Author: claimsbot

What is claimed is:

1. An isolated polynucleotide encoding a zsig63 polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 16 (Arg) to amino acid number 37 (Ser);
(b) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 38 (Leu) to amino acid number 126 (Ala);
(c) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 127 (Pro) to amino acid number 219 (Gln);
(d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gin); and
(e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 219 (Gln).
2. An isolated polynucleotide according to claim 1, wherein the zsig63 polypeptide comprises a sequence of amino acid residues selected from the group consisting of:
(a) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 16 (Arg) to amino acid number 37 (Ser);
(b) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 38 (Leu) to amino acid number 126 (Ala);
(c) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 127 (Pro) to amino acid number 219 (Gln);
(d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln); and
(e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 219 (Gln).
3. An isolated polynucleotide according to claim 1, wherein the polynucleotide is selected from the group consisting of:
(a) a polynucleotide sequence as shown in SEQ ID NO:1 from nucleotide 173 to nucleotide 784;
(b) a polynucleotide sequence as shown in SEQ ID NO:1 from nucleotide 128 to nucleotide 784; and
(c) a polynucleotide sequence complementary to (a) or (b).
4. An isolated polynucleotide sequence according to claim 1, wherein the polynucleotide comprises nucleotide 1 to nucleotide 657 of SEQ ID NO:3.
5. An expression vector comprising the following operably linked elements:
a transcription promoter;
a DNA segment encoding a zsig63 polypeptide comprising an amino acid sequence that is at least 90% identical to the amino acid sequence shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln); and
a transcription terminator.
6. An expression vector according to claim 5, further comprising a secretory signal sequence operably linked to the DNA segment.
7. A cultured cell into which has been introduced an expression vector according to claim 5, wherein the cell expresses a polypeptide encoded by the DNA segment.
8. A DNA construct encoding a fusion protein, the DNA construct comprising:
a first DNA segment encoding a polypeptide selected from the group consisting of:
(a) the amino acid sequence of SEQ ID NO: 2 from residue number 1 (Met) to residue number 15 (Ala);
(b) the amino acid sequence of SEQ ID NO: 2 from residue number 16 (Arg) to residue number 37 (Ser).;
(c) the amino acid sequence of SEQ ID NO: 2 from residue number 38 (Leu) to residue number 126 (Ala);
(d) the amino acid sequence of SEQ ID NO: 2 from residue number 127 (Pro) to residue number 219 (Gln); and
(e) the amino acid sequence of SEQ ID NO:2 from residue number 16 (Arg) to residue number 219 (Gln); and
at least one other DNA segment encoding an additional polypeptide,
wherein the first and other DNA segments are connected in-frame; and
encode the fusion protein.
9. A fusion protein produced by a method comprising:
culturing a host cell into which has been introduced a vector comprising the following operably linked elements:
(a) a transcriptional promoter;
(b) a DNA construct encoding a fusion protein according to claim 8; and
(c) a transcriptional terminator; and
recovering the protein encoded by the DNA segment.
10. An isolated zsig63 polypeptide comprising a sequence of amino acid residues that is at least 90% identical to an amino acid sequence selected from the group consisting of:
(a) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 16 (Arg) to amino acid number 37 (Ser);
(b) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 38 (Leu) to amino acid number 126 (Ala);
(c) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 127 (Pro) to amino acid number 219 (Gln);
(d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln); and
(e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 219 (Gln).
11. An isolated zsig63 polypeptide according to claim 10, wherein the polypeptide comprises a sequence of amino acid residues selected from the group consisting of:
(a) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 16 (Arg) to amino acid number 37 (Ser);
(b) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 38 (Leu) to amino acid number 126 (Ala);
(c) the amino acid sequence as shown in SEQ ID NO: 2 from amino acid number 127 (Pro) to amino acid number 219 (Gln);
(d) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 16 (Arg) to amino acid number 219 (Gln); and
(e) the amino acid sequence as shown in SEQ ID NO:2 from amino acid number 1 (Met) to amino acid number 219 (Gln).
12. A method of producing a zsig63 polypeptide comprising:
culturing a cell according to claim 7; and
isolating the zsig63 polypeptide produced by the cell.
13. A method of detecting, in a test sample, the presence of an antagonist of zsig63 protein activity, comprising:
transfecting a zsig63-responsive cell, with a reporter gene construct that is responsive to a zsig63-stimulated cellular pathway; and
producing a zsig63 polypeptide by the method of claim 12; and
adding the zsig63 polypeptide to the cell, in the presence and absence of a test sample; and
comparing levels of response to the zsig63 polypeptide, in the presence and absence of the test sample, by a biological or biochemical assay; and
determining from the comparison, the presence of the antagonist of zsig63 activity in the test sample.
14. A method of detecting, in a test sample, the presence of an agonist of zsig63 protein activity, comprising:
transfecting a zsig63-responsive cell, with a reporter gene construct that is responsive to a zsig63-stimulated cellular pathway; and
adding a test sample; and
comparing levels of response in the presence and absence of the test sample, by a biological or biochemical assay; and
determining from the comparison, the presence of the agonist of zsig63 activity in the test sample.
15. A method of producing an antibody to zsig63 polypeptide comprising the following steps in order:
inoculating an animal with a polypeptide selected from the group consisting of:
(a) a polypeptide consisting of 9 to 204 amino acids, wherein the polypeptide consists of a contiguous sequence of amino acids in SEQ ID NO:2 from amino acid number 16 (Ala) to amino acid number 219 (Gln); and
(b) a polypeptide according to claim 10;
(c) a polypeptide comprising amino acid number 16 (Arg) to 37 (Ser) of SEQ ID NO:2;
(d) a polypeptide comprising amino acid number 38 (Leu) to 126 (Ala) of SEQ ID NO:2;
(e) a polypeptide comprising amino acid number 127 (Pro) to 219 (Gln) of SEQ ID NO:2;
(f) a polypeptide comprising amino acid number 16 (Arg) to amino acid number 219 (Gln) of SEQ ID NO:2;
(g) a polypeptide comprising amino acid number 1 (Met) to amino acid number 219 (Gln) of SEQ ID NO:2;
(h) a polypeptide comprising amino acid number 14 (Phe) to 19 (Arg) of SEQ ID NO:2;
(i) a polypeptide comprising amino acid number 16 (Arg) to 21 (Phe) of SEQ ID NO:2;
(j) a polypeptide comprising amino acid number 24 (Gly) to 29 (Asp) of SEQ ID NO:2;
(k) a polypeptide comprising amino acid number 25 (Glu) to 30 (Asp) of SEQ ID NO:2;
(l) a polypeptide comprising amino acid number 187Glu) to 192 (Glu) of SEQ ID NO:2;
(m) a polypeptide comprising amino acid number 24 (Gly) to 33 (Pro) of SEQ ID NO:2;
(n) a polypeptide comprising amino acid number 17 (Lys) to 33 (Pro) of SEQ ID NO:2;
(o) a polypeptide comprising amino acid number 66 (Thr) to 73 (Pro) of SEQ ID NO:2;
(p) a polypeptide comprising amino acid number 103 (Pro) to 108 (Gly) of SEQ ID NO:2;
(q) a polypeptide comprising amino acid number 190 (Ala) to 197 (Glu) of SEQ ID NO:2;
(r) a polypeptide comprising amino acid number 202 (Lys) to 215 (Gly) of SEQ ID NO:2; and

(s) a polypeptide comprising amino acid number 190 (Ala) to 215 (Glu) of SEQ ID NO:2; and
wherein the polypeptide elicits an immune response in the animal to produce the antibody; and
isolating the antibody from the animal.
16. An antibody produced by the method of claim 15, which binds to a zsig63 polypeptide.
17. The antibody of claim 16, wherein the antibody is a monoclonal antibody.
18. An antibody that binds to a polypeptide of claim 10.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

I claim:

1. A vortex nozzle, comprising:
a nozzle body including a passageway therethrough and a plurality of ports that inlet a fluid flow into the passageway; and
an end cap attached to the nozzle body.
2. The vortex nozzle according to claim 1 wherein the passageway is a tapered passageway.
3. The vortex nozzle according to claim 2 wherein the tapered passageway includes an inlet side and an outlet side.
4. The vortex nozzle according to claim 3 wherein the inlet side of the tapered passageway includes a taper at an angle substantially equal to the angle of the taper of the tapered passageway.
5. The vortex nozzle according to claim 2 wherein each of the plurality of ports is tangential to the tapered passageway .
6. The vortex nozzle according to claim 2 wherein each of the plurality of ports enters the tapered passageway at an angle substantially equal to the angle of the taper of the tapered passageway.
7. The vortex nozzle according to claim 3 wherein the cross-sectional area of each of the plurality of ports is less than the cross-sectional area of the inlet side of the tapered passageway.
8. The vortex nozzle according to claim 1 wherein the plurality of ports is substantially equally spaced radially about the nozzle body.
9. The vortex nozzle according to claim 1 wherein the plurality of ports is substantially trapezoidal in shape.
10. The vortex nozzle according to claim 2 wherein the end cap includes an inner face having a taper at an angle substantially equal to the angle of the taper of the tapered passageway.
11. The vortex nozzle according to claim 1 wherein the end cap includes a boss that extends into the passageway and is adapted to adjust force vector components of the fluid flow entering the passageway.
12. The vortex nozzle according to claim 1 wherein the nozzle body is substantially cylindrical in shape and includes a shoulder having a raised portion.
13. A vortex nozzle, comprising:
a nozzle body including a passageway therethrough and a port that inlets a fluid flow into the passageway, whereby the port is tangential to the passageway; and
an end cap attached to the nozzle body.
14. The vortex nozzle according to claim 13 wherein the passageway is a tapered passageway.
15. The vortex nozzle according to claim 14 wherein the tapered passageway includes an inlet side and an outlet side.
16. The vortex nozzle according to claim 15 wherein the inlet side of the tapered passageway includes a taper at an angle substantially equal to the angle of the taper of the tapered passageway.
17. The vortex nozzle according to claim 14 wherein the port is tangential to the tapered passageway.
18. The vortex nozzle according to claim 14 wherein the port enters the tapered passageway at an angle substantially equal to the angle of the taper of the tapered passageway.
19. The vortex nozzle according to claim 15 wherein the cross-sectional area of the port is less than the cross-sectional area of the inlet side of the tapered passageway.
20. The vortex nozzle according to claim 13 wherein the port is substantially trapezoidal in shape.
21. The vortex nozzle according to claim 14 wherein the end cap includes an inner face having a taper at an angle substantially equal to the angle of the taper of the tapered passageway.
22. The vortex nozzle according to claim 13 wherein the end cap includes a boss that extends into the passageway and is adapted to adjust force vector components of the fluid flow entering the passageway.
23. The vortex nozzle according to claim 13 wherein the nozzle body is substantially cylindrical in shape and includes a shoulder having a raised portion.
24. A fluid treating apparatus, comprising:
a first vortex nozzle including a passageway therethrough and a plurality of ports that inlet a first fluid flow into the passageway, whereby the first vortex nozzle imparts a rotation to the first fluid flow thereby creating a first rotated fluid flow; and
a second vortex nozzle positioned in opposed relation the first vortex nozzle, the second vortex nozzle including a passageway therethrough and a plurality of ports that inlet a second fluid flow into the passageway, whereby the second vortex nozzle imparts a rotation to the second fluid flow thereby creating a second rotated fluid flow collided with the first rotated fluid flow.
25. A fluid treating apparatus, comprising:
a first vortex nozzle including a passageway therethrough and a port that inlets a first fluid flow tangentially to the passageway, whereby the first vortex nozzle imparts a rotation to the first fluid flow thereby creating a first rotated fluid flow; and
a second vortex nozzle positioned in opposed relation the first vortex nozzle, the second vortex nozzle including a passageway therethrough and a port that inlets a second fluid flow tangentially to the passageway, whereby the second vortex nozzle imparts a rotation to the second fluid flow thereby creating a second rotated fluid flow collided with the first rotated fluid flow.
26. The fluid treating apparatus according to claim 25 wherein the port of the first vortex nozzle inlets the first fluid flow normal to the passageway.
27. The fluid treating apparatus according to claim 25 wherein the port of the second vortex nozzle inlets the second fluid flow normal to the passageway.
28. A method of treating a fluid, comprising:
positioning a first vortex nozzle in opposed relation to a second vortex nozzle;
inletting a first fluid flow into a passageway of the first vortex nozzle via a plurality of ports of the first vortex nozzle, whereby the first vortex nozzle imparts a rotation to the first fluid flow thereby creating a first rotated fluid flow; and
inletting a second fluid flow into a passageway of the second vortex nozzle via a plurality of ports of the second vortex nozzle, whereby the second vortex nozzle imparts a rotation to the second fluid flow thereby creating a second rotated fluid flow collided with the first rotated fluid flow.
29. A method of treating a fluid, comprising:
positioning a first vortex nozzle in opposed relation to a second vortex nozzle;
inletting a first fluid flow tangentially into a passageway of the first vortex nozzle via a port of the first vortex nozzle, whereby the first vortex nozzle imparts a rotation to the first fluid flow thereby creating a first rotated fluid flow; and
inletting a second fluid flow tangentially into a passageway of the second vortex nozzle via a port of the second vortex nozzle, whereby the second vortex nozzle imparts a rotation to the second fluid flow thereby creating a second rotated fluid flow collided with the first rotated fluid flow.
30. The method according to claim 29, further comprising inletting the first fluid flow normal to the passageway of the first vortex nozzle via the port of the first vortex nozzle.
31. The method according to claim 29, further comprising inletting the second fluid flow normal to the passageway of the second vortex nozzle via the port of the second vortex nozzle.
32. A method of rotating a fluid, comprising:
inletting a fluid flow into a passageway of a vortex nozzle via a plurality of ports; and
rotating the fluid flow in the passageway.
33. The method according to claim 32, further comprising inletting the fluid flow tangentially to the passageway.
34. The method according to claim 32, wherein the passageway is tapered, and the fluid flow is inlet at an angle substantially equal to the taper.
35. A method of rotating a fluid, comprising:
inletting a fluid flow tangentially into a passageway of a vortex nozzle; and
rotating the fluid flow in the passageway.
36. The method according to claim 35, further comprising inletting the fluid flow normal to the passageway.
37. The method according to claim 35, wherein the passageway is tapered, and the fluid flow is inlet at an angle substantially equal to the taper.
38. A vortex nozzle, comprising:
a nozzle body including a passageway;
at least a segment of the passageway being tapered; and
a plurality of ports that inlet a fluid flow into the passageway.
39. The vortex nozzle according to claim 38, wherein at least one of the plurality of ports is tangential to the tapered passageway.
40. The vortex nozzle according to claim 38, wherein at least one of the plurality of ports enters the tapered passageway at an angle substantially equal to the angle of the taper of the tapered passageway.
41. The vortex nozzle according to claim 38, wherein the nozzle body is substantially cylindrical in shape and includes a shoulder having a raised portion.
42. A vortex nozzle, comprising:
a nozzle body including a passageway;
at least a segment of the passageway being tapered; and
a port that inlets a fluid flow into the passageway, the port being tangential to the passageway.
43. The vortex nozzle according to claim 42, wherein the port enters the tapered passageway at an angle substantially equal to the angle of the taper of the tapered passageway.
44. The vortex nozzle according to claim 42, wherein the nozzle body is substantially cylindrical in shape and includes a shoulder having a raised portion.
45. A fluid treatment system, comprising:
a pump;
two opposed vortex nozzles;
a manifold for receiving fluid from the pump and directing it to the nozzles; and
a frame, wherein the pump, nozzles, and manifold are mounted to the frame.
46. The system according to claim 45, wherein the manifold comprises two elbows, and each of the elbows comprises two elbow fittings.
47. A fluid treatment system, comprising:
a pump;
two opposed vortex nozzles; and
a manifold for receiving fluid from the pump and directing it to the nozzles, wherein
the manifold comprises two elbows, and each of the elbows comprises two elbow fittings.
48. The fluid treatment system according to claim 47, further comprising a frame on which the nozzles, pump, and manifold are mounted.

1. A reactive dyestuff with an N,N-dialkylamino bridge group of the following formula (I),
wherein, E1 and E2 each independently are couplers selected from the group consisting of benzene, naphthalene, pyrazolone, monoazo, disazo, polyazo and metal complex azo components;
R is hydrogen, C1-4 alkyl, C1-4 alkylcarbonyl, phenyl, nitroso or C1-4 alkyl substituted by halogen, hydroxyl, carboxyl or sulfo;
(R1)0-3 and (R2)0-3 each independently are 0 to 3 identical or different groups, and each of R1 and R2 independently is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, ureido and amido;
B and B\u2032 each independently are selected from the group consisting of a direct bond, \u2014SO2\u2014 and \u2014CONH\u2014(CH2)k\u2014SO2\u2014;
k is an integer between 1 to 6;
Z and Z\u2032 each independently are selected from the group consisting of \u2014SO2\u2014U, \u2014CONH\u2014(CH2)d\u2014SO2\u2014U, \u2014O\u2014(CH2)r\u2014CONH\u2014(CH2)d\u2014SO2\u2014U, \u03b2-thiosulfatoethylsulfonyl and \u2014N(R\u2032)\u2014U\u2032;
d and r are integers independent of one another between 2 to 4;
U is \u2014CH2CH2W or \u2014CH\u2550CH2;
W is a leaving group, selected from the group consisting of \u2014Cl, \u2014SO3H and \u2014OPO3H, which is eliminable by a base, a quaternary salt selected from the group consisting of quaternary ammonium, pyridine, carboxypyridinium, methylpyridinium carbonamidopyridinium, and a group of the formula
R3 and R4 each independently are hydrogen or C1-4 alkyl;
U\u2032 is \u03b1,\u03b2-halopropionyl, \u03b1-haloacryloyl, \u03b2-halopropionyl or \u03b1-haloacryloyl;
R\u2032 is hydrogen or C1-4 alkyl;
i and j are integers independent of one another between 0 to 2, and the sum of i and j is not 0;
m and n are integers independent of one another between 2 to 4; and the ring containing the dashed lines represents an optional fused benzene ring.
2. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein R is hydrogen, C1-4 alkyl, C1-4 alkylcarbonyl, phenyl, nitroso or C1-4 alkyl substituted by hydroxyl or sulfo.
3. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein (R1)0-3 and (R2)0-3 each independently are 0 to 3 identical or different groups, and each of R1 and R2 independently is selected from the group consisting of hydrogen, sulfo, C1-4 alkyl and C1-4 alkoxy.
4. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein B and B\u2032 each independently are \u2014SO2\u2014 or \u2014CONH\u2014(CH2)k\u2014SO2\u2014.
5. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein Z and Z\u2032 each independently are selected from the group consisting of \u2014SO2\u2014U, \u2014CONH\u2014(CH2)d\u2014SO2\u2014U and \u2014N(R\u2032)\u2014U\u2032.
6. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a pyrazolone component of the following formula,
wherein, G is C1-3 alkyl, carboxyl or C2-5 alkylcarboxyl; and R5 and R6 each independently are selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, nitro, cyano, C1-4 alkyl and C1-4 alkoxy.
7. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a benzene component of the following formula,
wherein, (R7)0-3 is 0 to 3 identical or different groups, and each of R7 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl.
8. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a naphthalene component selected from the group consisting of
wherein, R12 is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, acetylamino, ureido, C1-4 alkyl and C1-4 alkoxy.
9. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a monoazo component selected from the group consisting of
wherein, (R7)0-3 is 0 to 3 identical or different groups, and each of R7 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl;
R8 is hydrogen, C1-4 alkyl, C2-4 alkylcarboxyl or C1-4 alkyl substituted by hydroxyl, cyano, carboxyl, sulfo, methoxycarbonyl, ethoxycarbonyl or acetoxy;
R11 is hydrogen, C1-4 alkyl, unsubstituted or substituted phenyl by halogen, hydroxyl, carboxyl, sulfo, nitro, C1-4 alkyl or C1-4 alkoxy, or C1-4 alkyl substituted by hydroxyl, carboxyl, cyano or sulfo;
R12 is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, acetylamino, ureido, C1-4 alkyl and C1-4 alkoxy; and the ring containing the dashed lines represents an optional fused benzene ring.
10. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a disazo component selected from the group consisting of
wherein, (R7)0-3 is 0 to 3 identical or different groups, and each of R7 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl;
(R9)0-3 is 0 to 3 identical or different groups, and each of R9 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl;
(R10)0-3 is 0 to 3 identical or different groups, and each of R10 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl;
R11 is hydrogen, C1-4 alkyl, unsubstituted or substituted phenyl by halogen, hydroxyl, carboxyl, sulfo, nitro, C1-4 alkyl or C1-4 alkoxy, or C1-4 alkyl substituted by hydroxyl, carboxyl, cyano or sulfo;
R12 is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, acetylamino, ureido, C1-4 alkyl and C1-4 alkoxy;
R13 is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, acetylamino, uredio, C1-4 alkyl and C1-4 alkoxy; and the ring containing the dashed lines represents an optional fused benzene ring.
11. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a polyazo component of the following formula,
wherein, (R7)0-3 is 0 to 3 identical or different groups, and each of R7 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl;
R11 is hydrogen, C1-4 alkyl, unsubstituted or substituted phenyl by halogen, hydroxyl, carboxyl, sulfo, nitro, C1-4 alkyl or C1-4 alkoxy, or C1-4 alkyl substituted by hydroxyl, carboxyl, cyano or sulfo;
p is 2 or 3; and the ring containing the dashed lines represents an optional fused benzene ring.
12. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a polyazo component selected from the group consisting of
wherein, (R7)0-3 is 0 to 3 identical or different groups, and each of R7 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl;
R11 is hydrogen, C1-4 alkyl, unsubstituted or substituted phenyl by halogen, hydroxyl, carboxyl, sulfo, nitro, C1-4 alkyl or C1-4 alkoxy, or C1-4 alkyl substituted by hydroxyl, carboxyl, cyano or sulfo;
R12 is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, acetylamino, ureido, C1-4 alkyl and C1-4 alkoxy;
R13 is selected from the group consisting of hydrogen, halogen, hydroxyl, carboxyl, sulfo, amino, nitro, cyano, acetylamino, uredio, C1-4 alkyl and C1-4 alkoxy; and
(R14)0-3 is 0 to 3 identical or different groups, and each of R14 independently is selected from the group consisting of halogen, hydroxyl, carboxyl, sulfo, nitro, cyano, C1-4 alkyl, C1-4 alkoxy, C2-6 alkoxycarbonyl, carbamoyl, C2-5 alkanoylamino and C2-5 alkylcarboxyl.
13. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a pyrazolone component of the following formula,
14. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a naphthalene component of the following formula,
15. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a monoazo component selected from the group consisting of
wherein, s, t and v are integers independent of one another between 0 to 2, and s+t+v is an integer between 0 to 3; and
x and y are integers independent of one another between 0 to 2, and x+y is an integer between 0 to 3.
16. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a disazo component selected from the group consisting of
and wherein x and y are integers independent of one another between 0 to 2, and x+y is an integer between 0 to 3.
17. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein E1 and E2 each independently are a metal complex azo component of the following formula,
18. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein the reactive dyestuff of the formula (I) is a compound of the following formula (61),
19. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein the reactive dyestuff of the formula (I) is a compound of the following formula (66),
20. The reactive dyestuff with an N,N-dialkylamino bridge group as claimed in claim 1, wherein the reactive dyestuff of the formula (I) is a compound of the following formula (67),
21. A dyestuff with an N,N-dialkylamino bridge group of the following formula (70),

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1-20. (canceled)
21. A material for sealing off cavities between two structural components, comprising an intumescent mat, wherein the intumescent mat is completely sealed in a polymeric film and the interior bounded by the polymeric film and containing the intumescent mat is evacuated.
22. The material of claim 21 wherein the two structural components are formed of materials having different thermal characteristics or of the same material but have different temperatures.
23. The material of claim 21 wherein said intumescent mat comprises an expandable mica, a silicate and an organic binder.
24. The material of claim 23 wherein the intumescent mat comprises the organic binder in an amount of from 2 to 15 wt %, based on the overall weight of the intumescent mat.
25. The material of claim 23 wherein the silicate is an aluminum silicate.
26. The material of claim 23 wherein the expandable mica is vermiculite.
27. The material of claim 21 consisting of an intumescent mat.
28. The material of claim 21 being a composite mat, said composite mat in addition to an intumescent mat comprising a fibrous mat of oxidic fibers, wherein said intumescent mat and said fibrous mat of oxidic fibers are each sheet bodies joined together at their major surfaces.
29. The material of claim 28 wherein the composite mat comprises a plurality of successive plies of one intumescent mat and one fibrous mat of oxidic fibers.
30. The material of claim 21 formed as a multi-ply composite material comprising two or more intumescent mats with interplies between any two directly successive intumescent mats to mechanically stabilize said multi-ply composite material.
31. The material of claim 30 wherein said interplies are formed of sieves or perforated sheets.
32. The material of claim 21 wherein plastics used for the polymeric film comprise one or more polyamides or mixtures of one or more polyamides with polyethylene andor polypropylene.
33. The material of claim 21 wherein the polymeric film is textured on one side thereof.
34. A method of sealing off cavities between two structural components by using a material of claim 21, which method comprises devacuating the material after insulation in the cavity between the structural components.
35. The method of claim 34 wherein the material is devacuated by puncturing or incising the polymeric film or by elevating the temperature to a value at which the polymeric film burns away.
36. A process for the production of nitric acid, said process comprising catalytic combustion of ammonia on a platinum gauze, which bears on a catalyst bed for the depletion of nitrous oxide from the reaction gas mixture, wherein the catalyst bed is positioned in a catalyst cage which is arranged concentrically inside a reactor comprising the material of claim 21 while keeping an annular gap, and wherein the material for sealing the annular gap is arranged between the catalyst cage and the outer boundary of the annular gap.
37. The process of claim 36, wherein the outer boundary of the annular gap is a metallic apron.
38. The process of claim 36, wherein the outer boundary of the annular gap is the inside wall of the reactor.
39. A process for the thermal insulation of the outside wall of a tube inside which a burner is arranged, wherein the material of claim 21 is applied to the outside wall of an auxiliary tube which has a smaller diameter than the tube to be isolated, which is inserted into the tube to be isolated.
40. A process for the production of sulfur trioxide, said process comprising oxidation of sulfur dioxide in a hurdling contact reactor on a catalyst which contains vanadium pentoxide and which is placed in two, three, four or more hurdlings arranged on top of each other with an interstage cooling between two successive hurdlings, wherein the material of claim 21 is employed for sealing the gap between the outer boundary of the hurdlings and the inner wall of the hurdling contact reactor.