What is claimed is:
1. A localized chemical shift correlated magnetic resonance spectroscopic sequence (L-COSY) for use in a method of whole body magnetic resonance (MR) spectroscopy, said L-COSY sequence comprising the steps of;
a) applying a pulse train of at least three high frequency (rf) pulses to localize a volume of interest, a first slice selective 900 rf pulse, a second slice selective 1800 rf pulse and a last slice selective 900 rf pulse, wherein the first and second slice selective rf pulses generate a first spin echo and the last slice selective 900 rf pulse generates a second echo that is a coherent transfer echo;
b) inserting an incremental period t1 after the first spin echo and before the last slice selective 900 pulse; and
c) detecting a MR signal during interval t2 after the last slice-selective 900 rf pulse and storing the MR signal data.
2. The L-COSY of claim 1, further comprising the steps of:
d) attaching slice-selective B0 gradient pulses to the first slice selective 900 rf pulse, the second slice selective 1800 rf pulse and the last 900 rf pulse;
e) applying slice refocusing B0 gradient pulses after the first slice selective 900 rf pulse, before the last 900 rf pulse and after the last 900 rf pulse;
f) applying B0 gradient crusher pulses before and after the second slice selective 1800 rf pulse and before and after the last 900 rf pulse
3. The L-COSY sequence of claim 2, further comprising
g) repeating steps a) to f) with different values of t1; and
h) subjecting the stored signal data to a double Fourier transformation with respect to t1 and t2 to obtain a two dimensional (2D) MR spectrum.
4. The method of claim 3, further comprising suppressing MR signals of a solvent.
5. The method of claim 4 wherein the solvent is water
6. A non-invasive method for identifying brain metabolites comprising the steps of:
a) subjecting a volume of interest to the L-COSY sequence of claim 3, wherein the volume of interest is localized within a region of the brain:identifying at least one characteristic cross peak corresponding to a brain metabolite within the 2D MR spectrum.
7. A method according to claim 6, wherein the characteristic cross peak is asymmetric with respect to the diagonal peaks
8. The method of claim 6, wherein the characteristic cross peak corresponds to a metabolite selected from the group consistintg of N-acetyl aspartate (NAA), glutamateglutamine (Glx), myo-inositol (ml), creatine (Cr), choline (Ch), aspartate (Asp), -aminobutyrate (GABA), threonine (Thr), glutathione (GSH) and macromolecules (MM).
9. The method of claim 6 wherein the region of the brain is frontal graywhite matter or occipital graywhite matter.
10. The method of claim of 6, further comprising extracting a cross-sectional one dimensional (1D) MR spectra of a metabolite from the 2D L-COSY spectrum.
11. A coherence transfer based spin-echo spectroscopy (CABINET) sequence for use as a volume localization sequence on a magnetic resonance imaging (MRI) scanner, said CABINET sequence comprising the steps of:
a) applying a pulse train of at least three high frequency (rf) pulses to localize a volume of interest, a first slice selective 900 rf pulse, a second slice selective 1800 rf pulse and a last slice selective 900 rf pulse, wherein the first and second slice selective rf pulses generate a first spin echo and the last slice selective 900 rf pulse generates a second echo that is a coherent transfer echo;
b) inserting an incremental period t1 after the first spin echo and before the last slice selective 900 pulse; and
c) detecting a MR signal during interval t2 after the last slice-selective 900 rf pulse and storing the MR signal data.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A sonotrode for mounting an antenna wire to an inlay substrate for a transponder, comprising:
a capillary having a supply end, a delivery end, and an internal bore through which the wire passes;
a clamping mechanism for holding the wire relative to the capillary;
a cutting mechanism for at least partially severing the wire;
wherein:
the cutting mechanism is disposed before the capillary.
2. The sonotrode of claim 1, wherein:
the clamping mechanism is disposed before the capillary.
3. The sonotrode of claim 1, wherein:
the cutting mechanism is disposed between the capillary and the clamping mechanism.
4. The sonotrode of claim 1, further comprising:
means for removing insulation from the wire where it is partially severed.
5. The sonotrode of claim 1, wherein:
the cutting mechanism severs the wire.
6. The sonotrode of claim 1, further comprising:
a laser for removing insulation from the wire.
7. The sonotrode of claim 6, wherein:
insulation is removed from the wire either above or below the cutting mechanism.