1. Method for detecting andor quantifying a target nucleotide sequence (2) present in a biological sample, characterized in that it comprises a bringing into contact of the sandwich type of the target nucleotide sequence (2) with a single-stranded trapping nucleotide sequence (5) attached to an insoluble solid support (3), and complementary to a portion (7) of the said target nucleotide sequence (2), and with one or more nucleotide sequences (6, 11) of which at least one is labeled (6), said nucleotide sequence(s) (6, 11) being complementary to another portion (8) of the target nucleotide sequence (2); in that the trapping nucleotide sequence (5) is covalently attached by one of its ends to the solid support (3); comprising a length of between 50 and 300 bases; and in that a portion (10) of the trapping nucleotide sequence (5) which does not hybridize with the portion (7) of the target nucleotide sequence (2) is less than 60 bases, preferably less than 40 bases, more particular less than 20 bases.
2. Method according to claim 1, characterized in that the length of the trapping nucleotide sequence (5) is between 120 and 250 bases.
3. Method according to claim 1 or 2, characterized in that the yield of hybridization of the target nucleotide sequence (2) to the trapping nucleotide sequence (5) is greater than 40%.
4. Method according to any one of the preceding claims, characterized in that a portion (13) of the target nucleotide sequence which does not hybridize with the trapping nucleotide sequence (5) and with the nucleotide sequence(s) labeled (6) or otherwise (11) is less than 60 bases, preferably less than 40 bases.
5. Method according to any one of the preceding claims, characterized in that the solid support (3) is a support chosen from the group consisting of tubes, filters, beads, which may be magnetic, multiwell plates or a mixture thereof.
6. Method according to any one of the preceding claims, characterized in that the target nucleotide sequence (2) is an amplicon resulting from a preliminary amplification by a gene amplification method, preferably chosen from the group consisting of PCR, LCR, CPR, NASBA or ICR.
7. Method according to claim 6, characterized in that the 5 terminal portion (9) of the target sequence (2) not overlapping with the labeled nucleotide sequences (6) overlaps with a primer sequence (12) used for its amplification.
8. Method according to any one of the preceding claims, characterized in that it also comprises a bringing into contact of the sandwich type of a standard nucleotide sequence (1) under the same conditions as the target nucleotide sequences (2) to be detected andor quantified and comprises an additional step of quantification of the ratio between the specific labeling of the target nucleotide sequence (2) and the specific labeling of the standard nucleotide sequence (1).
9. Method according to claim 8, characterized in that it also comprises a step of a preparation of a known quantity of a standard nucleotide sequence (1) possessing at least a portion (A) common to the target nucleotide sequence (2) and a specific portion (B) whose sequence is different and possesses a content of GCAT bases similar, preferably identical, to the sequence of a specific portion (B) of the target nucleotide sequence (2), an optional extraction of the target nucleotide sequence (2) to be quantified from the biological sample and a bringing into contact of the sandwich type of the target (2) and standard (1) nucleotide sequences with trapping nucleotide sequences (5) complementary to the common portion (A) of these two sequences (1, 2) and with labeled nucleotide sequences (6) complementary either to the specific portion (B) of the target nucleotide sequence (2), or to the specific portion (B) of the standard nucleotide sequence (1), and a quantification between the specific labeling of the target nucleotide sequence (2) and the specific labeling of the standard nucleotide sequence (1).
10. Method according to any one of the preceding claims, characterized in that the sandwich hybridization is carried out in two steps.
11. Method according to any one of the preceding claims, characterized in that the attachment of the trapping nucleotide sequence (5) to the solid support (3) is carried out via a 5 terminal phosphate onto an amine functional group of the solid support (3) by reaction with carbodiimide.
12. Method according to any one of the preceding claims, characterized in that the target nucleotide sequence (2) is a DNA.
13. Method according to any one of claims 1 to 11, characterized in that the target nucleotide sequence is an RNA.
14. Kit for detecting andor quantifying, by a sandwich-type hybridization, a target nucleotide sequence (2) comprising a trapping nucleotide sequence (5) attached to an insoluble solid support (3) complementary to a portion (7) of the said target nucleotide sequence (2) and at least one labeled nucleotide sequence (6) complementary to another portion (8) of the target nucleotide sequence (2), characterized in that the said trapping nucleotide sequence (5) is covalently attached by one of its ends to the solid support (3) and has a length of between 50 and 300 bases, and in that the portion (10) of the trapping nucleotide sequence (5) which does not hybridize with a portion (7) complementary to the target nucleotide sequence (2) is less than 60 bases, preferably less than 40 bases, more particularly less than 20 bases.
15. Kit according to claim 14, characterized in that the length of the trapping nucleotide sequence (5) is between 120 and 250 bases.
16. Kit according to claim 14 or 15, characterized in that the solid support (3) is an insoluble solid support chosen from the group consisting of tubes, filters, beads, which may be magnetic, multiwell plates or a mixture thereof.
17. Kit according to any one of claims 14 to 16, characterized in that it comprises a standard nucleotide sequence (1).
18. Kit according to any one of claims 14 to 17, characterized in that the standard nucleotide sequence (1) possesses at least a portion (A) common to the target nucleotide sequence (2) and a specific portion (B) whose sequence is different and possesses a content of GCAT bases similar, preferably identical, to the specific portion (B) of the target nucleotide sequence (2) to be quantified.
19. Kit according to any one of claims 14 to 18, characterized in that the trapping nucleotide sequence (5) is attached by a 5 terminal phosphate onto an amine functional group of the solid support (3) by reaction with carbodiimide.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A computerized method for authenticating an electronic transaction between a user and a computer, the computer being configured to conduct electronic transactions, the method comprising:
receiving a computer-generated transaction identifier from the computer via an electronic data link;
receiving a user-spoken transaction identifier and a user-spoken verification identifier transmitted by the user via a voice connection;
comparing the user-spoken transaction identifier with the computer transaction identifier;
comparing the user-spoken verification identifier with a voice print of the user; and
transmitting an authentication message to the computer if the user-spoken transaction identifier matches the computer-generated transaction identifier and if the user-spoken verification identifier matches the voice print.
2. The method of claim 1, wherein the computer transaction identifier is generated by the computer in response to the electric transaction conducted between the user and the computer.
3. The method of claim 1, further comprising the step of providing the user voice print and user payment information prior to the electronic transaction.
4. The method of claim 3, wherein the user voice print is provided by the user by providing a spoken telephone number to a voice recognition unit.
5. The method of claim 3, wherein the user voice print is provided by the user by providing a spoken user name to a voice recognition unit.
6. The method of claim 3, wherein the user payment information includes a credit card number and an associated credit card expiration date.
7. The method of claim 1, wherein the step of receiving a user-spoken transaction identifier and the step of receiving a user-spoken verification identifier are performed within a predetermined time from completing the electronic transaction.
8. The method of claim 7, wherein the predetermined time is about five minutes.
9. The method of claim 1, wherein the electronic data link includes the Internet.
10. The method of claim 1, wherein the electronic data link includes a private network.
11. The method of claim 1, wherein the computer is a system component of a financial institution.
12. The method of claim 11, wherein the financial institution is a bank.
13. The method of claim 12, wherein the user conducts the electronic transaction using an ATM machine.
14. The method of claim 12, wherein the user conducts the electronic transaction by communicating with a bank teller.
15. The method of claim 1, wherein the user conducts the electronic transaction using a personal computer.
16. The method of claim 1, wherein the user conducts the electronic transaction using a wireless device.
17. The method of claim 1, wherein the user conducts the electronic transaction using a hand-held device.
18. The method of claim 1, wherein the computer is a system component of an Internet web-site.
19. The method of claim 18, further comprising:
receiving at least one user-spoken command for controlling web-site navigation, the at least one user-spoken command being transmitted by the user via a telephonic voice connection;
converting the at least one user-spoken command into at least one computer readable command;
transmitting the at least one computer readable command to the computer; and
executing the at least one computer readable command, using the computer, whereby the user controls web-site navigation of the Internet web-site by voice command via the telephonic voice connection.
20. The method of claim 19, wherein the user is prompted by a voice menu system to respond to voice menu options when transmitting the at least one user-spoken command.
21. The method of claim 1, further comprising:
providing at least one voice menu option to the user;
processing at least one user-spoken response to the at least one voice menu option, whereby the at least one user-spoken response is transformed into at least one computer-readable response;
transmitting the at least one computer-readable response to the computer; and
executing the at least one computer response, using the computer, whereby the user controls the computer by voice command.
22. The method of claim 1, wherein the user-spoken transaction identifier and the user-spoken verification identifier are transmitted by a telephonic voice connection.
23. The method of claim 1, wherein the electronic transaction includes an on-line purchase of goods or services.
24. The method of claim 1, wherein the electronic transaction includes a banking transaction.
25. The method of claim 17 wherein the electronic transaction includes downloading music files.
26. The method of claim 1, wherein the electronic transaction includes a point-of-sale transaction.
27. A system for authenticating an electronic transaction between a first user-operated device and a computer, the computer being configured to conduct electronic transactions, the system comprising:
a voice browser configured to receive and process user-spoken information from a second user-operated device, the voice browser being programmed to compare a user-spoken transaction identifier to a computer generated transaction identifier, and to compare a user-spoken verification identifier to a voice print of the user; and
a session correlator coupled to the voice browser, the session correlator being configured to transmit an authentication message to the computer if the user-spoken transaction identifier matches the computer transaction identifier, and if the user-spoken verification identifier matches the voice print.
28. The system of claim 27, wherein the voice browser further comprises:
a voice recognition unit coupled to the second user-operated device via a network, the voice recognition unit being configured to recognize audible tones transmitted over the network; and
a database coupled to the voice recognition unit, the database being configured to store the voice print of the user and payment information associated with the voice print.
29. The system of claim 28, wherein the voice recognition unit recognizes both spoken input and DTMF input.
30. The system of claim 28, further comprising a telephony interface unit coupled to the voice recognition unit, the telephony interface unit being configured to convert signals carried by the network into signals having a correct format and amplitude.
31. The system of claim 27, wherein the voice browser further comprises a voice menu system, the voice menu system comprising:
a voice menu option library having stored therein at least one voice menu option;
a user interface transmitter configured to transmit the at least one voice menu option to the user, the user interface transmitter including a synthesized speech unit for generating the at least one voice menu option, and a digitized audio unit for generating user-audible signaling tones; and
a user interface receiver configured to recognize a plurality of user-spoken menu selections provided by the user in response to the at least one voice menu option.
32. The system of claim 27, wherein the voice browser includes a digital signal processor.
33. The system of claim 27, wherein the voice browser includes at least one software module resident in an Internet backbone.
34. The system of claim 27, wherein the voice browser includes at least one software module resident in a telecommunications switch.
35. The system of claim 27, wherein the voice browser includes at least one software module resident in a computer disposed in a network data center.
36. The system of claim 27, wherein the voice browser includes at least one software module resident in a computer disposed in a customer premise equipment.
37. The system of claim 27, wherein the voice browser includes at least one software module resident in a computer disposed in an intranet.
38. The system of claim 27, wherein the session correlator includes at least one software module resident in an Internet backbone.
39. The system of claim 27, wherein the session correlator includes at least one software module resident in a telecommunications switch.
40. The system of claim 27, wherein the session correlator includes at least one software module resident in a computer disposed in a network data center.
41. The system of claim 27, wherein the session correlator includes at least one software module resident in a computer disposed in a customer premise equipment.
42. The system of claim 27, wherein the session correlator includes at least one software module resident in a computer disposed in an intranet.
43. The system of claim 27, wherein the second user-operated device includes a microphone.
44. The system of claim 27, wherein the second user-operated device includes a telephone set.
45. The system of claim 44, wherein the telephone set is a wireless telephone.
46. The system of claim 45, wherein the wireless telephone is configured to use a wireless access protocol.
47. The system of claim 27, wherein the computer transaction identifier is generated by the computer in response to the electronic transaction conducted between the user and the computer.
48. The system of claim 27, wherein the electronic data link includes the Internet.
49. The system of claim 27, wherein the electronic data link includes a private network.
50. The system of claim 27, wherein the computer is a system component of a financial institution.
51. The system of claim 50, wherein the financial institution is a bank.
52. The system of claim 51, wherein the first user-operated device includes an ATM machine.
53. The system of claim 51, wherein the user conducts the electronic transaction by communicating with a bank teller.
54. The system of claim 27, wherein the first user-operated device includes a personal computer.
55. The system of claim 27, wherein the first user-operated device includes a wireless device.
56. The system of claim 27, wherein the first user-operated device includes a hand-held device.
57. The system of claim 27, wherein the computer is a system component of an Internet web-site.
58. The system of claim 27, further comprising:
a user authentication input unit coupled to the first user-operated device and the session correlator, the user authentication unit being configured to accept a user name and a user password from the user;
a database coupled to the user authentication input unit, the database being configured to store an authentic user name and an authentic user password; and
a user authenticator coupled to the user authentication input unit, the database, and the session correlator, the user authenticator being programmed to compare the user name to the authentic user name, and to compare the user password to the authentic user password, whereby the user authenticator provides the session correlator with a transaction denial message if the user name does not match the authentic user name, or the user password does not match the authentic user password.
59. The system of claim 27, wherein the electronic transaction includes an on-line purchase of goods or services.
60. The system of claim 27, wherein the electronic transaction includes a banking transaction.
61. The system of claim 27, wherein the electronic transaction includes downloading music files.
62. The system of claim 27, wherein the electronic transaction includes a point-of-sale transaction.
63. A computerized voice verification method for authenticating an electronic transaction between a user and a computer, the computer being configured to conduct electronic transactions, the method comprising:
enrolling the user in a voice verification system, whereby the user provides the system with a user voice print;
performing the electronic transaction;
receiving a transaction identifier from the computer via an electronic data link in response to performing the electronic transaction;
receiving a user-spoken transaction identifier and a user-spoken verification identifier transmitted by the user via a voice connection;
comparing the user-spoken transaction identifier with the computer transaction identifier and the user-spoken verification identifier with a voice print of the user; and
transmitting an authentication message to the computer if the user-spoken transaction identifier matches the computer transaction identifier, and if the user-spoken verification identifier matches the voice print.
64. The method of claim 63, wherein a transaction denied message is transmitted to the computer if the user-spoken transaction identifier does not match the computer transaction identifier, or if the user-spoken verification identifier does not match the voice print.
65. A computerized method for controlling web-site navigation, the method comprising:
providing an authentication system including a voice recognition unit and a session correlator, the voice recognition unit having access to a pre-registered voice print of the user, whereby the authentication system is coupled to a user computer and a web-site during the computerized method;
conducting a transaction between the user computer and the web-site, the web-site transmitting a transaction identifier to the user computer and the authentication system in response to the transaction;
receiving a user-spoken transaction identifier and a user-spoken verification identifier via a telephonic connection, the authentication system being programmed to compare the user-spoken transaction identifier to the transaction identifier and the user-spoken verification identifier to the pre-registered voice print;
transmitting an authentication message to the web-site if the user-spoken transaction identifier matches the transaction identifier and if the user-spoken verification identifier matches the voice print;
receiving at least one user-spoken command for controlling web-site navigation, the authentication system being programmed to convert the at least one user-spoken command into at least one computer-readable command; and
transmitting the at least one computer-readable command to the web-site, the at least one computer-readable command being executed by the web-site, whereby the user controls web-site navigation of the web-site by the at least one user-spoken command.
66. The method of claim 65, wherein the at least one user-spoken command includes a plurality of user-spoken commands.
67. The method of claim 65, wherein the plurality of user-spoken commands are transmitted by the user in response to a plurality of voice menu options provided by the authentication unit.
68. The method of claim 65, wherein a web navigation is denied message is transmitted to the computer if the user-spoken transaction identifier does not match the computer transaction identifier, or if the user-spoken verification identifier does not match the voice print.