1. A molybdated succinimide complex prepared by a process which comprises (a) reacting a succinimide of a polyamine of formula I:
wherein R is a hydrocarbon radical having a number average molecular weight of about 500 to about 5,000, a and b are independently 2 or 3, and x is 0 to 10, with an ethylenically unsaturated carboxylic acid or anhydride thereof, in a charge mole ratio of the ethylenically unsaturated carboxylic acid anhydride to the succinimide of formula I of about 0.9:1 to about 1.05:1; and (b) reacting the succinimide product of step (a) with an acidic molybdenum compound.
2. The molybdated succinimide complex of claim 1, wherein R is a hydrocarbon radical having a number average molecular weight of about 700 to about 2,500.
3. The molybdated succinimide complex of claim 1, wherein R is a hydrocarbon radical having a number average molecular weight of about 710 to about 1,100.
4. The molybdated succinimide complex of claim 1, wherein R is a polyisobutenyl radical.
5. The molybdated succinimide complex of claim 1, wherein R is a polyisobutenyl radical having a number average molecular weight of about 700 to about 2,500.
6. The molybdated succinimide complex of claim 1, wherein R is a polyisobutenyl radical having a number average molecular weight of about 710 to about 1,100.
7. The molybdated succinimide complex of claim 1, wherein a and b are each 2, and x is 2 to 5.
8. The molybdated succinimide complex of claim 4, wherein a and b are each 2, and x is 2 to 5.
9. The molybdated succinimide complex of claim 1, wherein the ethylenically unsaturated carboxylic acid anhydride is an ethylenically unsaturated monocarboxylic acid anhydride.
10. (canceled)
11. The molybdated succinimide complex of claim 1, wherein the ethylenically unsaturated carboxylic acid anhydride is an ethylenically unsaturated dicarboxylic acid.
12. The molybdated succinimide complex of claim 11, wherein the ethylenically unsaturated dicarboxylic acid or anhydride is selected from the group consisting of maleic anhydride, citraconic anhydride, itaconic anhydride and mixtures thereof.
13. The molybdated succinimide complex of claim 1, wherein the acidic molybdenum compound is selected from the group consisting of molybdenum oxide, molybdic acid, ammonium molybdate, sodium molybdate, potassium molybdates, hydrogen sodium molybdate, MoOCl4, MoO2Br2, Mo2O3Cl6, molybdenum trioxide and mixtures thereof.
14. The molybdated succinimide complex of claim 1, wherein the ethylenically unsaturated carboxylic acid anhydride is maleic anhydride and the acidic molybdenum compound is molybdenum trioxide.
15. The molybdated succinimide complex of claim 4, wherein the ethylenically unsaturated carboxylic acid anhydride is maleic anhydride and the acidic molybdenum compound is molybdenum trioxide.
16. The molybdated succinimide complex of claim 1, wherein the molar ratio of the molybdenum compound to the succinimide product of step (a) is about 0.1:1 to about 2:1.
17. The molybdated succinimide complex of claim 1, wherein the molar ratio of the molybdenum compound to the succinimide product of step (a) is about 0.5:1 to about 1.5:1.
18. A process for preparing a molybdated succinimide complex, the process comprising (a) reacting a succinimide of a polyamine of formula I:
wherein R is a hydrocarbon radical having a number average molecular weight of about 500 to about 5,000, a and b are independently 2 or 3, and x is 0 to 10, with an ethylenically unsaturated carboxylic acid or anhydride thereof, in a charge mole ratio of the ethylenically unsaturated carboxylic acid anhydride to the succinimide of formula I of about 0.9:1 to about 1.05:1; and (b) reacting the succinimide product of step (a) with an acidic molybdenum compound.
19. The process of claim 18, wherein R is a hydrocarbon radical having a number average molecular weight of about 700 to about 2,500.
20. The process of claim 18, wherein R is a hydrocarbon radical having a number average molecular weight of about 710 to about 1,100.
21. The process of claim 18, wherein R is a polyisobutenyl radical.
22. The process of claim 18, wherein R is a polyisobutenyl radical having a number average molecular weight of about 700 to about 2,500.
23. The process of claim 18, wherein R is a polyisobutenyl radical having a number average molecular weight of about 710 to about 1,100.
24. The process of claim 18, wherein the ethylenically unsaturated carboxylic acid anhydride is an ethylenically unsaturated monocarboxylic acid anhydride.
25. (canceled)
26. The process of claim 18, wherein the ethylenically unsaturated carboxylic acid anhydride is an ethylenically unsaturated dicarboxylic acid anhydride.
27. The process of claim 26, wherein the ethylenically unsaturated dicarboxylic acid anhydride is selected from the group consisting of maleic anhydride, citraconic anhydride, itaconic anhydride and mixtures thereof.
28. The process of claim 18, wherein the acidic molybdenum compound is selected from the group consisting of molybdenum oxide, molybdic acid, ammonium molybdate, sodium molybdate, potassium molybdates, hydrogen sodium molybdate, MoOCl4, MoO2Br2, Mo2O3Cl6, molybdenum trioxide and mixtures thereof.
29. The process of claim 18, wherein the ethylenically unsaturated carboxylic acid of anhydride is maleic anhydride and the acidic molybdenum compound is molybdenum trioxide.
30. The process of claim 18, wherein the molar ratio of the molybdenum compound to the succinimide product of step (a) is about 0.1:1 to about 2:1.
31. The process of claim 18, wherein the molar ratio of the molybdenum compound to the succinimide product of step (a) is about 0.5:1 to about 1.5:1.
32. A lubricating oil composition comprising (a) a major amount of a base oil of lubricating viscosity; and (b) a minor amount of a molybdated succinimide complex prepared by a process which comprises (i) reacting a succinimide of a polyamine of formula I:
wherein R is a hydrocarbon radical having a number average molecular weight of about 500 to about 5,000, a and b are independently 2 or 3, and x is 0 to 10, with an ethylenically unsaturated carboxylic acid or anhydride thereof, in a charge mole ratio of the ethylenically unsaturated carboxylic acid anhydride to the succinimide of formula I of about 0.9:1 to about 1.05:1; and (ii) reacting the succinimide product of step (i) with an acidic molybdenum compound.
33. The lubricating oil composition of claim 32, wherein the base oil of lubricating viscosity is comprised of a mineral base oil.
34. The lubricating oil composition of claim 32, wherein R is a hydrocarbon radical having a number average molecular weight of about 700 to about 2,500.
35. The lubricating oil composition of claim 32, wherein R is a hydrocarbon radical having a number average molecular weight of about 710 to about 1,100.
36. The lubricating oil composition of claim 32, wherein R is a polyisobutenyl radical.
37. The lubricating oil composition of claim 32, wherein R is a polyisobutenyl radical having a number average molecular weight of about 700 to about 2,500.
38. The lubricating oil composition of claim 32, wherein R is a polyisobutenyl radical having a number average molecular weight of about 710 to about 1,100.
39. The lubricating oil composition of claim 32, wherein the ethylenically unsaturated carboxylic acid or anhydride is selected from the group consisting of maleic anhydride, citraconic anhydride, itaconic anhydride and mixtures thereof.
40. The lubricating oil composition of claim 32, wherein the acidic molybdenum compound is selected from the group consisting of molybdenum oxide, molybdic acid, ammonium molybdate, sodium molybdate, potassium molybdates, hydrogen sodium molybdate, MoOCl4, MoO2Br2, Mo2O3Cl6, molybdenum trioxide and mixtures thereof.
41. The lubricating oil composition of claim 32, wherein the ethylenically unsaturated carboxylic acid anhydride is maleic anhydride and the acidic molybdenum compound is molybdenum trioxide.
42. The lubricating oil composition of claim 32, wherein the minor amount of the molybdated alkenyl succinimide complex is about 0.001 wt. % to about 10 wt. %, based on the total weight of the composition.
43. The lubricating oil composition of claim 36, wherein the minor amount of the molybdated alkenyl succinimide complex is about 0.001 wt. % to about 10 wt. %, based on the total weight of the composition.
44. The lubricating oil composition of claim 32, further comprising at least one additive selected from the group consisting of metallic detergents, ashless dispersants, friction modifiers, extreme pressure agents, viscosity index improvers and pour point depressants.
45. The lubricating oil composition of claim 32, having a phosphorous content not exceeding 0.05 wt. %, based on the total weight of the composition.
46. The lubricating oil composition of claim 32, having a sulfur content not exceeding 0.4 wt. %, based on the total weight of the composition.
47. A method of operating an internal combustion engine comprising the step of operating the internal combustion engine with the lubricating oil composition of claim 32.
48. A method of operating an internal combustion engine comprising the step of operating the internal combustion engine with the lubricating oil composition of claim 43.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. An isolated polypeptide comprising an amino acid sequence selected from:
a) a polypeptide comprising an amino acid sequence of SEQ ID NO:1,
b) a naturally occurring polypeptide comprising an amino acid sequence at least 90% identical to an amino acid sequence of SEQ ID NO:1,
c) a biologically active fragment of a polypeptide having an amino acid sequence of SEQ ID NO:1, and
d) an immunogenic fragment of a polypeptide having an amino acid sequence of SEQ ID NO:1.
2. An isolated polypeptide of claim 1 having an amino acid sequence of SEQ ID NO:1.
3. A composition comprising the polypeptide of claim 1 and a pharmaceutically acceptable excipient.
4. A composition comprising the polypeptide of claim 2 and a pharmaceutically acceptable excipient.
5. A method for using a polypeptide to screen a plurality of molecules in a sample to identify and purify an agonist, the method comprising:
a) combining the polypeptide of claim 1 with a plurality of molecules under conditions which allow specific binding,
b) detecting agonist activity in the sample; and
c) dissociating the polypeptide from the molecule.
6. An agonist produced by the method of claim 5.
7. A composition comprising the agonist of claim 6 and a pharmaceutically acceptable excipient.
8. A method for using a polypeptide to screen a plurality of molecules in a sample to identify and purify an antagonist, the method comprising:
a) combining the polypeptide of claim 1 with the molecules under conditions which allow specific binding,
b) detecting antagonist activity in the sample; and.
c) dissociating the polypeptide from the molecule.
9. An antagonist produced by the method of claim 8.
10. A composition comprising the antagonist of claim 9 and a pharmaceutically acceptable excipient.
11. A method of using a polypeptide to screen a plurality of compounds to identify a compound which specifically binds polypeptide, the method comprising:
a) combining the polypeptide of claim 1 with the compounds under conditions which allow specific binding, and
b) detecting binding between the polypeptide and a compound, thereby identifying a compound that specifically binds the polypeptide.
12. A method of using a polypeptide to screen a plurality of compounds to identify a compound which modulates the activity of the polypeptide, the method comprising:
a) combining the polypeptide of claim 1 with the compounds under conditions permissive for the activity of the polypeptide,
b) assessing the activity of the polypeptide in the presence of the compound, and
c) comparing the activity of the polypeptide in the presence of the compound with the activity of the polypeptide in the absence of the compound, wherein a change in the activity of the polypeptide in the presence of the compound is indicative of modulation of activity.
13. A compound which modulates the activity of the polypeptide produced by the method of claim 12.
14. A composition comprising the compound of claim 13 and a pharmaceutically acceptable excipient.
15. A method of using a polypeptide to prepare a polyclonal antibody comprising:
a) immunizing an animal with a polypeptide of claim 1 under conditions to elicit an antibody response;
b) isolating antibodies from the animal; and
c) screening the isolated antibodies with the polypeptide, thereby identifying a polyclonal antibody which specifically binds the polypeptide.
16. A polyclonal antibody produced by the method of claim 15.
17. A composition comprising an antibody of claim 16 and an acceptable excipient.
18. A method of using a polypeptide to make a monoclonal antibody, the method comprising:
a) immunizing an animal with a polypeptide of claim 1 under conditions to elicit an antibody response;
b) isolating antibody producing cells from the animal;
c) fusing the antibody producing cells with immortalized cells to form monoclonal antibody-producing hybridoma cells;
d) culturing the hybridoma cells; and
e) isolating from culture the monoclonal antibody which binds specifically to a polypeptide.
19. A monoclonal antibody produced by the method of claim 18.
20. A composition comprising an antibody of claim 19 and an acceptable excipient.
21. A method for producing a polypeptide, the method comprising:
a) culturing a cell under conditions for expression of the polypeptide, wherein the cell is transformed with a recombinant polynucleotide, and the recombinant polynucleotide comprises a promoter sequence operably linked to a polynucleotide encoding the polypeptide of claim 1, and
b) recovering the polypeptide so produced from culture.