1. A polynucleotide thermal melt calibrator having a stem-loop configuration, comprising:
a first stem region having a first nucleotide sequence;
a second stem region having a second nucleotide sequence, wherein the second nucleotide sequence is sufficiently complimentary to the first nucleotide sequence to at least substantially hybridize and at least substantially denature across a temperature range of about 10\xb0 C. or less;
a loop region joining the first stem region and the second stem region, the loop region having a loop sequence and a loop length to allow hybridization of the first stem region and the second stem region;
a hybridization indicator associated with at least one of the first stem region, the second stem region, or the loop region.
2. The calibrator of claim 1, wherein the second nucleotide sequence is sufficiently complimentary to the first nucleotide sequence to substantially hybridize and substantially denature across a temperature range of about 5\xb0 C. or less.
3. The calibrator of claim 1, wherein the first nucleotide sequence is selected from the group consisting of SEQ ID 001, SEQ ID 002, SEQ ID 005, SEQ ID 006, SEQ ID 009, SEQ ID 010, SEQ ID 012, and SEQ ID 013.
4. The calibrator of claim 1, wherein the calibrator has a melt profile with a peak at a temperature of from about 60\xb0 C. to about 75\xb0 C.
5. The calibrator of claim 4, wherein the calibrator has a melt profile with a peak at a temperature of from about 64\xb0 C. to about 70\xb0 C.
6. The calibrator of claim 4, wherein the first nucleotide sequence is selected from the group consisting of SEQ ID 001, SEQ ID 002, SEQ ID 009, and SEQ ID 010.
7. The calibrator of claim 6, wherein the loop sequence is SEQ ID 003.
8. The calibrator of claim 4, wherein the first nucleotide sequence is SEQ ID 001, the second nucleotide sequence is SEQ ID 002, and the loop sequence is SEQ ID 003.
9. The calibrator of claim 1, wherein the calibrator has a melt profile with a peak at a temperature of from about 85\xb0 C. to about 105\xb0 C.
10. The calibrator of claim 9, wherein the calibrator has a melt profile with a peak at a temperature of from about 90\xb0 C. to about 98\xb0 C.
11. The calibrator of claim 9, wherein the first nucleotide sequence is selected from the group consisting of SEQ ID 005 and SEQ ID 006.
12. The calibrator of claim 11, wherein the loop sequence is SEQ ID 007.
13. The calibrator of claim 9, wherein the first nucleotide sequence is SEQ ID 005, the second nucleotide sequence is SEQ ID 006, and the loop sequence is SEQ ID 007.
14. An assay composition, comprising:
a sample of genomic DNA;
at least one pair of primers specific to a DNA sequence of interest; and
a polynucleotide thermal melt calibrator as in claim 1.
15. The composition of claim 14, wherein the polynucleotide thermal melt calibrator is SEQ ID 004.
16. The composition of claim 14, wherein the polynucleotide thermal melt calibrator is SEQ ID 008.
17. The composition of claim 14, wherein the polynucleotide thermal melt calibrator is SEQ ID 011.
18. The composition of claim 14, wherein the polynucleotide thermal melt calibrator is SEQ ID 014.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A straddle-type four wheeled all terrain vehicle comprising:
a vehicle body;
a straddle-type seat provided on an upper portion of the vehicle body;
a bar-type handle having a steering shaft and provided forward of the seat;
a vehicle body cover covering a portion of the vehicle body including the steering shaft from above, the steering shaft penetrating through the vehicle body cover; and
a belt converter, wherein
an opening of the vehicle body cover through which the steering shaft passes is an intake port of a cooling air into the belt converter.
2. The straddle-type four wheeled all terrain vehicle according to claim 1, wherein a relay chamber is formed inside of the vehicle body cover such that the relay chamber communicates with the opening, and
a cooling air intake port of the belt converter is opened in the relay chamber.
3. The straddle-type four wheeled all terrain vehicle according to claim 2, wherein a portion of the vehicle body cover around the opening is raised to be formed into a swelled portion, and
the relay chamber is structured to have a chamber wall including one part constituted by a portion of the swelled portion including the opening and the other part through which the steering shaft and a cooling air intake duct having the cooling air intake port of the belt converter penetrate.
4. The straddle-type four wheeled all terrain vehicle according to claim 3, wherein a portion of the other part of the chamber wall through which at least the steering shaft and the cooling air intake duct of the belt converter penetrate is comprised of a flexible plate member.
5. The straddle-type four wheeled all terrain vehicle according to claim 4, wherein the swelled portion is configured such that front and side portions around the opening is raised and the swelled portion extends rearwardly,
the relay chamber is structured such that a portion of the swelled portion defines one part of the chamber wall of the relay chamber,
a front wall member, a pair of side wall members, a flexible plate member, and a seal structure define the other part of the chamber wall,
the front wall member and the pair of side wall members are downwardly protruded at a front end portion and side end portions of the swelled portion that is located forward and side of the opening in an inner face of the vehicle body cover such that the front wall member and the side wall members form an enclosure,
the flexible plate member substantially defines a bottom and a rear of a space covered by the portion of the swelled portion that is located forward and side of the opening,
the seal structure is formed between a front end of the flexible member and a lower end of the front wall member, and
the steering shaft and the cooling air intake duct of the belt converter penetrate through the flexible plate member.
6. The straddle-type four wheeled all terrain vehicle according to claim 5, wherein the seal structure is formed such that a support member being a frame member of the vehicle body extends along a lower end of the front wall member as having a clearance between the front wall member and the support member,
a cushion member is provided on the support member to fill the clearance, and a front end of the flexible plate member is connected to the support member.