All The Claims

1. A heat shrink oven for heat shrinking a film wrapping an object, the heat shrink oven comprising:
a housing having a heat treatment chamber for containing steam and for arranging a steam flow pattern,
a transport device for supporting and moving the object through the heat treatment chamber,
at least one steam supply for supplying steam to the heat treatment chamber,
at least a primary steam unit comprising a first and a second steam outlet device comprising one or more outlets for steam, the steam outlet devices connected to the steam supply, the first steam outlet device disposed in the heat treatment chamber along a first side of the transport device and the second steam outlet device disposed along a second side of the transport device, opposite the first side, the outlets directed towards the moving object,
wherein the heat shrink oven further comprises,
a secondary steam system, arranged in the heat treatment chamber, for generating a downward directed steam flow in an area extending between the steam outlet devices, the downward directed steam flow having a lower temperature than the steam supplied from the steam outlet devices, wherein the secondary steam system is arranged to be supplied with steam internal in the heat treatment chamber.
2. The heat shrink oven according to claim 1, wherein the secondary steam system is arranged to supply secondary steam having a temperature of at least 4 degrees Celsius lower than the primary steam flow entering the heat treatment chamber through the outlets.
3. The heat shrink oven according to claim 1, wherein the secondary steam system is arranged to supply secondary steam having a temperature of less than 88 degrees Celsius.
4. The heat shrink oven according to claim 1, wherein the outlets are positioned to supply the primary steam directed generally horizontal.
5. The heat shrink oven according to claim 1, wherein the secondary steam system for generating the downwardly directed steam flow is also arranged to generate a generally upward directed steam flow in the heat treatment chamber in an area outside the area between the opposite fluid outlet devices.
6. The heat shrink oven according to claim 1, wherein the secondary steam system for generating the downwardly directed steam flow comprises a guide allowing the outward guiding of the steam flow.
7. The heat shrink oven according to claim 5, wherein the apparatus comprises a frame received in the heat treatment chamber for mounting the steam outlet devices and wherein the secondary steam system for generating the steam flow surrounds the steam outlet devices mounted to the frame.
8. The heat shrink oven according to claim 1, wherein the secondary steam system for generating the downwardly directed steam flow further comprises steam flow device arranged to generate the downward flow of steam.
9. The heat shrink oven according to claim 1, wherein multiple sets of steam outlet devices are provided along the transport device and wherein the secondary steam system is positioned at the most upstream steam outlet devices or upstream from the most upstream steam outlet devices.
10. The heat shrink oven according to claim 1, wherein the steam outlet device comprises at least three or more outlets in a straight line.
11. The heat shrink oven according to claim 1, wherein the transport device is at least partially received in the heat treatment chamber and the object is a generally cylindrical container for a beverage and the film is a cylindrical envelope.
12. The heat shrink oven according to claim 1, wherein the heat treatment chamber is a closed housing containing the steam, the heat treatment chamber having a tunnel-shaped space, the transport device arranged to carry objects through the tunnel-shaped space.
13. The heat shrink oven according to claim 1, wherein the heat treatment chamber is arranged to allow circulation of the secondary steam flow.
14. The heat shrink oven according to claim 13, wherein the heat treatment chamber and secondary steam system are arranged to maintain the flow of secondary steam continuously.
15. A method of shrinking a heat shrinkable film wrapping an object, the method comprising in a heat treatment chamber of a heat shrink oven:
transporting the upright standing object wrapped with heat shrinkable film through the heat treatment chamber,
applying primary steam onto a side of the film, and
directing secondary steam of lower temperature than the primary in a downward direction onto the object,
the heat treatment chamber containing the steam and allowing circulation of secondary steam.
16. The method according to claim 15, wherein the method comprises maintaining the flow of secondary steam continuously in the heat treatment chamber.
17. The method according to claim 15, wherein the secondary steam is directed onto the object upstream from the application of primary steam.
18. The method according to claim 15, wherein the secondary steam is directed onto the object during application of the primary steam.
19. The method according to claim 15, wherein directing the steam in the downward direction comprises generating a closed flow pattern for steam in the heat treatment chamber, in the downward direction onto the object, in an outwards direction, away from the object, followed by an upward direction and inward direction.
20. The method according to claim 15, wherein applying the steam for heat shrinking a first part of the film comprises applying the steam to a bottom part of the object.
21. The method according to claim 15, wherein the method comprises multiple stages of applying steam to the object being transported through the heat treatment chamber.
22. The method according to claim 15, wherein steam is applied from two opposite sides onto the object carried through the heat treatment chamber.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A plant of soybean variety A1036170, wherein a sample of seed of said variety has been deposited under ATCC Accession No. PTA-121538.
2. A plant part of the plant of claim 1, wherein the plant part comprises at least one cell of said plant.
3. The plant part of claim 2, further defined as pollen, a meristem, a cell, or an ovule.
4. A seed of soybean variety A1036170, wherein a sample of seed of said variety has been deposited under ATCC Accession No. PTA-121538.
5. A method of producing soybean seed, wherein the method comprises crossing the plant of claim 1 with itself or a second soybean plant.
6. The method of claim 5, wherein the method is further defined as comprising crossing the plant of soybean variety A1036170 with a second, distinct soybean plant to produce an F1 hybrid soybean seed.
7. An F1 hybrid soybean seed produced by the method of claim 6.
8. An F1 hybrid soybean plant produced by growing the seed of claim 7.
9. A composition comprising the seed of claim 4 comprised in plant seed growth media, wherein a sample of seed of said variety has been deposited under ATCC Accession No. PTA-121538.
10. The composition of claim 9, wherein the growth media is soil or a synthetic cultivation medium.
11. A plant of soybean variety A1036170, further comprising a single locus conversion, wherein a sample of seed of soybean variety A1036170 has been deposited under ATCC Accession No. PTA-121538.
12. A seed that produces the plant of claim 11.
13. The seed of claim 12, wherein the single locus confers a trait selected from the group consisting of male sterility, herbicide tolerance, insect resistance, pest resistance, disease resistance, modified fatty acid metabolism, abiotic stress resistance, altered seed amino acid composition, site-specific genetic recombination, and modified carbohydrate metabolism.
14. The seed of claim 12, wherein the single locus confers tolerance to an herbicide selected from the group consisting of glyphosate, sulfonylurea, imidazalinone, dicamba, glufosinate, phenoxy proprionic acid, cyclohexanedione, triazine, benzonitrile, PPO-inhibitor herbicides and broxynil.
15. The seed of claim 12, wherein the single locus conversion comprises a transgene.
16. The method of claim 6, wherein the method further comprises:
(a) crossing a plant grown from said F1 hybrid soybean seed with itself or a different soybean plant to produce a seed of a progeny plant of a subsequent generation;
(b) growing a progeny plant of a subsequent generation from said seed of a progeny plant of a subsequent generation and crossing the progeny plant of a subsequent generation with itself or a second plant to produce a progeny plant of a further subsequent generation; and
(c) repeating steps (a) and (b) using said progeny plant of a further subsequent generation from step (b) in place of the plant grown from said F1 hybrid soybean seed in step (a), wherein steps (a) and (b) are repeated with sufficient inbreeding to produce an inbred soybean plant derived from the soybean variety A1036170.
17. The method of claim 16, comprising crossing said inbred soybean plant derived from the soybean variety A1036170 with a plant of a different genotype to produce a seed of a hybrid soybean plant derived from the soybean variety A1036170.
18. A method of producing a commodity plant product comprising collecting the commodity plant product from the plant of claim 1.
19. The method of claim 18, wherein the commodity plant product is protein concentrate, protein isolate, grain, soybean hulls, meal, flour, or oil.
20. A soybean commodity plant product produced by the method of claim 19, wherein the commodity plant product comprises at least one cell of soybean variety A1036170.
21. A soybean plant produced by transforming the plant of claim 1 with a transgene, or a selfed progeny thereof comprising the transgene.

1. A method of rendering an application view and connecting a component’s behavior to events on the user interface, the method comprising;
registering a view object as a listener on a document in data model that correspond to events on the computer display;
instantiating and associating an object that contains the computational logic of a component with the view object; and
identifying the appropriate references related to the component in the data model that correspond to events on the user interface and registering the components events.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method of determining an initial concentration (Q0) of a target nucleic acid template in a sample, the method comprising the steps of:
a) amplifying said target nucleic acid template and a plurality of standard templates, each of said standard templates present at a different, known copy number, in a nucleic acid amplification reaction regimen comprising a plurality of cycles of primer annealing, primer elongation and strand dissociation, wherein said reaction is performed using a reaction mixture comprising said target nucleic acid template and said plurality of standard templates, and wherein the efficiency of amplification, E, is similar for said plurality of standards and said target template;
b) measuring, at plural cycles of said amplifying regimen, fluorescent signals from said target and each of said plurality of standard templates after nucleic acid species in an aliquot taken from the reaction mixture are separated by capillary electrophoresis, wherein said measuring generates a set of measurements for each said template;
c) estimating a cycle threshold (Ct) value for each of said plurality of standard templates and said target template, wherein the Ct value is estimated by a method comprising the steps, for each set of measurements for each said template, of:
i) compiling a candidate list of all sets of consecutive signal measurements with cardinality 3 or greater;
ii) removing those signal measurements from said candidate list for which peak area measurement is less than peak area measurement at the previous cycle;
iii) computing a best-fitting line by linear regression for each set of measurements, said line representing a log-linear amplification curve described by equation (1):
log(measured value)=C0+EC

wherein C is the cycle number, C0 is the X intercept and E is the slope of the line;

iv) computing a fitness score for each of said target nucleic acid and said plurality of standards, wherein the fitness score is computed as a weighted sum of the correlation coefficient of the linear regression computed in step (iii), the cardinality of the candidate data set, and proximity to the fluorescence threshold;
v) selecting the set with the highest fitness score as the best set for each of said target nucleic acid and said plurality of standards; and
vi) computing Ct from the best set from each of said target nucleic acid and said plurality of standards, wherein Ct is computed by:
A) choosing a threshold value for log(measured value) of equation (1)
B) solving equation (1) for C when log(measured value) equals the chosen threshold value, C0 equals the X intercept determined in step (c)(iii) and E equals the slope determined in step (c)(iii);
C) setting Ct equal to the solved value of C;
d) generating a standard curve by plotting Ct values estimated in step (c) on the y axis for each of said plurality of said standard templates versus the log of said known copy number on the x axis for each said standard template; and
e) calculating an initial concentration (Q0) for said target nucleic acid template by solving the equation
C

t
\u2061

(
target
)
=
I
–

log
\u2061

(

Q
0

)
log
\u2061

(

E
R

)
for Q0, wherein I is the X intercept of said standard curve and ER is the efficiency obtained from said standard curve.
2. The method of claim 1 wherein said measurements of step (b) are entered into a computer-readable physical memory, and wherein steps (c)-(e) are performed by a computer processor executing instructions, encoded on a computer-readable physical memory, for performing such steps.
3. The method of claim 1 wherein each of said standard templates and said target nucleic acid are amplified by the same pair of oligonucleotide primers.
4. The method of claim 1 wherein said measuring step (b) is performed on a plurality of aliquots taken from said reaction mixture at respective plural cycles during said amplifying regimen.
5. A non-transitory computer-readable physical storage medium comprising instructions, that when executed by a processor, cause the processor to perform a procedure comprising the steps of:
a) receiving a plurality of measurements obtained from a plurality of standard templates;
b) receiving a plurality of measurements obtained from a target template;
c) estimating a cycle threshold (CO value for each of said plurality of standard templates and said target template, wherein the Ct value is estimated by a method comprising the steps, for each set of measurements for each said template, of:
i) compiling a candidate list of all sets of consecutive signal measurements with cardinality 3 or greater;
ii) removing those signal measurements from said candidate list for which peak area measurement is less than peak area measurement at the previous cycle;
iii) computing a best-fitting line by linear regression for each set of measurements, said line representing a log-linear amplification curve described by equation (1):
log(measured value)=C0+EC

wherein C is the cycle number, C0 is the X intercept and E is the slope of the line;

iv) computing a fitness score for each of said target nucleic acid and said plurality of standards, wherein the fitness score is computed as a weighted sum of the correlation coefficient of the regression computed in step (iii), the cardinality of the candidate data set, and proximity to the fluorescence threshold;
v) selecting the set with the highest fitness score as the best set for each of said target nucleic acid and said plurality of standards; and
vi) computing Ct from the best set from each of said target nucleic acid and said plurality of standards, wherein Ct is computed by:
A) choosing a threshold value for log(measured value) of equation (1)
B) solving equation (1) for C when log(measured value) equals the chosen threshold value, C0 equals the X intercept determined in step (c)(iii) and E equals the slope determined in step (c)(iii);
C) setting Ct equal to the solved value of C;
d) generating a standard curve by plotting Ct values estimated in step (c) on the y axis for each of said plurality of said standard templates versus the log of said known copy number on the x axis for each said standard template; and
e) calculating an initial concentration (Q0) for said target nucleic acid template by solving the equation
C

t
\u2061

(
target
)
=
I
–

log
\u2061

(

Q
0

)
log
\u2061

(

E
R

)
for Q0, wherein I is the X intercept of said standard curve and ER is the efficiency obtained from said standard curve.
6. A system comprising a computer processor, and instructions that cause the processor to perform a procedure comprising the steps of:
a) receiving a plurality of measurements obtained from a plurality of standard templates;
b) receiving a plurality of measurements obtained from a target template;
c) estimating a cycle threshold (Ct) value for each of said plurality of standard templates and said target template, wherein the Ct value is estimated by a method comprising the steps, for each set of measurements for each said template, of:
i) compiling a candidate list of all sets of consecutive signal measurements with cardinality 3 or greater;
ii) removing those signal measurements from said candidate list for which peak area measurement is less than peak area measurement at the previous cycle;
iii) computing a best-fitting line by linear regression for each set of measurements, said line representing a log-linear amplification curve described by equation (1):
log(measured value)=C0+EC

wherein C is the cycle number, C0 is the X intercept and E is the slope of the line;

iv) computing a fitness score for each of said target nucleic acid and said plurality of standards, wherein the fitness score is computed as a weighted sum of the correlation coefficient of the regression computed in step (iii), the cardinality of the candidate data set, and proximity to the fluorescence threshold;
v) selecting the set with the highest fitness score as the best set for each of said target nucleic acid and said plurality of standards; and
vi) computing Ct from the best set from each of said target nucleic acid and said plurality of standards, wherein Ct is computed by:
A) choosing a threshold value for log(measured value) of equation (1)
B) solving equation (1) for C when log(measured value) equals the chosen threshold value, C0 equals the X intercept determined in step (c)(iii) and E equals the slope determined in step (c)(iii);
C) setting Ct equal to the solved value of C;
d) generating a standard curve by plotting Ct values estimated in step (c) on the y axis for each of said plurality of said standard templates versus the log of said known copy number on the x axis for each said standard template; and
e) calculating an initial concentration (Q0) for said target nucleic acid template by solving the equation
C

t
\u2061

(
target
)
=
I
–

log
\u2061

(

Q
0

)
log
\u2061

(

E
R

)
for Q0, wherein I is the X intercept of said standard curve and ER is the efficiency obtained from said standard curve.