What is claimed is:
1. An isolated nucleic acid comprising a sequence as set forth in SEQ ID NO:1 and variants thereof having at least 70% identity to SEQ ID NO:1 and encoding a polypeptide having polymerase activity at a temperature in a range from about 90 C. to 113 C.
2. The isolated nucleic acid of claim 1, wherein the polymerase activity is retained at the temperature for four or more hours.
3. The isolated nucleic acid of claim 1, comprising a sequence as set forth in SEQ ID NO: 1, sequences substantially identical thereto, and sequences complementary thereto.
4. An isolated nucleic acid that hybridizes to a nucleic acid of claim 1 under conditions of high stringency.
5. An isolated nucleic acid that hybridizes to a nucleic acid of claim 1 under conditions of moderate stringency.
6. An isolated nucleic acid that hybridizes to a nucleic acid of claim 1 under conditions of low stringency.
7. An isolated nucleic acid having at least 70% homology to the nucleic acid of claim 1 as determined by analysis with a sequence comparison algorithm.
8. An isolated nucleic acid having at least 80% homology to the nucleic acid of claim 1 as determined by analysis with a sequence comparison algorithm.
9. An isolated nucleic acid having at least 90% homology to the nucleic acid of claim 1 as determined by analysis with a sequence comparison algorithm.
10. An isolated nucleic acid having at least 95% homology to the nucleic acid of claim 1 as determined by analysis with a sequence comparison algorithm.
11. The isolated nucleic acid of claim 7, 8, 9, or 10, wherein the sequence comparison algorithm is FASTA version 3.0t78 with the default parameters.
12. An isolated nucleic acid comprising at least 10 consecutive bases of a sequence as set forth in SEQ ID NOs: 1, sequences substantially identical thereto, and sequences complementary thereto.
13. An isolated nucleic acid having at least 70% homology to the nucleic acid of claim 11 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t78 with the default parameters.
14. An isolated nucleic acid having at least 80% homology to the nucleic acid of claim 11 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
15. An isolated nucleic acid having at least 90% homology to the nucleic acid of claim 11 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
16. An isolated nucleic acid encoding a polypeptide having a sequence as set forth in SEQ ID NO:2, and sequences substantially identical thereto.
17. An isolated nucleic acid encoding a polypeptide comprising at least 10 consecutive amino acids of a polypeptide having a sequence selected from the group consisting of SEQ ID NO: 2, and sequences substantially identical thereto.
18. A purified polypeptide having at least 70% homology to the polypeptide of claim 16 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
19. A purified polypeptide having at least 80% homology to the polypeptide of claim 16 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
20. A purified polypeptide having at least 90% homology to the polypeptide of claim 16 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
21. A purified polypeptide having at least 95% homology to the polypeptide of claim 16 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
22. A purified polypeptide having at least 70% homology to the polypeptide of claim 17 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
23. A purified polypeptide having at least 80% homology to the polypeptide of claim 17 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t78 with the default parameters.
24. A purified polypeptide having at least 90% homology to the polypeptide of claim 17 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
25. A purified polypeptide having at least 95% homology to the polypeptide of claim 17 as determined by analysis with a sequence comparison algorithm or FASTA version 3.0t 78 with the default parameters.
26. A purified polypeptide having a sequence as set forth in SEQ ID NO: 2, and sequences substantially identical thereto, wherein the polypeptide has polymerase activity at temperatures in the range from about 70 C. up to about 113 C.
27. An assay for identifying functional polypeptide fragments or variants encoded by fragments of SEQ ID NO: 1, and sequences substantially identical thereto, that retain the polymerase function of the polypeptide of SEQ ID NO: 2, and sequences substantially identical thereto, said assay comprising:
utilizing a polypeptide encoded by a nucleic acid having at least 70% homology to SEQ ID NO: 1, and sequences substantially identical thereto, or polypeptide fragment or variant encoded by SEQ ID NO: 1, to effect DNA polymerase activity in a PCR amplification at extreme high temperature for four or more hours and under conditions that allow said polypeptide or fragment or variant to function, and
detecting formation of an amplification product, wherein formation of the amplification product is indicative of a functional DNA polymerase polypeptide or fragment or variant.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An information processing system using biometrics authentication for individual authentication and accessing of information relating to the individual of said individual authentication is permitted, comprising:
a detection device, which detects the relevant portion of said biometrics characteristics;
an input unit, which inputs a group number of said user;
a biometrics database file, which stores said group number, a plurality of individual identification numbers belonging to the group of the group number, and biometrics characteristic data linked to said individual identification numbers;
an individual database file, which stores individual information for said user linked to said individual identification numbers; and
a control unit which reads the corresponding individual identification numbers and said biometrics characteristic data from said biometrics database file according to input said group number, and performs processing to verify said biometrics characteristic data obtained from said biometrics characteristic portion detected by said detection device against said read-out biometrics characteristic data;
wherein said control unit executes access of said individual database file using said individual identification number linked to said biometrics characteristic data for which the result of said verification is satisfactory.
2. The information processing system using biometrics authentication according to claim 1, wherein said control unit comprises:
a terminal device connected to said detection device and to said input unit; and
server connected to said biometrics database file and to said individual database file,
and wherein said terminal device and said server are connected.
3. The information processing system using biometrics authentication according to claim 2,
wherein said terminal device transmits said input group number and biometrics characteristic data detected by said detection device to said server, and
wherein said server reads from said biometrics database file the corresponding individual identification numbers and said biometrics characteristic data using said input group number, performs verification processing against said biometrics characteristic data detected by said detection device, uses said individual identification number linked to said biometrics characteristic data for which said verification result is satisfactory to execute access of said individual database file, and transmits said accessed individual information to said terminal device.
4. The information processing system using biometrics authentication according to claim 2,
wherein said terminal device transmits said input group number to said server, and
wherein said server uses said input group number to read and transmit to said terminal device the corresponding individual identification numbers and said biometrics characteristic data from said biometrics database file, and
said terminal device performs verification processing against said biometrics characteristic data detected by said detection device and notifies said server of said verification result, and
said server uses said individual identification number linked to said biometrics characteristic data for which said verification result is satisfactory to execute access of said individual database file and transmit said accessed individual information to said terminal device.
5. The information processing system using biometrics authentication according to claim 1, wherein said control unit receives the group number input from said input unit, the individual identification number and the biometrics characteristic data detected by said detection device, and registers said group number, said individual identification number, and said biometrics characteristic data in said biometrics database file.
6. The information processing system using biometrics authentication according to claim 1, wherein said control unit uses said input group number to perform processing to verify a plurality of sets of said biometrics characteristic data which are read from said biometrics database file, against said biometrics characteristic data obtained from said biometrics characteristic portion detected by said detection device, and identifies the individual identification number belonging to said group.
7. The information processing system using biometrics authentication according to claim 1, wherein said control unit extracts said biometrics characteristic data from said biometrics characteristic portion detected by said detection device.
8. The information processing system using biometrics authentication according to claim 1, wherein said detection device comprises a device which detects blood vessel images in the body.
9. The information processing system using biometrics authentication according to claim 8, wherein said detection device comprises an image capture unit which captures images of blood vessels in a hand of said body.
10. The information processing system using biometrics authentication according to claim 1, wherein said control unit uses said accessed individual information to execute processing of individual information.