What is claimed is:
1. A fluid processing method of an object to be processed characterized by the front and rear surfaces of said object to be processed being covered by a front shielding plate and a rear shielding plate in order to prevent the front and rear surface of said object to be processed from becoming contaminated and said object to be processed being allowed to rotate relative to said front shielding plate and rear shielding plate,
fluid being supplied between the front surface of said object to be processed and said front shielding plate and a fluid process of the front surface of said object to be processed being carried out.
fluid being collected after the fluid processing of the front surface of said object to be processed completes and,
this liquid collected from the completed fluid processing of the front surface of the object to be processed being supplied between the rear surface of said object to be processed and said rear shielding plate and thereafter fluid processing of the rear surface of said object to be processed being carried out.
2. A fluid processing method of an object to be processed as set forth in claim 1 characterized by,
fluid being collected after the fluid processing of the rear surface of said object to be processed completes and,
this liquid collected from the liquid that completed fluid processing of the rear surface of the object to be processed being supplied between the rear surface of said object to be processed and said rear shielding plate and thereafter fluid processing of the rear surface of said object to be processed being carried out.
3. A fluid processing method of an object to be processed as set forth in claim 1 characterized by a hood used for fluid capture being arranged on the periphery of said object to be processed and designed to rotate on the same axis as said object to be processed or said front shielding plate and said rear shielding plate and,
said hood rotating along with said object to be processed or said front shielding plate and said rear shielding plate and a centrifugal force being applied to said fluid from the completed fluid processing in order to collect said fluid from the completed fluid processing, and because of this only fluid from the completed fluid processing of said object to be processed discharged outward radially in captured by said hood.
4. A fluid processing method of an object to be processed as set forth in claim 2 characterized by fluid that completed fluid processing of the front surface of said object to be processed being mixed with fluid that completed fluid processing of the rear surface of said object to be processed and then being collected.
5. A fluid processing method of an object to be processed an set forth in claim 1 characterized by fluid to be supplied being selected in proportion to fluid processing that should be carried out for said object to be processed and thereafter fluid processing of the front and rear surfaces of said object to be processed being carried out.
6. A fluid processing apparatus of an object to be processed comprising:
a retention member for the object to be processed that retains the object to be processed,
front and rear shielding plates which shield both the front surface and the rear surface of said object to be processed retained by said retention member for the object to be processed and rotate relative to said retention member for the object to be processed,
a fluid supply system that supplies fluid between said front shielding plate and the front surface of said object to be processed through a supply opening provided on said front shielding plate,
a hood that captures liquid that at least completed fluid processing of the front surface of the object to be processed,
a collection portion that collects said fluid that completed fluid processing captured by said hood from an outlet opening provided on said hood,
a circulation system that supplies said fluid that completed fluid processing collected by means of said collection portion between said rear shielding plate and the rear surface of the object to be processed through a supply opening provided on said rear shielding plate in order to carry out fluid processing on said rear surface of the object to be processed and then collects the fluid that completed fluid processing of said rear surface of the object to be processed.
7. A fluid processing apparatus of an object to be processed as set forth in claim 6 comprised such that said hood is arranged to rotate on the same axis as said retention member for the object to be processed or said front shielding plate and said rear shielding plate, and a centrifugal force generated by said rotation is received capturing the fluid that completed fluid processing of the front surface of the object to be processed and the fluid that completed fluid processing of the front surface of the object to be processed both discharged from the periphery of said object to be processed.
8. A fluid processing apparatus of an object to be processed as set forth in claim 6 wherein said retention member for the object to be processed is allowed to rotate and said front shielding plate and said rear shielding plate are fixed.
9. A fluid processing apparatus of an object to be processed as set forth in claim 6 wherein said retention member for the object to be processed is comprised by a hollow rotating axle provided on said rear shielding plate wherein a supply opening is provided, a retention portion provided on the periphery of said object to be processed that retains the periphery of said object to be processed, and a linkage portion that protrudes from said rotating axle outward radially reaching close to the periphery of said rear shielding plate and that links said retention portion to said rotating axle.
10. A fluid processing apparatus of an object to be processed as set forth in claim 6 wherein said hood is comprised by an external hood that covers the front side of the travel path of the fluid that completed processing discharged from the periphery of the object to be processed and an internal hood that covers the lower portion of the travel path of the fluid that completed processing and in addition at least said internal hood rotates on the same axis as said retention member for the object to be processed.
11. A fluid processing apparatus of an object to be processed as set forth in claim 6 wherein said retention member for the object to be processed and said hood are comprised integrally.
12. A fluid processing apparatus of an object to be processed an set forth in claim 6 wherein said retention member for the object to be processed and said hood are formed separate.
13. A fluid processing apparatus of an object to be processed as set forth in claim 6 characterized by a mechanism being provided that regulates the circulation flowrate of fluid in said circulation system.
14. A fluid processing apparatus of an object to be processed as set forth in claim 13 characterized by said mechanism that regulates the circulation flowrate of fluid in said circulation system being a cyclone separator that utilizes the centrifugal force of fluid.
15. A fluid processing apparatus of an object to be processed as set forth in claim 6 characterized by said front shielding plate and rear shielding plate being formed by carbon or fluoride resin.
16. A fluid processing apparatus of an object to be processed as set forth in claim 6 characterized by said collection portion being fixed and a lower portion outlet opening of said hood being inserted into this fixed collection portion without making contact.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An aminated polyamine having the formula:
or its possible stereoisomers or a salt thereof with a pharmaceutically acceptable acid wherein:
R1 and R4 may be the same or different and are alkyl, aryl, aryl alkyl or cycloalkyl, optionally having an alkyl chain interrupted by at least one etheric oxygen atom;
R2 and R3 may be the same or different and are R1, R4 or H;
N1, N2, N3 and N4 are nitrogen atoms capable of protonation at physiological pH’s;
ALK1, ALK2 AND ALK3 may be the same or different and are straight or branched chain alkylene bridging groups having 1 to 4 carbon atoms which effectively maintain the distance between the nitrogen atoms such that the polyamine:
(i) is capable of uptake by a target cell upon administration of the polyamine to a human or non-human animal or is capable of binding to at least one polyamine site of a receptor located within or on the surface of a cell upon administration of the polyamine to a human or non-human animal; and
(ii) upon uptake by the target cell, competitively binds via an electrostatic interaction between the positively charged nitrogen atoms to biological counter-anions;
\u2003the polyamine, upon binding to the biological counter-anion in the cell, functions in a manner biologically different than the intracellular polyamines; and
\u2003further wherein at least one of said bridging groups ALK1, ALK2 and ALK3 contains at least one \u2014CH(NRH)\u2014 group which is not alpha- to either of the nitrogen atoms and R is H, alkyl, acyl or sulfonyl.
2. A polyamine of claim 1 having the formula:
3. A polyamine of claim 1 having the formula:
4. A pharmaceutical composition comprising an antineoplastic effective amount of an aminated polyamine of claim 1 or a salt thereof with a pharmaceutically acceptable acid and a pharmaceutically acceptable carrier therefore.
5. A method of treating a neoplasm in a human or non-human animal in need thereof comprising administering thereto an anti-neoplastic effective amount of an aminated polyamine of claim 1 or a salt thereof with a pharmaceutically acceptable acid.
6. An article of manufacture comprising packaging material and a pharmaceutical agent contained within said packaging material, wherein said pharmaceutical agent is effective for the treatment of a subject requiring antineoplastic therapy, and wherein said packaging material comprises a label which indicates that said pharmaceutical agent can be used for antineoplastic therapy, and wherein said pharmaceutical agent is an aminated polyamine of claim 1.
7. A method of increasing uptake of a pharmacore into a cell, comprising covalently attaching a polyamine to the pharmacore, optionally indirectly via a linking group, to form a conjugate having a polyamine moiety, wherein the polyamine moiety comprises the aminated polyamine of claim 1, and contacting the cell with the conjugate or a salt, solvate or hydrate thereof.
8. The method of claim 7 wherein said pharmacore is an antibiotic, antiviral or chelating agent.
9. A vector adapted for the intracellular delivery of a pharmacore comprising a conjugate of a polyamine covalently attached, optionally indirectly attached via a linking group, to a pharmacore, said conjugate having a polyamine moiety, wherein the polyamine moiety comprises the aminated polyamine of claim 1.
10. The Vector of claim 9 wherein said pharmacore is an antibiotic, antiviral or chelating agent.