1. A test device for colored particle immunoassay in which an extracted liquid sample is caused to pass through a colorimetric monoclonal antibody which is fixed to micro gold and a C site and a T site to which two types of multiple monoclonal antibodies are attached, so that the liquid sample specifically reacts with an antibody and produces a color at the C site,
the test device comprising a transparent tubular body having a through-hole, the C site and the T site being attached to an inside wall of the through-hole, a receiving piece connected to a front end of the through-hole in a front portion of the transparent tubular body, and a pressure cap positioned in front of the transparent tubular body and coupled to the receiving piece.
2. The test device according to claim 1, wherein the colorimetric monoclonal antibody bonded to the micro gold is attached to an upper surface of the receiving piece.
3. The test device according to claim 1, wherein the colorimetric monoclonal antibody bonded to the micro gold is attached to the inside wall of the front end of the through-hole of the transparent tubular body.
4. The test device according to claim 1, wherein the receiving piece has a recessed portion.
5. The test device according to claim 1, wherein the receiving piece has guide walls on both sides thereof.
6. The test device according to claim 1, wherein a residue absorbent material is disposed in a rear end of the through-hole of the transparent tubular body.
7. The test device according to claim 6, wherein a discharge hole is formed behind the residue absorbent material which is disposed in the rear end of the transparent tubular body.
8. The test device according to claim 1, wherein an O-ring is disposed on an inner circumference of the pressure cap.
9. The test device according to claim 1, wherein a pressure-reducing hole with which pressure is to be reduced is formed in a portion of the pressure cap.
10. The test device according to claim 1, wherein a handle which is coupled to a rear end of the transparent tubular body is provided.
11. The test device according to claim 10, wherein the handle which is coupled to the rear end of the transparent tubular body receives therein the residue absorbent material.
12. The test device according to claim 11, wherein the handle which receives therein the residue absorbent material in the rear portion of the transparent tubular body has a discharge hole.
13. The test device according to claim 1, wherein the multiple monoclonal antibodies of the C site and the T site attached to an interior of the transparent tubular body are attached to upper and lower portions inside the through-hole and spaced apart from each other by a predetermined distance.
14. (canceled)
15. The test method according to claim 18, further comprising:
a pressure reducing step of, when a pressure applied to the transparent tubular body by the pressure cap is excessively high, reducing the pressure via a pressure-reducing hole formed in one portion of the pressure cap.
16. The test method according to claim 15, further comprising: a discharge step of discharging the pressure through the discharge hole,
said discharge step being performed after the reaction step;
said discharge step being performed in addition to the residue absorption.
17. A method of preparing a test device for colored particle immunoassay, comprising:
a molding step of molding a transparent tubular member having a through-hole;
a receiving piece-forming step of providing a receiving piece in a portion of the transparent tubular member;
a step of arranging a colorimetric monoclonal antibody in which micro gold having a monoclonal antibody fixed thereto is arranged on the receiving piece or in the through-hole in a front end of the transparent tubular member; and
an antibody attachment step of attaching two types of multiple monoclonal antibodies having a shape of a belt to a C site and a T site which are allotted to the through-hole inside the transparent tubular body, thereby preparing said test device.
18. A test method for colored particle immunoassay using the test device prepared by the method of claim 17, comprising:
a reaction step of placing an extracted liquid sample on the receiving piece and, under a positive pressure that is produced by a pressure cap which is fitted into a portion of the receiving piece, allowing the liquid sample that has reacted with the colorimetric monoclonal antibody of the micro gold on the receiving piece to flow into the through-hole inside the transparent tubular body, be condensed and produce a color at the C site, and arrive at the T site, so that whether or not a specific antigen is present in the liquid sample is determined; and
after the reaction step, a residue absorption step of allowing the liquid sample that is discharged through the rear end of the through-hole of the transparent tubular body to be absorbed to an absorbent material disposed in the rear end of the through-hole.
19. A test method for colored particle immunoassay using the test device of claim 1, comprising:
a reaction step of placing an extracted liquid sample on the receiving piece and, under a positive pressure that is produced by the pressure cap, allowing the liquid sample that has reacted with the colorimetric monoclonal antibody of the micro gold to flow into the through-hole inside the transparent tubular body, be condensed and produce a color at the C site, and arrive at the T site, so that whether or not a specific antigen is present in the liquid sample is determined; and
after the reaction step, a residue absorption step of allowing the liquid sample to be discharged through the rear end of the through-hole of the transparent tubular body and absorbed to an absorbent material disposed in the rear end of the through-hole.
20. A test method for colored particle immunoassay using a test device comprising:
a transparent tubular member having a through-hole;
a receiving piece in a portion of the transparent tubular member;
micro gold having a monoclonal antibody fixed thereto arranged on the receiving piece or in the through-hole in a front end of the transparent tubular member; and
two types of multiple monoclonal antibodies having a shape of a belt to a C site and a T site which are positioned in the through-hole inside the transparent tubular body;
wherein said test method comprises:
a reaction step of placing an extracted liquid sample on the receiving piece and, under a positive pressure that is produced by a pressure cap which is fitted into a portion of the receiving piece, allowing the liquid sample that has reacted with the colorimetric monoclonal antibody of the micro gold on the receiving piece to flow into the through-hole inside the transparent tubular body, be condensed and produce a color at the C site, and arrive at the T site, so that whether or not a specific antigen is present in the liquid sample is determined; and
after the reaction step, a residue absorption step of allowing the liquid sample that is discharged through the rear end of the through-hole of the transparent tubular body to be absorbed to an absorbent material disposed in the rear end of the through-hole.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A coupling device for connecting a first and a second crankshaft each having a plurality of pistons drivingly connected thereto, said coupling device comprising:
a first clutch member drivingly attached to the first crankshaft;
a second clutch member attached to the second crankshaft;
an actuation mechanism movable between a first position for frictionally engaging said first clutch member with said second clutch member and a second position for disengaging said first clutch member from said second clutch member; and
a phase clutch mechanism including a first clutch portion mounted to the first crankshaft and a second clutch portion mounted to the second crankshaft, said first and second clutch portions being engageable only when said second crankshaft is at a predetermined phase angle relative to said first crankshaft.
2. The coupling device of claim 1, wherein said actuation mechanism includes a gimbal assembly for applying a force against a pressure plate which acts against said clutch disc.
3. The coupling device of claim 1, wherein said actuation mechanism includes a drive motor for driving a gear member, said gear member drivingly engaging a gear segment of an actuation lever.
4. The coupling device of claim 3, wherein said drive motor includes a servo brake for holding an output shaft of said drive motor in a predetermined position.
5. The coupling device of claim 3, wherein said actuation mechanism includes a detent mechanism for engaging said actuation lever in an engaged position.
6. The coupling device of claim 1, wherein said phase clutch device is an angular-specific dog clutch.
7. The coupling device of claim 1, wherein one of said first and second clutch portions of said phase clutch device includes a hub portion with a recessed roller groove in a surface thereof and the other of said first and second clutch portions of said phase clutch device includes a roller engageable with said hub portion for locking receipt in said recessed roller groove when said first and second crankshafts are at a predetermined phase angle position relative to one another.
8. The coupling device of claim 1, wherein said phase clutch device includes an overrunning clutch for permitting the first crankshaft to rotate relative to the second crankshaft.
9. A power unit, comprising:
a first crankshaft having a plurality of pistons drivingly connected thereto;
a second crankshaft having a plurality of pistons drivingly connected thereto; and
a phase clutch mechanism for connecting said first and said second crankshafts, said phase clutch mechanism including a first clutch portion mounted to said first crankshaft and a second clutch portion mounted to said second crankshaft, said first and second clutch portions being engageable only when said second crankshaft is at a predetermined phase angle relative to said first crankshaft.
10. The power unit of claim 9, wherein said phase clutch device is an angular-specific dog clutch.
11. The power unit of claim 9, wherein one of said first and second clutch portions of said phase clutch device includes a hub portion with a recessed roller groove in a surface thereof and the other of said first and second clutch portions of said phase clutch device includes a roller engageable with said hub portion for locking receipt in said recessed roller groove when said first and second crankshafts are at a predetermined phase angle position relative to one another.
12. The power unit of claim 9, further comprising a clutch disc drivingly attached to one of said first and second crankshafts, a clutch plate attached to the other of said first and second crankshafts, and an actuation mechanism movable between a first position for engaging said clutch disc with said clutch plate and a second position for disengaging said clutch disc from said clutch plate.
13. The power unit of claim 12, wherein said actuation mechanism includes a gimbal assembly for applying a force against a pressure plate which acts against said clutch disc.
14. The power unit of claim 12, wherein said actuation mechanism includes a drive motor for driving a gear member, said gear member drivingly engaging a gear segment of an actuation lever.
15. The power unit of claim 14, wherein said drive motor includes a servo brake for holding an output shaft of said drive motor in a predetermined position.
16. The power unit of claim 14, wherein said actuation mechanism includes a detent mechanism for engaging said actuation lever in an engaged position.
17. The power unit of claim 9, wherein said phase clutch device includes an overrunning clutch for permitting said first crankshaft to rotate relative to said second crankshaft.
18. A power unit for a vehicle, comprising:
a first engine portion including a first crankshaft having a plurality of pistons drivingly connected thereto;
a second engine portion including a second crankshaft having a plurality of pistons drivingly connected thereto; and
a starter clutch mechanism operable to engage said first and said second crankshafts to drive said second crankshaft to start said second engine portion, said starter clutch mechanism including an actuator mechanism for actuating said starter clutch mechanism; and
an engine control unit for monitoring operating conditions of the vehicle for determining a required engine torque output wherein when said required engine torque output exceeds a predetermined level, said engine control unit provides a signal to said actuator mechanism to actuate said starter clutch mechanism to start said second engine portion.
19. The power unit of claim 18, wherein said engine control unit controls operation of said second engine portion to synchronize a speed of said second engine portion with said first engine portion.
20. The power unit of claim 19, further comprising a phase clutch mechanism for connecting said first and said second crankshafts, said phase clutch mechanism including a first clutch portion mounted to said first crankshaft and a second clutch portion mounted to said second crankshaft, said first and second clutch portions being engageable only when said second crankshaft is at a predetermined phase angle relative to said first crankshaft.
21. The power unit of claim 20, wherein said phase clutch device is an angular-specific dog clutch.
22. The power unit of claim 20, wherein one of said first and second clutch portions of said phase clutch device includes a hub portion with a recessed roller groove in a surface thereof and the other of said first and second clutch portions of said phase clutch device includes a roller engageable with said hub portion for locking receipt in said recessed roller groove when said first and second crankshafts are at a predetermined phase angle position relative to one another.
23. The power unit of claim 20, wherein said phase clutch device includes an overrunning clutch for permitting said first crankshaft to rotate relative to said second crankshaft.