1461126032-8599b29c-d8a9-4dad-ab60-975d81937c4c

What is claimed is:

1. In a bus bar holder having at least one bus bar receiver (1.3) formed in a support element (1), and a variable width for receiving bus bars (2) of different cross sections with an adapter element (3), which can be plugged or locked in a holding structure (1.11) with a matched and complementary holding structure (3.1) in a bottom area, the improvement comprising:
a separate spacer (5) for adapting the at least one bus bar receiver (1.3) in a depth direction, the spacer (5) removably attached on the bottom area (1.32) of the bus bar receiver (1.3), the spacer (5) having a free space (5.4) in an area of a holding structure (1.11), and the holding structure (1.11) being accessible through the free space (5.4) for inserting the adapter element (3).
2. In the bus bar holder in accordance with claim 1, wherein on an underside facing the bottom area (1.32) the spacer (5) has at least one protrusion (5.3), and the bottom area (1.32) having at least one hole (1.34) for the plug-in or locked-in fastening of the at least one protrusion (5.3).
3. In the bus bar holder in accordance with claim 2, wherein the spacer (5) has one of a U-shaped and a rectangular outer contour.
4. In the bus bar holder in accordance with claim 3, wherein the free space (5.4) is rectangular, and the at least one protrusion (5.3) is arranged under at least one transverse strip of the spacer (5).
5. In the bus bar holder in accordance with claim 4, wherein the holding structure (1.11) is formed in a recess (1.1) in the bottom area (1.32).
6. In the bus bar holder in accordance with claim 5, wherein the holding structures (1.11) are cut at the inner wall sides which border the recess (1.1) in a longitudinal direction of one of the bus bars (2) to be inserted, and the spacer (5) rests with lateral areas (5.1) on the corresponding wall areas.
7. In the bus bar holder in accordance with claim 6, wherein a complementary structure (3.1) is formed on two narrow long sides of the adapter element (3), which is flat in a front view.
8. In the bus bar holder in accordance with claim 7, wherein at a bottom of the recess (11.1) there is at least one cutout (1.2) in which the complementary structure (3.1) engages with additional structure sections (3.11).
9. In the bus bar holder in accordance with claim 8, wherein for fixing the bus bar in place (2), first bores (1.5) for attaching a holding device, and second bores (1.4, 1.6) for mounting the support element (1) on a base, are cut into a top of the support element (1).
10. In the bus bar holder in accordance with claim 9, wherein the holding device is formed as a one-piece cover (4) which extends over each said bus bar receiver (1.3) and can be one of screwed and locked onto the support element (1).
11. In the bus bar holder in accordance with claim 10, wherein on an underside facing the support element (1) the cover (4) has hollow spaces (4.2) which are matched to the respective head pieces (3.3) of the adapter elements (3) for receiving in an inserted state and are arranged corresponding to various insertion positions of the adapter elements (3) in the support element (1).
12. In the bus bar holder in accordance with claim 11, wherein one of there is a hollow space (4.2) with holding structures for each said bus bar receiver (1.3) in the cover (4), and there are several individual hollow spaces (4.2) corresponding to the various insertion positions of the adapter elements (3).
13. In the bus bar holder in accordance with claim 12, wherein protrusions (4.5) for pressing each said bus bar (2) against the cover (4) are provided on the underside of the cover (4).
14. In the bus bar holder in accordance with claim 1, wherein the spacer (5) has one of a U-shaped and a rectangular outer contour.
15. In the bus bar holder in accordance with claim 14, wherein the free space (5.4) is rectangular, and at least one protrusion (5.3) is arranged under at least one transverse strip of the spacer (5).
16. In the bus bar holder in accordance with claim 3, wherein the holding structure (1.11) is formed in a recess (1.1) in the bottom area (1.32).
17. In the bus bar holder in accordance with claim 16, wherein the holding structures (1.11) are cut at inner wall sides which border the recess (1.1) in a longitudinal direction of one of the bus bars (2) to be inserted, and the spacer (5) rests with lateral areas (5.1) on the corresponding wall areas.
18. In the bus bar holder in accordance with claim 1, wherein a complementary structure (3.1) is formed on two narrow long sides of the adapter element (3), which is flat in a front view.
19. In the bus bar holder in accordance with claim 5, wherein at a bottom of the recess (1.1) there is at least one cutout (1.2) in which a complementary structure (3.1) engages with additional structure sections (3.11).
20. In the bus bar holder in accordance with claim 1, wherein for fixing the bus bar in place (2), first bores (1.5) for attaching a holding device, and second bores (1.4, 1.6) for mounting the support element (1) on a base, are cut into a top of the support element (1).
21. In the bus bar holder in accordance with claim 20, wherein the holding device is formed as a one-piece cover (4) which extends over each said bus bar receiver (1.3) and can be one of screwed and locked onto the support element (1).
22. In the bus bar holder in accordance with claim 21, wherein on an underside facing the support element (1) the cover (4) has hollow spaces (4.2) which are matched to the respective head pieces (3.3) of the adapter elements (3) for receiving in an inserted state and are arranged corresponding to various insertion positions of the adapter elements (3) in the support element (1).
23. In the bus bar holder in accordance with claim 22, wherein one of there is a hollow space (4.2) with holding structures for each said bus bar receiver (1.3) in the cover (4), and there are several individual hollow spaces (4.2) corresponding to the various insertion positions of the adapter elements (3).
24. In the bus bar holder in accordance with claim 11, wherein protrusions (4.5) for pressing each said bus bar (2) against the cover (4) are provided on the underside of the cover (4).

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method of determining tropomyosin in chitosan, which comprises performing said determination by an immunoassay method with said chitosan being in a state dissolved in an aqueous solution of an organic acid.
2. A method according to claim 1, wherein said immunoassay method is an ELISA method.
3. A method according to claim 2, wherein said ELISA is a sandwich technique making use of at least one anti-crustacean tropomyosin antibody as a primary antibody and at least one labeled antibody.
4. A method according to claim 3, wherein said labeled antibody is an enzyme-labeled anti-crustacean tropomyosin polyclonal antibody or an enzyme-labeled anti-immunoglobulin antibody.
5. A method according to claim 4, wherein said crustacean is a shrimp, and said enzyme is at least one enzyme selected from the group consisting of peroxidase, alkaline phosphatase and \u03b2-galactosidase.
6. A method according to claim 3, wherein said anti-crustacean tropomyosin antibody is coated on an inner wall of a container for determination.
7. A method according to claim 4, wherein said enzyme is peroxidase, and as a substrate for peroxidase, at least one substrate selected from o-phenylenediamine, diammonium 2,2\u2032-azino-bis(3-ethylbenzothiazolinesulfonate) or tetramethylbenzidine is used.
8. A method according to claim 7, wherein said determination of tropomyosin is performed by measuring a blue color in a determination system.
9. A method according to claim 8, wherein said determination of tropomyosin is performed by adding sulfuric acid or phosphoric acid to a determination system, which produces a blue color, to change said determination system into a yellow color and then measuring said yellow color.
10. A method according to claim 1, wherein said organic acid is at least one organic acid selected from the group consisting of acetic acid, lactic acid and pyrrolidonecarboxylic acid.
11. A method according to claim 1, wherein a concentration of said organic acid in said aqueous solution of said organic acid is 0.1 wt. % or higher but lower than 2 wt. %.
12. A method according to claim 1, wherein said chitosan is chitosan having a content of insolubles not higher than 1.0 wt. % as measured by a measuring method which comprises:
(1) drying chitosan at 105\xb0 C. for 3 hours,
(2) calculating a purity of said chitosan from its weight ratio before and after said drying,
(3) dissolving said pre-drying chitosan in a 1 wt. % aqueous solution of acetic acid to give a pure chitosan (A gram) concentration of 0.5 wt. %,
(4) filtering the resultant aqueous solution through a G3 glass filter (B grams) which has been dried to a constant weight,
(5) washing with distilled water a filtration residue on said filter,
(6) drying said filter and said filtration residue contained thereon at 105\xb0 C. for 3 hours, and conducting weighing (C grams), and
(7) calculating said content (wt. %) of insolubles in said chitosan in accordance with an equation (C\u2212B)A\xd7100.
13. Chitosan having a tropomyosin content of 100 ppm or lower as determined by a method of determining tropomyosin in chitosan, which comprises performing said determination by an immunoassay method with said chitosan being in a state dissolved in an aqueous solution of an organic acid.

1461126021-cf0f253b-d77d-493a-96e7-f0c635302578

1. An article comprising:
a body portion or an end portion of a food or beverage can comprising a metal substrate; and
a coating composition disposed thereon, wherein the coating composition includes an emulsion polymerized latex polymer, and wherein the emulsion polymerized latex polymer comprises a reaction product of an ethylenically unsaturated monomer component polymerized in the presence of an aqueous dispersion of a polyurethane resin.
2. The article of claim 1, wherein the article comprises a 2-piece drawn food can body, a 3-piece food can body, a food can end, a drawn and ironed can body, a beverage can body, or a beverage can end.
3. The article of claim 1, wherein the polyurethane resin includes anionic salt groups, cationic salt groups, or a combination thereof.
4. The article of claim 1, wherein the polyurethane resin comprises a salt of an acid- or anhydride-functional polyurethane resin.
5. The article of claim 1, wherein the polyurethane resin comprises a salt of an acid- or anhydride-functional polyurethane resin and an amine.
6. The article of claim 1, wherein the polyurethane resin has a number average molecular weight of 1,500 to 50,000.
7. The article of claim 1, wherein the polyurethane resin is present in an amount comprising at least 5 wt-%, based on the total weight of the ethylenically unsaturated monomer component and the polyurethane resin.
8. The article of claim 1, wherein the ethylenically unsaturated component comprises at least about 25 wt-% of the emulsion polymerized latex polymer, based on the total weight of the ethylenically unsaturated monomer component and the polyurethane resin.
9. The article of claim 1, wherein the ethylenically unsaturated monomer component is polymerized in the presence of the aqueous dispersion with a water-soluble free radical initiator at a temperature of 0\xb0 C. to 100\xb0 C.
10. The article of claim 9, wherein the free radical initiator comprises a redox initiator system.
11. The article of claim 1, wherein the ethylenically unsaturated component has a number average molecular weight of at least 100,000.
12. The article of claim 1, wherein the emulsion polymerized latex polymer is substantially free of bound bisphenol A and aromatic glycidyl ether compounds.
13. The article of claim 1, wherein the coating composition is present on a food-contact surface of the metal substrate.
14. The article of claim 1, wherein the coating composition, when present on a beverage can end at a dry film thickness of 7 milligrams per square inch, passes less than 10 milliamps of current after being exposed for 4 seconds to a room-temperature electrolyte solution containing 1% by weight of NaCl dissolved in water.
15. A method, comprising:
providing a coating composition including an emulsion polymerized latex polymer, wherein the emulsion polymerized latex polymer comprises a reaction product of an ethylenically unsaturated monomer component polymerized in the presence of an aqueous dispersion of a polyurethane resin; and
forming a food or beverage can or portion thereof from a metal substrate having the coating composition disposed on at least one surface.
16. A method, comprising:
providing a composition comprising an emulsion polymerized latex polymer, wherein the emulsion polymerized latex polymer comprises the reaction product of:
an aqueous dispersion of a salt of an acid- or anhydride-functional polyurethane resin and an amine; and
an ethylenically unsaturated monomer component;
wherein the ethylenically unsaturated monomer component is polymerized in the presence of the aqueous dispersion to form the emulsion polymerized latex polymer, which is substantially free of bound bisphenol A and aromatic glycidyl ether compounds; and

applying the composition comprising the emulsion polymerized latex polymer to a metal substrate prior to or after forming the metal substrate into a food or beverage can or portion thereof.
17. The method of claim 16, wherein the amine comprises a tertiary amine.
18. The method of claim 16, wherein the ethylenically unsaturated monomer component is polymerized in the presence of the aqueous dispersion with a water-soluble free radical initiator.
19. The method of claim 18, wherein the ethylenically unsaturated monomer component is batch polymerized in the presence of the aqueous dispersion.
20. The method of claim 16, wherein the polyurethane resin is present in an amount from at least 5 wt-% to no greater than 95 wt-%, based on the total weight of the ethylenically unsaturated monomer component and the polyurethane resin.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A staple cartridge comprising:
a cartridge main body;
a staple strike out portion;
a connected staple containing portion provided on one end side;
a staple strike out portion provided on the other end side;
a guide portion for guiding connected staples at inside of the connected staple containing portion to the staple strike out portion;
a first actuator capable of entering and exiting to and from the guide portion; and
a second actuator capable of entering and exiting to and from an inside of the connected staple containing portion;
wherein the first actuator and the second actuator are interlocked,
wherein the connected staple containing portion contains a refill that contains connected staples inside thereof and is interchangeably attached to the cartridge main body, and
the second actuator is capable of entering and exiting to and from the inside of the refill.
2. The staple cartridge according to claim 1, wherein the cartridge main body is attached and detached to and from the stapler main body,
the first actuator is operated in accordance with a presenceabsence of the connected staples at an inside of the guide portion, and
the second actuator is operated in accordance with a presenceabsence of the connected staples at the inside of the connected staple containing portion.
3. The staple cartridge according to claim 2, wherein the second actuator is pivoted around a second middle portion,
the first actuator is pivoted around a first middle portion,
the first actuator and the second actuator are aligned in series, and
respective end portions of the first actuator and the second actuator contiguous to each other are connected to be engaged such that by pivoting one thereof, the other thereof is interlocked.
4. A staple cartridge comprising:
a cartridge main body;
a staple strike out portion;
a connected staple containing portion provided on one end side;
a staple strike out portion provided on the other end side;
a guide portion for guiding connected staples at inside of the connected staple containing portion to the staple strike out portion;
a first actuator capable of entering and exiting to and from the guide portion; and
a second actuator capable of entering and exiting to and from an inside of the connected staple containing portion;
wherein the first actuator and the second actuator are interlocked,
wherein the second actuator is pivoted around a second middle portion,
the first actuator is pivoted around a first middle portion,
the first actuator and the second actuator are aligned in series, and
respective end portions of the first actuator and the second actuator contiguous to each other are connected to be engaged such that by pivoting one thereof, the other thereof is interlocked.
5. A staple cartridge comprising:
a cartridge main body;
a connected staple containing portion provided on one end side;
a staple strike out portion provided on the other end side;
a guide portion for guiding connected staples at inside of the connected staple containing portion to the staple strike out portion;
a first actuator capable of entering and exiting to and from the guide portion; and
a second actuator capable of entering and exiting to and from an inside of the connected staple containing portion;
wherein the first actuator and the second actuator are interlocked,
the second actuator is pivoted around a second middle portion,
the first actuator is pivoted around a first middle portion,
the first actuator and the second actuator are aligned in series,
respective end portions of the first actuator and the second actuator contiguous to each other are connected to be engaged such that by pivoting one thereof, the other thereof is interlocked; and
connected staples contained in the staple cartridge.
6. A staple cartridge comprising:
a cartridge main body;
a connected staple containing portion provided on one end side;
a staple strike out portion provided on the other end side;
a guide portion for guiding connected staples at inside of the connected staple containing portion to the staple strike out portion;
a first actuator capable of entering and exiting to and from the guide portion; and
a second actuator capable of entering and exiting to and from an inside of the connected staple containing portion;
wherein the first actuator and the second actuator are interlocked,
the second actuator is pivoted around a second middle portion,
the first actuator is pivoted around a first middle portion,
the first actuator and the second actuator are aligned in series,
respective end portions of the first actuator and the second actuator contiguous to each other are connected to be engaged such that by pivoting one thereof, the other thereof is interlocked; and
a refill contained in the staple cartridge.
7. A staple cartridge comprising:
a cartridge main body;
a connected staple containing portion provided on one end side;
a staple strike out portion provided on the other end side;
a guide portion for guiding connected staples at inside of the connected staple containing portion to the staple strike out portion;
a first actuator capable of entering and exiting to and from the guide portion; and
a second actuator capable of entering and exiting to and from an inside of the connected staple containing portion;
wherein the first actuator and the second actuator are interlocked,
the second actuator is pivoted around a second middle portion,
the first actuator is pivoted around a first middle portion,
the first actuator and the second actuator are aligned in series,
respective end portions of the first actuator and the second actuator contiguous to each other are connected to be engaged such that by pivoting one thereof, the other thereof is interlocked; and
connected staples contained in a refill.