1-21. (canceled)
22. An isolated polypeptide having protease activity, selected from the group consisting of:
(a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2 or 4;
(b) a polypeptide encoded by a polynucleotide that hybridizes under medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1 or 3, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO: 1 or 3, or (iii) the full-length complementary strand of (i) or (ii);
(c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 1 or 3;
(d) a variant comprising a substitution, deletion, andor insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO: 2 or 4;
(e) a fragment of a polypeptide of (a), (b), (c), or (d) that has protease activity; and
(f) a polypeptide having protease activity and further comprising the motif:
(SEQ\u2003ID\u2003NO:\u200313)
His\u2003Gly\u2003Thr\u2003(Xaa)6\u2003Ala\u2003Ser\u2003Tyr\u2003Gly\u2003Ser\u2003Val\u2003Ser\u2003Gly
wherein Xaa is any natural amino acid and (Xaa)6 mean 6 consecutive amino acids, where each amino acid may be any natural amino acid;
or the corresponding motif comprising one or two substitutions among the last 8 amino acids in the motif, such as a motif selected among SEQ ID NO: 14 to SEQ ID NO: 49.
23. The polypeptide of claim 22, having at least 80% sequence identity to the mature polypeptide of SEQ ID NO: 2 or 4.
24. The polypeptide of claim 22, having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2 or 4.
25. The polypeptide of claim 22, comprising or consisting of SEQ ID NO: 2 or 4.
26. The polypeptide of claim 22, comprising or consisting of the mature polypeptide of SEQ ID NO: 2 or 4.
27. The polypeptide of claim 26, wherein the mature polypeptide is amino acids 144 to 418 of SEQ ID NO: 2 or amino acids 1 to 275 of SEQ ID NO: 4.
28. The polypeptide of claim 22, comprising a S8 serin protease domain.
29. A polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO: 2, 4 or 6, wherein said polypeptide comprises the motif His Gly Thr (Xaa)6 Ala Ser Tyr Gly Ser Val Ser Gly (SEQ ID NO: 13).
30. The polypeptide of claim 22, wherein the protease has high detergency performance at low temperatures and the stability is not affected by the presence of a strong chelator.
31. An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
(a) a catalytic domain having at least 60% sequence identity to amino acids 143 to 418 of SEQ ID NO: 2
(b) a catalytic domain encoded by a polynucleotide that hybridizes under medium-high stringency conditions with (i) nucleotides 711 to 1535 of SEQ ID NO: 1, (ii) cDNA sequence comprising the catalytic domain of SEQ ID NO: 1, or (iii) the full-length complementary strand of (i) or (ii);
(c) a catalytic domain encoded by a polynucleotide having at least 60% sequence identity to the catalytic domain of SEQ ID NO: 1;
(d) a variant of a catalytic domain comprising a substitution, deletion, andor insertion of one or more (several) amino acids of the catalytic domain of SEQ ID NO: 2; and
(e) a fragment of a catalytic domain of (a), (b), (c) or (d) that has protease activity.
32. A composition comprising the polypeptide of claim 22.
33. The composition of claim 32, which is a detergent composition for laundry or automatic dish washing.
34. The composition of claim 33, further comprising one of more additional enzymes selected from the group consisting of a protease, an amylase, a lipase, a cutinase, a cellulase, an endoglucanase, a xyloglucanase, a pectinase, a pectin lyase, a xanthanase, a peroxidase, a haloperoxygenase, a catalase, a mannanase, or any mixture thereof.
35. The composition of claim 32, comprising a surfactant.
36. An isolated polynucleotide encoding the polypeptide of claim 22.
37. A nucleic acid construct or expression vector comprising the polynucleotide of claim 36 operably linked to one or more (several) control sequences that direct the production of the polypeptide in an expression host.
38. A recombinant host cell comprising the polynucleotide of claim 36 operably linked to one or more control sequences that direct the production of the polypeptide.
39. A method of producing the polypeptide of claim 22, comprising:
(a) cultivating a cell, which in its wild-type form produces the polypeptide, under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
40. A method of producing a polypeptide, comprising:
(a) cultivating a host cell of claim 38 under conditions conducive for production of the polypeptide; and
(b) recovering the polypeptide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A correlated set of golf clubs, comprising:
at least a first group of golf clubs, wherein each of the clubs in the first group comprises a club head having a main body of a first material having a first density;
at least a second group of golf clubs, wherein each of the clubs in the second group comprises a club head having a main body of a second material having a second density higher than the first density;
wherein the first group of clubs comprise lower numbered clubs than the second group of clubs.
2. The correlated set of clubs of claim 1, wherein:
each of the clubs heads of the first group of clubs comprises a face, a sole, and a rear cavity surrounded by a peripheral belt of mass, and a separate weight insert of material having a higher density than the first density, the weight insert positioned on a lower portion of the peripheral belt of mass so as to shift the center of gravity of the each of the club heads of the first group toward the sole; and
each of the heads of the second group of clubs comprises a face, a sole, and a rear cavity surrounded by a unitary peripheral belt of mass.
3. The correlated set of golf clubs of claim 2, wherein the each of the club heads in the second group is made entirely of a single piece of the same type of one of metal and metal alloy.
4. The correlated set of golf clubs of claim 2, wherein each of the club heads of the second group includes at least one weight insert positioned on a lower portion of the peripheral belt of mass of the club heads of the second group.
5. The correlated set of golf clubs of claim 2, wherein the entire weight inserts of the club heads of the first group are positioned below an annular surface surrounding the rear cavities of the club heads of the first group.
6. The correlated set of golf clubs of claim 4, wherein the entire weight inserts of the club heads of the second group are positioned below an annular surface surrounding the rear cavities of the club heads of the second group.
7. The correlated set of golf clubs of claim 2, wherein each of the club heads of the second group further comprises a separate face insert.
8. The correlated set of golf clubs of claim 7, wherein the face insert of each of the club heads of the second group is manufactured of one of titanium and a titanium alloy.
9. The correlated set of golf clubs of claim 7, wherein the face insert of each of the club heads of the second group is manufactured of one of aluminum, an aluminum alloy, steel, and a steel alloy.
10. The correlated set of golf clubs of claim 4, wherein the at least one weight insert of each of the club heads of the second group is made of a tungsten alloy with a density greater than 10 grams per cubic centimeter.
11. The correlated set of golf clubs of claim 2, wherein the first material comprises one of titanium and a titanium alloy, and wherein the weight inserts of the first set of clubs are made of tungsten with a density greater than 10 grams per cubic centimeter.
12. The correlated set of golf clubs of claim 2, wherein the second material comprises one of steel, a steel alloy, copper, a copper alloy, nickel, and a nickel alloy.
13. The correlated set of golf clubs of claim 1, additionally comprising:
a third group of clubs, wherein each of the clubs in the third group comprises a club head having a face and a sole wherein the clubs of the third group have lower numbers than the clubs of the second group and wherein the clubs heads of the third group are made of higher density material than the club heads of the first group.
14. The correlated set of golf clubs of claim 13, wherein the club heads of the third group are made of steel, a steel alloy, copper, a copper alloy, nickel, and a nickel alloy.
15. The correlated set of golf clubs of claim 14, wherein the club heads of the third group have a rear cavity surrounded by a peripheral belt of mass.
16. The correlated set of golf clubs of claim 13, wherein:
the first group of clubs comprises clubs from the set of 1, 2, 3, 4, 5, and 6 numbered clubs;
the second group of clubs comprises clubs from the set of 7, 8, and 9 numbered clubs and the pitching wedge; and
the third group of clubs comprises clubs from the set of the approach wedge, the sand wedge, and the lob wedge.
17. The correlated set of golf clubs of claim 13, additionally comprising:
a fourth group of clubs having higher numbers than the clubs of the third group of clubs, wherein each of the clubs in the fourth group comprises a club head having a face and a sole and wherein the club heads of the fourth group are made of higher density material than the club heads of the first group.
18. The correlated set of golf clubs of claim 17, wherein the club heads of the fourth group are made of steel.
19. The correlated set of golf clubs of claim 17, wherein:
the first group of clubs comprises clubs from the set of 1, 2, 3, 4 and 5 numbered clubs;
the second group comprises clubs from the set of 6 and 7 numbered clubs;
the third group comprises clubs from the set of 8 and 9 numbered clubs and a pitching wedge; and
the fourth group comprises clubs from the set of the approach wedge, the sand wedge, and the lob wedge.
20. The correlated set of golf clubs of claim 1, additionally comprising a single piece of packaging enclosing the set of golf clubs.
21. The correlated set of golf clubs of claim 2, wherein the faces of the club heads of the first group are larger than the faces of the club heads of the second group.
22. A set of golf clubs, comprising:
a first group of golf clubs, each of said first group of golf clubs having a head and a loft designation;
a second group of golf clubs, each of said second group of golf clubs having a head and a loft designation, wherein the loft designation of each of said first group of golf clubs and each of said second group of golf clubs is unique and wherein each loft designation of said second group of golf clubs is higher than each loft designation of said first group of golf clubs;
wherein each of said first group of golf clubs and said second group of golf clubs has a substantially same strike surface indicia and substantially the same loft designation typestyle and wherein the head of each of said first group of golf clubs differs from the head of each of said second group of golf clubs in at least one from the group of density and the existence of separate weight inserts.
23. A correlated set of golf clubs, comprising:
a first iron having a first club head, said first club head having a first loft designation and a first center of gravity located a first vertical distance from the ground plane when the club head is in address position;
a second iron having a second club head, said second club head having a second loft designation designating a higher loft than said first loft designation and a second center of gravity located a second vertical distance from the ground plane when the club head is in address position, said second vertical distance being longer than said first vertical distance; and
a third iron having a third club head, said third club head having a third loft designation designating a higher loft than said second loft designation and a third center of gravity located a third vertical distance from the ground plane when the club head is in address position, said third vertical distance being longer than said second vertical distance.
24. The correlated set of golf clubs of claim 23, further comprising:
a fourth iron having a fourth club head, said fourth club head having a fourth loft designation designating a higher loft than said third loft designation and a fourth center of gravity located a fourth vertical distance from the ground plane when the club head is in address position, said fourth vertical distance being longer than said third vertical distance; and
a fifth iron having a fifth club head, said fifth club head having a fifth loft designation designating a higher loft than said fourth loft designation and a fifth center of gravity located a fifth vertical distance from the ground plane when the club head is in address position, said fifth vertical distance being longer than said fourth vertical distance.
25. The correlated set of golf clubs of claim 24, further comprising:
a sixth iron having a sixth club head, said sixth club head having a sixth loft designation designating a higher loft than said fifth loft designation and a sixth center of gravity located a sixth vertical distance from the ground plane when the club head is in address position, said sixth vertical distance being longer than said fourth vertical distance; and
a seventh iron having a seventh club head, said seventh club head having a seventh loft designation designating a higher loft than said sixth loft designation and a seventh center of gravity located a seventh vertical distance from the ground plane when the club head is in address position, said seventh vertical distance being longer than said sixth vertical distance.
26. A correlated set of golf clubs, comprising:
a first iron having a first club head, said first club head having a loft designation 3 and a first center of gravity located a first vertical distance from the ground plane when the club head is in address position;
a second iron having a second club head, said second club head having a loft designation of one of 2 and 4 and a second center of gravity located a second vertical distance from the ground plane when the club head is in address position;
a third iron having a third club head, said third club head having a loft designation of 5 and a third center of gravity located a third vertical distance from the ground plane when the club head is in address position; and
a fourth iron having a fourth club head, said fourth club head having a loft designation of one of 4 and 6 and a fourth center of gravity located a fourth vertical distance from the ground plane when the club head is in address position, wherein each club head has a unique loft designation and said first vertical distance is shorter than either of said third vertical distance and said fourth vertical distance, and said second vertical distance is shorter than either of said third vertical distance and said fourth vertical distance.
27. The correlated set of golf clubs of claim 26, comprising:
a fifth iron having a fifth club head, said fifth club head having a loft designation of one of 6 and 7 and a fifth center of gravity located a fifth vertical distance from the ground plane when the club head is in address position, wherein each club head has a unique loft designation and said first vertical distance is shorter than said fifth vertical distance, and said second vertical distance is shorter than said fifth vertical distance.
28. A correlated set of golf clubs, comprising:
a first iron having a first club head, said first club head having a loft designation 3 and a first center of gravity located a first vertical distance from the ground plane when the club head is in address position;
a second iron having a second club head, said second club head having a loft designation 4 and a second center of gravity located a second vertical distance from the ground plane when the club head is in address position;
a third iron having a third club head, said third club head having a loft designation of 5 and a third center of gravity located a third vertical distance from the ground plane when the club head is in address position; and
a fourth iron having a fourth club head, said fourth club head having a loft designation 6 and a fourth center of gravity located a fourth vertical distance from the ground plane when the club head is in address position;
a fifth iron having a fifth club head, said fifth club head having a loft designation 7 and a fifth center of gravity located a fifth vertical distance from the ground plane when the club head is in address position, wherein none of said loft designations are identical and said first vertical distance is shorter than said fifth vertical distance, and said second vertical distance is shorter than said fifth vertical distance; and
a sixth iron having a sixth club head, said sixth club head having a loft designation 8 and a sixth center of gravity located a sixth vertical distance from the ground plane when the club head is in address position, each of said first vertical distance, said second vertical distance, said third vertical distance and said fourth vertical distance being at least as short as each of said fifth vertical distance and said sixth vertical distance.