1. A pouch for applying a liquid, comprising:
a collapsible and flexible pouch housing for containing an amount of fluid;
an opening defined in the pouch;
an adapter in fluid communication with the pouch opening for applying fluid from the pouch to a desired location.
2. The pouch of claim 1, wherein said adapter is one of a Presta and a Schrader valve adapter for mating with the valve stem of one of a tire or of an inner tube.
3. The pouch of claim 2, wherein said fluid in said pouch is a tire sealant.
4. The pouch of claim 1, wherein said adapter includes a spoutcap, a tip attached to the pouch opening for the purpose of dispensing a fluid into a tire through a valve stem.
5. The pouch of claim 4, wherein said tip comprises of a connector tip containing any one or more from the group of a Schrader valve adapter, a Presta valve adapter for attaching to a valve stem to dispense a fluid into a tire through a valve stem.
6. The pouch of claim 1, wherein said adapter includes a hose for the purpose of dispensing a liquid into a tire through a valve stem.
7. The pouch of claim 1, wherein said adapter includes an extendable hose that is stored within said pouch and extends through the pouch opening for dispensing a liquid into a tire through a valve stem.
8. The pouch of claim 7, wherein said hose has an interior diameter sized to close seal with said valve stem when said hose is connected over said valve stem.
9. The pouch of claim 7, wherein the pouch opening and the hose are configured such that the opening wipes andor squeegees the hose as the hose is extended outside the pouch.
10. The pouch of claim 7, wherein said hose comprises a connector tip containing one or more of the group of a Schrader valve adapter, a Presta valve adapter, or both a Schrader and Presta valve adapter, and a connector tip, for attaching to a valve stem for the purpose of dispensing a liquid into a tire through a valve stem.
11. A tire sealant injector for injecting sealant into a tire, the tire sealant injector comprising:
a sealant reservoir having a chamber for receiving tire sealant, said chamber having an inside and an outside surface, said chamber having an axial length;
a tubing connector positioned at one end of said chamber;
a length of tubing having a first and second end, said first end being attached to said tubing connector;
a valve stem connector having a length, said valve stem connector having an internal bore through said length, said internal bore having a first set of female threads and a second set of female threads located within said internal bore, said first set of threads being Schrader threads and said second set of threads being Presta threads;
wherein said reservoir is formed by a flexible, collapsible pouch so that the entire contents of the reservoir can be expelled from the reservoir in one continuous compression of the flexible, collapsible pouch regardless of the amount of fluid in the reservoir;
whereby tire sealant is dispensed through said tubing connector, tubing and valve stem connector into a tire through a valve stem by compressing said pouch.
12. A fluid injector for injecting fluid into an opening, the fluid injector comprising:
a fluid reservoir having a chamber for receiving fluid, said chamber having an inside and an outside surface, said chamber;
a length of tubing having a first and second end, said first end being in fluid communication with the interior of said chamber;
wherein said reservoir is formed by a flexible, collapsible pouch so that the entire contents of the reservoir can be expelled from the reservoir in one continuous compression of the flexible, collapsible pouch regardless of the amount of fluid in the reservoir;
whereby tire sealant is dispensed through said tubing connector, tubing and valve stem connector into a tire through a valve stem by compressing said pouch.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for sensing at least one analyte in a sample comprising:
interacting the sample with a biosensor, the biosensor comprising a sensor element having a receptor site and a host molecule, wherein the host molecule interacts with the receptor site of the sensor element and the analyte as an adapter between the analyte and the receptor site so that the sensor element directly produces a detectable signal; and
detecting the signal;
wherein detection of the signal indicates the presence of said at least one analyte.
2. A method for sensing at least one analyte in a sample comprising:
interacting the sample with a biosensor, the biosensor comprising a sensor element having a receptor site and a host molecule, wherein the host molecule interacts with the receptor site of the sensor element and the analyte as a carrier to deliver the analyte to the receptor site so that the sensor element directly produces a detectable signal; and
detecting the signal;
wherein detection of the signal indicates the presence of said at least one analyte.
3. The method of any one of claim 1 or 2 wherein interacting comprises stochastic sensing.
4. The method of any one of claim 1 or 2 wherein the host molecule is non-covalently attached to the receptor site.
5. The method of any one of claim 1 or 2 wherein the host molecule is covalently attached to the receptor site.
6. The method of any one of claim 1 or 2 wherein the biosensor further comprises a bilayer and the sensor element comprises a channel disposed in the bilayer and coupled to the receptor site in manner that allows interaction of the host molecule with the receptor site to produce a signal using the channel.
7. The method of any one of claim 1 or 2 wherein the biosensor further comprises a bilayer apparatus, the bilayer apparatus comprising a bilayer separating the bilayer apparatus into a first compartment and a second compartment and wherein the sensor element is disposed in the bilayer so that it forms a channel in the bilayer.
8. The method of claim 7 wherein the sensor element is disposed in the first compartment so that it forms a channel in the bilayer and the host molecule is disposed in the second compartment.
9. The method of claim 7 wherein the sensor element is disposed in the first compartment so that it forms a channel in the bilayer and the host molecule is disposed in the first compartment, the second compartment or both compartments.
10. The method of any one of claim 1 or 2 wherein the biosensor further comprises a bilayer apparatus, the bilayer apparatus comprising a bilayer separating the bilayer apparatus into a first compartment and a second compartment and wherein the sensor element is added to the first compartment and stirred to form a channel in the bilayer, and the step of interacting the sample with the biosensor comprises adding the host molecule and the sample to the first compartment, the second compartment, or both compartments.
11. The method of any one of claim 1 or 2 further comprising identifying the analyte using the signal.
12. The method of any one of claim 1 or 2 further comprising quantitating the analyte using the signal.
13. The method of any one of claim 1 or 2 wherein the host molecule is a cyclodextrin.
14. The method of claim 13 wherein the cyclodextrin is \u03b2-cyclodextrin (\u03b2CD).
15. The method of claim 13 wherein the cyclodextrin is s7\u03b2CD.
16. The method of any one of claim 1 or 2 wherein the sensor element is a protein.
17. The method of claim 16 wherein the protein is selected from the group consisting of a transmembrane pore, an enzyme, an antibody and a receptor.
18. The method of any one of claim 1 or 2 wherein the sensor element comprises a pore.
19. The method of claim 18 wherein the sensor element comprises a genetically engineered transmembrane protein pore.
20. The method of claim 18 wherein the sensor element is an \u03b1-Hemolysin (HL) pore.
21. The method of claim 20 wherein the sensor element is a wild-type \u03b1-Hemolysin (\u03b1HL) pore.
22. The method of claim 20 wherein the sensor element is a genetically engineered or mutant \u03b1-Hemolysin (\u03b1HL) pore.
23. The method of any one claim 1 or 2 wherein the biosensor senses at least two analytes.
24. The method of any one claim 1 or 2 wherein the signal comprises a change in electrical current.
25. The method of claim 24 wherein the signal comprises a change in the magnitude and duration of the change in the current.
26. The method of any one of claim 1 or 2 wherein the analyte is an organic molecule.
27. The method of any one claim 1 or 2 wherein the analyte is not charged.
28. The method of any one claim 1 or 2, wherein the signal is selected from the group consisting of a change in fluorescence, a change in electrical current and a change in force.