Author: claimsbot

1. A method for producing silicone hydrogel contact lenses, comprising the steps of:
(1) obtaining a fluid composition, wherein the composition comprises at least one prepolymer having multiple cinnamic acid moieties of formula (IV) or dialkylmaleimide groups of formula (V) each capable of undergoing photo-induced step-growth polymerization that is 2+2 cycloaddition to form a silicone hydrogel material,
\u2003in which
R20 is C1-C10 alkene divalent radical, R21 and R25 independent each other are hydrogen, C1-C10 alkyl or \u2014NO2 provided that either R21 or R25 is \u2014NO2, R22-R24 independent of one another are hydrogen or C1-C10 alkyl; R26 and R27, independent of each other, are C1-C10 alkyl and R28 is a C1-C10 alkene divalent radical;

\u2003wherein the prepolymer is obtained from a siloxane-containing copolymer with pendant or terminal functional groups by covalently attaching the cinnamic acid moieties of formula (IV) or dialkylmaleimide groups of formula (V) to the pendant or terminal functional groups of the copolymer;
(2) introducing the fluid composition into a cavity formed by a mold, wherein the mold has a first mold half with a first molding surface defining the anterior surface of a contact lens and a second mold half with a second molding surface defining the posterior surface of the contact lens, wherein said first and second mold halves are configured to receive each other such that a cavity is formed between said first and second molding surfaces; and
(3) actinically irradiating the composition in the mold to crosslink said at least one prepolymer to form a silicone hydrogel contact lens by 2+2 cycloaddition.
2. The method of claim 1, wherein the prepolymer comprises multiple cinnamic acid moieties of formula (IV)
in which
R20 is C1-C10 alkene divalent radical, R21 and R25 independent each other are hydrogen, C1-C10 alkyl or \u2014NO2 provided that either R21 or R25 is \u2014NO2, R22-R24 independent of one another are hydrogen or C1-C10 alkyl.
3. The method of claim 1, wherein the prepolymer comprises multiple dialkylmaleimide groups of formula (V),
in which R26 and R27, independent of each other, are hydrogen or C1-C10 alkyl and R28 is a C1-C10 alkene divalent radical.
4. The method of claim 1, wherein the mold is a reusable mold.
5. The method of claim 4, wherein the step (3) is performed by means of a spatial limitation of actinic radiation to crosslink the prepolymer.
6. A prepolymer suitable for making soft silicone hydrogel contact lenses, comprising: multiple cinnamic acid moieties of formula (IV) or multiple dialkylmaleimide groups of formula (V),
in which R20 is C1-C10 alkene divalent radical, R21 and R25 independent each other are hydrogen, C1-C10 alkyl or \u2014NO2 provided that either R21 or R25 is \u2014NO2, R22-R24 independent of one another are hydrogen or C1-C10 alkyl R26 and R27, independent of each other, are C1-C10 alkyl and R28 is a C1-C10 alkene divalent radical;
wherein the prepolymer is obtained from a copolymer with pendant or terminal functional groups by covalently attaching cinnamic acid moieties of formula (IV) or dialkylmaleimide groups of formula (V) to the copolymer through the pendant or terminal functional groups, wherein the copolymer with pendant or terminal functional groups comprises at least one siloxane units, wherein the functional group is selected from the group consisting of hydroxyl groups (\u2014OH), primary amino groups (\u2014NH2), secondary amino groups (\u2014NHR), carboxylic groups (\u2014COOH), epoxy groups, aldehyde groups (\u2014CHO), amide groups (\u2014CONH2), acid halide groups (\u2014COX, X\u2550Cl, Br, or I), isothiocyanate groups, isocyanate groups, halide groups (\u2014X, X\u2550CI, Br, or I), acid anhydride groups, and combinations thereof;
wherein the prepolymer is capable of being crosslinked under actinic irradiation to form a silicone hydrogel material in the presence of or in the absence of a step-growth-propagating agent and in the absence of any vinylic monomer andor any compound having from two to eight acryloyl or methacryloyl groups and having a molecular weight of less than 700 Daltons.
7. The prepolymer of claim 6, wherein the copolymer with pendant or terminal functional groups is obtained by copolymerization of a composition comprising
(1) at least one hydrophilic vinylic monomer,
(2) at least one silicone-containing monomer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having two or more ethylenically unsaturated double bonds, a polysiloxane having two or more ethylenically unsaturated double bonds, a polysiloxaneperfluoroalkyl polyether block copolymer having two or more ethylenically unsaturated double bonds, or a combination of two or more thereof,
(3) optionally at least one hydrophobic vinylic monomer; and
(4) optionally one or more hydrophilic prepolymers having multiple acryloyl or methacryloyl groups, provided that at least one of components (1)-(4) further comprises at least one functional group through which a cinnamic acid moiety or a dialkylmaleimide group can be covalently linked to the obtained copolymer.
8. The prepolymer of claim 6, wherein the copolymer with pendant or terminal functional groups is obtained by copolymerization of a composition comprising
(1) at least one hydrophilic vinylic monomer,
(2) at least one silicone-containing monomer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having two or more ethylenically unsaturated double bonds, a polysiloxane having two or more ethylenically unsaturated double bonds, a polysiloxaneperfluoroalkyl polyether block copolymer two or more ethylenically unsaturated double bonds, or a combination of two or more thereof,
(3) optionally at least one hydrophobic vinylic monomer;
(4) optionally one or more hydrophilic prepolymers having multiple acryloyl or methacryloyl groups, and
(5) at least one chain transfer agent having a functional group through which a cinnamic acid moiety or a dialkylmaleimide group can be covalently linked to the obtained copolymer.
9. The prepolymer of claim 6, wherein the copolymer is a copolymerization product of a polymerizable composition comprising
(a) at least one fluorine-containing vinylic monomer,
(b) at least one hydrophilic vinylic monomer,
(c) at least one functionalizing vinylic monomer containing at least one functional group through which a cinnamic acid moiety or a dialkylmaleimide group can be covalently linked to the obtained copolymer, and
(d) at least one silicone-containing monomer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having two or more ethylenically unsaturated double bonds, a polysiloxane having two or more ethylenically unsaturated double bonds, a polysiloxaneperfluoroalkyl polyether block copolymer two or more ethylenically unsaturated double bonds, or a combination of two or more thereof.
10. The prepolymer of claim 6, wherein the copolymer is a copolymerization product of a polymerizable composition comprising
(a) at least one fluorine-containing vinylic monomer,
(b) at least one hydrophilic vinylic monomer,
(c) at least one silicone-containing monomer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having one ethylenically unsaturated double bond, at least one siloxane-containing macromer having two or more ethylenically unsaturated double bonds, a polysiloxane having two or more ethylenically unsaturated double bonds, a polysiloxaneperfluoroalkyl polyether block copolymer two or more ethylenically unsaturated double bonds, or a combination of two or more thereof, and
(d) at least one chain transfer agent having a functional group through which a cinnamic acid moiety or a dialkylmaleimide group can be covalently linked to the obtained copolymer.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

I claim:

1. A system for administrating over changes to a record shared by a plurality of users authorized to do so, comprising:
a resource to officiate over an official version of the record and to communicate with the plurality of users over a medium for non-persistent communications;
in response to a request of a given user, a communication by the resource sending a copy of the official version;
in consequence of a return by the given user of an original edition of the sent copy as well as a request to effect given changes, the resource comparing the returned original edition of the sent copy to the official version so if in case of a match the resource effects the given changes to the official version, else not.
2. The system of claim 1 wherein the request to effect given changes comprises a changed edition of the sent copy and the resource effecting the given changes to the official version comprises replacing the official version with the changed edition.
3. The system of claim 1 wherein official version of the record is stored on storage devices administrated over or accessible to the resource.
4. The system- of claim 1 wherein the system activity of the resource comparing for a match comprises any of bit-by-bit comparison in full or in part, or a count-and-sum word comparison, or another algorithm for substantial identity.
5. The system of claim 1 wherein the system activity of else not further comprises informing the given user of the failure to effect the given changes.
6. The system of claim 1 wherein the resource comprises a server domain on the Internet global computer information network.
7. The system of claim 1 wherein the medium for non-persistent communications includes the Internet global computer information network.
8. A method for administrating over changes to a record shared by a plurality of users authorized to do so, comprising the steps of:
providing a resource to officiate over an official version of the record and to communicate with the plurality of users over a medium for non persistent communications;
in response to a request of a given user, the resource sending the given user a copy of the official version and storing another copy in storage as associated with the given user;
in consequence of a return by the given user of a request to effect given changes, the resource comparing the stored copy associated with the given user to the official version so if in case of a match the resource effects the given changes to the official version, else not.
9. The method of claim 8 wherein storing said other copy in storage comprises either storing said other copy on a device of the given user’s or else on another device including any devices administrated over or accessible to the resource.
10. The method of claim 8 wherein said resource converts said other copy into a changed format before storing in storage, including any of count-and-sum word values or abbreviated formats.
11. The method of claim 10 wherein the step of the resource comparing the stored copy associated with the given user to the official version comprises either the resource restoring the stored copy from the changed format or converting a copy of the official version into the changed format.
12. The method of claim 11 wherein the step of the resource comparing for a match comprises any of bit-by-bit comparison in full or in part, a count-and-sum word comparison, or another algorithm for substantial identity.
13. The method of claim 8 wherein the request to effect given changes comprises a changed edition of the one copy and the resource effecting the given changes to the official version comprises replacing the official version with the changed edition.
14. The method of claim 8 wherein the step of else not further comprises informing the given user of the failure to effect the given changes.
15. A method for administrating over changes to a record shared by a plurality of users authorized to do so, comprising the steps of:
providing a resource to officiate over an official version of the record and to communicate with the plurality of users over a medium for non-persistent communications;
in response to a request of a given user, the resource sending the given user a use copy of the official version as well as a transparent or opaque copy;
the given user accepting the use and transparent or opaque copies of the official version and thereafter applying to change the official version by returning the transparent or opaque copy of the official version as well as a request to effect changes;
the resource accepting the returned transparent or opaque copy of the official version as well as the request to effect changes and comparing the returned transparent or opaque copy to the official version so if in case of a match the resource effects the given changes to the official version, else not.
16. The method of claim 15 wherein accepting said transparent or opaque copy of the official version comprises the given user accepting a cookie.
17. The method of claim 15 wherein said resource converts said transparent or opaque copy of the official version into a changed format before sending, which changed format can include any of count-and-sum word values or encrypted or abbreviated formats.
18. The method of claim 17 wherein the step of the resource comparing the returned transparent or opaque copy of the official version comprises either the resource restoring the returned transparent or opaque copy of the official version from the changed format or converting a copy of the official version into the changed format.
19. The method of claim 15 wherein the request to effect given changes comprises a changed edition of the use copy and the resource effecting the given changes to the official version comprises replacing the official version with the changed edition.
20. The method of claim 15 wherein the step of else not further comprises informing the given user of the failure to effect the given changes.

What is claimed is:

1. Method for measuring the binding of analyte molecules to probe molecules, the method comprising the following steps:
(a) providing a circuit surface with electronic circuits,
(b) providing areas with covalently bound probe molecules, located in spatial proximity to the electronic circuits,
(c) binding analyte molecules to the probe molecules and, together with the analyte molecules, electrically conductive nanoparticles, and
(d) making the circuits of the circuit surface electrically reading the presence of the nanoparticles and thereby detecting the binding of the analyte molecules.
2. Method according to claim 1, wherein the reading by a circuit in step (d) consists of reading the changed stray capacitance near the circuit.
3. Method according to claim 1, wherein the reading by a circuit in step (d) consists of reading a voltage on the nanoparticles after contacting the nanoparticles to a contact spot of the circuit.
4. Method according to claim 1, wherein the probe molecules are bound to areas of the circuit surface in spatial proximity to the electronic circuits.
5. Method according to claim 1, wherein the probe molecules are bound to areas in spatial proximity to the electronic circuits, the areas located on the surface of a countersurface, positioned opposite the circuit surface.
6. Method according to claim 1, wherein the probe molecules are covalently bound to the surface and, in step (c), the analyte molecules are affinity bound to the probe molecules.
7. Method according to claim 1, wherein the nanoparticles are already bound to the analyte molecules.
8. Method according to claim 1, wherein in a first part of step (c), analyte molecules are bound to surface-bound probe molecules and in a second part of step (c), nanoparticles with adhesion molecules fixed to them are attached to the bound analyte molecules.
9. Method according to claim 3, wherein the nanoparticles are pressed against a contact spot of the circuit by a movement of the countersurface, and wherein a voltage applied to the countersurface can be measured via the nanoparticles in the circuits of the circuit surface.
10. Method according to claim 3, wherein a galvanic element is created by the metal surface of the bound nanoparticles, by a metal counterelectrode spot of limited size on the circuit and by a suitable electrolyte, which, by contact of the nanoparticles with contact spots separated from the counterelectrode spots, generates an electric current in the circuit surface from the contact spots towards the counterelectrode spots, enabling the binding of analyte molecules to be measured.
11. Method according to claim 10, wherein the contact between nanoparticles and contact spot is made by electrically conductive molecules.
12. Method according to claim 11, wherein the electrically conductive molecules are compounds of the polyene class.
13. Method according to claim 9, wherein the contact is made by the nanoparticles touching the contact spot.
14. Method according to claim 13, wherein analyte molecules and nanoparticles are bound to probe molecules located on an insulating surface opposite the circuit surface, and the contact of the nanoparticles with the contact spots is made by pressing the insulating surface with the bound nanoparticles onto the contact spots of the circuit surface.
15. Method according to claim 13, wherein analyte molecules and magnetizable nanoparticles are bound to probe molecules located on a surface opposite the circuit surface; the linkages between nanoparticles and analyte molecules or the linkages between the analyte molecules and the probe molecules are broken; and the contact of the now no longer immobilized nanoparticles with the contact spots of the circuit surface is made by an external magnetic field acting on the nanoparticles.
16. Method according to claim 13, wherein analyte molecules and magnetizable nanoparticles are bound to probe molecules located on the contact spots of the circuit surface, and the contact of the nanoparticles with the contact spots is made by the effect of an external magnetic field or by mechanical pressure of a countersurface on the nanoparticles.
17. Method according to claim 13, wherein the circuit surface or the surface of the nanoparticles is loaded with electrically conductive protrusions.
18. Method according to claim 1, wherein DNA oligomers are used as probe molecules, the analyte molecules are amplified in a previous step by polymerase chain reactions (PCR) using a biotinylated primer, and the nanoparticles are bound to the biotin groups of the analyte molecules by being coated with streptavidin.
19. Method according to claim 18, wherein instead of the biotin-streptavidin binding pair another binding pair is used.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A system for sealing two linearly connected pipes of a pipeline system, the system comprising:
a single layer fluid-tight tube that is inserted into an area of a gap between the two linearly connected pipes of the pipeline system to be sealed;
a feed element having a valve for introducing a fluid into the tube; and
a pump for conveying the fluid, the pump being connectable to the feed element,
wherein the fluid is introduced into the tube for increasing the volume of the tube, and thereby the tube filled with the fluid directly contacts and presses walls of the two linearly connected pipes and seals the gap between the two linearly connected pipes, and
wherein the tube contacts the pipes at at least three surfaces of the pipes.
2. The system for sealing a pipeline system according to claim 1, wherein the tube contains curable or weldable material.
3. The system for sealing a pipeline system according to claim 2, wherein the tube is connectable to form a ring by curing or welding.
4. The system for sealing a pipeline system according to claim 1, wherein the tube is formed of a material with a fabric insert of textile or metal.
5. The system for sealing a pipeline system according to claim 4, wherein the fabric insert is formed multilayer.
6. The system for sealing a pipeline system according to claim 1, wherein the tube is formed and provided for insertion into the gap.
7. The system for sealing a pipeline system according to claim 6, wherein the tube has a resilient collar that is configured to be insertable in the gap between the tube and an interior of the pipeline system.
8. The system for sealing a pipeline system according to claim 7, wherein the resilient collar is formed substantially of steel, particularly ship or stainless steel such as V4A steel.
9. The system for sealing a pipeline system according to claim 7, wherein the resilient collar is pretensioned.
10. The system for sealing a pipeline system according to claim 1, wherein the tube is formed and provided for arrangement on an inside wall in the area of the gap so that the tube is formed band-shaped with a width greater than 10 cm and in a fluid-filled state covers the gap on the inside wall in a flat manner.
11. The system for sealing a pipeline system according to claim 1, wherein the tube has a plurality of chambers extending in a longitudinal direction of the tube.
12. The system for sealing a pipeline system according to claim 1, wherein the pipes to be sealed from one another form a coupling, whose inner pipe has a pipe end with a phase disposed at the outer diameter.
13. The system for sealing a pipeline system according to claim 1, wherein the pipes to be sealed from one another form a bell socket, with a plastic seal between the bell socket and an associated other pipe end.
14. The system for sealing a pipeline system according to claim 1, further comprising a protective ring arranged on an inside wall in an area of the pipe gap provided with the tube.
15. The system for sealing a pipeline system according to claim 14, wherein the protective ring is formed from a plurality of segments, which are connectable to one another to form the protective ring with an outside diameter corresponding to an inside diameter of the annular gap to be sealed.
16. The system for sealing a pipeline system according to claim 15, wherein the segments are connectable by movable bolts to form the protective ring.
17. The system for sealing a pipeline system according to claim 16, wherein the bolts are guided axially in a guide, and wherein, in a connected state, the guide is formed by a common guide on two different segments.
18. The system for sealing a pipeline system according to claim 17, wherein the bolts in the connected state are secured from inadvertent opening.
19. The system for sealing a pipeline system according to claim 14, wherein the protective ring andor individual segments of the protective ring are pretensioned.
20. The system for sealing a pipeline system according to claim 14, wherein the protective ring is formed wavelike in cross section.
21. The system for sealing a pipeline system according to claim 1, wherein the fluid is an inert gas, nitrogen, carbon dioxide, a non-reactive liquid, or liquid water.
22. The system for sealing a pipeline system according to claim 1, further comprising
a protective ring arranged on an inside wall in an area of the pipe gap provided with the tube; and
a ring fillable with a fluid arranged on the protective ring.
23. A system for sealing a pipeline system, the system comprising:
a fluid-tight tube configured for insertion into an area of a gap between two pipes of the pipeline system to be sealed;
a feed element having a valve for introducing a fluid into the tube;
a pump for conveying the fluid, the pump being connectable to the feed element; and
a protective ring arranged on an inside wall in an area of the pipe gap provided with the tube,
wherein the protective ring is formed wavelike in cross section, and
wherein a ring fillable with a fluid is arranged in one or more troughs of the protective ring.