1461100076-2b062ecf-b6b5-4c3a-a73e-c5080c34fb0a

I claim:

1. A sealing apparatus for sealing spaced-apart produts which are enclosed by a film comprising:
a frame, the frame providing a path along which spaced-apart products can move,
a sealing support structure mounted on the frame for movement along said path,
upper and lower sealing units mounted on the sealing support structure for movement toward and away from each other in a direction which is generally perpendicular to said path,
means for moving the upper and lower sealing units toward each other to close the sealing units whereby a film between the sealing units can be sealed and for moving the upper and lower sealing units away from each to open the sealing units, and
means for reciprocating the sealing support along said path between first and second positions.
2. The apparatus of claim 1 in which the sealing support structure is mounted on the frame for linear reciprocating movement along said path.
3. The apparatus of claim 2 in which the upper and lower sealing units are mounted on the sealing support structure for linear movement toward and away from each other.
4. The apparatus of claim 1 in which the upper and lower sealing units are mounted on the sealing support structure for linear movement toward and away from each other.
5. The apparatus of claim 1 in which said means for moving the upper and lower sealing units includes a servo motor.
6. The apparatus of claim 5 in which the means for moving the upper and lower sealing units includes a belt drive which is driven by said servo motor.
7. The apparatus of claim 1 in which said means for reciprocating the sealing support structure includes a crank arm rotatably mounted on the frame and a link connecting the crank arm and the sealing support structure.
8. The apparatus of claim 7 including a servo motor for rotating the crank arm.
9. The apparatus of claim 7 including a linear bearing between the sealing support structure and the frame for supporting the sealing support structure for linear movement along said path.
10. The apparatus of claim 9 including a linear guide on the sealing support structure, the upper and lower sealing units being mounted on the linear guide for linear movement toward and away from each other.
11. The apparatus of claim 7 including a linear guide on the sealing support structure and linear bearings on the upper and lower sealing units for supporting the sealing units for linear movement along said path.
12. The apparatus of claim 1 including a linear guide on the frame and a linear bearing on the sealing support structure for supporting the sealing support structure for linear movement along said path.
13. The apparatus of claim 1 in which said means for reciprocating the sealing support structure includes a belt drive mounted on the frame and connected to the sealing support structure.
14. The structure of claim 1 including a linear guide on the frame which extends in the direction of said path and a linear bearing on the sealing support structure for supporting the sealing support structure for linear movement along said path.
15. The apparatus of claim 1 including a pair of linear guides on the sealing support structure which extend generally perpendicularly to said path, a pair of bearings on each of the upper and lower sealing units slidably mounted on the linear guides for linear movement toward and away from each other.
16. The apparatus of claim 15 including upper and lower cross members connected to said pair of linear guides, and means for moving said upper and lower cross members and said linear guides in a direction which is generally perpendicular to said path.
17. The apparatus of claim 16 in which said linear guides are slidably mounted in said sealing support structure.
18. The apparatus of claim 18 in which said means for moving includes a threaded shaft connected to one of the upper and lower cross members and extending through said sealing support structure whereby rotation of the threaded shaft moves said upper and lower cross members and said linear guides relative to said sealing support structure.
19. The apparatus of claim 16 including upper pulleys rotatably mounted on the upper cross member and lower pulleys rotatably mounted on the lower cross member, a pair of drive belts extending over the upper and lower pulleys, and means for rotating the upper or lower pulleys to move the drive belts, the upper and lower sealing units being connected to the drive belts for movement with the drive belts.
20. The apparatus of claim 15 including upper pulleys rotatably mounted on the upper cross member and lower pulleys rotatably mounted on the lower cross member, a pair of drive belts extending over the upper and lower pulleys, and means for rotating the upper or lower pulleys to move the drive belts, the upper and lower sealing units being connected to the drive belts for movement with the drive belts.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A machine device comprising:
a cryogenic vessel with coolant,
a heat source,
a hot heat exchanger,
a first stage reversible heat conversion device,
a cold heat exchanger,
a second stage pressure conversion device consisting of at least one expander turbine coupled to an electric generator
2. The device as claimed in claim 1, wherein the heat conversion device is a thermoelectric element
3. The device as claimed in claim 1, wherein the heat conversion device is a thermoacoustic engine coupled to an electric generator
4. The device as claimed in claim 1, wherein the heat conversion device is a Stirling engine coupled to an electric generator
5. The device as claimed in claim 1, wherein the cold heat exchanger serves as an electric coil for the first stage generator
6. The device as claimed in claim 1, wherein the hot heat exchanger serves as an electric coil for the first stage generator
7. The device as claimed in claim 1, wherein an ambient air heat exchanger is introduced between the cold heat exchanger and the second stage pressure conversion device.
8. The device as claimed in claim 7, wherein said ambient air heat exchanger is a no frost multiple pass heat exchanger.
9. The device as claimed in claim 1, wherein the electric generator coils are made of the high temperature superconducting material.
10. The device as claimed in claim 1, wherein the expander turbine casing is made of the material with high thermal conductivity.
11. The device as claimed in claim 1, wherein the coolant is the liquid nitrogen
12. The device as claimed in claim 1, wherein the coolant is the liquid argon
13. The device as claimed in claim 1 wherein said heat source is a person.

1461100066-0e04dc71-e563-461e-a02f-6ba49b2c8212

1. A defined culture system for maintaining human pluripotent stem cells in a substantially undifferentiated state, the defined culture system comprising a defined culture medium and one or both of the polypeptides selected from the group consisting of residues 43 to 378 of SEQ ID NO:1 and residues 45 to 378 of SEQ ID NO:1.
2. The system of claim 1, wherein the defined culture system is capable of maintaining human pluripotent stem cells in a substantially undifferentiated state for at least 20 passages in the absence of feeder cell support.
3. The system of claim 1, wherein the defined culture system is capable of maintaining human pluripotent stem cells in a substantially undifferentiated state for at least 25 passages in the absence of feeder cell support.
4. The system of claim 1, wherein the defined culture system is substantially free of xenogenic contamination with regard to the stem cells.
5. The system of claim 1, wherein the defined culture medium comprises insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEMF12, NaHCO3, and one of TGF\u03b2 and NODAL.
6. The system of claim 1, wherein the defined culture medium consists essentially of insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEMF12, NaHCO3, and one of TGF\u03b2 and NODAL.
7. A cell culture comprising:
undifferentiated human pluripotent stem cells;
a defined cell culture medium suitable for proliferation of the pluripotent stem cells; and
a substrate comprising one or both of the polypeptides selected from the group consisting of residues 43 to 378 of SEQ ID NO:1 and residues 45 to 378 of SEQ ID NO:1.
8. The cell culture of claim 7, wherein the polypeptide is SEQ ID NO:2.
9. The cell culture of claim 7, wherein the-polypeptide is SEQ ID NO:4.
10. The cell culture of claim 7, wherein the substrate is substantially free of xenogenic contamination with regard to the pluripotent stem cells.
11. The cell culture of claim 7, wherein the defined culture medium comprises insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEMF12, NaHCO3, and one of TGF\u03b2 and NODAL.
12. The cell culture of claim 7, wherein the defined culture medium consists essentially of insulin, selenium, transferrin, L-ascorbic acid, FGF2, DMEMF12, NaHCO3, and one of TGF\u03b2 and NODAL.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A display device, comprising:
first electrodes classified into a plurality of groups;
second electrodes respectively provided so as to cross said first electrodes;
a display panel having a plurality of capacitive light emitting elements respectively provided at intersections of said first electrodes and said second electrodes;
a drive circuit that applies a data pulse for light-emitting a selected capacitive light emitting element to the first electrodes in said plurality of groups such that phase differences respectively occur between said plurality of groups;
a number-of-times detector; and
a converter,
said drive circuit comprising:
a first power supply terminal that receives a first power supply voltage;
an inductive element;
a recovering capacitive element;
an application circuit that discharges charges from said recovering capacitive element by a resonance operation of a capacitance of said display panel and said inductive element to raise a potential at a first node, connects said first node and said first power supply terminal to each other, then disconnects said first node and said first power supply terminal from each other, and recovers charges in said recovering capacitive element from said first node through said inductive element by said resonance operation to lower the potential at said first node, to apply to said first node a driving pulse for applying a data pulse to the first electrodes in said plurality of groups;
a potential limiting circuit that limits a quantity of charges recovered in said recovering capacitive element, to limit a potential of said recovering capacitive element so as not to exceed a predetermined value lower than said first power supply voltage; and

a controller,
said converter converting, in order to divide one field into a plurality of sub-fields and discharge said capacitive light emitting element selected for each of the sub-fields to perform gray scale expression, image data corresponding to the one field into image data corresponding to the sub-field,
said number-of-times detector detecting one of a number of times of rise and a number of times of fall of the data pulse applied to said first electrodes for each of the sub-fields on the basis of the image data fed from said converter, and
said controller calculating a ratio of said number of times obtained by said number-of-times detector to one of a maximum number of times the data pulse can rise and a maximum number of times the data pulse can fall in each of the sub-fields, lowering, when said ratio is more than a predetermined ratio value, the potential at said first node to a predetermined voltage value, and then controlling an operation of said application circuit such that said first node is grounded.
2. The display device according to claim 1, wherein said predetermined ratio value is not less than 95%.
3. A method of driving a display device comprising first electrodes classified into a plurality of groups, second electrodes respectively provided so as to cross the first electrodes, and a display panel comprising a plurality of capacitive light emitting elements respectively provided at intersections of the first electrodes and the second electrodes, comprising:
respectively applying a data pulse for light-emitting a selected capacitive light emitting element to the first electrodes in the plurality of groups such that phase differences respectively occur between the plurality of groups,
applying the data pulse comprising:
discharging charges from a recovering capacitive element by a resonance operation of a capacitance of the display panel and an inductive element to raise a potential at a first node, connecting the first node and a first power supply terminal to each other, then disconnecting the first node and the first power supply terminal from each other, and recovering the charges in the recovering capacitive element from the first node through the inductive element by the resonance operation to lower the potential at the first node, to apply to the first node a driving pulse for applying a data pulse to the first electrodes in the plurality of groups; and
limiting the quantity of charges recovered in the recovering capacitive element, to limit a potential of the recovering capacitive element so as not to exceed a predetermined value lower than a first power supply voltage,

said method further comprising:
converting, in order to divide one field into a plurality of sub-fields and discharge the capacitive light emitting element selected for each of the sub-fields to perform gray scale expression, image data corresponding to the one field into image data corresponding to the sub-field;
detecting one of a number of times of rise and a number of times of fall of the data pulse applied to the first electrodes for each of the sub-fields on the basis of the converted image data; and
calculating a ratio of the number of times detected to one of a maximum number of times the data pulse can rise and the maximum number of times the data pulse can fall in each of the sub-fields, lowering, when the ratio is more than a predetermined ratio value, the potential at the first node to a predetermined voltage value, and then grounding the first node.