1. A stop sign brake light device, comprising:
an octagonal-shaped main body;
a border that is disposed along a periphery of the main body;
a word STOP that is positioned along a central portion of the main body;
a plurality of lighted elements that are disposed along an entirety of the reflective border and the word STOP;
a wiring harness that is configured to receive power from a vehicle power system; and
a controller that is in communication with each of the wiring harness and the plurality of lighted elements.
2. The device of claim 1, wherein each of the reflective border and the word STOP include a red color.
3. The device of claim 1, wherein the border is coated with a reflective material.
4. The device of claim 1, wherein the word STOP is coated with a reflective material.
5. The device of claim 1, wherein each of the lighted elements include a light emitting diode.
6. The device of claim 1, wherein the plurality of lighted elements generate red light.
7. The device of claim 1, wherein the plurality of lighted elements generate at least two different colors of light.
8. The device of claim 1, wherein the controller further includes functionality for causing one or more of the plurality of lighted elements to blink.
9. The device of claim 1, wherein the controller further includes functionality for causing two or more of the plurality of lighted elements to light in a pattern.
10. The device of claim 1, further comprising an electrical connector that is disposed along a distal end of the wiring harness, said electrical connector functioning to engage a vehicle brake electrical wire.
11. The device of claim 1, wherein the main body includes a dimension of approximately 8\u2033\xd78\u2033 for use on at least one of a motorcycle and a passenger vehicle.
12. The device of claim 1, wherein the main body includes a dimension of between approximately 16\u2033\xd716\u2033 and 24\u2033\xd724\u2033 for use on a recreational vehicle.
13. The device of claim 1, wherein the main body includes a dimension of approximately 48\u2033\xd748\u2033 for use on a commercial vehicle.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A live attenuated dengue-1 virus strain which comprises sequence SEQ ID NO: 3 wherein at least nucleotides at positions 5962 and 7947 are mutated, with the proviso that the following nucleotides are not mutated: 1323, 1541, 1543, 1545, 1567, 1608, 2363, 2695, 2782, 5063, 6048, 6806, 7330, and 9445.
2. The dengue-1 virus strain according to claim 1, wherein the nucleotide at position 2719 is further mutated.
3. The dengue-1 virus strain according to claim 1, wherein SEQ ID NO: 3 comprises the mutations 2719 G>A, 5962 C>A, and 7947 A>G.
4. The dengue-1 virus strain according to claim 1, which further comprise a substitution of one or more nucleotides in a given codon position which results in no alteration in the amino acid encoded at that position.
5. The dengue-1 virus strain according to claim 1, which comprises SEQ ID NO: 1.
6. An immunogenic composition comprising a live attenuated dengue-1 virus strain which comprises sequence SEQ ID NO: 3 wherein at least nucleotides at positions 5962 and 7947 are mutated, with the proviso that the following nucleotides are not mutated: 1323, 1541, 1543, 1545, 1567, 1608, 2363, 2695, 2782, 5063, 6048, 6806, 7330, and 9445, in a pharmaceutically acceptable carrier.
7. The immunogenic composition according to claim 6, wherein said live attenuated dengue-1 virus strain comprises SEQ ID NO: 3 in which A at position 2719 is substituted for G, A at position 5962 is substituted for C, and G at position 7947 is substituted for A.
8. The immunogenic composition according to claim 6, wherein said live attenuated dengue-1 virus strain further comprises a substitution of one or more nucleotides in a given codon position which results in no alteration in the amino acid encoded at that position.
9. The immunogenic composition according to claim 6, wherein said live attenuated dengue-1 virus strain comprises SEQ ID NO: 1.
10. The immunogenic composition according to claim 6, which further comprises at least one live attenuated dengue virus selected from the group consisting of serotype 2, serotype 3, and serotype 4.