All The Claims

1. A FPCB cable and cable connector assembly comprising:
a conductive line unit comprising a signal line;
a terminal, a portion of the signal line being exposed at the terminal;
a terminal protector extending from a side of the conductive line unit and surrounding the terminal; and
a cable connector coupled to the terminal.
2. The FPCB cable and cable connector assembly of claim 1, wherein the cable connector is between the terminal protector and the conductive line unit.
3. The FPCB cable and cable connector assembly of claim 1, wherein the terminal protector is integrally formed with an insulating layer of the conductive line unit.
4. The FPCB cable and cable connector assembly of claim 3, wherein the terminal protector comprises a conductive layer insulated from the signal line.
5. The FPCB cable and cable connector assembly of claim 1, wherein the cable connector comprises an actuator configured to fix the FPCB cable to the cable connector.
6. The FPCB cable and cable connector assembly of claim 5, wherein the FPCB cable comprises a flexible printed circuit board.
7. The FPCB cable and cable connector assembly of claim 5, wherein the cable connector has a groove formed at a side surface thereof and opens in a direction away from the terminal.
8. An FPCB cable and cable connector assembly comprising:
a conductive line unit comprising a signal line;
a terminal, a portion of the signal line being exposed at the terminal;
a terminal protector extending from a side of the conductive line unit and surrounding the terminal, the signal line extending along at least a portion of the terminal protector; and
a flexible printed circuit board cable connector,
wherein the terminal protector is configured to surround the cable connector.
9. The FPCB cable and cable connector assembly of claim 8, wherein the cable connector is between the terminal protector and the conductive line unit.
10. The FPCB cable and cable connector assembly of claim 8, wherein the terminal protector is integrally formed with an insulating layer of the conductive line unit.
11. The FPCB cable and cable connector assembly of claim 8, wherein the terminal has a concave portion or a convex portion configured to be coupled to the cable connector.
12. The FPCB cable and cable connector assembly of claim 8, wherein the cable connector comprises an actuator configured to fix the flexible printed circuit board cable to the cable connector.
13. The FPCB cable and cable connector assembly of claim 8, wherein the cable connector has a groove at a same side surface at which the terminal is configured to be fixed to the cable connector.
14. An FPCB cable comprising:
a conductive line unit comprising a signal line;
a terminal exposing a portion of the signal line; and
a terminal protector extending from a side of the conductive line unit and surrounding the terminal.
15. The FPCB cable of claim 14, wherein the terminal further has a concave portion or a convex portion.
16. An FPCB cable comprising:
a conductive line unit comprising a plurality of signal lines;
at least two terminals, each of the terminals exposing a portion of the signal lines; and
at least two terminal protectors coupled to respective ones of the terminals, extending from a side of the conductive line unit, and surrounding the respective ones of the terminals,
wherein the signal lines extend along at least a portion of each of the terminal protectors.
17. The FPCB cable of claim 16, wherein the signal lines have substantially the same length as each other.
18. The FPCB cable of claim 17, wherein the terminal protectors comprise a first terminal protector and a second terminal protector, and wherein an innermost one of the signal lines extending along the first terminal protector is an outermost one of the signal lines extending along the second terminal protector.
19. The FPCB cable of claim 16, wherein the exposed portions of the signal lines each comprise an endpin connector having a concave shape or a convex shape.
20. The FPCB cable of claim 19, wherein the endpin connectors at one of the terminals are arranged adjacent to each other in a zigzag manner.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method comprising:
receiving, by a network device, a request for an on-demand streaming service from a program source device, wherein the on-demand streaming service provides on-demand publishing of programs originating from program source devices, and wherein the publishing includes streaming of the programs via the Internet to users;
obtaining, by the network device, configuration data based on the request;
turning on, by the network device, a virtual live slicer server based on the request;
transmitting, by the network device, the configuration data to the virtual live slicer server; and
transmitting, by the network device, a network address of the virtual live slicer server to the program source device.
2. The method of claim 1, further comprising:
capturing, by the program source device, the program, subsequent to receiving the network address; and
streaming, by the program source device, the program to the network device.
3. The method of claim 2, further comprising:
receiving, by the virtual live slicer server, a program stream from the program source device; and
encoding, by the virtual live slicer server, the program stream.
4. The method of claim 3, further comprising:
transmitting, by the virtual live slicer server, an encoded program stream to a cloud device;
transcoding, by the cloud device, the encoded program stream; and
publishing, by the cloud device, a transcoded program stream.
5. The method of claim 2, further comprising:
monitoring, by the virtual live slicer server, during the receiving, a state of a streaming session associated with a program stream from the program source device;
determining, by the virtual live slicer server, whether the state is inactive; and
disconnecting, by the virtual live slicer server, a connection between the virtual live slicer server and the program source device based on determining that the state is inactive.
6. The method of claim 1, wherein the turning on comprises:
load balancing, by the network device, physical resources of network devices on which a virtual live slicer server farm operates.
7. The method of claim 6, wherein the turning on comprises:
causing a virtual live slicer server application, which is installed on one of the network devices, to execute in accordance with the configuration data.
8. The method of claim 6, further comprising:
continuously updating a file that stores utilization of the physical resources; and
using the file to select one of the network devices having a lowest physical resource utilization among the network devices to turn on an instance of a virtual live slicer server.
9. A device comprising:
a communication interface;
a memory, wherein the memory stores instructions; and
a processor, wherein the processor executes the instructions to:
receive, via the communication interface, a request for an on-demand streaming service from a program source device, wherein the on-demand streaming service provides on-demand publishing of programs originating from program source devices, and wherein the publishing includes streaming of the programs via the Internet to users;
obtain configuration data based on the request;
turn on a virtual live slicer server based on the request;
transmit, via the communication interface, the configuration data to the virtual live slicer server; and
transmit, via the communication interface, a network address of the virtual live slicer server to the program source device.
10. The device of claim 9, wherein the request includes an identifier, and wherein the processor further executes the instructions to:
access a database, wherein the database includes identifiers of at least one of program source users or program source devices and configuration data mapped to the identifiers;
compare the identifier included in the request to the identifiers included in the database;
determine whether to validate the request based on a comparison of the identifier included in the request and the identifiers included in the database; and
select the configuration data mapped to the identifier based on a determination that the request is valid.
11. The device of claim 9, wherein the configuration data includes three or more of a protocol setting, a video setting, an audio setting, a geo-blocking setting, or an advertisement setting.
12. The device of claim 9, wherein the processor further executes the instructions to:
determine that the program source device does not have an account for the on-demand streaming service; and wherein when obtaining the configuration data, the processor further executes the instructions to:
select one or more default values to use as the configuration data based on a determination that the program source device does not have the account.
13. The device of claim 9, wherein the processor further executes the instructions to:
receive, via the communication interface, configuration data from the program source device; and
transmit, via the communication interface, the configuration data received from the program source device to the virtual live slicer server.
14. The device of claim 9, wherein the processor further executes the instructions to:
load balance physical resources of network devices on which a virtual live slicer server farm operates.
15. The device of claim 14, wherein, when turning on the virtual live slicer server, the processor further executes the instructions to:
cause a virtual live slicer server application, which is installed on one of the network devices, to execute, on-demand, in accordance with the configuration data.
16. A non-transitory storage medium comprising instructions executable by a processor of a computational device, which when executed by the processor, cause the computational device to:
receive a request for an on-demand streaming service from a program source device, wherein the on-demand streaming service provides on-demand publishing of programs originating from program source devices, and wherein the publishing includes streaming of the programs via the Internet to users;
obtain configuration data based on the request;
turn on a virtual live slicer server based on the request;
transmit the configuration data to the virtual live slicer server; and
transmit a network address of the virtual live slicer server to the program source device.
17. The non-transitory storage medium of claim 16, wherein the request includes an identifier, and the non-transitory storage medium further comprising instructions, which when executed by the processor, cause the computational device to:
access a database, wherein the database includes identifiers of at least one of program source users or program source devices and configuration data mapped to the identifiers;
compare the identifier included in the request to the identifiers included in the database;
determine whether to validate the request based on a comparison of the identifier included in the request and the identifiers included in the database; and
select the configuration data mapped to the identifier based on a determination that the request is valid.
18. The non-transitory storage medium of claim 16, further comprising instructions, which when executed by the processor, cause the computational device to:
determine that the program source device does not have an account for the on-demand streaming service; and wherein when obtaining the configuration data, the instructions further comprise instructions to:
select one or more default values to use as the configuration data based on a determination that the program source device does not have the account.
19. The non-transitory storage medium of claim 16, further comprising instructions, which when executed by the processor, cause the computational device to:
load balance physical resources of network devices on which a virtual live slicer server farm operates based on a lowest usage selection and queue process.
20. The non-transitory storage medium of claim 19, further comprising instructions, which when executed by the processor, cause the computational device to:
cause a virtual live slicer server application, which is installed on one of the network devices, to execute, on-demand, in accordance with the configuration data.

We claim:

1. A nucleic acid with a 5 end and a 3 end comprising a first functional nucleotide sequence and a scissile strand topoisomerase I cleavage motif sequence, wherein the scissile strand topoisomerase I cleavage motif sequence is located 3 to the first functional nucleotide sequence and provides a scissile strand topoisomerase I cleavage site that is not more than 10 bases from the 3 end of the nucleic acid.
2. The nucleic acid of claim 1, wherein the scissile strand topoisomerase cleavage motif sequence is selected from the group consisting of: CCCTT and TCCTT.
3. The nucleic acid of claim 1, wherein the first functional nucleotide sequence is selected from the group consisting of: a prokaryotic promoter sequence, a eukaryotic promoter sequence, a viral promoter sequence, a mutational sequence, a polypeptide tag encoding sequence, a nucleic acid tag sequence, a terminator sequence, a fusible protein encoding sequence, a radioactively labeled nucleotide sequence, a chemically labeled nucleotide sequence and an intronic sequence.
4. An adaptor comprising a first nucleic acid with a 5 end and a 3 end and comprising a scissile strand topoisomerase I cleavage motif having a 5 motif sequence contiguous with a 3 motif terminal nucleotide, said 3 motif terminal nucleotide being contiguous with a palindromic sequence of not less than two nucleotides nor more than 10 nucleotides and said palindromic sequence being contiguous with a 3 end nucleotide that is complementary to the 3 motif terminal nucleotide of the scissile strand topoisomerase I cleavage motif.
5. The adaptor of claim 4 further comprising a second nucleic acid having a 5 end sequence that is complementary to the 5 motif sequence of the scissile strand topoisomerase I cleavage motif.
6. The first nucleic acid of the adaptor of claim 4, wherein the 3 motif terminal nucleotide of the scissile strand topoisomerase I cleavage motif is T and the 5 motif sequence of the scissile strand topoisomerase I cleavage motif is selected from the group consisting of CCCT and TCCT.
7. The first nucleic acid of the adaptor of claim 4 further comprising a restriction endonuclease site located 5 to the scissile strand topoisomerase I cleavage motif.
8. The first nucleic acid of the adaptor of claim 4 further comprising a 5 end sequence that is complementary to the 5-overhang of a restriction endonuclease site.
9. The first nucleic acid of claim 7 or claim 8, wherein the restriction endonuclease is selected from the group consisting of: BamH I, Bgl II, Cla I, Dde I, Eae I, Eag I, EcoR I, Hind III, Kas I, Mbo I, Mlu I, Nco I, Nde I, Nhe I, Not I, PaeR7 I, Sal I, Sau3A, Spe I, Sty I, Xba I, Xho I and Xma I.
10. The first nucleic acid of the adaptor of claim 4, further comprising a first functional nucleotide sequence selected from the group consisting of: a prokaryotic promoter sequence, a eukaryotic promoter sequence, a viral promoter sequence, a mutational sequence, a polypeptide tag encoding sequence, a nucleic acid tag sequence, a terminator sequence, a fusible protein encoding sequence, a radioactively labeled nucleotide sequence, a chemically labeled nucleotide sequence and an intronic sequence.
11. A method for joining an adaptor sequence to a target nucleic acid sequence comprising:
providing a nucleic acid adaptor of claim 5,
providing a target nucleic acid with a one base 3 overhang nucleotide that is complementary to the 3 motif terminal nucleotide of the scissile strand topoisomerase cleavage motif, and
incubating the nucleic acid adaptor with the target nucleic acid in the presence of a topoisomerase I activity,
thereby joining the adaptor sequence to the target nucleic acid sequence.
12. The method of claim 11, wherein the first nucleic acid of the adaptor of claim 5 further comprises a functional nucleotide sequence that is 5 to the scissile strand topoisomerase I cleavage motif.
13. The method of claim 12, wherein the functional nucleotide sequence is selected from the group consisting of: a prokaryotic promoter sequence, a eukaryotic promoter sequence, a viral promoter sequence, a mutational sequence, a polypeptide tag encoding sequence, a nucleic acid tag sequence, a terminator sequence, a fusible protein encoding sequence, a radioactively labeled nucleotide sequence, an intronic sequence.
14. The method of claim 12, wherein the functional nucleotide sequence is a phage promoter selected from the group consisting of: an SP6 promoter, a T3 promoter and a T7 promoter.
15. The method of claim 11, further comprising the step of amplifying the joined product.
16. The method of claim 15, wherein the joined product is amplified by a polymerase chain reaction utilizing a first primer specific to the nucleic acid adaptor and a second primer specific to the target nucleic acid sequence.
17. The method of claim 11, wherein the target nucleic acid is generated by a polymerase chain reaction of a target genomic or a target cDNA sequence with a 5 sense strand primer and a 3 anti-sense strand primer.
18. The method of claim 17, wherein the adaptor provides a functional nucleotide sequence that is a promoter sequence and further comprising the steps of preparing at least two separate amplification reactions from the joined product comprising:
a first amplification reaction with 3 anti-sense strand primer and a first adaptor primer; and
a second amplification reaction with a 5 sense strand primer and a second adaptor primer, wherein the first adaptor primer comprises a sequence in the first nucleic acid of the adaptor and the second adaptor primer comprises a sequence in the second nucleic acid of the adaptor.
19. The method of claim 18 further comprising the step of isolating the product of either the first amplification reaction or the second amplification reaction.
20. The method of claim 19 further comprising contacting the amplification product with an RNA polymerase activity which recognizes said promoter sequence.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

What is claimed is:

1. An intubating laryngoscope system providing for ready interchange of blades, for video display, and for convenience in use comprising:
a handle to be held by an anesthesiologist;
a blade having a surface configured to enable the anesthesiologist to elevate the tongue of a patient, a proximal end, and a distal end;
a hinge-like joinder separably to join said handle and said blade, said joinder having a first portion on said handle and a second portion on said proximal end of said blade, one of said portions having a rod and the other portion having a hook, whereby in an engaged orientation the hook and the rod are engaged, and in a released configuration they may be separated, the handle and blade being rigidly attached to one another in the engaged orientation;
a flexible cable extending from said handle having a fixed end fixed to the handle, and a free end;
a guide tube attached to said blade, extending from near the proximal end of the blade toward its distal end, said guide tube including an enlarged a funnel-like entry portion to facilitate the entry of said flexible cable into said guide tube, the length of said flexible cable being such that when the handle and blade are in the engaged orientation, the flexible cable is disposed in a predetermined position in the blade;
a camera;
a light source;
a light-transmitting fiber optic in said flexible cable;
image-transmitting means in said flexible cable;
a second cable extending from said handle;
a video receiver connected to said second cable receptive to signals from said camera; and
a video screen receptive to said video receiver to display images transmitted from said camera.
2. A system according to claim 1 in which said camera is disposed in a chamber in a said standard handle, and said image-transmitting flexible cable comprises a coherent fiber optic bundle having one end exposed to a field of view and a second end exposed to said camera.
3. A system according to claim 1 in which said light source is separate from the handle, and in which said light-transmitting fiber optic is adapted to receive light from said light source to be transmitted to the field of view.
4. A system according to claim 3 in which said camera is disposed in a chamber in said standard handle, and said flexible cable includes a coherent fiber optic bundle having one end exposed to a field of view and a second end exposed to said camera.
5. A system according to claim 1 in which said camera is mounted to the distal end of the flexible cable, and the image-transmitting means is a lead which conveys image data through said handle to said video receiver.
6. A system according to claim 1 in which said light source is a battery and bulb combination in said handle to provide light to said light-transmitting means.
7. An intubating laryngoscope system enabling relay interchange of blades, video display, and convenient use, comprising:
a handle to be held by an anesthesiologist;
a blade having a surface configured to enable the anesthesiologist to elevate the tongue of a patient, a proximal end, and a distal end;
a hinge-like joinder separably to join said handle and said blade, said joinder having a first portion on said handle and a second portion on said proximal end of said blade, one of said portions having a rod and the other portion having a hook, whereby in an engaged orientation the hook and the rod are engaged, and in a released configuration they may be separated, the handle and blade being rigidly attached to one another in the engaged orientation;
a flexible cable extending from said handle having a fixed end fixed to the handle, and a free end;
a guide tube attached to said blade, extending from near the proximal end of the blade toward its distal end, said guide tube including an enlarged funnel-like entry portion to facilitate the entry of said flexible cable into said guide tube, the length of said flexible cable being such that when the handle and blade are in the engaged orientation, the flexible cable is disposed in a pre-determined position in the blade;
a camera;
a light-transmitting fiber optic in said flexible cable;
image-transmitting means in said flexible cable; and
a second cable extending from said handle; and
said second cable being adapted to be connected to a video receiver which is receptive to signals from said camera.
8. A laryngoscope according to claim 7 in which said camera is disposed in a chamber in said standard handle, and said image-transmitting flexible cable comprises a coherent fiber optic bundle having one end exposed to a field of view and a second end exposed to said camera.
9. A laryngoscope according to claim 7 in which said light transmitting fiber optic in said flexible cable is adapted to receive light from a separate light source to be transmitted to the field of view.
10. A laryngoscope according to claim 9 in which said camera is disposed in a chamber in said standard handle, and said flexible cable includes a coherent fiber optic bundle having one end exposed to a field of view and a second end exposed to said camera.
11. A laryngoscope according to claim 7 in which said camera is mounted to the distal end of the flexible cable, and the image-transmitting means is a lead from said camera extending through said handle for conveying image data through said handle to a video receiver.
12. A laryngoscope according to claim 7 in which said light source is a battery and bulb combination in said handle, to provide light to said light-transmitting means.
13. A system according to claim 1 in which said guide tube has a closed distal end with an optically clear and transparent window at said distal end, and a wall extending to said entry portion, said wall and said window being imperforate and sterilizeable, whereby a non-sterile and flexible cable can be inserted into said guide tube, and the guide tube disposed of after use without need to sterilize the flexible cable before its next use.
14. A laryngoscope according to claim 7 in which said guide tube has a closed distal end with an optically clear and transparent window at said distal end, and a wall extending to said entry portion, said wall and said window being imperforate and sterilizeable, whereby a non-sterile said flexible cable can be inserted into said guide tube, and the guide tube disposed of after use without need to sterilize the flexible cable before its next use.
15. A system according to claim 1 in which a device to remove the geometric pattern from the signal to the video screen receives and processes the image signal form the camera.
16. A laryngoscope system according to claim 1 in which a conduit mounted to the blade with an exit opening near the distal end of the flexible cable is adapted to discharge a stream of oxygen adjacent to the distal end of the guide tube.
17. A laryngoscope according to claim 7 in which a conduit mounted to the blade with an exit opening near the distal end of the flexible cable is adapted to discharge a stream of oxygen adjacent to the distal end of the guide tube.