All The Claims

1. A library of serum albumin scaffold-based candidate binding proteins, said candidate binding proteins having sequences derived from the human serum albumin (HSA) protein sequence of FIG. 1 (SEQ ID NO: 1), wherein each of said candidate binding proteins has one or more mutated amino acids within at least one of the serum albumin domains or sub-domains.
2. The library of claim 1, wherein at least one candidate binding protein has a mutation in sub-domain IB of HSA.
3. The library of claim 1, wherein at least one candidate binding protein has a mutation in sub-domain IIB of HSA.
4. The library of claim 1, wherein at least one candidate binding protein has a mutation in sub-domain IIIB of HSA.
5. The library of claim 1, wherein at least one candidate binding protein has a mutation in sub-domain IA of HSA.
6. The library of claim 1, wherein at least one candidate binding protein has a mutation in sub-domain IIA of HSA.
7. The library of claim 1, wherein at least one candidate binding protein has a mutation in sub-domain IIIA of HSA.
8. The library of claim 1, wherein at least one candidate binding protein has a mutation in domain I of HSA.
9. The library of claim 1, wherein at least one candidate binding protein has a mutation in domain II of HSA.
10. The library of claim 1, wherein at least one candidate binding protein has a mutation in domain III of HSA.
11. The library of claim 1, wherein at least one candidate binding protein has a mutation in full-length HSA.
12. The library of claim 1, wherein at least one candidate binding protein has mutations in more than one HSA sub-domains.
13. The library of claim 1, wherein at least one candidate binding protein has mutations in two or more amino acids.
14. The library of claim 13, wherein at least one candidate binding protein has mutations in six or more amino acids.
15. The library of claim 14, wherein said at least one candidate binding protein has mutations in no more than 50% of the total amino acids.
16. The library of claim 1, wherein at least one candidate binding protein has a mutated HSA sub-domain fused to the remaining wild-type HSA protein.
17. The library of claim 1, wherein at least one candidate binding protein has a mutated HSA sub-domain fused to at least one additional HSA sub-domain.
18. The library of claim 1, wherein at least one candidate binding protein has a binding affinity for a compound that is at least ten-fold higher than the binding affinity of wild-type HSA for said compound.
19. The library of claim 18, wherein said compound is tumor necrosis factor \u03b1 (TNF-\u03b1).
20. The library of claim 1, wherein at least one candidate binding protein is part of a fusion protein.
21. The library of claim 20, wherein said fusion protein further comprises a complement protein.
22. The library of claim 20, wherein said fusion protein further comprises a toxin protein.
23. The library of claim 1, wherein the candidate binding proteins of said library are immobilized on a solid support.
24. A library of serum albumin scaffold-based proteins, wherein each of said candidate binding proteins has a sequence derived from the human serum albumin (HSA) protein sequence of FIG. 1 (SEQ ID NO: 1) by randomizing a sub-domain or a domain of HSA.
25. The library of claim 24, wherein at least one candidate binding protein comprises more than one randomized HSA sub-domains.
26. A library of nucleic acid-protein fusion molecules, the proteins of said molecules being derived from the human serum albumin sequence of FIG. 1 (SEQ ID NO: 1), wherein each of the proteins has one or more mutated amino acids within at least one of the serum albumin domains or sub-domains, the proteins being attached to the nucleic acids of the fusion molecules by means of a covalent bond.
27. The library claim 26, wherein said nucleic acid comprises DNA.
28. The library of claim 26, wherein said nucleic acid comprises RNA.
29. The library of claim 28, wherein said RNA is an mRNA.
30. The library of claim 26, wherein said nucleic acid of the fusion molecule encodes the protein to which it is bound.
31. The library of claim 26, wherein the nucleic acid-protein fusion molecules of said library are immobilized on a solid support.
32. A library of nucleic acid-protein fusion molecules, wherein the proteins of said molecules are derived from the human serum albumin (HSA) sequence of FIG. 1 (SEQ ID NO: 1) by randomizing a sub-domain or a domain of HSA, and wherein the proteins are attached to the nucleic acids of the fusion molecules by means of a covalent bond.
33. The library of claim 32, wherein the protein of at least one of said nucleic acid-protein fusion molecules comprises more than one randomized HSA sub-domains.
34. A method for obtaining a protein which binds to a compound, said method comprising:
(a) contacting a compound with a library of serum albumin scaffold-based candidate binding proteins under conditions that allow binding to form a compound-protein complex, said proteins being derived from the human wild-type serum albumin protein sequence of FIG. 1 (SEQ ID NO: 1), wherein each of said candidate binding proteins has one or more mutated amino acids within at least one of the serum albumin domains or sub-domains; and
(b) obtaining, from said complex, a protein that binds to said compound.
35. The method of claim 34, said method further comprising mutating at least one sub-domain of the protein obtained in step (b) and repeating steps (a) and (b) using the further mutated protein.
36. The method of claim 34, wherein said compound is a protein.
37. The method of claim 36, wherein said protein is TNF-\u03b1.
38. The method of claim 34, wherein each of said candidate binding proteins is covalently bound to a nucleic acid.
39. The method of claim 38, wherein said nucleic acid is DNA.
40. The method of claim 38, wherein said nucleic acid is RNA.
41. The method of claim 38, wherein said nucleic acid encodes the protein to which it is bound.
42. The method of claim 34, wherein the candidate binding proteins of said library are immobilized on a solid support.
43. A method for obtaining a protein which binds to a compound, said method comprising:
(a) contacting a compound with a library of serum albumin scaffold-based candidate binding proteins under conditions that allow binding to form a compound-protein complex, said candidate binding proteins being derived from the human wild-type serum albumin protein sequence of FIG. 1 (SEQ ID NO: 1) by randomizing a sub-domain or domain of HSA; and
(b) obtaining, from said complex, a protein that binds to said compound.
44. The method of claim 43, wherein at least one of the candidate binding proteins of said library comprises more than one randomized HSA sub-domains.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

What is claimed is:

1. A centralized power distribution unit for a vehicular thin brushless motor, wherein said centralized power distribution unit is formed into a ring configuration and can concentratedly distribute current to stator windings of the motor, comprising:
a plurality of bus bars each having a terminal portion to be connected to a power source and one or more tabs to be connected respectively to one or more of the stator windings, the bus bars provided correspondingly with phases of the motor; and
a resin insulating layer that covers the bus bars;
wherein
each of the bus bars comprises a metal strip that is bent, in a thickness direction of the metal strip, into a substantially annular shape, and diameters of the annular shapes of the bus bars are set to be different from one another depending on the corresponding motor phase; and
the bus bars are stacked in a radial direction of the centralized power distribution unit, mutually separated by a predetermined gap.
2. The centralized power distribution unit according to claim 1, wherein at least one of the terminal portions includes a first section extending in a first direction that is a substantially radial direction of the centralized power distribution unit, a second section extending in a second direction substantially perpendicular to the first direction, and a ramp section connecting the first and second sections and extending in a third direction that is different from the first and second directions.
3. The centralized power distribution unit according to claim 1, wherein at least one slit is provided in at least one of the terminal portions.
4. The centralized power distribution unit according to claim 3, wherein a section of the at least one of the terminal portions is covered by a sealing material, and the at least one slit is provided in the section covered by the sealing material.
5. The centralized power distribution unit according to claim 3, wherein the at least one slit extends in a longitudinal direction of the terminal portion.
6. A method of producing a centralized power distribution unit for a vehicular thin brushless motor wherein said centralized power distribution unit is formed into a ring configuration and can concentratedly distribute current to stator windings of the motor, the method comprising:
stamping out a conductive metal plate into a plurality of strips, the strips stamped out simultaneously by press molding;
bending the strips in a thickness direction into a substantially annular shape to obtain a plurality of bus bars in the form of rings, the rings being mutually different in diameter, the bus bars each having a terminal portion to be connected to a power source and one or more tabs to be connected respectively to one or more windings of a stator;
providing the bus bars correspondingly with phases of the motor;
stacking the strips in a radial direction of the centralized power distribution unit, mutually separated by a predetermined gap; and
covering the bus bars with a resin insulating layer.
7. A method of producing bus bars, comprising:
stamping out a conductive metal plate into a plurality of strips, the strips stamped out simultaneously by press molding; and
bending the strips in a thickness direction to obtain a plurality of rings, the rings being mutually different in diameter.
8. The method of producing bus bars according to claim 7, wherein, in the step of stamping out, at least one of (a) a terminal portion and (b) one or more tabs are integrally stamped with a main body of the bus bar.
9. The method of producing bus bars according to claim 8, wherein, in the step of stamping out, the bus bars are stamped out in a state where the main bodies are laid out in parallel, and in a state where both ends of the main bodies are substantially aligned with one another.
10. The method of producing bus bars according to claim 7, wherein, in the step of stamping out, a terminal portion and one or more tabs are integrally stamped with a main body of the bus bar.
11. The method of producing bus bars according to claim 10, wherein, in the step of stamping out, the terminal portion and the one or more tabs of a predetermined one of the bus bars are laid out to be directed to a center of a row of the bus bars, the predetermined one of the bus bars being positioned at an outermost side among the bus bars which are placed in parallel.
12. A method of producing a centralized power distribution unit wherein said centralized power distribution unit is formed into a ring configuration and can concentratedly distribute current to stator windings of a motor, the method comprising:
stamping out a conductive metal plate into a plurality of strips, the strips stamped out simultaneously by press molding;
bending the strips in a thickness direction to obtain a plurality of rings, the rings being mutually different in diameter; and
stacking the strips in a radial direction of the centralized power distribution unit mutually, separated by a predetermined gap.
13. The method according to claim 12, wherein each bus bar includes a terminal portion that connects to a power source, further comprising bending the terminal portion into a bent configuration.
14. The method according to claim 13, wherein bending at least one of the terminal portions comprises bending the terminal portion such that the at least one of the terminal portions includes a first section extending in a first direction that is a substantially radial direction of the centralized power distribution unit, a second section extending in a second direction substantially perpendicular to the first direction, and a ramp section connecting the first and second sections and extending in a third direction that is different from the first and second directions.
15. The method according to claim 13, further comprising providing at least one slit in at least one of the terminal portions.
16. The method according to claim 15, further comprising a section of the at least one of the terminal portions by a sealing material, wherein the at least one slit is provided in the section covered by the sealing material.
17. The method according to claim 15, wherein the at least one slit extends in a longitudinal direction of the terminal portion.

1. A method for creating a player made tournament, the method comprising:
displaying a tournament creation page, the tournament creation page including
a plurality of variables, and
at least one option for each of the plurality of variables;

receiving the at least one option for each of the plurality of variables, operational parameters of the player made tournament substantially comprising the at least one option for each of the plurality of variables received;
processing the operational parameters to create a player made tournament; and
displaying the player made tournament on a tournament main page.
2. The method of claim 1, wherein the plurality of variables comprises a tournament name, a tournament fee, and a bankroll.
3. The method of claim 2, wherein the plurality of variables further comprises a tournament description, a tournament type, a number of players paid out, a tracks and dates selection, a plurality of bet types, a minimum number of wagers, a maximum number of wagers, a minimum amount per wager, a maximum amount per wager, a minimum number of wagers per race, and a maximum number of wagers per race.
4. The method of claim 3, wherein the at least one option for the tournament type comprises two options, the two options comprise bankroll and return on investment.
5. The method of claim 1, wherein the at least one option for at least some of the plurality of variables comprises at least two options, the at least two options comprise at least one pre-determined option and at least one custom option.
6. The method of claim 1, wherein the player made tournament comprises real-life horse racing events.
7. The method of claim 1, wherein the player made tournament comprises real-life dog racing events.
8. The method of claim 1, and further comprising receiving requests to join the player made tournament displayed on the tournament main page.
9. The method of claim 1, wherein the player made tournament comprises one of a guaranteed prize pool and a non-guaranteed prize pool.
10. A method for operating a player made tournament for real-life sporting events, the method comprising:
receiving a request to create a player made tournament for real-life sporting events;
displaying a tournament creation page, the tournament creation page including a plurality of variables and at least one field corresponding to each of the plurality of variables, the at least one field configured to receive an option for the corresponding variable;
receiving options for each of the plurality of variables, operational parameters for the player made tournament for real sporting events substantially comprising the options for each of the plurality of variables received;
processing the operational parameters to create a player made tournament for real-life sporting events;
displaying the player made tournament for real-life sporting events on a tournament main page, the tournament main page including a display of at least a portion of the operational parameters of the player made tournament for real-life sporting events; and
receiving requests to participate in the player made tournament for real-life sporting events.
11. The method of claim 10, wherein the real-life sporting events comprise horse racing.
12. The method of claim 10, wherein the real-life sporting events comprise dog racing.
13. The method of claim 10, wherein the plurality of variables comprises a tournament name, a tournament fee, a bankroll, a tournament description, a tournament type, a number of players paid out, a tracks and dates selection, a plurality of bet types, a minimum number of wagers, a maximum number of wagers, a minimum amount per wager, a maximum amount per wager, a minimum number of wagers per race, and a maximum number of wagers per race.
14. The method of claim 10, wherein the player made tournament for real-life sporting events comprises one of a guaranteed prize pool and a non-guaranteed prize pool.
15. The method of claim 10, and further comprising displaying a tournament detail page for the player made tournament for real-life sporting events in response to a request to view the tournament detail page, the tournament detail page including the operational parameters of the player made tournament for real-life sporting events, a wager history, and a roster of participants.
16. The method of claim 10, and further comprising creating a bankroll account and a pool entry fee account for the player made tournament for real-life sporting events.
17. The method of claim 16, and further comprising initiating a funds transfer from a player member account to the bankroll account and the pool entry fee account for those who request to participate in the player made tournament for real-life sporting events.
18. A system for creating a player made tournament, the system comprising:
at least one computer server having a processing unit and a database, the at least one computer server configured to:
receive and process a request to create a player made tournament;
display a tournament creation page in response to the request to create a player made tournament, the tournament creation page including a plurality of variables and at least one option for each of the plurality of variables;
receive the at least one option for each of the plurality of variables, operational parameters of the player made tournament comprising the at least one option for each of the plurality of variables received;
process the operational parameters using the processing unit to create a player made tournament;
store the operation parameters in the database of the computer server; and
display the player made tournament on a tournament main page.
19. The system of claim 18, wherein the player made tournament comprises real-life sporting events.
20. The system of claim 19, wherein the plurality of variables comprise a tournament name, a tournament fee, a bankroll, a tournament description, a tournament type, a number of players paid out, a tracks and dates selection, a plurality of bet types, a minimum number of wagers, a maximum number of wagers, a minimum amount per wager, a maximum amount per wager, a minimum number of wagers per race, and a maximum number of wagers per race.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A system for testing light-emitting diodes (LEDs) on a motherboard, comprising:
an insulating plate positioned on the motherboard and configured with optical fibers for inducing beams sourced from the LEDs and transmitting the beams;
a circuit board receiving the beams transmitted from the LED’s, the circuit board being connected to the insulating plate with the optical fibers, the circuit board comprising at least one photoresistor configured for sensing the beams sourced from the LEDs to obtain influence values and for transmitting the influence values; and
a computer receiving the influence values from the circuit board, the computer being configured for controlling the LEDs to power on or power off by controlling luminous intensities of the LEDs, the computer configured for detecting whether the influence values are within a photosensitive range when the LEDs are powered on, the computer configured for detecting whether resistance values of all the at least one photoresistor are equal to a dark resistance when the LEDs are powered off, and the computer configured for reporting test results.
2. The system for testing LEDs on a motherboard as described in claim 1, wherein the circuit board further comprises:
an AD converter configured for converting the analog signals into influence values;
a level changer configured for adjusting power levels of the influence values to be compatible as an input of a processor; and
the processor configured for processing the influence values to obtain the processed influence values, and the processor configured for transmitting the processed influence values to the level changer for changing electric properties between the processor and a serial port.
3. The system for testing LEDs on a motherboard as described in claim 1, wherein the circuit board further comprising an LED lamp configured for emitting different color lights to indicate the test results.
4. The system for testing LEDs on a motherboard as described in claim 1, wherein the computer comprises:
a controlling module configured for controlling the luminous intensities of the LEDs, for controlling the given number of at least one photoresistor to sense the beams sourced from the LEDs and obtain the influence values, and for controlling the circuit board to process the influence values;
a detecting module configured for determining whether all the LEDs pass or fail the test by detecting whether the influence values are within the photosensitive range when the LEDs are powered on, by detecting whether resistance values of all the at least one photoresistor are equal to the dark resistance when the LEDs are powered off and by comparing the number of photosensitive photoresistors with the number of the LEDs and for reporting the test results;
a counting module configured for counting the number of the photosensitive photoresistors whose influence values are within the photosensitive range when the LEDs are powered on;
a result feedback module configured for transmitting the test results to the circuit board; and
an error ascertaining module configured for ascertaining whether each of the LEDs is in a workable state or in an unworkable state according to the test results.
5. The system for testing LEDs on a motherboard as described in claim 1, wherein the insulating plate comprises multi-holes corresponding to a plurality of components on the motherboard and covers on the motherboard via the multi-holes.
6. The system for testing LEDs on a motherboard as described in claim 1, wherein the insulating plate comprises optical fibers within a pipeline for each of the LEDs and each of the LEDs connected to each of the given number of at least one photoresistor via the optical fibers respectively.
7. The system for testing LEDs on a motherboard as described in claim 6, wherein the number of the given number of at least one photoresistor is equal to the number of the LEDs on the motherboard.
8. A method for testing light-emitting diodes (LEDs) on a motherboard, the method comprising:
covering the motherboard with a insulating plate and connecting the LEDs of the motherboard to a circuit board via optical fibers of the insulating plate, wherein the circuit board comprises at least one photoresistor;
obtaining an influence value of each of the LEDs;
detecting whether resistance values of the given number of at least one photoresistor are equal to corresponding dark resistances when each of the LEDs are powered off;
detecting whether the influence value of each of the LEDs is within a photosensitive range of the at least one photoresistor when each of the LEDs is powered on; and
reporting test results denoting that each of the LEDs passes the test, if the resistance values of all of the given number of at least one photoresistor are equal to corresponding dark resistances and the influence value of each of the LEDs is within the photosensitive range; or
reporting test results denoting that each of the LEDs fails the test, if the resistance values of all of the at least one photoresistor are not equal to corresponding dark resistances or the influence value of each of the LEDs is not in the photosensitive range.
9. The method for testing LEDs on a motherboard as described in claim 8, further comprising steps of:
setting the LEDs to power off by controlling luminous intensities of the LEDs;
sensing beams sourced from the LEDs to obtain analog signals via the given number of at least one photoresistor;
converting the analog signals to influence values;
processing the influence values by a processor;
changing electric properties between the processor and a serial port of the circuit board;
transmitting the influence values to a computer via the serial port; and
calculating resistance values of the given number of at least one photoresistor.
10. The method for testing LEDs on a motherboard as described in claim 8, further comprising steps of:
setting the LEDs to power on by controlling luminous intensities of the LEDs;
sensing beams sources from the LEDs to obtain analog signals via the given number of at least one photoresistor;
converting the analog signals to influence values;
processing the influence values by a processor;
changing electric properties between the processor and a serial port of the circuit board;
transmitting the influence values to a computer via the serial port;
counting the number of photosensitive photoresistors whose influence values are in a photosensitive range of the given number of at least one photoresistor when each of the LEDs is powered on; and
determining whether the influence value of all of the LEDs is within a photosensitive range by comparing the number of the photosensitive photoresistors with the number of the LEDs.
11. The method for testing LEDs on a motherboard as described in claim 8, further comprising steps of:
transmitting the test results to the circuit board; and
indicating the test results with different color lights in an LED lamp of the circuit board.
12. The method for testing LEDs on a motherboard as described in claim 8, further comprising a step of:
ascertaining each of the LEDs on the motherboard is in a workable state or in an unworkable state according to the test results.