sel102.
38. The cell line of claim 34, wherein said cell line is 6myc-N-sel106.
39. A method for the identification of an agent capable of altering the ratio of A1-40A1-40A1-42 produced in any of the cell lines of claims 34, 37, and 38, comprising the steps of:
(a) obtaining a test culture and a control culture of said cell line;
(b) contacting said test culture with a test agent;
(c) measuring the levels of A1-40 and A1-42 produced by said test culture of step (b) and said control culture;
(d) calculating the ratio of A1-40A1-40A1-42 for said test culture and said control culture from the levels of A1-40 and A1-42 measured in step (c); and
(e) comparing the ratio of A1-40A1-40A1-42 measured for said test culture and said control culture in step (d);
whereby a determination that the ratio of A1-40A1-40A1-42 for said test culture is higher or lower than ratio of A1-40A1-40A1-42 for said control culture indicates that said test agent has altered the ratio of A1-40A1-40A1-42.
40. The method of claim 39, wherein said ratio of A1-40A1-40A1-42 is increased by said test agent.
41. The method of claim 39, wherein said ratio of A1-40A1-40A1-42 is decreased by said test agent.