All The Claims

1. A composition for electrophoresis of nucleic acids, the buffer comprising:
(i) TAPS, TAPSO, or Asparagine;
(ii) a compound having a structure of Formula I, wherein:
HO(CH2)m3C\u2014N(CH2)n-OH2\u2003\u2003Formula I
wherein m is an integer of from 1 to 3, and n is an integer of form 1 to 4; and
(iii) a metal-chelating agent.
2. The composition of claim 1, wherein said metal-chelating agent is EDTA.
3. The composition of claim 1, wherein the composition is substantially detergent-free.
4. The composition of claim 1, having a pH greater than 7.
5. The composition of claim 1 comprising TAPS and the compound of Formula I.
6. The composition of claim 5, wherein said metal-chelating agent includes EDTA.
7. The composition of claim 5, wherein the composition is substantially detergent-free.
8. The composition of claim 5, wherein the compound of Formula I is present at a concentration of 50-200 mM, wherein TAPS is present at a same concentration as the compound of Formula I, and wherein the EDTA is present at 1-2 mM.
9. The composition of claim 5, wherein the compound of Formula I is present at a concentration of 50 mM and the EDTA is present at a concentration of 2 mM.
10. The composition of claim 5, having a pH no less than 7.
11. The composition of claim 1, further comprising one or more organic polymers.
12. The composition of claim 11, wherein the one or more organic polymers comprise a sieving component comprising a non-crosslinked acrylamide polymer, and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide, N-substituted polyacrylamide, N-monosubstituted polyacrylamides, polymethacrylamide, polyvinylpyrrolidone, and poly(N,Ndimethylacrylamide).
13. The composition of claim 11, wherein the one or more organic polymers comprise a sieving component comprising one or more polymers selected from the group consisting of linear polyacrylamide, branched acrylamide polymers, and star-shaped acrylamide polymers.
14. An apparatus for resolving samples, comprising:
an electrophoretic channel extending and communicating between anodic and cathodic buffer chambers; and
a buffer, wherein said buffer comprises:
a) a compound having a structure of Formula I, wherein:
HO(CH2)m3C\u2014N(CH2)n\u2014OH2\u2003\u2003Formula I

m is an integer of from 1 to 3, and n is an integer of form 1 to 4; Bis Tris,

b) one or both of TAPS and TAPSO; and
c) a metal-chelating agent.
15. The apparatus of claim 14, wherein the channel is defined by a capillary tube.
16. The apparatus of claim 14, wherein the compound of Formula I is contained in said anodic and cathodic buffer chambers and in said channel.
17. The apparatus of claim 14, further comprising a sieving medium held in said channel.
18. (canceled)
19. A method of electrophoresis of nucleic acids, comprising:
(a) adding a buffer into an electrophoretic channel, wherein said buffer comprises:
i) TAPS;
ii) a metal-chelating agent; and
iii) a compound having a structure of Formula I, wherein:
HO(CH2)m3C-N(CH2)n\u2014OH2\u2003\u2003Formula I
m is an integer of from 1 to 3, and n is an integer of form 1 to 4;

(b) adding a sample including nucleic acids to be analyzed into said electrophoretic channel; and
(c) applying an electromotive potential across the electrophoretic channel.
20. The method of claim 19, wherein the channel is defined by a capillary tube.
21. The method of claim 20, wherein during the application of the electromotive potential, the buffer has a pH of no less than 7.5.
22.-42. (canceled)
43. The composition of claim 5, wherein the compound of Formula I has the structure wherein m is 1 and n is 2.
44. The method of claim 19, wherein the electrophoretic channel contains a sieving medium comprising one or organic polymers.
45. The method of claim 44, wherein the one or more organic polymers comprise a non-crosslinked acrylamide polymer, and a surface interaction component comprising one or more non-crosslinked polymers selected from the group consisting of N,N-disubstituted polyacrylamide, N-substituted polyacrylamide, N-monosubstituted polyacrylamides, polymethacrylamide, polyvinylpyrrolidone, and poly(N,Ndimethylacrylamide).
46. The method of claim 44, wherein the one or more organic polymers comprise one or more polymers selected from the group consisting of linear polyacrylamide, branched acrylamide polymers, and star-shaped acrylamide polymers.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A voice coil motor assembly comprising:
a housing comprising a top surface;
a voice coil motor received in the housing;
a cover secured to the voice coil motor and resisting the top surface of the housing, wherein the cover comprises a top surface defining an aperture, a bottom surface opposite to the top surface of the cover, a limiting element protruding from the top surface of the cover, and a frame formed around the brim of the bottom surface of the cover; the frame comprises a top surface and a bottom surface, the top surface of the frame resists the top surface of the housing, and the bottom surface of the frame resists the voice coil motor; and
a transparent board adhering to the top surface of the cover and covering the aperture, the limiting element engaging with the transparent board and configured for restraining movement of the transparent board.
2. The assembly as described in claim 1, wherein the distance between the bottom surface of the frame and the bottom surface of the cover is equal to or greater than the greatest movement distance of the voice coil motor.
3. The assembly as described in claim 1, wherein the voice coil motor comprises a bracket, a barrel, and an elastic element, the barrel is moveably received in the bracket, and the elastic element is secured to a top of the barrel.
4. The assembly as described in claim 3, wherein four positioning posts protrude from a top of the bracket, and the frame of the cover defines four postholes, the positioning posts are engaged in the postholes to secure the cover to the bracket.
5. The assembly as described in claim 3, wherein four fixing posts protrude from a top of the barrel, and the elastic element defines four fixing holes, the fixing posts are engaged in the fixing holes to secure the elastic element to the top of the barrel.
6. The assembly as described in claim 5, wherein the bottom surface of the cover defines two first recessed portions and two second recessed portions, each of the first recessed portions spatially corresponds to one of two opposite fixing holes, and each of the second recessed portions spatially corresponds to one of the other fixing holes.
7. The assembly as described in claim 5, wherein each of the first recessed portions extends through the cover.
8. The assembly as described in claim 3, wherein a pair of latching protrusions protrude from each of opposite sidewalls of the bracket, and each of the opposite sidewalls of the housing defines a pair of through holes, each of the latching protrusions extends through each of the through holes and is latchingly engaged with the through hole to secure the voice coil motor to the housing.
9. The assembly as described in claim 1, wherein the top surface of the housing defines a receiving space receiving the voice coil motor, and the limiting element is exposed to the housing via the receiving space.
10. The assembly as described in claim 1, wherein the shape of the transparent board conforms to the limiting element.

1. A circuit, comprising:
a control circuit; and
a voltage sensor coupled to the control circuit;
wherein the control circuit, responsive to the voltage sensor, is configured to:
detect a first classification voltage within a classification voltage range defined by a lower classification voltage limit and upper classification voltage limit;
detect, after detecting the first classification voltage, an indexing voltage outside of the classification voltage range; and
detect, after detecting the indexing voltage, a second classification voltage within the classification voltage range, wherein the control circuit is further configured to set an indicator signal to a first predetermined state indicating a power type based on the detected first classification voltage, indexing voltage and second classification voltage.
2. The circuit of claim 1, wherein the first predetermined state indicates a high power type.
3. The circuit of claim 1, wherein after the control circuit detects the second classification voltage, the control circuit is further configured to:
detect a signature voltage below the classification voltage range and set the indicator signal to the first predetermined state in response to the detected signature voltage.
4. The circuit of claim 3, wherein in the event that the control circuit does not detect the signature voltage, the control circuit is further configured to:
set the indicator signal to a second predetermined state indicating a low power type.
5. The circuit of claim 1, wherein in the event that the control circuit detects the first classification voltage and does not detect the indexing voltage, the control circuit is further configured to:
set the indicator signal to a second predetermined state indicating a low power type.
6. The circuit of claim 1, further comprising:
at least one current source coupled to the control circuit, wherein the control circuit is configured to control the at least one current source to provide a first predetermined classification current responsive to the detected first classification voltage and a second predetermined classification current responsive to the detected second classification voltage.
7. The circuit of claim 1, further comprising:
at least one current source responsive to the control circuitry, wherein the control circuitry is configured to control the at least one current source to:
provide a first predetermined classification current responsive to the detected first classification voltage, wherein the first predetermined classification current exceeds a default classification value limit;
provide a draw down current responsive to the detected indexing voltage; and
provide a second predetermined classification current exceeding a default classification value limit responsive to the detected second classification voltage.
8. The circuit of claim 7, wherein the draw down current is of a value greater than the default classification value limit.
9. A circuit, comprising:
a control circuit;
a voltage sensor coupled to the control circuit; and
a current source coupled to the control circuit;
wherein the control circuit, responsive to the voltage sensor, is configured to:
detect a first classification voltage within a classification voltage range defined by a lower classification voltage limit and upper classification voltage limit;
generate a first classification current, by the current source, in response to detecting the first classification voltage;
determine if an indexing voltage is detected, wherein the indexing voltage is outside of the classification voltage range;
if the indexing voltage is not detected, then set an indicator signal to a low power state based on an operating voltage; and
if the indexing voltage is detected, then:
detect a second classification voltage within the classification voltage range, and
generate a second classification current, by the current source, in response to detecting the second classification voltage.
10. The circuit of claim 9, wherein after the control circuit is configured to generate the second classification current by the current source, the control circuit is further configured to:
change the second classification current, by the current source, to a different current than a default classification limit.
11. The circuit of claim 10, wherein after the control circuit is configured to change the second classification current by the current source, the control circuit is further configured to:
detect a signature voltage, wherein:
if the signature voltage is not detected, then set an indicator signal to a low power state based on an operating voltage; and
if the signature voltage is detected, then wait for an operating voltage to be detected.
12. The circuit of claim 11, wherein the control circuit is further configured to set the indicator signal to the low power state based on the operating voltage comprises the control circuit is configured to:
wait for the voltage sensor to detect the operating voltage; and
set the indicator signal to the state indicating the low power state.
13. The circuit of claim 12, wherein the control circuit is configured to closing a switch to a load after one of the control circuit is configured to detect the operating voltage and the control circuit is configured to set the indicator signal to the low power state based on the operating voltage.
14. The circuit of claim 12, further comprising:
at least one current source coupled to the control circuit, wherein the control circuit is configured to control the at least one current source to provide a first predetermined classification current responsive to the detected first classification voltage and a second predetermined classification current responsive to the detected second classification voltage.
15. An Ethernet device, comprising:
a control circuit; and
a voltage sensor coupled to the control circuit;
wherein the control circuit, responsive to the voltage sensor, is configured to:
detect a first classification voltage within a classification voltage range defined by a lower classification voltage limit and upper classification voltage limit;
detect, after detecting the first classification voltage, an indexing voltage outside of the classification voltage range; and
detect, after detecting the indexing voltage, a second classification voltage within the classification voltage range, wherein the control circuit is further configured to set an indicator signal to a first predetermined state indicating a power type based on the detected first classification voltage, indexing voltage and second classification voltage.
16. The Ethernet device of claim 15, wherein the first predetermined state indicates a high power type.
17. The Ethernet device of claim 15, wherein after the control circuit detects the second classification voltage, the control circuit is further configured to:
detect a signature voltage below the classification voltage range and set the indicator signal to the first predetermined state in response to the detected signature voltage.
18. The Ethernet device of claim 17, wherein in the event that the control circuit does not detect the signature voltage, the control circuit is further configured to:
set the indicator signal to a second predetermined state indicating a low power type.
19. The Ethernet device of claim 15, wherein in the event that the control circuit detects the first classification voltage and does not detect the indexing voltage, the control circuit is further configured to:
set the indicator signal to a second predetermined state indicating a low power type.
20. The Ethernet device of claim 15, further comprising:
at least one current source coupled to the control circuit, wherein the control circuit is configured to control the at least one current source to provide a first predetermined classification current responsive to the detected first classification voltage and a second predetermined classification current responsive to the detected second classification voltage.
21. The Ethernet device of claim 15, further comprising:
at least one current source responsive to the control circuitry, wherein the control circuitry is configured to control the at least one current source to:
provide a first predetermined classification current responsive to the detected first classification voltage, wherein the first predetermined classification current exceeds a default classification value limit;
provide a draw down current responsive to the detected indexing voltage; and
provide a second predetermined classification current exceeding a default classification value limit responsive to the detected second classification voltage.
22. The Ethernet device of claim 21, wherein the draw down current is of a value greater than the default classification value limit.
23. An Ethernet device, comprising:
a control circuit;
a voltage sensor coupled to the control circuit; and
a current source coupled to the control circuit;
wherein the control circuit, responsive to the voltage sensor, is configured to:
detect a first classification voltage within a classification voltage range defined by a lower classification voltage limit and upper classification voltage limit;
generate a first classification current, by the current source, in response to detecting the first classification voltage;
determine if an indexing voltage is detected, wherein the indexing voltage is outside of the classification voltage range;
if the indexing voltage is not detected, then set an indicator signal to a low power state based on an operating voltage; and
if the indexing voltage is detected, then:
detect a second classification voltage within the classification voltage range, and
generate a second classification current, by the current source, in response to detecting the second classification voltage.
24. The Ethernet device of claim 23, wherein after the control circuit is configured to generate the second classification current by the current source, the control circuit is further configured to:
change the second classification current, by the current source, to a different current than a default classification limit.
25. The Ethernet device of claim 24, wherein after the control circuit is configured to change the second classification current by the current source, the control circuit is further configured to:
detect a signature voltage, wherein:
if the signature voltage is not detected, then set an indicator signal to a low power state based on an operating voltage; and
if the signature voltage is detected, then wait for an operating voltage to be detected.
26. The Ethernet device of claim 25, wherein the control circuit is further configured to set the indicator signal to the low power state based on the operating voltage comprises the control circuit is configured to:
wait for the voltage sensor to detect the operating voltage; and
set the indicator signal to the state indicating the low power state.
27. The Ethernet device of claim 26, wherein the control circuit is configured to closing a switch to a load after one of the control circuit is configured to detect the operating voltage and the control circuit is configured to set the indicator signal to the low power state based on the operating voltage.
28. The Ethernet device of claim 27, further comprising:
at least one current source coupled to the control circuit, wherein the control circuit is configured to control the at least one current source to provide a first predetermined classification current responsive to the detected first classification voltage and a second predetermined classification current responsive to the detected second classification voltage.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

I claim:

1. An air applicator for placing a series of parts onto a substrate, the air applicator comprising:
a passage having an upstream end and a downstream end opposite the upstream end; and
one or more orifices adapted to direct an air flow towards the downstream end;
wherein the air applicator increases the spacing between successive parts from a first spacing at the upstream end to a second spacing at the downstream end, and increases the velocities of the parts from a first velocity at the upstream end to a second velocity at the downstream end.
2. The air applicator of claim 1, further comprising a cutting device disposed proximal to the upstream end; said cutting device being adapted to sever parts from at least one material supply.
3. The air applicator of claim 2, wherein the cutting device is a die cutter.
4. The air applicator of claim 1, further comprising a moving substrate onto which the parts are applied, the moving substrate being disposed proximal to the downstream end.
5. The air applicator of claim 4, wherein the moving substrate is a material web, a supply of discrete objects, or a conveying device.
6. The air applicator of claim 4, wherein the second velocity is approximately equal to the velocity of the moving substrate.
7. The air applicator of claim 1, wherein the one or more orifices may be manipulated to adjust the air flow properties.
8. The air applicator of claim 1, further comprising a regulator adapted to control properties of the air flow.
9. The air applicator of claim 1, wherein the parts are foam panels, ribbons, sheets, yarns or strands.
10. The air applicator of claim 1, wherein the upstream end comprises a splayed opening.
11. The air applicator of claim 1, further comprising:
a pressure regulator adapted to control the pressure of air entering the one or more orifices; and
a control system adapted to control the pressure regulator to thereby control the second spacing and second velocity.
12. The air applicator of claim 11, wherein the control system is further adapted to detect a value of at least one of the second speed and second spacing and to control at least one of the second spacing and second velocity based on the detected value.
13. An internal air applicator for placing a series of parts onto a substrate, the internal air applicator comprising:
a substantially enclosed passage having an upstream end and a downstream end opposite the upstream end; and
one or more orifices adapted to direct an air flow towards the downstream end;
wherein the internal air applicator increases the spacing between successive parts from a first spacing at the upstream end to a second spacing at the downstream end, and increases the velocities of the parts from a first velocity at the upstream end to a second velocity at the downstream end.
14. The internal air applicator of claim 13, wherein the substantially enclosed passage has a rounded profile.
15. The internal air applicator of claim 13, wherein the substantially enclosed passage has a rectilinear profile.
16. The internal air applicator of claim 13, further comprising a moving substrate onto which parts are applied, the moving substrate being disposed proximal to the downstream end.
17. The internal air applicator of claim 16, wherein the moving substrate comprises absorbent garment subassemblies and the parts comprise yarns.
18. The internal air applicator of claim 13, further comprising a regulator adapted to control properties of the air flow.
19. The internal air applicator of claim 13, wherein the one or more orifices comprise an eductor.
20. The internal air applicator of claim 13, wherein the substantially enclosed passage is a coanda passage.
21. An absorbent garment comprising:
a topsheet;
a backsheet;
an absorbent core disposed between the topsheet and backsheet; and
one or more yarns assembled to the garment using the internal air applicator of claim 13.
22. An external air applicator for placing a series of parts onto a substrate, the air applicator comprising:
a guide plate having an upstream end and a downstream end opposite the upstream end, the guide plate defining an open passage; and
one or more orifices adapted to direct an air flow towards the downstream end;
wherein the external air applicator increases the spacing between successive parts from a first spacing at the upstream end to a second spacing at the downstream end, and increases the velocities of the parts from a first velocity at the upstream end to a second velocity at the downstream end.
23. The external air applicator of claim 22, further comprising guide rails disposed on the guide plate on either side of the open passage and extending at least partly between the upstream end and the downstream end.
24. The external air applicator of claim 22, further comprising one or more guide pins disposed on the guide plate on either side of the open passage.
25. The external air applicator of claim 22, further comprising rows of two or more guide pins disposed on the guide plate on each side of the open passage.
26. The external air applicator of claim 22, wherein the one or more orifices comprise angled slots through the guide plate.
27. The external air applicator of claim 22, wherein the one or more orifices comprise an air knife.
28. The external air applicator of claim 27, wherein the air knife is adjustably mounted.
29. The external air applicator of claim 22, further comprising a regulator adapted to control properties of the air flow.
30. The external air applicator of claim 22, further comprising a moving substrate onto which parts are applied, the moving substrate being disposed proximal to the downstream end.
31. The external air applicator of claim 30, wherein the moving substrate comprises absorbent garment subassemblies and the parts comprise foam panels.
32. An absorbent garment comprising:
a topsheet;
a backsheet;
an absorbent core disposed between the topsheet and backsheet; and
one or more supplemental core layers assembled to the garment using the external air applicator of claim 22.
33. An absorbent garment core forming apparatus comprising:
a rotary vacuum drum having a vacuum surface;
a core forming chamber adapted to provide a supply of absorbent core material to the vacuum surface, the core forming chamber being disposed adjacent the rotary vacuum drum;
one or more air applicators disposed adjacent the rotary vacuum drum;
wherein the one or more air applicators are adapted to apply discrete parts to at least one of the vacuum surface andor the absorbent core material.
34. An absorbent garment core forming apparatus comprising:
a rotary vacuum drum having a vacuum surface;
a supply of tissue material, the supply of tissue material being disposed on at least a portion of the vacuum surface of the rotary vacuum drum;
a core forming chamber adapted to provide a supply of absorbent core material to the supply of tissue material, the core forming chamber being disposed adjacent the rotary vacuum drum; and
one or more air applicators disposed adjacent the rotary vacuum drum;
wherein the one or more air applicators are adapted to apply discrete parts to at least one of the supply of tissue material andor the absorbent core material.
35. An absorbent garment core forming apparatus comprising:
a rotary vacuum drum;
a core forming chamber adapted to provide a supply of absorbent core material, the core forming chamber being disposed adjacent the rotary vacuum drum; and
one or more air applicators disposed at least partially within the core forming chamber;
wherein the one or more air applicators are adapted to apply discrete parts into the core forming chamber.
36. A method for placing a series of parts onto a substrate, the method comprising: providing a series of parts to a passage having an upstream end and a downstream end opposite the upstream end;
providing an air flow to the passage, the air flow being directed towards the downstream end;
using the air flow to increase the spacing between the parts from a first spacing at the upstream end to a second spacing at the downstream end;
using the air flow to increases the velocities of the parts from a first velocity at the upstream end to a second velocity at the downstream end; and
depositing the parts, at a second spacing and a second velocity, on a substrate positioned adjacent the downstream end.
37. The method of claim 36, further comprising severing the parts from a continuous material supply as they enter the passage.
38. The method of claim 36, further comprising controlling the second speed and second spacing by modulating a pressure regulator adapted to control the pressure of air entering the one or more orifices.
39. The method of claim 38, further comprising detecting a value of at least one of the second speed and second spacing and controlling at least one of the second spacing and second velocity based on the detected value.
40. A disposable absorbent garment made according to the method of claim 36.