1. A high site density glutathione derivatized flow cytometer bead comprising glutathione molecules covalently linked through a crosslinking agent to a polymeric bead, said bead having a diameter ranging from about 1 microns to about 30 microns, said bead having a high site binding density.
2. The derivatized flow cytometer bead according to claim 1 wherein said binding site density of said bead ranges from about 7.5\xd7103 binding sites\u03bcm2 to about 5\xd7105 binding sites\u03bcm2.
3. The derivatized flow cytometer bead according to claim 1 wherein said binding site density of said bead ranges from about 1\xd7104 binding sites\u03bcm2 to about 1\xd7105 binding sites\u03bcm2.
4. The derivatized flow cytomer bead according to claim 1 wherein said binding site density of said bead ranges from about 1\xd7104 binding sites\u03bcm2 to about 5\xd7104 binding sites\u03bcm2.
5. The derivatized flow cytometer bead according to claim 1 wherein said crosslinking agent is linked to said polymeric bead through a hydroxyl group or an amine group on said polymer bead.
6. The derivatized flow cytometer bead according to claim 1 wherein said crosslinking agent has two electrophilic groups or an electrophilic group and a nucleophilic group.
7. The derivatized flow cytometer bead according to claim 6 wherein said crosslinking agent has two electrophilic groups.
8. The derivatized flow cytometer bead according to claim 1 wherein said crosslinking agent is a bis-epoxide crosslinking agent.
9. The derivatived flow cytometer bead according to claim 7 wherein said crosslinking agent is 1,4-butanediyl diglyciyl ether.
10. The derivatized flow cytometer bead according to claim 7 wherein said crosslinking agent is succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (SMCC) or sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC).
11. The derivatized flow cytometer bead according to claim 7 wherein said crosslinking agent is SMCC.
12. The derivatized flow cytometer bead having a diameter of about 3 microns to about 10 microns.
13. The derivatized flow cytometer bead wherein said polymeric bead comprises a polymeric material selected from the group consisting of amino-polystyrene, latex, polycarbohydrate and glass.
14. The derivatized flow cytometer bead according to claim 1 wherein said polymeric bead comprises a crosslinked agarose-dextran composite (Superdex).
15. The derivatized flow cytometer bead according to claim 13 wherein said polymeric material is amino-polystyrene.
16. The derivatized flow cytometer bead according to claim 1 further comprising a GST-fusion protein bound thereto.
17. The derivatized flow cytometer bead according to claim 16 wherein said GST fusion protein comprises GST and a fused protein.
18. The derivatized flow cytometer bead according to claim 17 wherein said fused protein is Bcl-2 protein.
19. The derivatized flow cytometer bead according to claim 17 wherein said fused protein is bound to a binding partner of said protein.
20. The derivatized flow cytometer bead according to claim 17 wherein said binding partner is a protein, peptide, DNA, RNA or a small molecule.
21. The derivatized flow cytometer bead according to claim 17 wherein said fused protein is a Bcl-2 protein.
22. The derivatized flow cytometer bead according to claim 21 wherein said Bcl-2 protein is Bcl-2, Bcl-XL, Bfl-1, Mcl-1, Bcl-W or Bcl-B.
23. The derivatized flow cytometer bead according to claim 19 wherein said fused protein is a Bcl-2 protein.
24. The derivatized flow cytometer bead according to claim 23 wherein said fused protein is Bcl-2, Bcl-XL, Bfl-1, Mcl-1, Bcl-W or Bcl-B.
25. The derivatized flow cytometer bead according to claim 19 wherein said binding partner is fluorescently labeled.
26. The derivatized flow cytometer bead according to claim 25 wherein said binding partner is a fluorescently labeled peptide.
27. The derivatized flow cytometer bead according to claim 26 wherein said peptide is a BH3 peptide based upon sequences found in Bcl-2 proteins.
28. The derivatized flow cytometer bead according to claim 27 wherein said peptide is a Bim BH3 peptide.
29. The derivatized flow cytometer bead according to claim 27 wherein said peptide is a Bid BH3 peptide.
30. The derivatized flow cytometer bead according to claim 27 wherein said peptide is a PUMA BH3 peptide.
31. The derivatized flow cytometer bead according to claim 27 wherein said peptide is a Bak BH3 peptide.
32. The derivatized flow cytometer bead according to claim 19 where said fused protein is a Bcl-2 protein and said binding partner is FITC conjugated epigallocachechin (ECGC).
33. The derivatized flow cytometer bead according to claim 19 wherein said fused protein is a GTPase.
34. The derivatized flow cytometer bead according to claim 33 wherein said GTPase is a Rab, Rac, Rho, Cdc42 or Ras GTPase.
35. The derivatized flow cytometer bead according to claim 34 wherein said binding partner is GTP.
36. The derivatized flow cytometer bead according to claim 34 wherein said GTPase is a Rab GTPase.
37. The derivatized flow cytometer bead according to claim 36 wherein said binding partner is HVps150, hVps34 or a myotubularin.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
I claim:
1. An auxiliary device for moving a payload receptacle of an elevator, comprising: an auxiliary drive device coupleable with a drive unit for the payload receptacle; means for limiting speed of the pay load receptacle; and an image transmission device arranged for sight-free visualization of one of a movable component of the drive unit and a movable component of the speed limiting means, the image transmission device comprising a sensor for detecting images representing movement of one of the drive unit and the speed limiting means, display device for visualization of the images detected by the sensor, and a transfer path connected between the sensor and the display device for transferring the images detected by the sensor to the display device.
2. An auxiliary device according to claim 1, wherein the sensor is a first mirror oriented towards one of the drive unit and the speed limiting means, and the display device is a second mirror, and the second mirror being oriented towards the first mirror so as to form the transfer path.
3. An auxiliary device according to claim 1, wherein the transfer path is a conductor connection.
4. An auxiliary device according to claim 1, wherein the sensor is a camera, the display device is a monitor, and the transfer path is an electric conductor connection.
5. An auxiliary device according to claim 1, wherein the sensor is operative to detect images of the movement of a rotating rotary element connected with the drive unit.
6. An auxiliary device according to claim 5, wherein the rotary element is a shaft of the drive unit, the sensor is a tachometer element arranged at the shaft, and the display device is a tacho display.
7. An auxiliary device according to claim 5, wherein the rotary element is a drive pulley of the drive unit, the pulley having light-permeable zones arranged at mutual angular spacings along a circle arranged concentrically with a rotational axis of the pulley, the image transmission device further including a light source arranged on one side of the rotary element, the sensor being arranged on an opposite side of the rotary element so as to sense light emitted by the light source.
8. An auxiliary device according to claim 7, wherein the light source and the sensor are elements of a light barrier and the transfer path is an electric conductor connection.
9. An auxiliary device according to claim 7, wherein the sensor is an input of an optical conductor arrangement, the conductor connection is the optical conductor arrangement and the display is an output of the optical conductor arrangement.
10. An auxiliary device according to claim 1, and further comprising an emitter and a receiver, the sensor being coupled with the emitter, the display device being coupled with the receiver and the transfer path being one of a radio connection and a laser light connection.
11. A device for monitoring position and movement of a cage in a shaft of an elevator, comprising: first means for monitoring the movement of the cage, which first means includes a unit for detection of the movement, a data transmission path and a display unit; and second means for monitoring the position of the cage, which second means includes means for reproducing the position of the cage.
12. A device according to claim 11, and further comprising a drive unit for movement of the cage, the unit for detection of the movement of the cage being operative to enable a conclusion about the movement of the cage to be made by way of a detection of the movement of a movable part of the drive unit.
13. A device according to claim 11, and further comprising speed-limiting means for limiting cage speed, the unit for detection of the movement of the cage being operative to enable a conclusion about the movement of the cage to be made by way of detection of movement of a movable part of the speed-limiting means.
14. A device according to claim 12, wherein the unit for detection of the movement of the cage comprises a flexible shaft connected with the movable part of the drive unit, the display unit including one of a rotating disc and a tachometer.
15. A device according to claim 13, wherein the unit for detection of the movement of the cage comprises a flexible shaft connected with the movable part of the speed-limiting unit, the display unit including one of a rotating disc and a tachometer.
16. A device according to claim 13, wherein the speed-limiting means includes a cable, the means for reproducing the position of the cage including marks at the cable of the speed-limiting means.
17. A device according to claim 11, and further comprising support means for supporting the cage, the means for reproducing the position of the cage including marks at the support means.