1. A method for increasing a RNA interference (RNAi) effect of a molecule or composition that reduces expression of a selected gene or genes by RNA interference in cell(s) or an organism comprising
contacting the cell(s) or organism with (1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition and (2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference.
2. The method of claim 1, wherein the cell(s) or organism is contacted with the first active component
(i) before or after contacting the cell(s) or organism with the second active component,
(ii) simultaneously with contacting the cell(s) or organism with the second active component, or
(iii) before, simultaneously with andor after contacting the cell(s) or organism with the second active component.
3. The method of claim 1, wherein the contacting comprises applying or spraying the second active component and the effective amount of the first active component onto the target cell(s) or target organism or on food for the target organism.
4. A method for protecting plants or foodstuffs or substances from pest infestation or pathogen infection comprising
contacting the plant or the soil on which the plant is rooted, or foodstuff or substance with (1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition and (2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference.
5. The method of claim 4, wherein the plant or the soil on which the plant is rooted, or foodstuff or substance is contacted with the first active component
(i) before or after contacting the plant or the soil on which the plant is rooted, or foodstuff or substance with the second active component,
(ii) simultaneously with contacting the plant or the soil on which the plant is rooted, or foodstuff or substance with the second active component, or
(iii) before, simultaneously with andor after contacting the plant or the soil on which the plant is rooted, or foodstuff or substance with the second active component.
6. The method of claim 4, wherein the contacting comprises applying or spraying the second active component and the effective amount of the first active component onto the plant or foodstuff or substance.
7. A method for treating a plant infection by a pathogen or plant infestation by a pest comprising
contacting the plant or the soil in which the plant is rooted with (1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition and (2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference.
8. A method for increasing crop yield or reducing a decline in crop yield that results from pest infestation andor pathogen infection comprising
contacting the plant or the soil wherein the plant is rooted with (1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition and (2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference.
9. The method of claim 7, wherein the plant or the soil on which the plant is rooted is contacted with the first active component
(i) before or after contacting the plant or the soil on which the plant is rooted with the second active component,
(ii) simultaneously with contacting the plant or the soil on which the plant is rooted with the second active component, or
(iii) before, simultaneously with andor after contacting the plant or the soil on which the plant is rooted with the second active component.
10. The method of claim 7, wherein the contacting comprises applying or spraying the second active component and the effective amount of the first active component onto the plant or onto the soil wherein the plant is rooted.
11. The method of claim 1, wherein the first active component is as recited in claim 20 and the second active component comprises at least one RNA molecule of which at least a portion is a double-stranded (dsRNA) molecule.
12. The method of claim 1, wherein the first active component is as recited in claim 20 and the second active component is as recited in claim 20.
13. The method of claim 1, wherein the first active component and the second active component are combined in a composition as recited in claim 20.
14. A method for protecting a human or an animal from pest infestation or pathogen infection comprising
administering to the human or animal (1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition and (2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference.
15. A method for treating a pest infestation or pathogen infection of a human or an animal, comprising administering to the human or animal an effective amount of
(1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition, and
(2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference.
16. The method of claim 14, wherein the human or animal is administered the first active component
(i) before or after administering the second active component to the human or animal,
(ii) simultaneously with administering the second active component to the human or animal, or
(iii) before, simultaneously with andor after administering the second active component to the human or animal.
17. The method of claim 14, wherein the administering comprises topical, parenteral, enteral, transdermal, cutaneous, subcutaneous, intravenous, intraperitoneal, intramuscular or oral administration.
18. The method of claim 14, wherein the first active component is as recited in claim 20 and the second active component is as recited in claim 20.
19. The method of claim 14, wherein the first active component and the second active component are combined in a composition as recited in claim 20.
20. A composition comprising
a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans, wherein the amount of one or more sulfated polysaccharides andor glycosaminoglycans is effective to increase the RNAi effect of a molecule(s) or a composition on a selected gene or genes and
a second active component comprising one or more prokaryotic cells or eukaryotic cells or host organisms that express double-stranded RNA molecule(s) that reduce expression of a selected gene or genes by RNA interference (RNAi) in a target cell or target organism.
21. The composition of claim 20, wherein the first active component is a composition of one or more sulfated polysaccharides andor glycosaminoglycans.
22. The composition of claim 20, wherein the first active component is dextran sulfate.
23. The composition of claim 20, wherein the first active component is fucoidan.
24. The composition of claim 20, wherein the first active component is heparin.
25. The composition of claim 22, wherein the dextran sulfate has an average molecular weight of between 8 and 40 kilodaltons.
26. The composition of claim 20, wherein the one or more prokaryotic cells or eukaryotic cells or host organisms express at least one RNA molecule of which at least a portion is a double-stranded (dsRNA) molecule.
27. The composition of claim 20, wherein the one or more prokaryotic cells or eukaryotic cells or host organisms comprise at least one expression vector that expresses at least one RNA molecule of which at least a portion is a double-stranded RNA (dsRNA) molecule.
28. The composition of claim 26, wherein the dsRNA molecule is a dsRNA molecule consisting of at least two separate strands, an siRNA molecule or a hairpin molecule.
29. The composition of claim 27, wherein the expression vector comprises at least one regulatory sequence operably linked to the nucleotide sequence encoding the at least one RNA molecule.
30. The composition of claim 20, wherein the prokaryotic cell is a bacterial cell chosen from the group comprising Gram positive and Gram negative cells comprising Escherichia spp. (e.g. E. coli), Bacillus spp. (e.g. B. thuringiensis), Rhizobium spp., Lactobacilllus spp., and Lactococcus spp.
31. The composition of claim 30, wherein the bacterial cell is inactivated by heat or by chemical treatment.
32. The composition of claim 20, wherein the eukaryotic cell is chosen from the group comprising mammalian cells and non-human mammalian cells.
33. The composition of claim 20, wherein the eukaryotic cell is a yeast cell.
34. The composition of claim 20, wherein at least one strand of the dsRNA comprises a nucleotide sequence that is complementary to a portion of the nucleotide sequence of a selected gene or genes from the target cell or target organism.
35. The composition of claim 20, wherein said gene or genes is essential for the viability, growth, development or reproduction of the target cell or target organism.
36. The composition of claim 20, wherein said gene or genes in the second active component comprises (a) the sequence of any of SEQ ID NOs 1 to 6, (b) the RNA equivalent thereof, (3) the complement of (a) or (b), or a fragment of any of (a) to (c) consisting of at least 17 contiguous nucleotides of any of (a) to (c).
37. The composition of claim 20, wherein the target organism is a pest species, a pathogen species, or a cell thereof.
38. The composition of claim 37, wherein said pest species or pathogen species is a fungus, an insect or a nematode.
39. A composition comprising
a first active component comprising an amount of dextrane sulfate having a molecular weight between 5 and 2000 kDA, preferably between 8 and 40 kDa, wherein the amount of dextrane sulfate is effective to increase the RNAi effect of a molecule(s) or a composition on a selected gene or genes and
a second active component comprising at least one RNA molecule of which at least a portion is a double-stranded RNA (dsRNA) molecule.
40. The composition of claim 39 wherein the at least one RNA molecule in the second active compound is as recited in claim 28.
41. The composition of claim 20, wherein the first active component is present in an amount of 0.000001% to 99% by weight of the composition (WW), preferably 0.00001% to 99% by weight (WW), more preferably, 0.0001% to 99% by weight (WW), still more preferably 0.0002% to 99% by weight (WW).
42. The composition of claim 20, wherein the second active component is present in an amount of 0.0000000001% to 99% by weight (WW) of the composition, preferably 0.000000001% to 99% by weight (WW), more preferably 0.00000001% to 99% by weight (WW).
43. A kit comprising
a first container containing an amount of a first active component comprising one or more sulfated polysaccharides andor glycosaminoglycans as recited in claim 20, wherein the amount of one or more sulfated polysaccharides andor glycosaminoglycans is effective to increase the RNAi effect of a molecule(s) or a composition, and
a second container containing a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference (RNAi) in a target cell or target organism as recited in claim 20.
42. A method for making a RNAi composition comprising combining
a first active component comprising an amount of the one or more sulfated polysaccharides andor glycosaminoglycans as recited in claim 20, wherein the amount of one or more sulfated polysaccharides andor glycosaminoglycans is effective to increase the RNAi effect of a molecule(s) or a composition on a selected gene or genes, and
a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference (RNAi) in a cell or organism as recited in claim 20, to make a RNAi composition.
43. A medicament comprising an effective amount of:
(1) a first active component comprising an amount of one or more sulfated polysaccharides andor glycosaminoglycans that is effective to enhance the RNAi effect of a molecule(s) or a composition, wherein said first active compound is recited claim 20, and
(2) a second active component comprising a molecule(s) or a composition that reduces expression of a selected gene or genes by RNA interference, wherein said second active compound is recited in claim 20.
44. The medicament of claim 43 which is formulated for topical, parenteral, enteral, transdermal, cutaneous, subcutaneous, intravenous, intraperitoneal, intramuscular or oral administration.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A method of employing a Rake receiver in a wireless radio access system to a radio uplink link which results in a significant increase in data transference in a spread modulation signal, the method comprising:
(a) receiving a spread modulation signal and presenting it to a plurality of Rake fingers, wherein each Rake finger processes the spread modulation signal, each Rake finger delaying the spread modulation signal by a differing amount of time;
(b) multiplying the delayed spread modulation signal with a first reference signal producing a control bit signal;
(c) multiplying the delayed spread modulation signal with a second reference signal producing a data signal;
(d) delaying said data signal producing a delayed data signal;
(e) multiplying said delayed data signal with weights produced by a frequency offset estimator and a complex weight gain generator to produce a first set of data; and
(f) summing the outputs of all of the Rake fingers, producing data symbols and control bit symbols.
2. The method of claim 1 wherein the frequency offset estimator performs a frequency offset estimation determined by averaging blocks of pilot signals.
3. The method of claim 2 wherein said frequency offset estimation is determined using a sliding window averaging algorithm.
4. The method of claim 2 wherein said frequency offset estimation is determined using a recursive filter.
5. A Rake receiver used in a wireless communications system, the Rake receiver comprising:
(a) means for receiving a spread modulation signal and presenting it to a plurality of Rake fingers, wherein each Rake finger processes the spread modulation signal, each Rake finger delaying the spread modulation signal by a differing amount of time;
(b) means for multiplying the delayed spread modulation signal with a first reference signal producing a control bit signal;
(c) means for multiplying the delayed spread modulation signal with a second reference signal producing a data signal;
(d) means for delaying said data signal producing a delayed data signal;
(e) means for multiplying said delayed data signal with weights produced by a frequency offset estimator and a complex weight gain generator to produce a first set of data; and
(f) means for summing the outputs of all of the Rake fingers, producing data symbols and control bit symbols.
6. The receiver of claim 5 wherein the frequency offset estimator performs a frequency offset estimation determined by averaging blocks of pilot signals.
7. The receiver of claim 5 wherein said frequency offset estimation is determined using a sliding window averaging algorithm.
8. The receiver of claim 5 wherein said frequency offset estimation is determined using a recursive filter.
9. A wireless receivertransmitter unit (WTRU) used in a wireless communications system, comprising:
(a) means for receiving a spread modulation signal and presenting it to a plurality of Rake fingers, wherein each Rake finger processes the spread modulation signal, each Rake finger delaying the spread modulation signal by a differing amount of time;
(b) means for multiplying the delayed spread modulation signal with a first reference signal producing a control bit signal;
(c) means for multiplying the delayed spread modulation signal with a second reference signal producing a data signal;
(d) means for delaying said data signal producing a delayed data signal;
(e) means for multiplying said delayed data signal with weights produced by a frequency offset estimator and a complex weight gain (CWG) generator to produce a first set of data; and
(f) means for summing the outputs of all of the Rake fingers, producing data symbols and control bit symbols.
10. The WTRU of claim 9 wherein the frequency offset estimator performs a frequency offset estimation determined by averaging blocks of pilot signals.
11. The WTRU of claim 10 wherein said frequency offset estimation is determined using a sliding window averaging algorithm.
12. The WTRU of claim 10 wherein said frequency offset estimation is determined using a recursive filter.
13. A Rake receiver used in a wireless communications system, the Rake receiver comprising:
(a) a first combiner;
(b) a second combiner; and
(c) a plurality of Rake fingers for receiving and processing a spread modulation signal, each Rake finger comprising:
(i) a first despreader having a first input for receiving the spread modulation signal, a second input for receiving a pilot signal and an output for outputting despread pilot symbols;
(ii) a second despreader having a first input for receiving the spread modulation signal, a second input for receiving a data signal and an output for outputting despread data symbols;
(iii) a data bit processor having an input for receiving the despread pilot symbols and an output for outputting processed pilot symbols to be combined in the first combiner with other similarly processed pilot symbols outputted by other ones of the Rake fingers;
(iv) a frequency offset estimator having an input for receiving the despread pilot symbols and an output for outputting a frequency offset estimation signal;
(v) a complex weight gain generator in communication with the output of the first despreader and the output of the frequency offset estimator, the complex weight gain generator having a first input for receiving the despread pilot symbols, a second input for receiving the frequency offset estimation signal, and an output for outputting weighted symbols;
(vi) a delay element in communication with the output of the second despreader for delaying the despread data symbols, the delay element having an output for outputting the delayed despread data symbols; and
(vii) a multiplier in communication with the output of the delay element and the output of the complex weight gain generator, the multiplier having a first input for receiving the delayed despread data symbols and a second input for receiving the weighted symbols and outputting processed data symbols to be combined in the second combiner with other similarly processed data symbols outputted by other ones of the Rake fingers.
14. The Rake receiver of claim 13 wherein the pilot signal comprises a dedicated physical common channel (DPCCH).
15. The Rake receiver of claim 14 wherein the first despreader outputs despread DPCCH symbols.
16. The Rake receiver of claim 13 wherein the data signal comprises a dedicated physical data channel (DPDCH).
17. The Rake receiver of claim 16 wherein the second despreader outputs despread DPDCH symbols.
18. The Rake receiver of claim 14 wherein the data bit processor outputs transmit power control (TPC) and feedback information (FBI) used for closed loop transmission diversity.
19. An integrated circuit (IC) which processes spread modulation signals, the IC comprising:
(a) an input for receiving a spread modulation signal;
(b) a first combiner;
(c) a second combiner; and
(d) a plurality of Rake fingers for receiving and processing a spread modulation signal, each Rake finger comprising:
(i) a first despreader having a first input for receiving the spread modulation signal, a second input for receiving a pilot signal and an output for outputting despread pilot symbols;
(ii) a second despreader having a first input for receiving the spread modulation signal, a second input for receiving a data signal and an output for outputting despread data symbols;
(iii) a data bit processor having an input for receiving the despread pilot symbols and an output for outputting processed pilot symbols to be combined in the first combiner with other similarly processed pilot symbols outputted by other ones of the Rake fingers;
(iv) a frequency offset estimator having an input for receiving the despread pilot symbols and an output for outputting a frequency offset estimation signal;
(v) a complex weight gain generator in communication with the output of the first despreader and the output of the frequency offset estimator, the complex weight gain generator having a first input for receiving the despread pilot symbols, a second input for receiving the frequency offset estimation signal, and an output for outputting weighted symbols;
(vi) a delay element in communication with the output of the second despreader for delaying the despread data symbols, the delay element having an output for outputting the delayed despread data symbols; and
(vii) a multiplier in communication with the output of the delay element and the output of the complex weight gain generator, the multiplier having a first input for receiving the delayed despread data symbols and a second input for receiving the weighted symbols and outputting processed data symbols to be combined in the second combiner with other similarly processed data symbols outputted by other ones of the Rake fingers.
20. The IC of claim 19 wherein the pilot signal comprises a dedicated physical common channel (DPCCH).
21. The IC of claim 20 wherein the first despreader outputs despread DPCCH symbols.
22. The IC of claim 19 wherein the data signal comprises a dedicated physical data channel (DPDCH).
23. The IC of claim 22 wherein the second despreader outputs despread DPDCH symbols.
24. The IC of claim 19 wherein the data bit processor outputs transmit power control (TPC) and feedback information (FBI) used for closed loop transmission diversity.