1. A web medium supply for a web medium on a core, the web medium supply comprising;
a frame having a first mounting support and a second mounting support that are positioned along an axis of rotation and separated by a separation distance during the supply of the web;
a first core mounting having a first surface that is rotatably supportable by the first mounting support about the axis of rotation and a first engagement end to which a first end of a core can be mounted and a first engagement surface through which a first force urging the core to rotate can be transmitted to the core to urge the core to rotate with the first core mounting; and
a second core mounting having a second surface that is rotatably supportable by the second mounting support about the axis of rotation and a second engagement end to which a second end of the core can be mounted and a second engagement surface through which a second force urging the second core to rotate can be transmitted to the core to urge the core to rotate with the second core mounting; and
a drive transmission having an input end to receive an input force, a first output mechanically linked for movement with the first core mounting and a second output mechanically linked for movement with the second core mounting with the drive transmission mechanically linking the input end to the first output and to the second output and distributing an amount of force supplied at the input end between the first force and the second force, such that the first force and the second force can, in combination, rotate the first core mounting, second core mounting, the core and any web wound thereon against an inertial load of the core and web;
wherein both the first force and the second force are less than a third force that is sufficient to drive an alternative core against the inertial load from one driven end, and wherein the core has a first yield strength at the first end and a second yield strength at the second end that are each less than a third yield strength that is required to receive the third force at the driven end of the alternative core.
2. The web medium supply claim 1, wherein a volume of the core providing the first yield strength and the second yield strength is less than a volume of the alternative core providing the third yield strength at the driven end so that more volume is available in the web medium supply for web medium than would be available if the alternative core is used.
3. The web medium supply of claim 1, wherein a radius of the core that provides the first yield strength and the second yield strength is less than a radius of the alternative core providing the third yield strength at a driven end of the alternative core, so that a volume of web supplied by the core creates less angular momentum than the same volume of web would create if supplied by the alternative core.
4. The web medium supply of claim 1, wherein a radius of the core providing the first yield strength and the second yield strength is less than a radius of the alternative core providing the third yield strength, so that the volume of a web medium supply in which the core operates can be made smaller than the volume of a web medium supply in which the alternative core operates still supplying a given volume web.
5. The web medium supply of claim 1, wherein a volume of the shaft of the core having the first yield strength and second yield strength is smaller than a volume of an alternative shaft of the alternative core that provides the third yield strength while using the same material for fabrication of the core and for fabrication of the alternative core.
6. The web medium supply of claim 1, wherein the core can be made using a first material that provides the first yield strength and second yield strength in a determined configuration, but must be made using a second material that is more dense than the first material to provide the third yield strength to make the alternative core in the determined configuration.
7. The web medium supply of claim 1, wherein the core can be made from a first material that provides the first yield strength and second yield strength in a determined configuration, but must be made using a second material that is more rigid than the first material to provide the third yield strength to make the alternative core in the determined configuration.
8. The web medium supply of claim 1, wherein the drive transmission causes the first output and the second output to cause the first end of the core and the second end of the core to remain within a range of rotational positions relative to each other with the range being defined so that differences in the rotational positions of the first end and second end create a determined range of shear stress in the core.
9. The web medium supply of claim 1, wherein the core has an interior passageway from the first end of the core to the second end of the core and where the web medium supply further comprises the steps of mounting a first end of the core to a first core mounting and a second end of the core to a second core mounting and mechanically linking the first core mounting to the second core mounting within the interior passageway of the core such that a portion of an input force can be transferred from a first end of the core to a second end of the core through the mechanical linkage of the first core mounting and the second core mounting.
10. The web medium supply of claim 1, wherein at least one of the first core mounting and the second core mounting are movable along the axis of rotation relative to between a range of driving positions and a range of slip positions, and further comprising a biasing member urging the one of the first core mounting and the second core mounting toward the range of mounting positions.
11. The web medium supply of claim 10, wherein the bias on the at least one of the first core mounting and the second core mounting is such that the core and the mounting slip relative each other when a torque in excess of a predetermined level is applied at the intersection.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A gene therapy method for a human subject, comprising (A) providing a composition comprised of a vector and a polynucleotide encoding a therapeutic polypeptide to which said subject is immunologically competent and (B) administering said composition to the subject, such that (i) said therapeutic polypeptide is expressed selectively in hepatocytes of the subject and thereafter (ii) said subject fails to generate a medically-significant immune response to the expressed therapeutic polypeptide.
2. The method of claim 1, wherein said composition further comprises a pharmaceutically suitable excipient.
3. The method of claim 1, wherein said composition is administered intravenously.
4. The method of claim 3, wherein said intravenous administration is effected through the group consisting of the portal vein, mesenteric vein and hepatic artery of said subject.
5. The method of claim 1, wherein said polynucleotide is operably linked to a liver-specific promoter.
6. The method of claim 5, wherein said liver-specific promoter is a human 1-antitrypsin promoter.
7. The method of claim 1, wherein said polynucleotide is operably linked to ubiquitous promoter.
8. The method of claim 1, wherein said vector can be selected from the group consisting of a plasmid, an adenovirus vector, an adeno-associated virus vector, herpes simplex virus vector, lentivirus vector and retrovirus vector.
9. The method of claim 8, wherein said vector is an adeno-associated virus vector.
10. The method of claim 1, wherein said therapeutic polypeptide modulates the blood clotting or coagulation cascade.
11. The method of claim 10, wherein said subject suffers from hemophilia.
12. The method of claim 11, wherein said subject suffers from hemophilia B and said therapeutic polypeptide that modulates the blood clotting or coagulation cascade is factor IX.
13. The method of claim 1, exclusive of using an immunomodulator.
14. The method of claim 12, exclusive of using an immunomodulator.
15. The method of claim 1, wherein said composition is administered before said subject has exhibited immune intolerance to said therapeutic polypeptide.
16. The method of claim 10, wherein said composition is administered before said subject has exhibited immune intolerance to functional factor IX.