All The Claims

1. A method of designing a probe panel for a flow cytometer, the method comprising:
determining a distortion factor that quantifies spillover effect caused by emission of a first label, intended to be measured in a first channel, into a second channel;
inputting a maximum expected signal of a first probe-label combination including the first label and a first probe;
calculating an increase in detection limit in the second channel based on the distortion factor and the maximum expected signal of the first probe-label combination; and
selecting a probe-label combination to include in the probe panel based on the calculated increase in detection limit.
2. The method of claim 1, wherein the distortion factor is an estimate of an increase in detection limit in the second channel as a function of an emission intensity of the first probe-label combination.
3. (canceled)
4. The method of claim 2, wherein the increase in detection limit in the second channel is caused by an increase in a measurement error as a function of the emission intensity of the first probe-label combination.
5. The method of claim 2, wherein the distortion factor is calculated using a crosstalk index.
6. The method of claim 1 wherein the distortion factor is mathematically modified by a coefficient representing the coexpression pattern of antigens corresponding to the first probe-label combination and a second probe-label combination, the second probe-label combination intended to be measured in the second channel.
7. The method of claim 1 further comprising:
determining a distortion factor for each label in a first potential probe panel to calculate a total increase in detection limit in the second channel.
8. The method of claim 7, wherein selecting the probe-label combination is based on a comparison of the calculated total increase in detection limit with an expected minimum signal in the second channel.
9. The method of claim 7, further comprising calculating a total increase in detection limit for each probe in the first potential probe panel.
10. The method of claim 9, further comprising:
calculating a total increase in detection limit for each probe in a second potential probe panel; and
selecting the probe panel based on a comparison of the calculated total increase in detection limit for each probe in the first potential probe panel with the calculated total increase in detection limit for each probe in the second potential probe panel.
11. The method of claim 9, further comprising:
calculating a total increase in detection limit for each probe in a second potential probe panel; and
selecting the probe panel based on the calculated total increase in detection limit for a prioritized probe in the first potential probe panel and the second potential probe panel.
12. (canceled)
13. A method of designing a probe panel for a flow cytometer, the method comprising:
identifying a first probe and a second probe;
identifying an expected minimum signal of the first probe;
determining a first detection limit of the first probe based on a potential label associated with the second probe;
determining a second detection limit of the first probe based on a different potential label associated with the second probe;
selecting which label to associate with the second probe for the probe panel based on the first detection limit, the second detection limit, and the expected minimum signal of the first probe.
14. The method of claim 13, wherein determining the first detection limit comprises multiplying a distortion factor by a maximum expected signal in a detection channel intended to measure the potential label associated with the second probe.
15. The method of claim 14, wherein determining the first detection limit further comprises multiplying the distortion factor by a coefficient representing an antigenic coexpression pattern.
16. The method of claim 15, wherein the coefficient is either one or zero.
17. The method of claim 14, wherein said maximum expected signal is based in part on an expected antigen density on a target cell.
18. The method of claim 17, wherein said maximum expected signal is further based on the potential label associated with the second probe.
19. (canceled)
20. The method of claim 14, wherein the first probe is intended to be detected in a first channel, and wherein determining the first detection limit of the first probe comprises multiplying a distortion factor by a maximum expected signal for each channel of the flow cytometer other than the first channel.
21. The method of claim 20, wherein determining the first detection limit of the first probe is based on a linear superpositioning model of CV enlargements.
22. The method of claim 14, wherein the distortion factor is a measure of CV enlargement caused by color compensation.
23. A method of designing a probe panel for a flow cytometer, the method comprising:
identifying a first probe, a second probe, and a third probe;
identifying a plurality of possible probe panels, each possible probe panel including a combination of the first probe, the second probe, or the third probe, each probe having a possible label associated thereto;
evaluating a first possible probe panel by determining the detection limit of the first probe based on spectrum spillover effects of combination of the second probe and its associated possible label;
evaluating a second possible probe panel by determining the detection limit of the second probe based on spectrum spillover effects of the combination of the third probe and its associated possible label; and
selecting the probe panel from the plurality of possible probe panels based on the detection limits determined.
24.-27. (canceled)
28. The method of claim 23, wherein spectrum spillover effects of combination of the second probe and its associated label is determined to be zero if the coexpression pattern of antigens associated with the first probe and the second probe is mutually exclusive.
29. The method of claim 23, wherein spectrum spillover effects of combination of the second probe and its associated label is determined to be zero if the antigen associated with the second probe is a descendent of the antigen associated with the first probe.
30.-33. (canceled)

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. A method of producing salted cod roe, comprising pickling the roe with a seasoning solution by injecting the seasoning solution into the roe.
2. A method for producing salted cod roe, comprising pickling the roe with a seasoning solution by injecting the seasoning solution into the roe and then freezing the roe.
3. A method of producing salted cod roe according to claim 1, further comprising washing thawed or fresh roe with a saline solution and then injecting the seasoning solution into the roe.
4. A method of producing salted cod roe according to claim 2, further comprising washing thawed or fresh roe with a saline solution and then injecting the seasoning solution into the roe.

1. A gyroscope comprising a member for supporting a vibrator with a bonding wire, said member comprising:
a supporting plate with an opening formed therein to be positioned directly under the vibrator; and
said bonding wire comprising an end portion to be bonded with said vibrator, a fixed portion fixed on said supporting plate, a leg portion extending from said end portion and a curved portion provided between said leg portion and said fixed portion.
2. The gyroscope of claim 1, wherein a first tangential line abuts on a face of said fixed portion opposite to said vibrator, a second tangential line abuts on a face of said leg portion opposite to said vibrator at an end of said leg portion on a side of said end portion and a crossing point of said first and second tangential lines are drawn, wherein a distance \u201cL3\u201d between said crossing point and a position where said bonding wire starts to protrude from said supporting plate on said first tangential line is 10 percent or more of a distance \u201cL4\u201d between said crossing point and said end of said leg portion on the side of said end portion on said second tangential line.
3. The gyroscope of claim 1, further comprising a substrate for supporting said supporting plate, wherein a cavity communicated with said opening is provided in said substrate.
4. The gyroscope of claim 1, wherein said fixed portion of said bonding wire is fixed onto a back face of said supporting plate.
5. The gyroscope of claim 1, wherein said vibrator comprises a driving electrode for exciting driving vibration in said vibrator and a detection electrode for detecting detection vibration excited in said vibrator, wherein said bonding wire is electrically connected with one of said driving electrode and said detection electrode.
6. The gyroscope of claim 5, said vibrator further comprising a driving vibration piece with said driving electrode provided thereon, a detection vibration piece with said detection electrode provided thereon and a base portion provided between said driving vibration piece and said detection vibration piece.
7. An accelerometer comprising a member for supporting a vibrator with a bonding wire, said member comprising:
a supporting plate with an opening formed therein to be positioned directly under said vibrator; and
said bonding wire comprising an end portion to be bonded with said vibrator, a fixed portion fixed on said supporting plate, a leg portion extending from said end portion and a curved portion provided between said leg portion and said fixed portion.

The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.

1. An electrical connector, comprising:
a housing;
a conductive contact member disposed in said housing;
a biasing member disposed in said housing and connected to said contact member; and
a rotatable release member movable between first and second positions, when said release member is in said second position said biasing member secures a substantially fully inserted wire in electrical engagement with the conductive contact member and prevents removal of the inserted wire, and when said release member is in said first position said biasing member allows insertion of the wire in said housing and removal of the substantially fully inserted wire from said housing, wherein movement of said release member between said first and second positions moves said biasing member.
2. The electrical connector according to claim 1, wherein said release member is manually movable externally of said housing.
3. The electrical connector according to claim 1, wherein said housing has first and second openings for insertion of wires in a direction substantially parallel to one another.
4. The electrical connector according to claim 1, wherein said housing has first and second stop members to limit rotation of said rotatable member.
5. An electrical connector, comprising:
a housing;
a conductive contact assembly disposed in said housing and having a first contact portion for receiving a blade contact of an electrical device and a second contact portion for electrically engaging an inserted electrical wire;
a locking member movably disposed between first and second positions with respect to said second contact portion;
a biasing member disposed in said housing and disposed between said second contact portion and said locking member for biasing said locking member between said first and second positions; and
a release member rotatable between first and second positions,
when said release member is in said first position said locking member moves to said first position, compresses said biasing member and allows for substantial full insertion and removal of the substantially fully inserted electrical wire, and when said release member is in said second position said release member is spaced from said locking member and said biasing member moves said locking member to said second position, secures the inserted electrical wire between said locking member and said second contact portion and substantially prevents removal thereof.
6. The electrical connector according to claim 5, wherein said release member is manually rotatable externally of said housing.
7. The electrical connector according to claim 5, wherein said locking member moves in a direction substantially perpendicular to said second contact portion.
8. The electrical connector according to claim 5, wherein a stop member in said housing limits rotation of said release member.
9. The electrical connector according to claim 5, wherein the electrical wire is inserted through an opening in the housing.
10. The electrical connector according to claim 5, wherein said release member is spaced from the inserted wire.
11. The electrical connector according to claim 5, wherein notches in said second contact portion guide movement of said locking member between said first and second position.
12. The electrical connector according to claim 5, wherein said second contact portion passes through an opening in said locking member.
13. The electrical connector according to claim 12, wherein said locking member opening has a coined edge to facilitate engaging the inserted electrical wire.