What is claimed is:
1. An isolated nucleic acid molecule comprising a polynucleotide selected from the group consisting of:
(a) the polynucleotide shown as SEQ ID NO:X or the polynucleotide encoded by a cDNA included in ATCC Deposit No:Z;
(b) a polynucleotide encoding a biologically active polypeptide fragment of SEQ ID NO:Y or a biologically active polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:Z;
(c) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:Y or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:Z;
(d) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(c), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.
2. The isolated nucleic acid molecule of claim 1, wherein the polynucleotide comprises a nucleotide sequence encoding a soluble polypeptide.
3. The isolated nucleic acid molecule of claim 1, wherein the polynucleotide comprises a nucleotide sequence encoding the sequence identified as SEQ ID NO:Y or the polypeptide encoded by the cDNA sequence included in ATCC Deposit No:Z.
4. The isolated nucleic acid molecule of claim 1, wherein the polynucleotide comprises the entire nucleotide sequence of SEQ ID NO:X or a cDNA included in ATCC Deposit No:Z.
5. The isolated nucleic acid molecule of claim 2, wherein the polynucleotide is DNA.
6. The isolated nucleic acid molecule of claim 3, wherein the polynucleotide is RNA.
7. A vector comprising the isolated nucleic acid molecule of claim 1.
8. A host cell comprising the vector of claim 7.
9. A recombinant host cell comprising the nucleic acid molecule of claim 1 operably limited to a heterologous regulating element which controls gene expression.
10. A method of producing a polypeptide comprising expressing the encoded polypeptide from the host cell of claim 9 and recovering said polypeptide.
11. An isolated polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
(a) the polypeptide shown as SEQ ID NO:Y or the polypeptide encoded by the cDNA;
(b) a polypeptide fragment of SEQ ID NO:Y or the polypeptide encoded by the cDNA;
(c) a polypeptide epitope of SEQ ID NO:Y or the polypeptide encoded by the cDNA; and
(d) a variant of SEQ ID NO:Y.
12. The isolated polypeptide of claim 11, comprising a polypeptide having SEQ ID NO:Y.
13. An isolated antibody that binds specifically to the isolated polypeptide of claim 11.
14. A recombinant host cell that expresses the isolated polypeptide of claim 11.
15. A method of making an isolated polypeptide comprising:
(a) culturing the recombinant host cell of claim 14 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.
16. The polypeptide produced by claim 15.
17. A method for preventing, treating, or ameliorating a medical condition, comprising administering to a mammalian subject a therapeutically effective amount of the polynucleotide of claim 1.
18. A method of diagnosing a pathological condition or a susceptibility to a pathological condition in a subject comprising:
(a) determining the presence or absence of a mutation in the polynucleotide of claim 1; and
(b) diagnosing a pathological condition or a susceptibility to a pathological condition based on the presence or absence of said mutation.
19. A method of diagnosing a pathological condition or a susceptibility to a pathological condition in a subject comprising:
(a) determining the presence or amount of expression of the polypeptide of claim 11 in a biological sample; and
(b) diagnosing a pathological condition or a susceptibility to a pathological condition based on the presence or amount of expression of the polypeptide.
20. A method for identifying a binding partner to the polypeptide of claim 11 comprising:
(a) contacting the polypeptide of claim 11 with a binding partner; and
(b) determining whether the binding partner effects an activity of the polypeptide.
21. A method of screening for molecules which modify activities of the polypeptide of claim 11 comprising:
(a) contacting said polypeptide with a compound suspected of having agonist or antagonist activity; and
(b) assaying for activity of said polypeptide.
22. A method for preventing, treating, or ameliorating a medical condition, comprising administering to a mammalian subject a therapeutically effective amount the polypeptide of claim 11.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A system for producing a renewable gasoline from cellulosic biomass comprising:
a) A cellulosic feedstream;
b) An ionic separator (ISEP) generating a sugarpolyol stream (i) and a watercarboxylate stream (ii):
i) A polyol stream system comprising:
(1) A hydrotreating (HT) reactor to generate hydrocarbons from sugars and polyols,
(2) An isomerization (ISOM) reactor to generate isomerized gasoline range hydrocarbons;
ii) A carboxylate stream system comprising:
(1) A hydrodeoxygenation (HDO) reactor generating gasoline from the carboxylate stream, hydrogen, and a portion of the hydrocarbon stream (i),
(2) a cold separator to separate alcohols, water, and hydrogen, and
(3) a membrane separator to remove water from alcohol; and
c) a mixer for one or more product streams comprising alcohol and gasoline range hydrocarbon products.
2. The renewable gasoline of claim 1, wherein said gasoline is premium, E10, E20, E85, or other blended gasoline product.
3. The renewable gasoline of claim 1, wherein said HDO reactor comprises a catalyst selected from the group consisting of CuO, ZnO, Fe2O3, CuOZnO, CuOFe2O3, ZnOFe2O3, CuOZnOFe2O3, CuOZnOAl2O3, CuOAl2O3SiO2, CuOFe2O3Al2O3SiO2, and combinations thereof.
4. The renewable gasoline of claim 1, wherein said pervaporation membrane comprises a membrane selected from the group consisting of hydrophilic, hydrophobic, organophillic, polydimethylsiloxane (PDMS), polyvinyl alcohol (PVA), polyimide, ceramic, zeolite, amorphous silica, hybrid membranes, and combinations thereof.
5. A method for producing a renewable gasoline from cellulosic biomass comprising:
a) feeding a cellulosic feedstream;
b) using an ionic separator (ISEP) to generate a sugarpolyol stream (i) and a watercarboxylate stream(ii):
i) generating hydrocarbons from sugars and polyols using a hydrotreating (HT) reactor and converting the hydrocarbons to isomerized gasoline range hydrocarbons using an isomerization (ISOM) reactor.
ii) generating alcohol from the carboxylate stream (b), hydrocarbons (i) and recycled hydrogen in a hydrodeoxygenation (HDO) reactor, separating alcohol, water, and hydrogen using a cold separator, and removing water using a membrane separator; and
c) producing one or more product streams comprising alcohol and gasoline range hydrocarbons.
6. The method of claim 5, wherein said renewable gasoline is premium, E10, E20, E85, or other blended gasoline product.
7. The method of claim 5, wherein said HDO reactor comprises a catalyst selected from the group consisting of CuO, ZnO, Fe2O3, CuOZnO, CuOFe2O3, ZnOFe2O3, CuOZnOFe2O3, CuOZnOAl2O3, CuOAl2O3SiO2, CuOFe2O3Al2O3SiO2, and combinations thereof.
8. The method of claim 5, wherein said pervaporation membrane comprises a membrane selected from the group consisting of hydrophilic, hydrophobic, organophillic, polydimethylsiloxane (PDMS), polyvinyl alcohol (PVA), polyimide, ceramic, zeolite, amorphous silica, hybrid membranes, and combinations thereof
9. A renewable gasoline produced from cellulosic biomass comprising:
a) feeding a cellulosic feedstream;
b) using a separator (ISEP) to generate a polyol stream(i) and a watercarboxylate stream(ii):
i) generating hydrocarbons from polyols using a hydrodesulfurization (HDS) reactor and converting the hydrocarbons to isomerized gasoline range hydrocarbons using an isomerization (ISOM) reactor.
ii) generating alcohol from the carboxylate stream (b), hydrocarbons (i) and recycled hydrogen in a hydrodeoxygenation (HDO) reactor, separating alcohol, water, and hydrogen using a cold separator, and removing water using a membrane separator; and
c) producing a renewable gasoline by blending alcohol and gasoline range hydrocarbons.
10. The method of claim 9, wherein said renewable gasoline is premium, E10, E20, E85, or other blended gasoline product.
11. The method of claim 9, wherein said HDO reactor comprises a catalyst selected from the group consisting of CuO, ZnO, Fe2O3, CuOZnO, CuOFe2O3, ZnOFe2O3, CuOZnOFe2O3, CuOZnOAl2O3, CuOAl2O3SiO2, CuOFe2O3Al2O3SiO2, and combinations thereof.
12. The method of claim 9, wherein said pervaporation membrane comprises a membrane selected from the group consisting of hydrophilic, hydrophobic, organophillic, polydimethylsiloxane (PDMS), polyvinyl alcohol (PVA), polyimide, ceramic, zeolite, amorphous silica, hybrid membranes, and combinations thereof.