1. A protective cover, comprising a cover base and a protective lid, said cover base and said protective lid forming a housing space, said cover base holding and fastening a portable electronic device inside said housing space, said cover base and said protective lid being engaged by at least a buckle element, said buckle element comprising a plug connected to an end of a soft element from a side of said protective lid and a socket formed at location corresponding to said plug on a side of said cover base, said plug of said buckle element able to repeatedly plug into and unplug from said socket so that said protective lid and cover base able to engaged or disengaged for different use purposes.
2. The protective cover as claimed in claim 1, wherein said plug has a groove close to both sides to form a resilient protruding block on both sides, inner shape of said socket matches said plug so that said plug and repeatedly plug into and unplug from said socket.
3. The protective cover as claimed in claim 1, wherein said cover base comprises at least two clap elements, said clap elements are bended and distributed surrounding circumference of said cover base.
4. The protective cover as claimed in claim 3, wherein said cover base comprises at least a stop wall, located at least one place of circumference of said cover base, said stop wall and said clap elements are preferably not located on the same side of said cover base.
5. The protective cover as claimed in claim 1, wherein said protective lid comprises a hold element on a side, located opposite to said buckle element of said protective lid.
6. The protective cover as claimed in claim 1, wherein back surface of said cover base is coupled to a support plate, when flipped, said support plate forms an angle with said back of said cover base so that said cover base can stand in either horizontal mode or horizontal mode.
7. A protective cover, comprising a cover base and a protective lid, said cover base and said protective lid forming a housing space, said cover base holding and fastening a portable electronic device inside said housing space, said cover base and said protective lid being engaged by at least a buckle element, said buckle element able to repeatedly plug and unplug so that said protective lid and cover base able to engaged or disengaged for different use purposes, back surface of said cover base being coupled to a support plate, said support plate forming an angle with said back surface of said cover base when flipped so that said cover base able to stand in either horizontal or vertical modes.
8. The protective cover as claimed in claim 7, wherein said support plate is coupled to a fix element, said fix element comprises a connection groove, shaped to match a connection head of an apparatus support device used in vehicle.
9. The protective cover as claimed in claim 7, wherein said buckle element comprises a plug connected to an end of a soft element from a side of said protective lid and a socket formed at location corresponding to said plug on a side of said cover base, said plug can repeatedly plug into or unplug from said socket.
10. The protective cover as claimed in claim 7, wherein said cover base comprises at least two clap elements, said clap elements are bended and distributed surrounding circumference of said cover base.
11. The protective cover as claimed in claim 10, wherein said cover base comprises at least a stop wall, located at least one place of circumference of said cover base, said stop wall and said clap elements are preferably not located on the same side of said cover base.
12. The protective cover as claimed in claim 7, wherein said protective lid comprises a hold element on a side, located opposite to said buckle element of said protective lid.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for inducing increasing and sustained levels of in vivo gene expression of an exogenous nucleic acid in a mammal over a prolonged period of time, comprising contacting a non-muscular tissue of said mammal with a composition comprising said exogenous nucleic acid, a histone, and an amphipathic compound, wherein increasing and sustained levels of gene expression of said exogenous nucleic acid is detected in said mammal for at least one week after contacting said tissue with said composition.
2. The method of claim 1, wherein said tissue is neuronal tissue.
3. The method of claim 1, wherein said tissue is a central nervous system (CNS) tissue.
4. The method of claim 1, wherein said tissue comprises a post-mitotic neuronal cell.
5. The method of claim 1, wherein said tissue comprises a cortical neuronal cell.
6. The method of claim 1, wherein said tissue comprises a hippocampal neuronal cell, a glial cell, or a vascular endothelial cell.
7. The method of claim 1, wherein increasing and sustained levels of gene expression in said tissue is detected in vivo for at least two weeks after contacting said tissue with said composition.
8. The method of claim 1, wherein increasing and sustained levels of gene expression in said tissue is detected in vivo for at least four weeks after contacting said tissue with said composition.
9. The method of claim 1, wherein increasing and sustained levels of gene expression in said tissue is detected in vivo for at least eight weeks after contacting said tissue with said composition.
10. The method of claim 1, wherein said composition is in the form of a liposome.
11. The method of claim 10, wherein said liposome is neutral or cationic.
12. The method of claim 10, wherein said lipsome is anionic.
13. The method of claim 1, wherein said histone is selected from the group consisting of H1, H2A, H2B, H3, and H4.
14. The method of claim 1, wherein said composition further comprises a nuclear localizing signal.
15. The method of claim 1 wherein the amphipathic compound is a non-natural polyamine having a hydrophobic moiety, wherein said hydrophobic moiety is selected from the group consisting of a C6-C24 alkane, C6-C24 alkene, sterol, steroid, lipid, fatty acid, and hydrophobic hormone.
16. The method of claim 1, wherein said nucleic acid is an AD7c-NTP antisense molecule or a nitric oxide synthase III antisense molecule.
17. The method of claim 16, wherein said nucleic acid comprises a sequence complementary to the nucleotide sequence of SEQ ID NO:1.