1. A method of measuring HbA1c, wherein the method comprises:
(A) collecting a hemoglobin-containing material from a subject and storing the collected hemoglobin-containing material for a period of at least one day;
(B) reducing carbon dioxide bonded to hemoglobin in the hemoglobin-containing material stored in (A) so as to produce a hemoglobin-containing sample;
(C) cleaving the hemoglobin in the hemoglobin-containing sample produced in (B) by applying a protease treatment to the hemoglobin-containing sample;
(D) treating a glycated part of a hemoglobin fragment obtained from cleaving the hemoglobin in the hemoglobin-containing sample in (C) with fructosyl amine oxidase; and
(E) determining an amount of HbA1c in the hemoglobin-containing sample by measuring a redox reaction between the glycated part and the fructosyl amine oxidase and calculating the amount of HbA1c based on a measurement of the redox reaction.
2. The method of measuring HbA1c according to claim 1, wherein in (B), a strong electrolyte substance having a positive ion and a negative ion is added to the hemoglobin-containing material stored in (A), the positive ion being at least one selected from the group consisting of K+, Na+, and Mg2+, and the negative ion being at least one selected from the group consisting of Cl\u2212, SO42\u2212, and NO3\u2212, wherein in (C), the protease treatment is performed in the presence of the strong electrolyte substance.
3. The method of measuring HbA1c according to claim 2, wherein in (C), the protease treatment is conducted in a reaction solution, and a concentration of the strong electrolyte substance in the reaction solution is 10 to 3000 mmolL.
4. The method of measuring HbA1c according to claim 2, wherein the strong electrolyte substance is at least one selected from the group consisting of KCl, K2SO4, KNO3, NaCl, Na2SO4, NaNO3, MgCl2, MgSO4, and Mg(NO3)2.
5. The method of measuring HbA1c according to claim 1, wherein in (E),
a HbA1c ratio is calculated from the total amount of the hemoglobin in the hemoglobin-containing sample and the amount of HbA1c in the hemoglobin-containing sample.
6. The method of measuring HbA1c according to claim 1, wherein the hemoglobin-containing sample is a whole blood sample or a blood cell sample.
7. A method of measuring HbA1c, wherein the method comprises:
(A) collecting a hemoglobin-containing material from a subject and storing the collected hemoglobin-containing material for a period of at least one day in a state in which carbon dioxide generation is inhibited so as produce a hemoglobin-containing sample;
(B) cleaving hemoglobin in the hemoglobin-containing sample produced in (A) by applying a protease treatment to the hemoglobin-containing sample;
(C) treating a glycated part of a hemoglobin fragment obtained from cleaving the hemoglobin in the hemoglobin-containing sample in (B) with fructosyl amine oxidase; and
(D) determining an amount of HbA1c in the hemoglobin-containing sample by measuring a redox reaction between the glycated part and the fructosyl amine oxidase and calculating the amount of HbA1c based on a measurement of the redox reaction.
8. The method of measuring HbA1c according to claim 7, wherein in (A), the hemoglobin-containing material is stored in the presence of a glycolytic inhibitor, and in (B), the protease treatment is conducted in a reaction solution, and a concentration of the glycolytic inhibitor in the reaction solution is 0.01 to 10 molL.
9. The method of measuring HbA1c according to claim 7, wherein in (D), a HbA1c ratio is calculated from the total amount of the hemoglobin in the hemoglobin-containing sample and the amount of HbA1c in the hemoglobin-containing sample.
10. The method of measuring HbA1c according to claim 7, wherein the hemoglobin-containing sample is a whole blood sample or a blood cell sample.
11. The method of measuring HbA1c according to claim 7, wherein in (A), the hemoglobin-containing material is stored in the presence of a glycolytic inhibitor.
12. The method of measuring HbA1c according to claim 11, wherein the glycolytic inhibitor is at least one selected from the group consisting of sodium fluoride and potassium fluoride.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A digital data transport and storage system between a file in a personal device and a file destination comprising:
A digital data file in said personal device;
A preprocessor is configured to perform a transformation from multiple inputs to multiple outputs;
wherein the multiple inputs comprising a first input stream for said digital data file, and a second stream of said digital file as a digital envelop;
wherein a first output of the preprocessor comprising an enveloped digital data, and
A transmission channel is configured to connect between said first output of the preprocessor and said destination.
2. The digital data file transport and storage system of claim 1 wherein said enveloped digital data further comprising a weighted sum of the first and the second inputs and the weighted sum in a digital format appearing to human audio sensors with substantially identical audio features as said second input stream.
3. The digital data file transport and storage system of claim 1 wherein said digital envelop is known to all intended reading devices.
4. The digital data file transport and storage system of claim 1, is configured on a portable personnel device or a PC.
5. The digital data file transport and storage system of claim 1, is configured between a portable personnel device or a PC and a distributed storage system.
6. The storage system of claim 5, comprising a storage on cloud.
7. The digital data file transport and storage system of claim 1, wherein said pre-processor is further configured to select an enveloped digital file for display.
8. The digital data file transport and storage system of claim 1, wherein said transform in said pre-processor further comprising a preferential weighting to one of said inputs in generating multiple digital outputs in image, video, or audio formats to human sensors with substantially identical appearances to a format of appearance of said input with the preferential weighting.
9. The digital data file transport and storage system of claim 1, wherein said transform in said pre-processor further comprising a wavefront multiplexing with an orthogonal matrix transform,
10. The transform in of claim 9 further comprising a Fourier transform.
11. The transform in of claim 9 further comprising a Hadamard transform.
12. The digital data file transport and storage system of claim 1, wherein said transform in said pre-processor further comprising a wavefront multiplexing with a non-orthogonal full-rank matrix transform.
13. The digital data file transport and storage system of claim 1, wherein the said multiple inputs to said pre-processor are further configured to connect to a common known data set but with known different delays for various inputs.
14. The digital data file transport and storage system of claim 1, wherein one of said multiple outputs to said pre-processor is grounded.
15. The digital data file transport and storage system of claim 1, wherein one of said multiple outputs to said pre-processor is grounded.
16. The digital data file transport and storage system of claim 1, wherein said multiple inputs to said pre-processor further comprises an authentication data set.
17. A digital data file retrieval and transport system between a file cloud storage and a file destination in a receiving site, comprising
A stored digital file in a form of enveloped digital data in said file cloud storage;
A postprocessor in said receiving site is configured to perform a transformation from multiple inputs to multiple outputs;
wherein the multiple inputs comprising a first input stream for said stored enveloped digital data file;
wherein a first output of the postprocessor comprising a reconstituted digital data file,
A transmission channel configured to connect said first input of the postprocessor and said cloud storage;
18. The digital data file retrieval and transport system of claim 17, wherein said reconstituted digital data further comprising a weighted sum of the first and the second inputs and wherein said first input in a digital format appearing to human sensors with substantially identical audio features as said second input stream.
19. The digital data file retrieval and transport system of claim 17, wherein the multiple inputs further comprising a second stream of a digital file as a digital envelop file.
20. The digital data file retrieval and transport and storage system of claim 14, is configured on a portable personnel device or on a PC.