1. A kit for assaying a biological sample potentially containing two or more analytes, said kit comprising:
(a) a stationary solid phase support with immobilized receptors for binding thereto said analytes and
(b) two or more different types of sensitized microcapsules, each different type of said sensitized microcapsules having a surface membrane and an interior volume, said surface membrane of said each different type of said sensitized microcapsules having an incorporated receptor that specifically binds to a particular one of said analytes and said interior volume of said each different type of said sensitized microcapsules includes a unique first member of a binding pair, each said unique first member of a binding pair in said each different type of said sensitized microcapsules is different and specifically binds a second member of the binding pair.
2. The kit according to claim 1 further comprising:
a disrupting agent suitable to disrupt said sensitized microcapsules and release each said unique first member of the binding pair from each different type of said sensitized microcapsules when said analytes are present in the biological sample.
3. The kit according to claim 1 further comprising:
a device comprising:
means for immobilizing each said unique first member of the binding pair released from said each different type of said sensitized microcapsules in spatially separated locations; and
means for detecting each said unique first member of the binding pair released from said each different type of said sensitized microcapsules immobilized in said spatially separated locations.
4. The kit according to claim 1, wherein said unique first member of the binding pair comprises a tag.
5. The kit according to claim 4, wherein said tag is an electrochemically detectable species, a colorimetrically detectable species, a fluorescent species, an oligomarker, an oligonucleotide molecule, or an oligonucleotide molecule conjugated to a fluorophore tag, an electrochemical tag, a quantum dot tag, or a dye tag.
6. The kit according to claim 3, wherein said means for detecting each said unique first member of the binding pair are colorimetric, fluorescent, or electrochemical means of detection.
7. The kit according to claim 1, wherein said unique first member of the binding pair is an antigen, a hapten, an antibody, a cytokine, a chemokine, an enzyme, a drug, a protein, a biomarker, a DNA, or an RNA.
8. The kit according to claim 1, wherein said second member of the binding pair is an antigen, a hapten, an antibody, a cytokine, a chemokine, an enzyme, a drug, a protein, a biomarker, a DNA, or an RNA.
9. The kit according to claim 3, wherein said unique first member of the binding pair is an oligonucleotide molecule.
10. The kit according to claim 1, wherein said microcapsules are liposomes or polymer based microspheres.
11. The kit according to claim 3, wherein said means for immobilizing each said unique first member of the binding pair released from said each different type of said microcapsules comprises the second members of the binding pair which are immobilized.
12. The kit according to claim 9, wherein said means for immobilizing each said unique first member of the binding pair released from said each different type of said sensitized microcapsules comprises an array of immobilized oligonucleotides complementary to said oligonucleotide molecules, wherein each said oligonucleotide molecule released from said each different type of said sensitized microcapsules is brought into contact with said array and hybridizes with said immobilized oligonucleotides.
13. A kit for assaying a biological sample potentially containing at least one analyte, said kit comprising:
(a) a first sensitized microcapsule, said first sensitized microcapsule having a surface membrane and an interior volume, said surface membrane of said first sensitized microcapsule having an incorporated receptor that specifically binds to said analyte and said interior volume of said first sensitized microcapsule includes a unique first member of a binding pair that specifically binds a second member of the binding pair;
(b) an immobilized binder of said analyte;
(c) a second sensitized microcapsule filled with tags, said second sensitized microcapsule having a surface membrane and an interior volume, said surface membrane of said second sensitized microcapsule having an incorporated second member of the binding pair for specifically binding to said first member of the binding pair; and
(d) an immobilized second member of the binding pair for specifically binding to said first member of the binding pair.
14. The kit according to claim 13 further comprising:
a disrupting agent suitable to disrupt said first sensitized microcapsule and release said first member of the binding pair from said first sensitized microcapsule when said analyte is present in the biological sample and
a disrupting agent suitable to disrupt said second sensitized microcapsule and releasing said tags when said analyte is present in the biological sample.
15. The kit according to claim 13 further comprising:
release said first member of the binding pair from the first sensitized microcapsule when said analyte is present in the biological sample and to disrupt said second sensitized microcapsule and release said tags when said analyte is present in the biological sample.
16. The kit according to claim 13, wherein said tags are electrochemically detectable species, colorimetrically detectable species, fluorescent species, oligomarkers, oligonucleotide molecules, or oligonucleotide molecules conjugated to a fluorophore tag, an electrochemical tag, a quantum dot tag, or a dye tag.
17. The kit according to claim 13, wherein said first member of the binding pair is an antigen, a hapten, an antibody, a cytokine, a chemokine, an enzyme, a drug, a protein, a biomarker, a DNA, or an RNA.
18. The kit according to claim 13, wherein said second member of the binding pair is an antigen, a hapten, an antibody, a cytokine, a chemokine, an enzyme, a drug, a protein, a biomarker, a DNA, or an RNA.
19. The kit according to claim 13, wherein said first member of the binding pair is an oligonucleotide molecule.
20. The kit according to claim 13, wherein said microcapsules are liposomes or polymer based microspheres.
21. The kit according to claim 19, wherein said immobilized second member of the binding pair comprises oligonucleotides immobilized on an array which are complementary to said oligonucleotide molecules.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A tilting apparatus in a monitor comprising:
a monitor main body;
a base member;
a monitor hinge supporting the monitor main body to be tilted against the base member within a predetermined angle; and
a shield rotatably connected to the monitor main body to shield a gap formed between the monitor main body and the base member when the monitor main body is tilted up with respect to the base member,
wherein the shield comprises:
a shield main body connected to the monitor main body; and
a rotatable body rotatably connected to the shield main body to shield the gap between the monitor main body and the base member.
2. The monitor according to claim 1, wherein the shield main body comprises a main part having a plate shape and combined to a rear side of the monitor main body and a pair of main part supporting parts provided in opposite ends of the main part and being perpendicular to a surface of the main part, and the rotatable body comprises:
a pair of rotatable body supporting parts rotataby coupled to the main part supporting parts, respectively; and
a shielding part provided between the rotatable body supporting parts to shield the gap between the monitor main body and the base member.
3. The monitor according to claim 2, wherein the shield includes a spring member elastically separating the rotatable body from the shield main body.
4. The monitor according to claim, wherein the spring member comprises a flat spring having a first end combined to the main part and a second end contacting the shielding part.
5. The monitor according to claim 2, wherein one of the main part supporting part and the rotatable body supporting part comprises a hinge hole, and the other one of the main part supporting part and the rotatable body supporting part comprises a hinge projection in correspondence to the hinge hole.
6. The monitor according to claim 5, wherein the shield includes a spring member elastically separating a rotatable body from the shield main body.
7. The monitor according to claim 6, wherein the spring member comprises a flat spring having a first end combined to the main part and a second end contacting the shielding part.
8. The monitor according to claim 1, wherein the monitor hinge comprises:
a link member provided between the monitor main body and the base member and rotatably combined with the monitor main body.
9. The monitor according to clam 8, herein the monitor comprises a cable coupled to the monitor main body and disposed in the gap, and the link member accommodates the cable therein.
10. The monitor according to claim 8, wherein the monitor hinge comprises:
a pair of monitor brackets spaced from each other, fastened to the monitor main body, and rotatably connected to the link member.
11. The monitor according to claim 10, wherein the monitor hinge comprises:
a tilt limiting unit limiting a monitor main body against the link member.
12. The monitor according to claim 11, wherein the tilt limiting unit comprises:
a rotation limiting washer formed with a pair of guide projections protruding and spaced from each other; and
a rotation limiting projection incorporated with one of the monitor brackets, disposed between the guide projections, and rotating within a predetermined angle formed by the guide projections.
13. A monitor, comprising
a monitor main body;
a base member;
a monitor hinge mounted on the base member and rotatably connected to the monitor main body to form an angle with the monitor main body; and
a shield movably connected to one of the base member, the monitor hinge, and the monitor main body to shield a gap formed between the monitor main body and the base member in response to a tilting movement of the monitor main body, wherein the shield comprises:
a shield main body having a main part mounted on the one of the base member, the monitor hinge, and the monitor main body, a main part supporting part extended from the main part, a hinge projection formed on the main part supporting part; and
a rotatable body having a shield part and a hinge hole formed on the shield part to receive the hinge projection of the main part supporting part, the shield part rotating to shield the gap in response to the tilting movement of the monitor main body.
14. The monitor according to claim 13, wherein the rotatable body rotates about an axis passing through one of the hinge hole and the hinge projection.
15. The monitor according to claim 13, wherein the shield comprises:
a spring holder formed on the main part of the shield main body; and
a flat spring disposed in the holder and extended toward the shield part of the rotatable body to bias the rotatable body with respect to the shield main body to shield the gap in response to the tilting movement of the monitor main body.
16. The monitor according to claim 13, wherein the shield comprises: a flat spring disposed between the shield and the monitor main body or between the shield and the base member to bias the shield to move to shield the gap in response to the tilting movement of the monitor main body.
17. The monitor according to claim 13, wherein the monitor main body forms a first angle and a second angle greater than the first angle with the base member to form the gap, and the shield moves from a first position to a second position to shield the gap in response to the tilting movement when the monitor main body is tilted to form the second angle with the base member.
18. The monitor according to claim 13, wherein the monitor hinge comprises:
a link member;
a lower end part formed on the link member and coupled to the base member; and
a monitor bracket fixedly connected to the monitor main body and rotatably connected to the link member.
19. The monitor according to claim 18, wherein the monitor bracket comprises a hinge hole, and the monitor hinge comprises:
an upper end part formed on the link member;
a non-circular shaped hinge hole formed on the upper end part of the link member; and
a hinge bolt having a non-circular shaped spline part and a rod to be connected to the non-circular shaped hinge hole of the upper end part of the link member and the hinge hole of the monitor bracket, respectively.
20. The monitor according to claim 19, wherein the monitor hinge comprises a hinge bolt, and the hinge bolt comprises a threaded portion formed on the rod to be coupled to the hinge nut when the non-circular shaped spline part of the hinge bolt is disposed in the non-circular shaped hinge hole and when the rod is disposed in the hinge hole of the monitor bracket.
21. The monitor according to claim 19, wherein the monitor hinge comprises a rotating limiting washer having a non-circular shaped washer hole and guide projections spaced-apart from each other and disposed around the non-circular shaped washer hole, end the monitor bracket comprises:
a rotation limiting projection disposed to rotate between the guide projections of the rotating limiting washer to limit the angle between the base member and the monitor main body.
22. The monitor according to claim 21, wherein the rotating limiting washer is disposed between the upper end part and the monitor bracket, and the non-circular shaped spline part of the hinge bolt is inserted into the non-circular shaped hinge hole of the upper end part and the rotation limiting washer while the rod of the hinge bolt is inserted Into the hinge hole of the monitor bracket.
23. The monitor according to claim 21, wherein the monitor bracket rotates with respect to the upper end part of the monitor hinge and the rotation limiting washer.
24. The monitor according to claim 21, wherein the rotation limiting washer does not rotate with respect to the upper end part of the monitor hinge.
25. The monitor according to claim 13, wherein the angle formed by the monitor main body and the base member is in a range between 0\xb0 and 40\xb0 inclusive.