1. A method for stabilizing connective tissue in a living subject, the method comprising applying an effective amount of a composition directly to the connective tissue in vivo to stabilize the tissue, wherein the composition has a pH between about 4 and about 9, the connective tissue comprises elastin and the composition comprises a compound that interacts with the elastin in the connective tissue to stabilize the connective tissue.
2. The method of claim 1 wherein the connective tissue is within a blood vessel.
3. The method of claim 1 wherein the compound is a phenolic compound.
4. The method of claim 1 wherein the compound has a hydrophobic region and a plurality of functional groups capable of hydrogen bonding.
5. The method of claim 1 wherein the compound is a flavonoid or a flavonoid derivative, a flavolignan or a flavolignan derivative, a phenolic rhizome or a phenolic rhizome derivative, a flavan-3-ol or a flavan-3-ol derivative, a tannin or a tannin derivative, an ellagic acid or an ellagic acid derivative, a procyanidin or a procyanidin derivative, a combination thereof, or a pharmaceutically acceptable salt thereof.
6. The method of claim 1 wherein the compound is anthocyanins, quercetin, (+)-catechin, (\u2212)-epicatechin, tannic acid, pentagalloylglucose, nobotanin, epigallocatechin gallate, gallotannins, a combination thereof, or a pharmaceutically acceptable salt thereof.
7. The method of claim 1 wherein the compound is an extract of olive oil, cocoa bean, Camellia, licorice, sea whip, aloe vera, chamomile, a derivative thereof or a combination thereof.
8. The method of claim 1 wherein the composition comprises a delivery vehicle.
9. The method of claim 1 wherein the composition further comprises glutaraldehyde.
10. The method of claim 1 wherein the composition further comprises gallic acid scavenger, a lipid lowering medication, an anti-bacterial agent, or an anti-fungal agent.
11. A composition for stabilizing elastin containing connective tissue in a living subject comprising a compound and a delivery vehicle wherein the compound is at an effective concentration and interacts with elastin in the connective tissue in vivo to stabilize the connective tissue and the composition has a pH between about 4 and about 9.
12. The composition of claim 11 wherein the connective tissue is within a blood vessel.
13. The composition of claim 11 wherein the compound is a phenolic compound.
14. The composition of claim 11 wherein the compound has a hydrophobic region and a plurality of functional groups capable of hydrogen bonding.
15. The composition of claim 11 wherein the compound is a flavonoid or a flavonoid derivative, a flavolignan or a flavolignan derivative, a phenolic rhizome or a phenolic rhizome derivative, a flavan-3-ol or a flavan-3-ol derivative, a tannin or a tannin derivative, an ellagic acid or an ellagic acid derivative, a procyanidin or a procyanidin derivative, a combination thereof, or a pharmaceutically acceptable salt thereof.
16. The composition of claim 11 wherein the compound is anthocyanins, quercetin, (+)-catechin, (\u2212)-epicatechin, tannic acid, pentagalloylglucose, nobotanin, epigallocatechin gallate, gallotannins, a combination thereof, or a pharmaceutically acceptable salt thereof.
17. The composition of claim 11 wherein the compound is an extract of olive oil, cocoa bean, Camellia, licorice, sea whip, aloe vera, chamomile, a derivative thereof or a combination thereof.
18. The composition of claim 11 further comprises glutaraldehyde.
19. The composition of claim 11 further comprises a gallic acid scavenger, a lipid lowering medication, an anti-bacterial agent, or an anti-fungal agent.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for:
increasing the water use efficiency of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
increasing the rate of growth or biomass production in a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
enhancing the carbon dioxide (CO2) sensitivity of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, plant part or a plant;
down-regulating or decreasing carbon dioxide (CO2) andor water exchange in a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
decreasing the uptake of carbon dioxide (CO2) of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, plant part or a plant; or
increasing the drought tolerance of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant; or
decreasing the heat resistance or tolerance (e.g., under conditions of drought or increased atmospheric carbon dioxide) of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
comprising:
(a) in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant, increasing the expression andor activity of:
(1) a serine endopeptidase, an apoplastic subtilisin-like serine endopeptidase like protein, an ATSBT5.2-like protein, or a subtilisin-like serine endopeptidase family protein, which is capable of cleaving or cleaves an EPF2 protein (Epidermal patterning factor 2) in a manner such that it facilitates EPF2 binding to an ERECTA receptor,
(2) a serine endopeptidase, an apoplastic subtilisin-like serine endopeptidase like protein, an ATSBT5.2-like protein, or a subtilisin-like serine endopeptidase family protein, gene, cDNA or mRNA (message) encoding a polypeptide with a serine endopeptidase, an apoplastic subtilisin-like serine endopeptidase like protein, an ATSBT5.2-like protein, or a subtilisin-like serine endopeptidase family protein activity, or
(3) a combination of (1) and (2);
(b) the method of (a), wherein the increasing of expression andor activity of the serine endopeptidase, apoplastic subtilisin-like serine endopeptidase like protein, ATSBT5.2-like protein, or subtilisin-like serine endopeptidase family protein, is by:
(1) providing a heterologous serine endopeptidase, apoplastic subtilisin-like serine endopeptidase like protein, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase expressing nucleic acid, wherein optionally the nucleic acid is a gene, cDNA or message, and expressing the gene, cDNA, message andor protein in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
(2) increasing of expression andor activity of a homologous serine endopeptidase-expressing, ATSBT5.2-expressing, subtilisin-like serine endopeptidase family protein-expressing, or endopeptidase-expressing nucleic acid, wherein optionally the nucleic acid is a gene, cDNA or message; or,
(3) a combination of (1) and (2);
thereby:
increasing the water use efficiency of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
increasing the rate of growth or biomass production in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
enhancing the carbon dioxide (CO2) sensitivity of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
down-regulating or decreasing carbon dioxide (CO2) andor water exchange in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
decreasing the uptake of carbon dioxide (CO2) in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
increasing the drought tolerance of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant; or
decreasing the heat resistance or tolerance of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant,
wherein optionally the plant is, or the guard cell, plant cell, plant part or plant organ, is isolated andor derived from: (i) a dicotyledonous or monocotyledonous plant; (ii) wheat, oat, rye, barley, rice, sorghum, maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or, (c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Maniotahot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
2. A method for:
up-regulating or increasing carbon dioxide (CO2) andor water exchange in a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
decreasing the water use efficiency of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
decreasing or desensitizing the carbon dioxide (CO2) sensitivity of a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
upregulating or increasing carbon dioxide (CO2) andor water exchange in a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
increasing the uptake of CO2 in a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant;
decreasing drought tolerance in a guard cell, a root cell, a stomatal lineage stage-specific cell, a plant leaf, a plant organ, a plant part or a plant; or
increasing the heat resistance or tolerance (e.g., under conditions of drought or increased atmospheric carbon dioxide);
comprising:
(a) in a cell of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant, decreasing the expression andor activity of:
(1) a nucleic acid expressing a serine endopeptidase, an apoplastic subtilisin-like serine endopeptidase like protein, an ATSBT5.2-like protein, or a subtilisin-like serine endopeptidase family protein, which is capable of cleaving or cleaves EPF2 protein (Epidermal patterning factor 2) in a manner such that it facilitates EPF2 binding to an ERECTA receptor; or
(2) a serine endopeptidase, an apoplastic subtilisin-like serine endopeptidase like protein, an ATSBT5.2-like protein, an apoplastic subtilisin-like serine endopeptidase or an endopeptidase, or
a subtilisin-like serine endopeptidase family protein, gene, cDNA or mRNA (message) encoding a polypeptide with a serine endopeptidase, an ATSBT5.2-like protein, an apoplastic subtilisin-like serine endopeptidase or an endopeptidase activity;
(b) the method of (a), wherein the decreasing of expression andor activity of the serine endopeptidase, apoplastic subtilisin-like serine endopeptidase-like protein, ATSBT5.2-like protein or endopeptidase, is by:
(1) providing a heterologous antisense, iRNA, miRNA or artificial microRNA (miRNA) inhibitory to: a serine endopeptidase-encoding, an ATSBT5.2-like protein-encoding, an apoplastic subtilisin-like serine endopeptidase-like protein-encoding, subtilisin-like serine endopeptidase family protein-encoding or endopeptidase-encoding nucleic acid, or to decrease or abrogate the expression or activity of a gene, cDNA or mRNA, or any nucleic acid or compound inhibitory to the expression of the serine endopeptidase, ATSBT5.2-like protein, apoplastic subtilisin-like serine endopeptidase-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase; and, expressing the inhibitory nucleic acid or compound, or the heterologous antisense, iRNA, miRNA or artificial microRNA (miRNA), in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
(2) decreasing of expression andor activity of a homologous serine endopeptidase-encoding, ATSBT5.2-like protein-encoding, apoplastic subtilisin-like serine endopeptidase-like protein-encoding, subtilisin-like serine endopeptidase family protein-encoding or endopeptidase-encoding nucleic acid, wherein optionally the nucleic acid is a gene, cDNA or mRNA; or,
(3) a combination of (1) and (2);
thereby:
up-regulating or increasing carbon dioxide (CO2) andor water exchange in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
decreasing the water use efficiency of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
increasing the rate of growth or biomass production in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
decreasing or desensitizing the carbon dioxide (CO2) sensitivity of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
up-regulating or increasing carbon dioxide (CO2) andor water exchange in the guard cell of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
increasing the uptake of CO2 in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
decreasing the drought tolerance of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant; or
increasing the heat resistance or tolerance of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant,
wherein optionally the plant is, or the guard cell, plant cell, plant part or plant organ, is isolated andor derived from: (i) a dicotyledonous or monocotyledonous plant; (ii) wheat, oat, rye, barley, rice, sorghum, maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or, (c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Maniotahot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
3. The method of claim 1, wherein the serine endopeptidase, ATSBT5.2-like protein or subtilisin-like serine endopeptidase family protein, comprises an amino acid sequence having between about 75% to 100% sequence identity, or 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity, with
(a) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72;
(b) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2 or SEQ ID NO:4;
(c) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:17, SEQ ID NO:22, SEQ ID NO:40 or SEQ ID NO:53; or
(d) a processed form or a \u201cmature\u201d form, or a form lacking a signal sequence of a protein of (a), (b) or (c).
4. The method of claim 1, wherein the serine endopeptidase, ATSBT5.2-like protein or subtilisin-like serine endopeptidase family protein, is encoded by a nucleotide sequence comprising or consisting of:
(a) the nucleotide sequences of SEQ ID NO:1; or
(b) any of the nucleotide sequences encoding any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72;
(c) any of the nucleotide sequences encoding an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:17, SEQ ID NO:22, SEQ ID NO:40 or SEQ ID NO:53; or
(d) a processed form or a \u201cmature\u201d form, or a form lacking a signal sequence of a protein of (a), (b) or (c).
5. (canceled)
6. The method of claim 1, wherein the serine endopeptidase-expressing, ATSBT5.2-like protein-expressing, subtilisin-like serine endopeptidase family protein-expressing or endopeptidase-expressing nucleic acid, is operably linked to a plant expressible promoter, an inducible promoter, a constitutive promoter, a root specific promoter, a stomatal lineage stage-specific cell specific promoter, a guard cell specific promoter, a drought-inducible promoter, a stress-inducible promoter or a guard cell active promoter, wherein optionally the nucleic acid is a gene, a cDNA or an mRNA.
7. The method of claim 2, wherein the:
up-regulating or increasing carbon dioxide (CO2) andor water exchange in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
decreasing of the water use efficiency of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant; or
decreasing or desensitizing of the carbon dioxide (CO2) sensitivity of the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant; or
upregulating or increasing of the carbon dioxide (CO2) andor water exchange in the guard cell, root cell, stomatal lineage stage-specific cell, plant leaf, plant organ, plant part or plant;
comprises:
(1) providing:
(i) a nucleic acid inhibitory to the expression of a serine endopeptidase-expressing, ATSBT5.2-like protein-expressing, subtilisin-like serine endopeptidase family protein-expressing or endopeptidase-expressing nucleic acid; andor
(ii) a nucleic acid inhibitory to the expression of a serine endopeptidase, an ATSBT5.2-like protein, a subtilisin-like serine endopeptidase family protein, or an endopeptidase gene, cDNA or mRNA,
wherein optionally the inhibitory nucleic acid is an antisense, an iRNA, an siRNA, a micro RNA or miRNA or an artificial micro RNA;
(2) expressing the nucleic acid inhibitory to the expression of the serine endopeptidase-expressing, the ATSBT5.2-like protein-expressing, the subtilisin-like serine endopeptidase family protein-expressing or the endopeptidase-expressing nucleic acid, gene, cDNA or mRNA in a guard cell; andor, expressing a nucleic acid inhibitory to the expression of the serine endopeptidase-expressing, the ATSBT5.2-like protein-expressing, the subtilisin-like serine endopeptidase family protein-expressing or the endopeptidase-expressing nucleic acid, gene, cDNA or mRNA or transcript,
thereby up-regulating or increasing carbon dioxide (CO2) andor water exchange in a guard cell; decreasing the water use efficiency of a guard cell, a plant, plant leaf, plant organ or plant part; or decreasing (desensitizing) the carbon dioxide (CO2) sensitivity of a plant, plant leaf, plant organ or plant part; or upregulating or increasing carbon dioxide (CO2) andor water exchange in a guard cell of a plant, plant leaf, plant organ or plant part.
8. The method of claim 2, wherein the nucleic acid inhibitory to the expression of a CO2 sensor protein-expressing nucleic acid comprises:
(a) a nucleotide sequence of at least about 11, 12, 13, 14, 15, 16, 17, 18, or 19 or more nucleotides having at least about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% or more sequence identity with a nucleotide sequence encoding a serine endopeptidase, a ATSBT5.2-like polypeptide, a subtilisin-like serine endopeptidase family protein, or an endopeptidase,
and optionally the nucleic acid encodes a polypeptide comprising an amino acid sequence having between about 75% and 100% sequence identity, or 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity, with
(i) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72; or
(ii) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2 or SEQ ID NO:4; or
(iii) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:17, SEQ ID NO:22, SEQ ID NO:40 or SEQ ID NO:53; or
(b) a partial or complete complementary sequence of the nucleotide sequence (a).
9. The method of claim 1, wherein the nucleic acid inhibitory to the expression of a serine endopeptidase-expressing, a ATSBT5.2-like polypeptide-expressing, a subtilisin-like serine endopeptidase family protein-expressing, or an endopeptidase-expressing nucleic acid comprises:
(a) a nucleotide sequence of at least about 11, 12, 13, 14, 15, 16, 17, 18, or 19 or more nucleotides having at least about 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% or more sequence identity with a nucleotide sequence of SEQ ID NO:1, or a nucleic acid encoding an amino acid sequence of any one of SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, SEQ ID NO:15, SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, SEQ ID NO:25, SEQ ID NO:27, SEQ ID NO:29, SEQ ID NO:31, SEQ ID NO:33, SEQ ID NO:35, SEQ ID NO:37, SEQ ID NO:39, SEQ ID NO:41, SEQ ID NO:43 or SEQ ID NO:45; or
(b) a partial or complete complementary sequence of the nucleotide sequence (a).
10-21. (canceled)
22. A method for increasing the number of stomatal pores compared to the total number of cells, or increasing the stomatal density, stomatal index andor stomatal size, in a plant, plant part, a plant organ, a plant leaf, comprising:
decreasing the expression of a serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein-encoding gene, cDNA or mRNA or transcript in the plant, guard cell, plant cell, plant leaf, plant organ or plant part, by expressing a nucleic acid inhibitory to the expression of the serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein-expressing nucleic acid, gene, cDNA or mRNA or transcript, as described in claim 2, in the plant, guard cell, plant cell, plant leaf, plant organ, or plant part,
thereby increasing the stomatal density, stomatal index andor stomatal size in the epidermis of the plant, plant part, plant organ or plant leaf,
and optionally the decreasing of the expression occurs in the plant guard cell or in a precursor cell thereof.
23. A method for decreasing the number of stomatal pores compared to the total number of cells, or decreasing the stomatal density, stomatal index andor stomatal size, in a plant, plant part, a plant organ, a plant leaf, comprising:
expressing or increasing the expression of a serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein-encoding gene, cDNA or mRNA or transcript, as described in claim 1 in the plant, guard cell, plant cell, plant leaf, plant organ or plant part
thereby decreasing the stomatal density, stomatal index andor stomatal size in the epidermis of the plant, plant part, plant leaf, plant organ,
and optionally the expression or increase in expression occurs in the plant guard cell.
24-28. (canceled)
29. A transgenic guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ, comprising:
(a) an heterologous serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein-expressing nucleic acid; or
wherein optionally the nucleic acid, gene or transcript is operably linked to a plant expressible promoter, an inducible promoter, a constitutive promoter, a root specific promoter, a stomatal lineage stage-specific cell specific promoter, a guard cell specific promoter, a drought-inducible promoter, a stress-inducible promoter or a guard cell active promoter;
and optionally the nucleic acid, gene or transcript is stably integrated into the genome of the guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ, or is contained in an episomal vector in the guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ.
30. A transgenic guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ, comprising:
(a) (1) a heterologous nucleic acid that is inhibitory to an serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein-expressing nucleic acid;
wherein optionally the inhibitory nucleic acid is operably linked to a plant expressible promoter, an inducible promoter, a constitutive promoter, a root specific promoter, a stomatal lineage stage-specific cell specific promoter, a guard cell specific promoter, a drought-inducible promoter, a stress-inducible promoter or a guard cell active promoter;
and optionally the inhibitory nucleic acid is stably integrated into the genome of the guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ, or is contained in an episomal vector in the guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ,
and optionally the inhibitory nucleic acid comprises an antisense RNA, siRNA, miRNA or an iRNA or an artificial micro RNA.
31. (canceled)
32. The guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ of claim 29, wherein the nucleic acid encoding a ATSBT5.2-like protein encodes a serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein comprising an amino acid sequence having between 75% and 100% sequence identity, or 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity, with
(a) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72;
(b) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2 or SEQ ID NO:4;
(c) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:17, SEQ ID NO:22, SEQ ID NO:40 or SEQ ID NO:53; or
(d) a processed form or a \u201cmature\u201d form, or a form lacking a signal sequence of a protein of (a), (b) or (c).
33. The guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ of claim 29, wherein the serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein is encoded by a nucleotide sequence of SEQ ID NO:1.
34. The guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ of claim 30, wherein the nucleic acid, which when transcribed yields an inhibitory nucleic acid to the expression of a ATSBT5.2-like protein-expressing nucleic acid comprises a nucleotide sequence of at least 11, 12, 13, 14, 15, 16, 17, 18, or 19 or more nucleotides having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% or more sequence identity with a nucleotide sequence encoding serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein comprising an amino acid sequence having between 75% and 100% sequence identity, or 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity, with
(a) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72,
wherein the inhibitory nucleic acid comprises an inhibitory ribonucleic acid, an siRNA, an miRNA or an artificial micro RNA; or
(b) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2 or SEQ ID NO:4; or
(c) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:17, SEQ ID NO:22, SEQ ID NO:40 or SEQ ID NO:53; or
(d) a complete or partial complement thereof.
35-37. (canceled)
38. The guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ of claim 29, wherein the plant is or the guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ is isolated andor derived from: (i) a dicotyledonous or monocotyledonous plant; (ii) wheat, oat, rye, barley, rice, sorghum, maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), a cruciferous plant, a cauliflower, rape (or rapa or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or, (c) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Maniotahot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
39. A method for increasing or decreasing the stomatal cell density in a plant, plant part or plant organ, comprising providing cells of a guard cell, plant, plant cell, plant tissue, plant seed or fruit, plant part or plant organ with a recombinant gene, wherein the recombinant gene is selected from an expression increasing recombinant gene or an expression inhibiting recombinant gene as described in claim 29 for
a. regulating carbon dioxide and water exchange in a plant;
b. regulating water uptake or water loss in a plant;
c. regulating water use efficiency or drought tolerance in a plant;
d. regulating biomass accumulation in a plant; or
e. regulating leaf temperature and transpiration in a plant.
40-42. (canceled)
43. A chimeric DNA comprising the following operably linked fragments:
(a) a plant-expressible promoter;
(b) a DNA region heterologous to said plant-expressible promoter which when transcribed yields an RNA, said RNA either:
i. encoding an amino acid sequence having between 75% and 100% sequence identity, or 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity, with an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72; or
ii. comprising at least 19 consecutive nucleotides having at least 94% sequence identity with a nucleotide sequence encoding a polypeptide having between 75% and 100% sequence identity, or 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete (100%) sequence identity, with an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72; and optionally also comprising at least the complement of said 19 consecutive nucleotides; and
(c) optionally a transcription termination and polyadenylation signal functional in plant cells.
44. A method for making a plant cell with altered stomatal density, stomatal index andor stomatal size, said method comprising providing a cell of a plant as set forth in claim 29.
45. (canceled)
46. A method for altering the stomatal cell density, comprising selecting a plant comprising a substitution, deletion or insertion of one or more nucleotides in an endogenous gene encoding an ATSBT5.2-like protein.
47-50. (canceled)
51. A plant comprising a modified endogenous gene encoding a serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein comprising an amino acid sequence having between about 75% and 100% sequence identity with
(a) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, SEQ ID NO:30, SEQ ID NO:31, SEQ ID NO:32, SEQ ID NO:33, SEQ ID NO:34, SEQ ID NO:35, SEQ ID NO:36, SEQ ID NO:37, SEQ ID NO:38, SEQ ID NO:39, SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, SEQ ID NO:45, SEQ ID NO:46, SEQ ID NO:47, SEQ ID NO:48, SEQ ID NO:49, SEQ ID NO:50, SEQ ID NO:51, SEQ ID NO:52, SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55, SEQ ID NO:56, SEQ ID NO:57, SEQ ID NO:58, SEQ ID NO:59, SEQ ID NO:60, SEQ ID NO:61, SEQ ID NO:62, SEQ ID NO:63, SEQ ID NO:64, SEQ ID NO:65, SEQ ID NO:66, SEQ ID NO:67, SEQ ID NO:68, SEQ ID NO:69, SEQ ID NO:70, SEQ ID NO:71 or SEQ ID. No.72; or
(b) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:2 or SEQ ID NO:4; or
(c) an amino acid sequence comprising or consisting any one of the amino acid sequences of SEQ ID NO:5, SEQ ID NO:8, SEQ ID NO:17, SEQ ID NO:22, SEQ ID NO:40 or SEQ ID NO:53;
wherein said endogenous gene comprises a substitution, deletion or insertion of one or more nucleotides in an endogenous gene encoding a serine endopeptidase, ATSBT5.2-like protein, subtilisin-like serine endopeptidase family protein or endopeptidase protein and wherein said plant and wherein said substitution, deletion or insertion results in the translation of a non-functional protein or a truncated protein or no protein at all from said endogenous gene,
and optionally the plant is selected from
(a) wheat, oat, rye, barley, rice, sorghum, maize (corn), tobacco, a legume, a lupins, potato, sugar beet, pea, bean, soybean (soy), oilseed rape, a cauliflower, rape (or raga or canola), cane (sugarcane), flax, cotton, palm, sugar beet, peanut, a tree, a poplar, a lupin, a silk cotton tree, desert willow, creosote bush, winterfat, balsa, ramie, kenaf, hemp, roselle, jute, or sisal abaca; or,
(b) a species from the genera Anacardium, Arachis, Asparagus, Atropa, Avena, Brassica, Citrus, Citrullus, Capsicum, Carthamus, Cocos, Coffea, Cucumis, Cucurbita, Daucus, Elaeis, Fragaria, Glycine, Gossypium, Helianthus, Heterocallis, Hordeum, Hyoscyamus, Lactuca, Linum, Lolium, Lupinus, Lycopersicon, Malus, Maniotahot, Majorana, Medicago, Nicotiana, Olea, Oryza, Panieum, Pannisetum, Persea, Phaseolus, Pistachia, Pisum, Pyrus, Prunus, Raphanus, Ricinus, Secale, Senecio, Sinapis, Solanum, Sorghum, Theobromus, Trigonella, Triticum, Vicia, Vitis, Vigna or Zea.
52-80. (canceled)