Author: claimsbot

1. A system for collecting, re-pressurizing, purifying and re-using helium gas used as a carrier gas for a gas chromatograph, the system comprising:
(i) a flexible bladder comprising a bladder interior, the bladder interior fluidically coupled to at least one of a split vent and a septum purge vent of the gas chromatograph so as to receive helium-bearing gas output from the at least one of said vents;
(ii) a compartment comprising a compartment interior within which the bladder is contained;
(iii) a source of pressurized air or gas fluidically coupled to the compartment interior and operable so as to supply said pressurized air or gas into the compartment interior so as to compress the flexible bladder containing the helium-bearing gas;
(iv) a gas reservoir fluidically coupled to the bladder interior so as to receive the helium bearing gas from the interior of the compressed bladder; and
(v) at least one gas purification module comprising a pyrolysis furnace that is fluidically coupled to the gas reservoir so as to receive the helium-bearing gas from the gas reservoir and operable to remove gas components other than helium from the helium-bearing gas,
wherein an output of the at least one gas purification module is fluidically coupled to a carrier gas inlet of the gas chromatograph.
2. A system as recited in claim 1, wherein the at least one gas purification module further comprises a molecular sieve module.
3. A system for collecting, re-pressurizing, purifying and re-using helium gas used as a carrier gas for a gas chromatograph, the system comprising:
(i) a flexible bladder comprising a bladder interior, the bladder interior fluidically coupled to at least one of a split vent and a septum purge vent of the gas chromatograph so as to receive helium-bearing gas output from the at least one of said vents;
(ii) a compartment comprising a compartment interior within which the bladder is contained;
(iii) a source of pressurized air or gas fluidically coupled to the compartment interior and operable so as to supply said pressurized air or gas into the compartment interior so as to compress the flexible bladder containing the helium-bearing gas;
(iv) a gas reservoir fluidically coupled to the bladder interior so as to receive the helium bearing gas from the interior of the compressed bladder;
(v) at least one gas purification module fluidically coupled to the gas reservoir so as to receive the helium-bearing gas from the gas reservoir and having an output that is fluidically coupled to a carrier gas inlet of the gas chromatograph, the at least one gas purification module operable to remove gas components other than helium from the helium-bearing gas and comprising a molecular sieve module having an input end configured so as to receive the helium-bearing gas from the gas reservoir and an output end;
(vi) a source of purified helium gas fluidically coupled to the output end of the molecular sieve module for providing a flow of the purified helium gas into the molecular sieve module through the output end; and
(vii) a second valve fluidically coupled between the reservoir and the input end of the molecular sieve module and configurable so as to receive the flow of the purified helium gas from the input end of the molecular sieve module and to direct said flow of the purified helium gas to an exhaust vent.
4. A system as recited in claim 3, further comprising:
(viii) a heater coupled to the molecular sieve module.
5. A system as recited in claim 3, further comprising:
(viii) a vacuum system fluidically coupled to the exhaust vent.
6. A system as recited in claim 5 wherein the vacuum system is a mass spectrometer vacuum system.
7. A system as recited in claim 3, wherein the exhaust vent is fluidically coupled to an ion source of a mass spectrometer.
8. A system as recited in claim 3, wherein the source of purified helium gas is configured so as to supply a second flow of the purified helium gas to a carrier gas inlet of the gas chromatograph.
9. A system for collecting, re-pressurizing, purifying and re-using helium as used as a carrier gas for a gas chromatograph, the system comprising:
(i) a flexible bladder comprising a low permeability metal foil-polymer laminate film and comprising a bladder interior, the bladder interior fluidically coupled to at least one of a split vent and a septum purge vent of the gas chromatograph so as to receive helium-bearing gas output from the at least one of said vents;
(ii) a compartment comprising a compartment interior within which the bladder is contained;
(iii) a source of pressurized air or gas fluidically coupled to the compartment interior and operable so as to supply said pressurized air or as into the compartment interior so as to compress the flexible bladder containing the helium-bearing gas;
(iv) a gas reservoir fluidically coupled to the bladder interior so as to receive the helium bearing gas from the interior of the compressed bladder; and
(v) at least one gas purification module fluidically coupled to the gas reservoir so as to receive the helium-bearing gas from the gas reservoir and operable to remove gas components other than helium from the helium-bearing gas,
wherein an output of the at least one gas purification module is fluidically coupled to a carrier gas inlet of the gas chromatograph.
10. A system for collecting, re-pressurizing, purifying and re-using helium gas used as a carrier gas for a gas chromatograph, the system comprising:
(i) a flexible bladder comprising a bladder interior, the bladder interior fluidically coupled to at least one of a split vent and a septum purge vent of the gas chromatograph so as to receive helium-bearing gas output from the at least one of said vents;
(ii) a compartment comprising a compartment interior within which the bladder is contained;
(iii) a source of pressurized air or gas fluidically coupled to the compartment interior and operable so as to supply said pressurized air or gas into the compartment interior so as to compress the flexible bladder containing the helium-bearing gas;
(iv) a gas reservoir fluidically coupled to the bladder interior so as to receive the helium bearing gas from the interior of the compressed bladder;
(v) at least one gas purification module fluidically coupled to the gas reservoir so as to receive the helium-bearing gas from the gas reservoir and having an output that is fluidically coupled to a carrier gas inlet of the gas chromatograph, the at least one gas purification module operable to remove gas components other than helium from the helium-bearing gas and comprising a first molecular sieve module comprising a first input end and a first output end;
(vi) a first valve fluidically coupled between the gas reservoir and the at least one purification module;
(vii) at least one additional gas purification module fluidically coupled to the first valve and operable to remove gas components other than helium from the helium-bearing gas and comprising a second molecular sieve module comprising a second input end and a second output end;
(viii) a source of purified helium gas fluidically coupled to the first output end and the second output end for providing a flow of the purified helium gas into either the first molecular sieve module through the first output end or the second molecular sieve module through the second output end;
(ix) a second valve fluidically coupled between the first valve and the first input end of the first molecular sieve module; and
(x) a third valve fluidically coupled between the first valve and the second input end of the second molecular sieve module,
wherein the first valve is configurable to direct the helium-bearing gas either to the at least one purification module or to the at least one additional gas purification module and wherein an output of the at least one additional gas purification module is fluidically coupled to a carrier gas inlet of the gas chromatograph.
11. A system for collecting, re-pressurizing, purifying and re-using helium gas used as a carrier gas for a gas chromatograph, the system comprising:
(i) a flexible bladder comprising a bladder interior, the bladder interior fluidically coupled to at least one of a split vent and a septum purge vent of the as chromatograph so as to receive helium-bearing gas output from the at least one of said vents;
(ii) a compartment comprising a compartment interior within which the bladder is contained;
(iii) a source of pressurized air or gas fluidically coupled to the compartment interior and operable so as to supply said pressurized air or gas into the compartment interior so as to compress the flexible bladder containing the helium-bearing gas;
(iv) a gas reservoir fluidically coupled to the bladder interior so as to receive the helium bearing gas from the interior of the compressed bladder;
(v) at least one gas purification module fluidically coupled to the gas reservoir so as to receive the helium-bearing gas from the gas reservoir and operable to remove gas components other than helium from the helium-bearing gas;
(vi) a first actuator operable so as to adjust the fluidic coupling between the bladder interior and the at least one of the split vent and the septum purge vent;
(vii) a second actuator operable so as to adjust the fluidic coupling between the compartment interior and the source of pressurized air or gas;
(viii) a sensor coupled to the flexible bladder for sensing a degree of inflation of the flexible bladder; and
(ix) an electronic controller electrically coupled to the first and second actuators and to the sensor, wherein the electronic controller is operable so as to send control signals to the first and second actuators in response to a signal received from the sensor,
wherein an output of the at least one gas purification module is fluidically coupled to a carrier gas inlet of the gas chromatograph.
12. A method for re-claiming helium gas output from a split vent or a septum purge vent of a gas chromatograph, comprising:
(a) receiving a flow of a gas mixture including the helium gas into the interior of a flexible bladder from at least one of the split vent and the septum purge vent;
(b) compressing the flexible bladder so as to expel the gas mixture from the bladder interior into a reservoir; and
(c) causing the gas mixture to flow from the reservoir through a pyrolysis furnace of a gas purification module, the gas purification module removing gas components other than helium from the gas mixture flowing therethrough so as to recover purified helium.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method, comprising:
providing a notification to an intended recipient of a message, the notification including a designated link operable to facilitate access to the message, the designated link including a unique identifier associated with the message;
receiving a request to access the message;
authenticating the request according to an authentication procedure, the authentication procedure including verifying whether the request corresponds to the designated link provided to the intended recipient; and
communicating the message if the request passes authentication.
2. The method of claim 1, further comprising:
sending a request for login credentials associated with a user, the request for login credentials sent in response to the request to access the message;
receiving the login credentials; and
determining that the request passes authentication if the login credentials are valid and the request corresponds to the designated link.
3. The method of claim 1, wherein verifying whether the request corresponds to the designated link comprises verifying that the request includes the unique identifier associated with the message.
4. The method of claim 1, wherein the recipient corresponds to an email account.
5. The method of claim 1, wherein:
the designated link comprises a user-readable portion and a machine-readable portion, the machine-readable portion including the unique identifier; and
the notification configured to display the user-readable portion to a user and to hide the machine-readable portion from the user.
6. The method of claim 1, wherein:
the message comprises an encrypted message; and
communicating the message comprises:
decrypting the message; and
communicating the message over a secure connection.
7. The method of claim 1, further comprising:
determining that the request fails to correspond to the designated link provided to the intended recipient; and
initiating a fallback authentication procedure.
8. One or more non-transitory computer-readable media comprising logic, the logic when executed by one or more processing units operable to perform operations comprising:
providing a notification to an intended recipient of a message, the notification including a designated link operable to facilitate access to the message, the designated link including a unique identifier associated with the message;
receiving a request to access the message;
authenticating the request according to an authentication procedure, the authentication procedure including verifying whether the request corresponds to the designated link provided to the intended recipient; and
communicating the message if the request passes authentication.
9. The media of claim 8, the logic further operable to perform the operations comprising:
sending a request for login credentials associated with a user, the request for login credentials sent in response to the request to access the message;
receiving the login credentials; and
determining that the request passes authentication if the login credentials are valid and the request corresponds to the designated link.
10. The media of claim 8, wherein verifying whether the request corresponds to the designated link comprises verifying that the request includes the unique identifier associated with the message.
11. The media of claim 8, wherein the intended recipient corresponds to an email account.
12. The media of claim 8, wherein:
the designated link comprises a user-readable portion and a machine-readable portion, the machine-readable portion including the unique identifier; and
the notification configured to display the user-readable portion to a user and to hide the machine-readable portion from the user.
13. The media of claim 8, wherein:
the message comprises an encrypted message; and
communicating the message comprises:
decrypting the message; and
communicating the message over a secure connection.
14. The media of claim 8, the logic further operable to perform the operations comprising:
determining that the request fails to correspond to the designated link provided to the intended recipient; and
initiating a fallback authentication procedure.
15. A system, comprising:
one or more processors operable to:
provide a notification to an intended recipient of a message, the notification including a designated link operable to facilitate access to the message, the designated link including a unique identifier associated with the message;
receive a request to access the message;
authenticate the request according to an authentication procedure, the authentication procedure including verifying whether the request corresponds to the designated link provided to the intended recipient; and
communicate the message if the request passes authentication.
16. The system of claim 15, the one or more processors further operable to:
send a request for login credentials associated with a user, the request for login credentials sent in response to the request to access the message;
receive the login credentials from the user; and
determine that the request passes authentication if the login credentials are valid and the request corresponds to the designated link.
17. The system of claim 15, wherein verifying whether the request corresponds to the designated link comprises verifying that the request includes the unique identifier associated with the message.
18. The system of claim 15, wherein:
the designated link comprises a user-readable portion and a machine-readable portion, the machine-readable portion including the unique identifier; and
the notification configured to display the user-readable portion to a user and to hide the machine-readable portion from the user.
19. The system of claim 15, the authentication procedure further including verifying authentication information corresponding to at least one of a username, a passcode, personal information, device information, or biometric data.
20. The system of claim 15, wherein:
the message comprises an encrypted message; and
the one or more processors further operable to:
decrypt the message; and
establish a secure connection for communicating the message.
21. The system of claim 15, the one or more processors further operable to:
determine that the request fails to correspond to the designated link provided to the intended recipient; and
initiate a fallback authentication procedure.

1. A method for creating a self-replicating synthetic cell, said method comprising:
(i) assembling a synthetic bacterial, cyanobacterial, or microalgal donor genome as one or more fragments and introducing the donor genome as one or more fragments and a host vector into a yeast host cell, wherein the donor genome and the host vector are joined prior to or after introduction into the yeast host cell;
(ii) recovering the assembled donor genome from the yeast host cell;
(iii) performing step a) or b) or c) wherein a), b) and c) comprise
a) preparing the donor genome for transplantation into a bacterial, cyanobacterial, or microalgal recipient cell by methylating the donor genome;
b) preparing a bacterial or cyanobacterial or microalgal recipient cell by removing or inactivating a restriction endonuclease function present in the recipient cell that cuts the donor genome;
c) providing a bacterial, cyanobacterial, or microalgal recipient cell lacking a restriction endonuclease function that cuts the donor genome; and

(iv) introducing the recovered donor genome into the recipient cell,
thereby generating a self-replicating synthetic cell comprising the donor genome and controlled only by the donor genome, wherein the donor genome is sufficient to sustain viability and continuous self-replication of the recipient cell; and
wherein the synthetic cell supports gene expression from the donor genome and has a phenotype of the donor genome,
wherein the donor genome is an essentially intact genome that is at least a minimal genome, and is greater than about 300 kb in length.
2. The method of claim 1, wherein the donor genome and the host vector are introduced into the host cell simultaneously.
3. The method of claim 1, wherein the donor genome and host vector are joined prior to introduction into the yeast host cell by transforming the yeast host vector into a donor cell containing the donor genome.
4. The method of claim 3, wherein the host vector is a centromeric plasmid.
5. The method of claim 1, wherein the donor genome is modified within the yeast host cell.
6. The method of claim 1, further comprising degrading or removing the endogenous genome of the recipient cell.
7. The method of claim 1, wherein the recovered donor genome is methylated prior to introduction into the recipient cell.
8. The method of claim 1, wherein the recipient cell’s restriction endonuclease function is absent, removed or inactivated.
9. The method of claim 1, further comprising introducing a second donor genome into the host cell, wherein the second donor genome is different from the first donor genome, thereby producing a host cell containing two different donor genomes.
10. The method of claim 9, wherein introducing the second donor genome comprises mating the host cell containing the first donor genome with a second host cell containing the second donor genome.
11. The method of claim 1, wherein the synthetic cell exhibits a phenotype corresponding to the donor genome incorporating any modifications thereto.
12. The method of claim 1 wherein the synthetic donor genome is assembled in vitro prior to introducing the donor genome into the host cell.
13. The method of claim 5 wherein the modification of the donor genome is selected from the group consisting of a substitution, a deletion, an insertion, a rearrangement, and a recombination.
14. The method of claim 13 wherein the modification is selected from the group consisting of: an insertion, a deletion, and a substitution.
15. The method of claim 5 wherein the modification is a homologous recombination.
16. The method of claim 1 wherein the donor genome is a bacterial genome and the recipient cell is a bacterial cell.
17. The method of claim 1 wherein the donor genome is a cyanobacterial genome and the recipient cell is a cyanobacterial cell.
18. The method of claim 1 wherein the donor genome is a microalgal genome and the recipient cell is a microalgal cell.
19. The method of claim 1 wherein the yeast host cell is Saccharomyces cerevisiae or Saccharomyces pombe.
20. The method of claim 16, wherein the donor genome is a Mycoplasma genome and the recipient cell is a Mycoplasma cell.
21. The method of claim 19, wherein the recipient cell is a Mycoplasma capricolum.
22. The method of claim 20, wherein the recipient cell is a Mycoplasma capricolum.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A storage system comprising:
a first disk array system, in a primary site, coupled to a host computer, and having a plurality of first disk drive units in which data are stored and a first controller which controls to store data sent from said host computer to a primary volume related to a portion of said first disk drive units;
a second disk array system, in a remote site, coupled to said first disk array system, and having a plurality of second disk drive units in which data are stored and a second controller which receives data of said primary volume from said first disk array system by an asynchronous remote copy procedure and controls to store data received from said first disk array system to a secondary volume related to a portion of said second disk drive units; and
a third disk array system, coupled to said first disk array system, and having a plurality of third disk drive units in which data are stored and a third controller which receives data of said primary volume from said first disk array system by a synchronous copy procedure and controls to store data received from said first disk array system to a third volume related to a portion of said third disk drive units;
wherein said third disk array system comprises a first area and a second area, said first area storing information related to data of a plurality of write requests received from said first disk array system by said synchronous copy procedure during a first time period,
wherein said second area storing information related to data of a plurality of write requests received from said first disk array system by said synchronous copy procedure during a second time period being different from said first time period,
wherein said third disk array system stores information related to data of a plurality of write requests received from said first disk array system in said first area during said first time period and said second area during said second time period, and
wherein said information stored in said second area is used to decide data to be transferred from said third disk array system to said second disk array system in the event of a disaster that affects said primary site.