1. A titanium alloy composite, comprising:
a first metal part made of a titanium alloy and having micron-scale roughness produced by chemical etching, the surface thereof having, under electron microscopy at 100,000 magnifications, ultra-fine irregularities in which mountain- or mountain-range shaped projections having a height and width of 10 to 350 nm and a length of 10 nm or more are present over the entire surface at a period of 10 to 350 nm, said surface being mainly a thin layer of a titanium oxide; and
another adherend that is bonded using, as an adhesive, an epoxy adhesive that penetrates into the ultra-fine irregular surface.
2. A titanium alloy composite, comprising:
a first metal part made of \u03b1-\u03b2 titanium alloy and having a roughness-curve average length (RSm) of 1 to 10 \u03bcm and a maximum height roughness (Rz) of 1 to 5 \u03bcm under scanning probe microscopy, the surface thereof having fine irregularities in which both smooth dome shapes and dead leaf-like shapes are observed within a 10 \u03bcm square area under electron microscopy at 10,000 magnifications, said surface being mainly a thin layer of a metal oxide comprising titanium and aluminum; and
another adherend that is bonded using, as an adhesive, an epoxy adhesive that penetrates into the surface.
3. The titanium alloy composite according to claim 1 or 2,
wherein said adherend is a second metal part made of a titanium alloy having said ultra-fine irregular surface formed thereon.
4. The titanium alloy composite according to claim 1 or 2,
wherein said adherend is a fiber-reinforced plastic, comprising said epoxy adhesive, and reinforced through filling and laminating of one or more types selected from among long fibers, short fibers and fiber cloth.
5. The titanium alloy composite according to claim 1,
wherein said micron-scale surface roughness has an average length (RSm) of 0.8 to 10 \u03bcm and a maximum height roughness (Rz) of 0.2 to 5 \u03bcm.
6. The titanium alloy composite according to claim 1 or 2,
wherein said chemical etching involves immersion in a strongly acidic aqueous solution containing a hydrogen fluoride compound.
7. The titanium alloy composite according to claim 1 or 2,
wherein said chemical etching involves immersion in an aqueous solution of ammonium bifluoride.
8. The titanium alloy composite according to claim 1 or 2,
wherein a resin fraction of a cured product of said epoxy adhesive comprises no more than 30 parts by weight of an elastomer component relative to a total 100 parts by weight of resin fraction.
9. The titanium alloy composite according to claim 1 or 2,
wherein a cured product of said epoxy adhesive contains a total of no more than 100 parts by weight of a filler relative to a total 100 parts by weight of resin fraction.
10. The titanium alloy composite according to claim 9,
wherein said filler is one or more types of reinforcing fiber selected from among glass fibers, carbon fibers and aramid fibers, or
one or more types of a powder filler selected from among calcium carbonate, magnesium carbonate, silica, talc, clay and glass.
11. The titanium alloy composite according to claim 8,
wherein said elastomer component has a particle size of 1 to 15 \u03bcm, and is one or more types selected from among vulcanized rubber powder, semi-crosslinked rubber, unvulcanized rubber, a terminal-modified thermoplastic resin of a hydroxyl group-terminated polyether sulfone having a melting pointsoftening point not lower than 300\xb0 C., and a polyolefin resin.
12. A method for manufacturing a titanium alloy composite, comprising:
a shaping step of mechanically shaping a titanium alloy part from a casting or an intermediate material;
a chemical etching step of immersing said titanium alloy part in an aqueous solution comprising ammonium bifluoride;
a coating step of coating an epoxy adhesive on required portions of said titanium alloy part;
a shape-adjustment step of adjusting a prepreg material of fiber-reinforced plastic to the required size;
an affixing step of affixing said prepreg material of fiber-reinforced plastic to the coated surface of the titanium alloy part; and
a curing step of curing an entire epoxy resin fraction by positioning, pressing and heating said prepreg material and said titanium alloy part.
13. A method for manufacturing a titanium alloy composite, comprising:
a shaping step of mechanically shaping a titanium alloy part from a casting or an intermediate material;
a chemical etching step of immersing said titanium alloy part in an aqueous solution comprising ammonium bifluoride, to form ultra-fine irregularities;
a coating step of coating an epoxy adhesive on required portions of said titanium alloy part;
a curing pre-treatment step of placing the titanium alloy part, having been coated with said epoxy adhesive, in an airtight vessel, depressurizing the vessel, and then pressurizing the vessel to thereby push the epoxy adhesive into said ultra-fine irregularities on the surface of the titanium alloy;
a shape-adjustment step of adjusting a prepreg material of fiber-reinforced plastic to the required size;
an affixing step of affixing said prepreg material to the coated surface of said titanium alloy part; and
a curing step of curing an entire epoxy resin fraction by positioning, pressing and heating said prepreg material and said titanium alloy part.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A method for obtaining a stem cell-like cell from a sample taken from a multicellular organism, said method comprising:
culturing cells from said sample and allowing for transcription, translation or expression by at least one of said cells of a gene or fragment thereof that, in general, is differentially expressed at different phases of embryonic development, and obtaining a stem cell-like cell.
2. The method according to claim 1 wherein said organism is functionally differentiated.
3. The method according to claim 1 or claim 2 wherein said organism is a vertebrate.
4. The method according to any one of claims 1 to 3 further comprising culturing cells from said sample in the relative absence of a differentiation factor
5. The method according to claim 4 wherein said differentiation factor has retinoid activity.
6. The method according to any one of claims 1 to 5 wherein said gene is overexpressed in an early phase of embryonic development.
7. The method according to claim 6 wherein said early phase comprises the blastula stage.
8. The method according to claim 7 wherein, in mammals, said blastula stage comprises a pre-implantation stage.
9. The method according to any one of claims 1 to 8 wherein said gene comprises Oct4 or orthologue thereof.
10. The method according to any one of claims 1-9 further comprising:
selecting said stem cell-like cell by detecting expression of cell surface markers stage specific embryonic antigen.
11. The method according to claim 10 wherein said cell surface markers stage specific embryonic antigen comprises SSEA-1, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 andor alkaline phosphatase or analogue thereof.
12. A cell wherein said cell is a dedifferentiated stem cell.
13. A cell wherein said cell is a stem cell-like cell produced by a method according to any one of claims 1-11.
14. The cell of claim 12 or claim 13 comprising a recombinant nucleic acid.
15. A culture comprising the cell of claim 12, claim 13, or claim 14.
16. A graft or transplantation material comprising the cell of claim 12, claim 13, or claim 14 or the culture of claim 15.
17. A non-human animal comprising the cell of claim 12, claim 13 or claim 14 or the culture of claim 15.
18. A pharmaceutical composition for treating a subject with a graft, said pharmaceutical composition comprising the cell of claim 12, claim 13, or claim 14 or the culture of claim 15.
19. The pharmaceutical composition of claim 18 wherein the subject or a sample taken therefrom comprises a source of the graft.
20. A method of cloning a non-human animal, the improvement comprising using in said method the cell of claim 12, claim 13, or claim 14 or the culture of claim 15 in the cloning of the non-human animal.
21. The according to claim 20 wherein said non-human animal is an experimental animal, a farm animal, or an animal for xenotransplant production.