1. An isolated polynucleotide encoding a protein selected from the group consisting of:
(A) a protein comprising the amino acid sequence of SEQ ID NO:5, and
(B) a protein comprising the amino acid sequence of SEQ ID NO:5, but which includes substitution, deletion, insertion andor addition of one to 20 amino acids, and wherein said protein is able to catalyze a reaction in which an \u03b1-amino acid of formula (1):
is reacted with an aldehyde of formula (II):
to form a serine derivative of formula (III):
wherein R1 is selected from the group consisting of an alkyl group having 1 to 7 carbon atoms, an aryl group having 6 to 14 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, an aralkyl group having 7 to 19 carbon atoms, an alkoxyalkyl group having 2 to 11 carbon atoms, a group containing a hetero atom in the carbon skeleton thereof, and a group containing a carbon-carbon unsaturated bond in the carbon skeleton thereof, wherein these groups may be straight or branched, may have alicyclic hydrocarbon structure and may further have a substituent,
wherein R2 is selected from the group consisting of hydrogen, an alkyl group having 1 to 7 carbon atoms, an aryl group having 6 to 14 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, an aralkyl group having 7 to 19 carbon atoms, an alkoxyalkyl group having 2 to 11 carbon atoms, a group containing a hetero atom in the carbon skeleton thereof, and a group containing a carbon-carbon unsaturated bond in the carbon skeleton thereof, wherein these groups may be straight or branched, may have an alicyclic hydrocarbon structure and may further have a substituent.
2. An isolated polynucleotide selected from the group consisting of:
(a) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:4, and
(b) a polynucleotide that hybridizes with a polynucleotide having a nucleotide sequence complementary to the nucleotide sequence of SEQ ID NO:4 under stringent conditions and encodes a protein which is able to catalyze a reaction in which an \u03b1-amino acid of formula (I):
is reacted with an aldehyde of formula (II):
to form a serine derivative of formula (III):
wherein R1 is selected from the group consisting of an alkyl group having 1 to 7 carbon atoms, an aryl group having 6 to 14 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, an aralkyl group having 7 to 19 carbon atoms, an alkoxyalkyl group having 2 to 11 carbon atoms, a group containing a hetero atom in the carbon skeleton thereof, and a group containing a carbon-carbon unsaturated bond in the carbon skeleton thereof, wherein these groups may be straight or branched, may have an alicyclic hydrocarbon structure and may further have a substituent,
wherein R2 is selected from the group consisting of hydrogen, an alkyl group having 1 to 7 carbon atoms, an aryl group having 6 to 14 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, an aralkyl group having 7 to 19 carbon atoms, an alkoxyalkyl group having 2 to 11 carbon atoms, a group containing a hetero atom in the carbon skeleton thereof, and a group containing a carbon-carbon unsaturated bond in the carbon skeleton thereof, wherein these groups may be straight or branched, may have an alicyclic hydrocarbon structure and may further have a substituent,
wherein said stringent conditions comprise washing at a salt concentration corresponding to 1\xd7SSC and 0.1% SDS at 60\xb0 C.
3. A cell transformed with the polynucleotide according to claim 1.
4. The cell according to claim 3, wherein the cell is Escherichia coli.
5. The polynucleotide according to claim 1, wherein the protein (B) comprises a substitution of the tyrosine located at position 339 with an amino acid selected from the group consisting of serine, histidine, and asaparagine.
6. The polynucleotide according to claim 1, wherein the protein (B) comprises substitution of the asparagine located at position 19 with a serine.
7. A method for producing a serine derivative comprising reacting an \u03b1-amino acid of formula (I):
with an aldehyde of formula (II):
in a culture comprising the cell according to claim 3 to form the serine derivative of formula (III):
wherein R1 is selected from the group consisting of an alkyl group having 1 to 7 carbon atoms, an aryl group having 6 to 14 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, aralkyl group having 7 to 19 carbon atoms, alkoxyalkyl group having 2 to 11 carbon atoms, a group containing a hetero atom in the carbon skeleton thereof, and a group containing a carbon-carbon unsaturated bond in the carbon skeleton thereof, wherein these groups may be straight or branched, may have an alicyclic hydrocarbon structure and may further have a substituent,
wherein R2 is selected from the group consisting of hydrogen, an alkyl group having 1 to 7 carbon atoms, an aryl group having 6 to 14 carbon atoms, a cycloalkyl group having 3 to 10 carbon atoms, an aralkyl group having 7 to 19 carbon atoms, an alkoxyalkyl group having 2 to 11 carbon atoms, a group containing a hetero atom in the carbon skeleton thereof, and a group containing a carbon-carbon unsaturated bond in the carbon skeleton thereof, wherein these groups may be straight or branched, may have an alicyclic hydrocarbon structure and may further have a substituent.
8. The method for producing the serine derivative according to claim 7, wherein the culture further comprises the \u03b1-amino acid of formula (I) and the aldehyde of formula (II), and the culture is mixed to form the serine derivative of formula (III).
9. The method for producing the serine derivative according to claim 7, wherein the amino acid of formula (I) is an amino acid selected from the group consisting of phenylalanine, leucine, methionine, alanine, cysteine, tryptophan, isoleucine, cyclohexylalanine, 2-amino-n-butyric acid, 2-aminovaleric acid, 2-aminohexanoic acid, and combinations thereof.
10. The method for producing the serine derivative according to claim 7, wherein the amino acid of formula (I) is \u03b1-phenylalanine, and the serine derivative of formula (III) is \u03b1-benzylserine.
11. The method for producing the serine derivative according to claim 7, wherein the amino acid of formula (I) is \u03b1-leucine, and the serine derivative of formula (III) is \u03b1-isobutylserine.
12. The method for producing the serine derivative according to claim 7, wherein the amino acid of formula (I) is \u03b1-methionine, and the serine derivative of formula (III) is \u03b1-methylthioethylserine.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A radiant heating assembly, which comprises a radiant heating panel and tubing, wherein the radiant heating panel is made up of (a) a radiant heat transfer plate having a length and a center line that extends along the length, (b) mounting holes provided in the plate, and (c) an elongated C-shaped receptacle for receiving the tubing, wherein the C-shaped receptacle extends along the center line of the radiant heat transfer plate and is formed from upper and lower arcuate or angled sections, with at least the upper section terminating in an edge, wherein a plurality of depressions extend along an inner surface of the C-shaped receptacle that interfaces with the plate, the lower section of the C-shaped receptacle fixably attached to the plate along the center line of the plate, wherein the radiant heat transfer plate is completely planar except for areas containing mounting holes and the C-shaped receptacle, wherein the tubing is contained within the C-shaped receptacle and deforms to be in contact with inner surfaces of the plurality of depressions.
2. The radiant heating assembly of claim 1, wherein the radiant heating panel is constructed from a thermally conductive material.
3. The radiant heating assembly of claim 2, wherein the radiant heating panel is constructed from aluminum.
4. The radiant heating assembly of claim 3, wherein the radiant heating panel is an extruded, one-piece aluminum panel.
5. The radiant heating assembly of claim 1, wherein the plurality of depressions comprise two or more discontinuous depressions.
6. The radiant heating assembly of claim 1, wherein the plurality of depressions comprise two or more continuous depressions that each extend along the entire length of the inner surface of the lower section of the C-shaped receptacle, wherein each continuous depression is contiguous to at least one other continuous depression.
7. The radiant heating assembly of claim 6, wherein each depression is a U-shaped depression.
8. The radiant heating assembly of claim 7, wherein each U-shaped depression has a width ranging from about 0.13 to about 0.38 millimeters, and a depth ranging from about 0.25 to about 0.76 millimeters.
9. The radiant heating assembly of claim 1, wherein the plurality of depressions comprise two or more adjacent or contiguous depressions that extend across the entire width and along a length of the inner surface of the C-shaped receptacle that interfaces with the plate.
10. The radiant heating assembly of claim 1, wherein both the upper and lower sections of the elongated, C-shaped receptacle terminate in an outwardly flaring edge.
11. The radiant heating assembly of claim 1, wherein the C-shaped receptacle has a thickness of less than about 1.27 millimeters.
12. The radiant heating assembly of claim 11, wherein the thickness of the C-shaped receptacle is about 1.02 millimeters.
13. The radiant heating assembly of claim 1, wherein the tubing is prepared from raised temperature resistant polyethylene.
14. A radiant heating assembly, which comprises a radiant heating panel and tubing, wherein the radiant heating panel is made up of (a) a radiant heat transfer plate having a length and a center line that extends along the length, and (b) an elongated C-shaped receptacle for receiving the tubing, wherein the C-shaped receptacle extends along the center line of the radiant heat transfer plate and is formed from upper and lower arcuate or angled sections, with at least the upper section terminating in an edge, wherein a plurality of depressions extend along an inner surface of the C-shaped receptacle that interfaces with the plate, the lower section of the C-shaped receptacle fixably attached to the plate along the center line of the plate, wherein the tubing is contained within the C-shaped receptacle and deforms to be in contact with inner surfaces of the plurality of depressions.
15. The radiant heating assembly of claim 14, wherein the plurality of depressions comprise two or more adjacent or contiguous depressions that extend across the entire width and along a length of the inner surface of the C-shaped receptacle that interfaces with the plate.
16. The radiant heating assembly of claim 14, wherein the C-shaped receptacle has a thickness of less than about 1.27 millimeters.
17. The radiant heating assembly of claim 16, wherein the thickness of the C-shaped receptacle is about 1.02 millimeters.
18. The radiant heating assembly of claim 14, wherein the tubing is prepared from raised temperature resistant polyethylene.