1. A method for purifying a multiunit complex comprising a first tryptophanyl tRNA synthetase fragment and a second tryptophanyl tRNA synthetase fragment, wherein the purified complex comprises less than 10 endotoxin units per milligram of synthetase fragment, and wherein the method comprises performing an endotoxin-reduction filtration step after performing a clarification step and prior to performing at least one of the steps selected from: a buffer exchange; a concentration step; and a cation-exchange chromatographic step, wherein said endotoxin-reduction filtration step does not include the use of a detergent.
2. A method for purifying a multiunit complex comprising a first tryptophanyl tRNA synthetase fragment and a second tryptophanyl tRNA synthetase fragment, wherein the purified complex comprises less than 10 endotoxin units per milligram of synthetase fragment, and wherein the method comprises performing an endotoxin-reduction filtration step after performing an anion-exchange chromatographic step, wherein said endotoxin-reduction filtration step does not include the use of a detergent.
3. The method according to claim 1, wherein said first tRNA synthetase fragment and said second tRNA synthetase fragments are identical.
4. The method according to claim 3, wherein said first tRNA synthetase fragment consists of SEQ ID NO: 27.
5. The method according to claim 1, wherein said first tRNA synthetase fragment consists of SEQ ID NO: 27 and said second tRNA synthetase fragment consists of SEQ ID NO: 24 or SEQ ID NO: 27.
6. The method according to claim 1, wherein said first tRNA synthetase fragment and said second tRNA synthetase fragment are each independently selected from the group consisting of SEQ ID Nos. 24, SEQ ID No. 25, SEQ ID No. 26, SEQ ID No. 27, SEQ ID No. 28, SEQ ID No. 29, SEQ ID No. 36, SEQ ID No. 37, SEQ ID No. 38, SEQ ID No. 39, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No. 48, SEQ ID No. 49, SEQ ID No. 50, SEQ ID No. 51, SEQ ID No. 52 and SEQ ID No. 53, or homologs thereof, and wherein said first and second fragments are covalently dimerized.
7. The method according to claim 1, wherein said multiunit complex has greater than 50 angiostatin activity unites; and at least 99% level of purity.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A conveyor system including:
a rotatably-mounted drum having a bottom surface;
a conveyor belt including a conveying surface that is movable along a path that includes a helical portion around the drum such that, during movement of the conveyor belt, a surface of the drum frictionally engages an inside edge of the conveyor belt along the helical portion of the path; and
a rotating driving element, a peripheral surface of the rotating driving element to directly engage the drum and to rotate the drum thereby operatively to move the conveyor belt along the path, the rotating driving element including a roller defining the peripheral surface to directly engage the bottom surface of the drum with a frictional engagement to rotate the drum.
2. The system of claim 1, wherein the roller is vulcanized with polyurethane to increase the frictional engagement between the roller and the drum.
3. The system of claim 1, wherein the drum is shaftless.
4. The system of claim 1, wherein the roller is tapered to maintain the same velocity at a plurality of contact points between the peripheral surface of the roller and the bottom surface of the drum.
5. The system of claim 1, wherein the roller is positioned under the drum to hold the drum in a vertical position and reduce horizontal load on an external frame that supports the belt.
6. A conveyor system including:
a rotatably-mounted drum without a center shaft;
a conveyor belt including a conveying surface that is movable in a path that includes a helical portion around the drum such that, during movement of the conveyor belt a surface of the drum frictionally engages an inside edge of the conveyor belt along the helical portion of the path; and
a rotating driving element to directly engage the drum and to rotate the drum, thereby operatively to move the conveyor belt along the path, the rotating driving element including a roller to directly engage the drum with a frictional engagement to rotate the drum.
7. The system of claim 6, wherein the drum includes at least one of an outside bottom surface, an outside top surface, and an outside cylinder surface and the rotating driving element directly engages at least one of the outside bottom surface, the outside top surface, and the outside cylinder surface.
8. The system of claim 6, wherein the rotating driving element includes a peripheral surface that directly engages the drum.