1. A method of preparing a cow brain homogenate, comprising the steps of:
reacting a composition comprising a cow brain homogenate in the presence of a metalloendopeptidase where in the homogenate composition comprises PrP protein with a PrPC conformation and a PrPSc conformation;
continuing the reacting for a period of time and under conditions sufficient to hydrolyze the PrPC conformation of the protein and not hydrolyzing the PrPSc conformation of the protein; and
changing the conditions in a manner so as to inhibit the metalloendopeptidase thereby providing a treated cow brain homogenate composition.
2. The method of claim 1, wherein the conditions are changed by the addition of an enzyme inhibitor.
3. The method of claim 2, wherein the enzyme inhibitor is ethylene diaminetetraacetic acid (EDTA).
4. A method of preparing a cow brain homogenate, comprising the steps of:
reacting a composition comprising cow brain homogenate in the presence of a metalloendopeptidase wherein the homogenate composition is suspected of comprising PrP protein in a PrPC conformation and a PrPSc conformation;
continuing the reacting for a period of time and under conditions sufficient to hydrolyze substantially all PrPC in the composition and not hydrolyzing PrPSc in the composition; and
changing the conditions in a manner so as to inhibit the metalloendopeptidase thereby providing a treated cow brain homogenate composition.
5. The method of claim 4, wherein the metalloendopeptidase is inhibited by the addition of ethylene diaminetetraacetic acid (EDTA).
6. The method of claim 4, further comprising:
contacting the treated cow brain homogenate composition with an enzyme selected from the group consisting of Dispase and Proteinase K; and
adding an enzyme inhibitor after an epitope on PrPSc in the treated cow brain homogenate composition not exposed prior to reacting in the presence of the metalloendopeptidase has been exposed, thereby providing treated PrPSc.
7. The method of claim 6, further comprising:
contacting the treated cow brain composition with a labeled antibody which binds treated PrPSc.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
What is claimed is:
1. A modular connector for attaching an interior equipment of a vehicle onto an interior wall member covering an inner face of a vehicle body panel and for supplying electric power to the interior equipment, comprising:
a connector member attached onto the interior wall member, and provided with a through hole and a first electric wire for supplying the electric-power to the interior equipment;
a bracket member provided with the interior equipment and a tube-shaped body through which a second electric wire is led out from the interior equipment, the tube-shaped body being inserted into the through hole to engaged with the connector member to electrically connect the first and second electric wires;
a wire holder protruded from an outer peripheral face of the tube-shaped body and extending in an axial direction of the tube-shaped body, for holding the second electric wire;
a first engagement member arranged in a portion defined as a spatial region surrounded by the wire holder, the outer peripheral face of the tube-shaped body, and a tangent line connecting a ridge portion of the wire holder and the outer peripheral face of the tube-shaped body; and
a second engagement member provided in a circumferential portion of the through hole of the connector member, for being engaged with the first engaging member to retain the bracket member onto the connector member.
2. The modular connector as set forth in claim 1, wherein a slit from which the second electric wire is inserted is formed in the ridge portion of the wire holder.