1. A composition for therapeutic use as a wound cover, comprising (i) epithelial cells derived from allogenic or autologous epidermis and (ii) a biopolymer membrane polymer selected from the group consisting of polylactic acid (PLA), polyglycolic acid (PGA) and polylactic-polyglycolic acid copolymer (PLGA).
2. The composition of claim 1, wherein the epithelial cells are present as a monolayer on the biopolymer membrane.
3. The composition of claim 2, wherein said monolayer is a sub-confluent monolayer.
4. The composition of claim 3, wherein said monolayer is at least 20% confluent.
5. The composition of claim 4, wherein said monolayer is at least 50% confluent.
6. The composition of claim 1, wherein the epithelial cells are keratinocyte cells.
7. The composition of claim 6, wherein the keratinocyte cells are keratinocyte stem cells.
8. The composition of claim 1, wherein the epithelial cells are transient amplifying cells.
9. The composition of claim 6, wherein the keratinocyte cells are present on the biopolymer membrane in a sub-confluent monolayer.
10. The composition of claim 6, wherein the keratinocyte cells comprise cells capable of further proliferation.
11. The composition of claim 6, wherein the keratinocyte cells comprise undifferentiated cells.
12. The composition of claim 1, wherein at least 90% of the epithelial cells are actively proliferating keratinocytes.
13. The composition of claim 1, wherein said cells retain substantial viability on the wound cover for at least 72 hours after application to a wound.
14. The composition of claim 1 wherein said biopolymer membrane is selected from biodegradable, biocompatible natural or synthetic material.
15. The composition of claim 1 wherein the biopolymer membrane selected from the group consisting of polylactic acid (PLA), polyglycolic acid (PGA) and polylactic-polyglycolic acid copolymer (PLGA) has a molecular weight of at least 50,000 Da.
16. A process for preparing the composition of claim 1 comprising the steps of:
a) isolating keratinocyte cells from allogenic or autologous epidermis;
b) preparing a cell suspension comprising the isolated keratinocyte cells;
c) expanding the keratinocyte cells;
d) optionally, cryopreserving the keratinocyte cells;
e) optionally, thawing the keratinocyte cells;
f) seeding the keratinocyte cells onto a biopolymer membrane;
g) expanding the keratinocyte cells into a sub-confluent monolayer on the biopolymer membrane; and
h) transporting the biopolymer membrane using a transport device comprising transport media.
17. The process of claim 16, wherein the keratinocytes cells are seeded onto biopolymer membrane sheets comprising PLA, at a cell concentration of 0.10\xd7106 to 0.5\xd7106cm.2.
18. The process of claim 16, wherein the wound cover is transferred to a transport device made of polycarbonate.
19. The process of claim 16, wherein the transport media comprises carbon dioxide enriched media.
20. A method of treating a wound in an individual comprising application of the composition of claim 1 to said wound in the individual, whereby said application is effective for the treatment of such wound.
21. The method of claim 20, wherein said wound is selected from the group consisting of:
a) a post-operative wound;
b) a burn;
c) a diabetic ulcer;
d) a bed sore; and
e) a traumatic injury.
22. The method of claim 20, wherein said individual is human.
23. A composition comprising a wound cover comprising a sub-confluent monolayer of keratinocyte cells and a biopolymer membrane.
24. A composition comprising a wound cover comprising a monolayer of undifferentiated keratinocyte cells on a biopolymer membrane.
25. A wound cover composition comprising (1) a sub-confluent monolayer of keratinocyte cells comprising undifferentiated cells and (2) a biopolymer membrane, wherein the monolayer is on the biopolymer membrane.
26. A method for treating a wound in a subject, comprising using a biopolymer membrane as a delivery system, and delivering to the wound a sub-confluent monolayer of keratinocyte cells comprising undifferentiated cells.
27. A kit for treating a wound, wherein the kit comprises (1) a wound cover comprising (a) a sub-confluent monolayer of keratinocyte cells comprising undifferentiated cells and (b) a biopolymer membrane, wherein the monolayer is on the biopolymer membrane; and (2) a transport device.
28. A composition comprising a wound cover comprising a monolayer of proliferating basal epithelial cells on a biopolymer membrane.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for controlling read data buffering, comprising:
performing core operations in response to a receipt of a read command from a master controller;
determining which of either an internal or external communication buffer of one data storage node of one or more data storage nodes that is to forward information to said master controller, wherein said determining is based upon constraints and contents of one or more communication buffers of said one or more storage nodes; and
forwarding information from said internal or external communication buffer to said master controller.
2. The method of claim 1 wherein said forwarding information from said internal or external communication buffer comprises placing a burst of data from a data storage node located further away from said master controller than a data storage node located nearer said master controller between bursts of data sent to said master controller from said data storage node located nearer said master controller.
3. The method of claim 1 wherein said determining comprises determining if data exists in an internal communication buffer of a data storage node located nearest said master controller, and forwarding data to said master controller if it is determined that data exists in said internal communication buffer of said data storage node located nearest said master controller.
4. The method of claim 1 wherein said determining comprises determining if data is ready to be sent to said master controller by an internal read of a data storage node located nearest said master controller, and storing this data in an internal read buffer of said data storage node located nearest said master controller at the next available entry.
5. The method of claim 4 wherein said determining comprises determining if a data storage node located further away from said master controller than said data storage node that is located nearest said master controller is sending data to said data storage node located nearest said master controller, and storing this data in an external communication buffer of said data storage node located nearest said master controller at the next available entry, if it is determined that said data storage node located further away from said master controller than said data storage node located nearest said master controller is sending data to said data storage node located nearest said master controller.
6. The method of claim 1 wherein said determining comprises determining if data exists in an external communication buffer of a data storage node located nearest said master controller, and sending data to said master controller in a first in first out (FIFO) manner from said external communication buffer if it is determined that data exists in said external communication buffer of said data storage node located nearest said master controller.
7. The method of claim 1 wherein said determining is based on an algorithm that selects external traffic over internal traffic in a static decision and prioritizes data from further data storage nodes over data from data storage nodes that are nearer.
8. A data storage system that includes data buffering control, comprising:
one or more data storage nodes for storing information, wherein each of said one or more data storage nodes includes one or more communication buffers; and
a master controller coupled to said one or more data storage nodes for controlling a flow of information from said one or more data storage nodes to said master controller based upon constraints and contents of one or more communication buffers of a data storage node that is coupled to said master controller, wherein based on said constraints and said contents, a communication buffer of one of said one or more data storage nodes is selected to send information to said master controller.
9. The storage system of claim 8 wherein said controlling said flow of information from said one or more data storage nodes to said master controller comprises placing a burst of data from a data storage node located further away from said master controller than a data storage node located nearest said master controller between bursts of data sent to said controller from said data storage node that is located nearest to said master controller.
10. The storage system of claim 8 wherein said controlling said flow of information from said one or more data storage nodes comprises adjusting the timing of an issuance of a read command to be executed earlier or later based upon a determined latency.
11. The storage system of claim 8 wherein said controlling said flow of information from said one or more data storage nodes comprises determining if data exists in an internal communication buffer of a data storage node that is coupled nearest to said master controller, and sending data to said master controller if it is determined that data exists in said internal communication buffer of said storage node nearest to said master controller.
12. The storage system of claim 11 wherein said controlling said flow of information from said one or more data storage nodes comprises determining if data is ready to be sent to said master controller by an internal read of a data storage node that is coupled nearest to said master controller, and storing this data in an internal communication buffer of said data storage node coupled nearest to said master controller at the next available entry.
13. The storage system of claim 12 wherein said controlling said flow of information from said one or more data storage nodes comprises determining if a data storage node that is coupled further from said master controller is sending data to said data storage node that is coupled nearest to said master controller, and storing this data in an external communication buffer of said data storage node that is coupled nearest to said master controller at the next available entry, if it is determined that said data storage node that is coupled further from said master controller is sending data to said data storage node that is coupled nearest to said master controller.
14. The method of claim 8 wherein said controlling said flow of information from said one or more data storage nodes comprises determining if data exists in an external communication buffer of said data storage node that is coupled nearest to said master controller, and sending data to said master controller in a first in first out (FIFO) manner if it is determined that data exists in said external communication buffer of said data storage node coupled nearest to said master controller.
15. The method of claim 8 wherein said controlling said flow of information is based on an algorithm that selects external data traffic over internal data traffic in a static decision and prioritizes data from data storage nodes located further from said master controller over data from data storage nodes that are located nearer said master controller.
16. A computer readable medium having computer-executable components comprising:
a read scheduling component for scheduling the timing of a read command sent from a master controller to one of one or more data storage nodes;
a read command communicating component for communicating a read command to said one of said one or more data storage nodes;
a read data buffer selection component for determining an internal or external communication buffer of one of said one or more data storage nodes that is to send information to said controller based upon constraints and contents of one or more communication buffers of one or more of said one or more data storage nodes; and
a data forwarding component for forwarding information from said internal or external communication buffer to said master controller.
17. The medium of claim 16 wherein said sending information from said internal or external communication buffer comprises placing a burst of data forwarded from a data storage node located further away from said master controller than a data storage node located nearer said master controller between bursts of data sent to said master controller from said data storage node located nearer said master controller.
18. The medium of claim 16 wherein said scheduling said timing of said read command comprises adjusting a read command to be executed earlier or later based upon a determined latency.
19. The method of claim 16 wherein said determining comprises determining if data exists in an internal communication buffer of a data storage node located nearest said master controller, and forwarding data to said controller if it is determined that data exists in said internal communication buffer of said node located nearest said master controller.
20. The method of claim 16 wherein said determining is based on an algorithm that selects external traffic over internal traffic in a static decision and prioritizes data from further data storage nodes over data from data storage nodes that are nearer.