1. A lens blank coming from a mold having a sheet insert placed therein prior to injection, the blank including peripheral ejector marks, said marks lying outside the insert.
2. A blank according to claim 1, wherein each mark is carried by a tab having extra thickness on the same side as the insert, and a recess in its other side, the recess forming an element for centering the spacer of an adjacent blank.
3. A method of fabricating a blank according to claim 1 by injection molding, using a mold that opens in two parts that define between them at least one cavity, one of the parts being provided with a plurality of ejectors movable perpendicularly to the closure plane of the mold, the method consisting, starting from the open mold, in:
placing the ejectors in the ejection position;
engaging the sheet insert between the ejectors and retaining it between them by elastic wedging;
closing the mold while retracting the ejectors into the corresponding part;
injecting a thermoplastic material into the mold;
opening the mold after the thermoplastic material has hardened;
placing the ejectors back in the ejection position; and
extracting the composite blank as obtained in this way.
4. A mold for implementing the method according to claim 3, wherein each cavity has four movable ejectors.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for discriminating a target base carried by a target DNA, comprising:
(1) a DNA elongation step comprising
(a) binding to said target DNA a test allele specific primer,
wherein said target DNA comprises the sequence 3\u2032-(C-C-S1)-5\u2032, S1 being the target base, and
wherein said test allele specific primer comprises the sequence 5\u2032-(B3-B2-S1\u2032)-3\u2032, (B3, B2) being bases selected from the group consisting of (A, T), (T, A), (T, T) and (C, T),
wherein S1\u2032 is the 3\u2032 terminal base of the test allele specific primer and is complimentary to the base predicted to be S1; and
(b) causing a test DNA elongation reaction; and
(2) a discrimination step comprising measuring the efficiency of said DNA elongation to discriminate that said target base is the same as predicted, or that said target base is distinct from said predicted base.
2. The method according to claim 1 wherein said DNA elongation reaction is a primer extension reaction.
3. The method according to claim 1 wherein said DNA elongation reaction is a primer extension reaction relied solely on said allele specific primer.
4. The method according to claim 2 wherein said efficiency is examined by determining the concentration of pyrophosphate produced by said primer extension reaction.
5. The method according to claim 4 wherein said concentration of pyrophosphate is detected in terms of luminescence intensity.
6. The method according to claim 1 wherein said DNA elongation reaction is a PCR reaction.
7. The method according to claim 6 wherein said efficiency is examined by measuring the concentration of the amplified DNA produced by said PCR reaction with an electrophoretic method.