Author: claimsbot

1. A microorganism concentrating apparatus comprising:
a mixing device configured to mix an aqueous suspension of microorganisms with a particulate filter aid comprising magnetic particles to prepare an aqueous slurry of the microorganisms and the particulate filter aid;
a filtering device configured to filter the aqueous slurry supplied from the mixing device to provide a filter cake comprising the microorganisms and the particulate filter aid, and a filtrate; and
a magnetic separation device configured to magnetically separate the filter cake supplied from the filtering device into the microorganisms and the particulate filter aid.
2. The apparatus according to claim 1, wherein the particulate filter aid has an average particle size 0.1 to 10 times that of the microorganisms.
3. The apparatus according to claim 2, wherein the particulate filter aid consists of the magnetic particles.
4. The apparatus according to claim 2, wherein the particulate filter aid consists of aggregates of magnetic particles whose surfaces are covered with a polymer.
5. The apparatus according to claim 1, wherein the filtering device comprises an envelope and a horizontally arranged filter medium partitioning the inner space of the envelope into an upper space into which the aqueous slurry is supplied and a lower space from which the filtrate is discharged, wherein the filter cake is formed on the filter medium.
6. The apparatus according to claim 1, wherein the magnetic separation device comprises a tank which receives the filter cake supplied from the filtering device, and an electromagnet arranged on the outer wall of the tank.
7. The apparatus according to claim 5, further comprising an aqueous wash-out liquid supply line configured to supply an aqueous wash-out liquid to the filter cake, thereby washing out the filter cake from the filter medium.
8. The apparatus according to claim 7, further comprising a filter cake supply line supplying the washed-out filter cake together with the aqueous wash-out liquid to the magnetic separation device.
9. The apparatus according to claim 1, wherein the magnetic particles are formed of magnetite.
10. The apparatus according to claim 1, wherein the microorganisms comprises E. coli, waterbloom or chlorella.
11. A microorganism concentrating method comprising:
mixing an aqueous suspension of microorganisms with a particulate filter aid comprising magnetic particles to prepare an aqueous slurry of the microorganisms and the particulate filter aid;
filtering the aqueous slurry through a filter medium to provide a filter cake comprising the microorganisms and the particulate filter aid, and a filtrate, wherein the filter cake is provided on the filter medium; and
magnetically separating the filter cake into the microorganisms and the particulate filter aid.
12. The method according to claim 11, wherein an aqueous wash-out liquid is flowed toward the filter cake to wash out the filter cake from the filter medium, thereby preparing an aqueous slurry comprising the washed-out filter cake, which is subjected to the magnetic separation.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An electrospray ion source for a mass spectrometer comprising:
a source of an analyte-bearing liquid;
a source of a sheath gas;
a plurality of liquid conduits, each liquid conduit configured so as to receive a portion of the analyte-bearing liquid from the source of analyte-bearing liquid;
at least one electrode for producing electrospray emission of charged droplets from an outlet of each of said liquid conduits under application of an electrical potential to the at least one electrode;
a power supply electrically coupled to the at least one electrode for maintaining the at least one electrode at the electrical potential; and
a plurality of sheath gas conduits, each sheath gas conduit comprising:
an inlet configured to receive a sheath gas portion from the source of sheath gas; and
an outlet configured to emit a sheath gas flow that circumferentially surrounds, in at least two dimensions, a portion of the charged droplets emitted from a respective one of the liquid conduit outlets.
2. An electrospray ion source as in claim 1, wherein each sheath gas conduit comprises a tube that at least partially encloses a respective one of the liquid conduits.
3. An electrospray ion source as in claim 1, wherein each liquid conduit comprises a capillary.
4. An electrospray ion source as in claim 1, wherein the at least one electrode comprises a plurality of electrodes, each electrode of the plurality of electrodes associated with a respective one of the liquid conduits for producing the electrospray emission of the charged droplets from the outlet of said respective one of the liquid conduits.
5. An electrospray ion source as in claim 4, wherein each liquid conduit is the respective electrode associated with the liquid conduit.
6. An electrospray ion source as in claim 1, further comprising:
a block though which the plurality of liquid conduits passes,
wherein each sheath gas conduit comprises a channel in the block, the channel at least partially enclosing a respective one of the liquid conduits.
7. An electrospray ion source as in claim 6, wherein at least two of the channels are angled with respect to one another so that the respective emitted sheath gas flows provide spatial confinement of a portion of the charged droplets emitted from the respective liquid conduit outlets.
8. An electrospray ion source as in claim 1, further comprising at least one additional electrode configured so as to improve uniformity of emission of charged droplets across the plurality of liquid conduit outlets.
9. An electrospray ion source as in claim 8, wherein the at least one additional electrode comprises a plurality of additional electrodes.
10. An electrospray ion source as in claim 1, further comprising at least one heater associated with the plurality of sheath gas conduits so as to heat the sheath gas portions.
11. An electrospray ion source as in claim 1, wherein each sheath gas conduit comprises a cross sectional area associated with the sheath gas flow therein, wherein the plurality of said cross sectional areas are substantially identical to one another.
12. An electrospray ion source as in claim 1, wherein each liquid conduit is configured so as to admit a flow rate of the analyte-bearing liquid portion of between 1 microliter per minute and 1 milliliter per minute.
13. An electrospray ion source for a mass spectrometer comprising:
a source of an analyte-bearing liquid;
a source of a sheath gas;
a plurality of liquid conduits, each liquid conduit configured so as to receive a portion of the analyte-bearing liquid from the source of analyte-bearing liquid;

at least one electrode for producing electrospray emission of charged droplets from an outlet of each of said liquid conduits under application of an electrical potential to the at least one electrode;
a power supply electrically coupled to the at least one electrode for maintaining the at least one electrode at the electrical potential; and
a sheath gas conduit comprising:
an inlet configured to receive the sheath gas from the source of sheath gas; and
an outlet configured to emit a sheath gas flow that circumferentially surrounds, in at least two dimensions, a portion of the charged droplets emitted from every one of the plurality of liquid conduit outlets.
14. An electrospray ion source as in claim 13, wherein the sheath gas conduit comprises a groove in a block that at least partially encloses the plurality of liquid conduits.
15. An electrospray ion source as in claim 13, wherein at least a portion of the sheath gas conduit is disposed at angle with respect to the plurality of liquid conduits so that the emitted sheath gas flow provides spatial confinement of a portion of the charged droplets emitted from the plurality of liquid conduit outlets.
16. An electrospray ion source as in claim 13, wherein the sheath gas conduit comprises a tube that at least partially encloses every one of the plurality of liquid conduits.
17. An electrospray ion source as in claim 13, wherein each liquid conduit comprises a capillary.
18. An electrospray ion source as in claim 13, wherein the at least one electrode comprises a plurality of electrodes, each electrode of the plurality of electrodes associated with a respective one of the liquid conduits for producing the electrospray emission of the charged droplets from the outlet of said respective one of the liquid conduits.
19. An electrospray ion source as in claim 18, wherein each liquid conduit is the respective electrode associated with the liquid conduit.
20. An electrospray ion source as in claim 13, further comprising at least one additional electrode configured so as to improve uniformity of emission of charged droplets across the plurality of liquid conduit outlets.
21. An electrospray ion source as in claim 20, wherein the at least one additional electrode comprises a plurality of additional electrodes.
22. An electrospray ion source as in claim 13, further comprising at least one heater associated with sheath gas conduit so as to heat the sheath gas.
23. An electrospray ion source as in claim 13, wherein each liquid conduit is configured so as to admit a flow rate of the analyte-bearing liquid portion of between 1 microliter per minute and 1 milliliter per minute.
24. A method for providing ions to a mass spectrometer, comprising:
providing a source of an analyte-bearing liquid;
providing a source of a sheath gas;
providing a plurality of liquid conduits, each liquid conduit configured so as to receive a portion of the analyte-bearing liquid from the source of analyte-bearing liquid;
providing at least one electrode associated with the plurality of liquid conduits;
providing a plurality of sheath gas conduits, each sheath gas conduit comprising a sheath gas outlet configured to emit a sheath gas flow that circumferentially surrounds, in at least two dimensions, an outlet of a respective one of the liquid conduits;
distributing the analyte-bearing liquid among the plurality of liquid conduits;
distributing the sheath gas among the plurality of sheath gas conduits; and
maintaining the at least one electrode at an electrical potential such that charged liquid droplets are emitted from the plurality of liquid conduits.
25. A method for providing ions to a mass spectrometer as in claim 24, wherein the step of providing a plurality of sheath gas conduits comprises providing a plurality of tubes, each tube at least partially enclosing the respective liquid conduit.
26. A method for providing ions to a mass spectrometer as in claim 24, wherein the step of providing a plurality of sheath gas conduits comprises providing a plurality of channels in a block, the block at least partially enclosing the plurality of liquid conduits.
27. A method for providing ions to a mass spectrometer as in claim 24, further comprising providing at least one heater associated with the plurality of sheath gas conduits so as to heat the sheath gas.
28. A method for providing ions to a mass spectrometer as in claim 24, further comprising providing a heated auxiliary gas encompassing said charged liquid droplets.
29. A method for providing ions to a mass spectrometer, comprising:
providing a source of an analyte-bearing liquid;
providing a source of a sheath gas;
providing a plurality of liquid conduits, each liquid conduit configured so as to receive a portion of the analyte-bearing liquid from the source of analyte-bearing liquid and having a respective outlet;
providing at least one electrode associated with the plurality of liquid conduits;
providing a sheath gas conduit comprising a sheath gas outlet configured to emit a sheath gas flow that circumferentially surrounds, in at least two dimensions, the outlets of the plurality of liquid conduit outlets;
distributing the analyte-bearing liquid among the plurality of liquid conduits;
providing the sheath gas to the sheath gas conduit; and
maintaining the at least one electrode at an electrical potential such that charged liquid droplets are emitted from the plurality of liquid conduits.
30. A method for providing ions to a mass spectrometer as in claim 29, wherein the step of providing a sheath gas conduit comprises providing a sheath gas conduit that at least partially encloses the plurality of liquid conduits.
31. A method for providing ions to a mass spectrometer as in claim 29, wherein the step of providing a sheath gas conduit comprises providing a groove in a block, the block at least partially enclosing the plurality of liquid conduits.
32. A method for providing ions to a mass spectrometer as in claim 29, further comprising providing at least one heater associated with the sheath gas conduits so as to heat the sheath gas.
33. A method for providing ions to a mass spectrometer as in claim 29, further comprising providing a heated auxiliary gas encompassing said charged liquid droplets.

1. A method for generating a control voltage for a position actuator of a disk drive system for displacing a lens of a pick-up unit to a given position, wherein the actuator is operated by an open loop control system, said method comprising:
calibration of the control system for producing only a single control voltage (Va) from an input position signal (Xact_setp) to the control system;
processing only said single control voltage (Va) in a feed forward system for generating only a single processed control signal (Va_p), wherein said processing of only said single control voltage (Va) is performed as a function of the present axial (Z) position of the actuator, the present temperature, the (Z) position of the actuator during calibration, and the temperature during calibration,
feeding only said single processed control signal to said position actuator for displacing said lens to said given position
wherein said calibration step calibrates at least one of parameters K, R and C,
where K=property of \u2018actuator\u2019: NewtonAmpere
R=resistance of \u2018actuator\u2019 coil
C=Spring constant in Newtonmeter.
2. The method as claimed in claim 1, further comprising measuring a property dependent on an axial distance of the lens of the pick-up unit from a calibrated axial position, a Z-value, for use by said feed forward system and a temperature of the pick-up unit are measured.
3. The method as claimed in claim 1, further comprising measuring a property dependent on a temperature of the pick-up unit for use by said feed forward system.
4. The method as claimed in claim 1, in which said processed control signal is processed in dependence of an estimated distance of the lens from a calibrated axial position andor an estimated temperature of the pick-up unit.
5. The method as claimed in claim 1, in which said position actuator operates according to the formula:
Xdc=Va\u2014p*K(Temp,Z)(R(Temp)*C(Temp,Z))
in which
Xdc=position signal for said given position
Va_p=a processed control voltage
K=property of \u2018actuator\u2019 21: NewtonAmpere
R=resistance of \u2018actuator\u2019 coil
C=Spring constant in Newtonmeter.
6. The method as claimed in claim 5, in which the dependencies of temperature and Z-value of said parameters are estimated, and said processing step involves compensation of the dependency of said parameters by adjusting said control voltage to said processed control voltage.
7. The method as claimed in claim 1, wherein said processed voltage is calculated according to the formula:
Va\u2014p=Xact\u2014setp*Cal*S(Temp_cal,Z_cal)S(Temp_write,Z_write)
in which
Cal is the result of the calibration
S(Temp,Z)=(A\u2014T+B\u2014T*Temp+C\u2014T*Temp^2)*(A\u2014Z+B\u2014Z*Vz+C\u2014Z*Vz^2)
wherein
A_T, B_T, C_T, A_Z, B_Z, C_Z are constants;
Vz is the voltage across the Z actuator,
Temp_cal and Vz_cal are the temperature and Vz, respectively, during calibration,
Temp_write and Vz_write are the temperature and Vz, respectively, during writing.
8. A device for carrying out the method as claimed in claim 1, for generating a control voltage for a position actuator of a disk drive system for displacing a lens of a pick-up unit to a given position, wherein the actuator is operated by an open loop control system, said device comprising:
a calibration means for calibration of the control system for producing only a single control voltage (Va) from an input position signal (Xact_setp) to the control system;
a processing means for processing said only single control voltage in a feed forward system for generating only a single processed control signal (Va_p);
a position actuator for receiving said processed only single control signal and for displacing said lens to said given position
wherein said calibration means calibrates at least one of parameters K, R and C,
where K=property of \u2018actuator\u2019: NewtonAmpere
R=resistance of \u2018actuator\u2019 coil
C=Spring constant in Newtonmeter.
9. The device as claimed in claim 8, further comprising a temperature sensor.
10. The device as claimed in claim 8, further comprising a means for estimating the axial distance of the pick-up unit from a calibrated axial position, a Z-value, for example by means of an input of the actual actuator driver or by means of its output.
11. A method for generating a control voltage for a position actuator of a disk drive system for displacing a lens of a pick-up unit to a given position, wherein the actuator is operated by an open loop control system, said method comprising:
calibration of the control system for producing a control voltage (Va) from an input position signal (Xact_setp) to the control system;
processing said control voltage in a feed forward system for generating a processed control signal (Va_p);
feeding said processed control signal to said position actuator for displacing said lens to said given position
wherein said position actuator operates according to the formula:
Xdc=Va\u2014p*K(Temp,Z)(R(Temp)*C(Temp,Z))
in which
Xdc=position signal for said given position
Va_p=Va processed
K=property of \u2018actuator\u2019 21: NewtonAmpere
R=resistance of \u2018actuator\u2019 coil
C=Spring constant in Newtonmeter.
12. The method as claimed in claim 11, in which said calibration step calibrates at least one of said parameters K, R, C.
13. The method as claimed in claim 11, in which the dependencies of temperature and Z-value of said parameters are estimated, and said processing step involves compensation of the dependency of said parameters by adjusting said control voltage to said processed control voltage.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A pharmaceutical composition comprising:
an indolocarbazole compound present in a solubilized concentration ranging from about 3% up to about 9% by weight of the total composition; a hydrophilic polymer component; and water; wherein said composition is a microemulsion.
2. The composition according to claim 1, wherein said indolocarbazole is lestaurtinib or 9S-(9\u03b1, 10\u03b2,12\u03b1)-2,3,9,10,11,12-hexahydro-10-hydroxy-10-(hydroxymethyl)-9-methyl-9,12-epoxy-1H-diindolo1,2,3-fg:3\u2032,2\u2032,1\u2032-klpyrrolo3,4-i1,6benzodiazocin-1-one.
3. The composition according to claim 1, wherein said indolocarbazole compound is lestaurtinib and said lestaurtinib is present in a solubilized concentration of between about 3% and about 9% of the total composition weight of the microemulsion.
4. The composition according to claim 1, wherein said hydrophilic polymer component comprises polyethylene glycol.
5. The composition according to claim 1, wherein said composition further comprises a surfactant.
6. The composition according to claim 5, wherein said surfactant is selected from the group consisting of anionic surfactants, cationic surfactants, and nonionic surfactants, and combinations thereof.
7. The composition according to claim 6, wherein said surfactant is a polyoxyethyl stearate.
8. The composition according to claim 1, further comprising an antioxidant component selected from the group consisting of H-atom donor antioxidant, sacrificial antioxidants and oxygen scavenger antioxidants, and combinations thereof.
9. The composition according to claim 8, wherein said antioxidant is selected from the group consisting of vitamin E, ascorbic acid, KMBS (potassium metabisulfite salt), ascorbyl palmitate, sodium ascorbate, BHA (butylated hydroxyanisole), BHT (butlyated hydroxytoluene) and combinations thereof.
10. An oral capsular dosage form comprising a pharmaceutical fill composition encapsulated within a capsule material, said composition comprising:
a) indolocarbazole compound present in an amount ranging from about 3% to about 9% per total composition weight;
b) a hydrophilic polymer component present in an amount ranging from about 30% to about 95% per total composition weight; and
c) water present in an amount ranging from about 0.8% to about 50% by weight of total composition weight;

wherein said composition is a microemulsion formulated for encapsulation with a hard capsule material.
11. The dosage form according to claim 10, wherein said capsule material is a hard capsule material selected from the group consisting of gelatin and hydroxypropylmethylcellulose.
12. The dosage form according to claim 10, wherein said indolocarbazole compound is lestaurtinib.
13. The dosage form according to claim 12, wherein said composition comprises:
a) lestaurtinib present in an amount from about 3% to about 9% total composition weight;
b) hydrophilic polymer component comprising a polyethylene glycol present in an amount from about 30% to about 90% total composition weight;
c) a surfactant comprising a polyoxyl stearate and present in an amount from about 5% to about 45% total composition weight;
d) water present in an amount from about 0.8% to about 15% total composition weight; and
e) an antioxidant component comprising a mixture of antioxidants and present in an amount from about 0.1% to about 2% total composition weight;

wherein said composition is in the form of a microemulsion and encapsulated within a hard capsule.
14. The dosage form according to claim 13, where said composition comprises:
a) lestaurtinib present in an amount from about 5% to about 7% total composition weight;
b) PEG-1000 present in an amount from about 35% to about 45% total composition weight;
c) MYRJ\xae 52 present in an amount from about 35% to about 45% total composition weight;
d) water present in an amount from about 6% to about 8% total composition weight;
e) vitamin E present in an amount which is about 0.075% total composition weight;
f) ascorbyl palmitate present in an amount which is about 0.1% total composition weight;
g) ascorbic acid present in an amount which is about 0.1% total composition weight; and
h) KMBS present in an amount which is about 0.2% total composition weight;
wherein said composition is in the form of a microemulsion and encapsulated within a hard capsule.
15. The dosage form according to claim 13, where said composition comprises:
a) lestaurtinib present in an amount from about 5% to about 7% total composition weight;
b) PEG-1000 present in an amount from about 35% to about 45% total composition weight;
c) MYRJ\xae 52 present in an amount from about 35% to about 45% total composition weight;
d) water present in an amount from about 6% to about 8% total composition weight;
e) vitamin E present in an amount which is about 0.075% total composition weight;
f) ascorbyl palmitate present in an amount which is about 0.1% total composition weight;
g) sodium ascorbate present in an amount which is about 0.125% total composition weight; and
h) KMBS present in an amount which is about 0.2% total composition weight;

wherein said composition is in the form of a microemulsion and encapsulated within a hard capsule.
16. The dosage form according to claim 13, where said composition comprises:
a) lestaurtinib present in an amount from about 5% to about 7% total composition weight;
b) PEG-1000 present in an amount from about 35% to about 45% total composition weight;
c) MYRJ\xae 52 present in an amount from about 35% to about 45% total composition weight;
d) water present in an amount from about 6% to about 8% total composition weight;
e) vitamin E present in an amount which is about 0.15% total composition weight;
f) ascorbyl palmitate present in an amount which is about 0.2% total composition weight; and
g) ascorbic acid present in an amount which is about 0.05% total composition weight;

wherein said composition is in the form of a microemulsion and encapsulated within a hard capsule.
17. The dosage form according to claim 13, where said composition comprises:
a) lestaurtinib present in an amount from about 5% to about 7% total composition weight;
b) PEG-1000 present in an amount from about 35% to about 45% total composition weight;
c) MYRJ\xae 52 present in an amount from about 35% to about 45% total composition weight;
d) water present in an amount from about 6% to about 8% total composition weight;
e) vitamin E present in an amount which is about 0.15% total composition weight; and
f) ascorbyl palmitate present in an amount which is about 0.2% total composition weight;

wherein said composition is in the form of a microemulsion and encapsulated within a hard capsule.
18. A pharmaceutical composition comprising a microemulsion having an indolocarbazole compound as an active ingredient present in a solubilized concentration ranging from about 3% up to about 9% of the total composition weight, said composition being prepared by:
a) combining an indolocarbazole compound, hydrophilic polymer component, and surfactant;
b) adding water to the combined ingredients from step a) in an amount sufficient to increase the solubilized concentration of said indolocarbazole compound to the desired concentration amount;

wherein steps a) and b) are performed at a temperature sufficient to form a molten liquid of the combined ingredients, facilitate solubilization of said indolocarbazole compound and form a microemulsion.
19. A process for increasing the solubilized concentration of an indolocarbazole compound in a microemulsion for a given fill volume, said process comprising:
a) combining an indolocarbazole compound, hydrophilic polymer component, and surfactant;
b) adding water to the combined ingredients from step a) in an amount sufficient to increase the solubilized concentration of said indolocarbazole compound to the desired concentration amount;

wherein steps a) and b) are performed at a temperature sufficient to form a molten liquid of the combined ingredients, facilitate solubilization of said indolocarbazole compound and form a microemulsion;
wherein said indolocarbazole is present in an amount of up to about 9% total composition weight.
20. A method of inhibiting receptor-tyrosine kinase in a recipient comprising orally administering to a recipient in need of such treatment an oral dosage form having a pharmaceutical composition comprising:
a) lestaurtinib present in a solubilized concentration of from about 3% to about 9% by weight of the total composition;
b) a hydrophilic polymer component comprising polyethylene glycol;
c) a surfactant; and
d) water;

wherein said composition is a microemulsion.
21. An oral tablet dosage form comprising a pharmaceutical composition, said composition comprising:
a) indolocarbazole compound present in an amount ranging from about 3% to about 9% per total composition weight;
b) a hydrophilic polymer component;
c) water; and
d) additional pharmaceutically acceptable excipients;

wherein said composition is a microemulsion formulated for compression into a tablet dosage form.
22. The dosage form according to claim 21, wherein said composition comprises:
a) lestaurtinib present in an amount which is about 3% total composition weight;
b) PEG-1000 present in an amount which is about 17% total composition weight;
c) water present in an amount which is about 4% total composition weight; and
d) additional pharmaceutically acceptable excipients selected from the group consisting of magnesium aluminometasilicate, microcrystalline cellulose, lactose, sodium starch glycolate and magnesium stearate;

wherein said composition is a microemulsion formulated for compression into a tablet dosage form.