1. A mild low temperature transcription control element consisting of a nucleotide sequence:
x1ccccX6x7X8
wherein x1 represents t, g, or a; x6 represents g, a, or c; x7 represents c, a or t; x8 represents c, a or t, or a nucleotide sequence complementary to the nucleotide sequence.
2. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is tcccc gcc (SEQ ID NO:2).
3. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is gcccc gcc (SEQ ID NO:9).
4. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is acccc gcc (SEQ ID NO:10).
5. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is tcccc gtc (SEQ ID NO:11).
6. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is tcccc gct (SEQ ID NO:12).
7. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is tcccc gaa (SEQ ID NO:21).
8. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is ggcgg gga (SEQ ID NO:22).
9. The mild low temperature transcription control element of claim 1, wherein the nucleotide sequence is ggggg gga (SEQ ID NO:7).
10. A mild low temperature transcription control enhancer wherein identical or different mild low temperature transcription control elements of claim 1 are connected.
11. A mild low temperature transcription control enhancer wherein identical or different mild low temperature transcription control elements of claim 1 are connected via other nucleotide(s).
12. A vector comprising the mild low temperature transcription control element of any one of claims 1-9 or the mild low temperature transcription control enhancer of claim 10 or 11.
13. A mammalian cell transformed with the vector of claim 12.
14. A method for screening a mild low temperature transcription control element comprising:
1) providing a gene capable of accelerating transcription at a mild low temperature, particularly at a cultivation temperature of 32\xb0 C.;
2) cloning the range of from 1.5 kilo bases located on 5\u2032 upstream of transcription initiation site of the gene to a first exon of the gene;
3) incorporating the cloned nucleotide sequence with an appropriate promoter into a vector expressing chloramphenicol actyltransferase (CAT) as a reporter gene;
4) transforming mammalian cells with the said vector;
5) cultivating the cells at a mild low temperature and 37\xb0 C., respectively, to express reporter gene to obtain a nucleotide sequence capable of accelerating the transcription of the reporter gene under mild low temperature; and
6) shortening the nucleotide sequence to be incorporated into the vector to identify the nucleotide sequence necessary for accelerating the transcription of the reporter gene at mild low temperature.
15. A method for improving a mild low temperature transcription control element comprising:
1) synthesizing a mutated nucleotide sequence in which one or two nucleotides in the mild low temperature transcription control element are substituted with other nucleotide;
2) incorporating the mutated nucleotide sequence with an appropriate promoter into a vector expressing chloramphenicol actyltransferase (CAT) as a reporter gene;
3) transforming mammalian cells with the said vector;
4) cultivating the cells at a mild low temperature and 37\xb0 C., respectively, to express the reporter gene to identify the nucleotide sequence necessary for accelerating the transcription of the reporter gene at a mild low temperature.
16. A mild low temperature translation control element comprising the nucleotide sequence: actcgcgcct taggaagctt gggtgtgtgt ggcgcgctgt cttcccgctc gcgtcaggga cctgcccgac tcagcggctg cc (SEQ ID NO:23) located 5\u2032 to code sequence and 3\u2032 to promoter.
17. A vector comprising the mild low temperature translation control element of claim 16.
18. A mammalian cell transformed with the vector of claim 17.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An apparatus comprising:
a rotational locking device, the rotational locking device including:
a first portion having a substantially planar outer surface and an inner surface substantially parallel to the outer surface,
a second portion having a substantially planar outer surface and an inner surface substantially parallel to the outer surface, the first portion is rotationally coupled to the second portion and the inner surface of the first portion interfaces with the inner surface of the second portion,
means for locking the first portion in relation to the second portion, the means for locking including a locking device having an engaging portion and a top portion, the engaging portion comprising a substantially L shaped configuration laterally offset from the top portion such that the engaging portion is positioned between the inner surface of the first portion and the inner surface of the second portion and the top portion extends beyond the inner surfaces of the first portion and the second portion; and
a holster coupled to the rotational locking device;
wherein the rotational locking device locks the holster in a plurality of positions and the engaging portion prevents the first portion from rotating when disposed within one of a plurality of locking grooves formed on the first portion.
2. The apparatus of claim 1, wherein the locking device further comprises a bottom portion and a spring coupled to the bottom portion.
3. The apparatus of claim 1, wherein the plurality of locking grooves are separated from one another a predetermined distance, and the plurality of locking grooves are arranged at predetermined angles in relation to a center of the first portion.
4. The apparatus of claim 1, the second portion comprising:
a first groove and a second groove, the first groove slidably coupled to the means for locking.
5. The apparatus of claim 1, further comprising:
a belt coupling portion, wherein the belt coupling portion is coupled to the rotational locking device.
6. The apparatus of claim 1, wherein when the engaging member is disposed within one of the locking grooves formed on the first portion, the first portion cannot rotate unless a user applies radial force on the top portion of the locking member.
7. The apparatus of claim 1, wherein the holster is an expandable baton holster comprising a first cylindrical portion and a second cylindrical portion, wherein the second cylindrical portion is tapered.
8. The apparatus of claim 1, wherein each of the plurality of grooves comprise an open end and a closed end opposite the open end.
9. A holster system comprising:
a locking device, the locking device including a first portion having an inner surface and an outer surface, a second portion having an inner surface contacting the inner surface of the first portion and an outer surface, and a locking member, the locking member comprising an engaging portion positioned between the inner surface of the first portion and the inner surface of the second portion and a top portion extending above the first portion and the second portion, the engaging portion comprising a substantially L shaped configuration laterally offset from the top portion of the locking member;
a holster coupled to the locking device;
a belt coupling device coupled to the locking device;
wherein the locking device locks the holster in a plurality of angled positions in relation to the belt coupling device, and the engaging portion completely prevents the first portion from rotating when the engaging portion is disposed within one of a plurality of grooves extending from a center portion of the first portion.
10. A holster system comprising:
a locking device, the locking device including a first portion, a second portion, and a locking member, the locking member having an engaging portion comprising a substantially L shaped configuration laterally offset from a top portion of the locking member, the locking member movable in a radial direction relative to the first portion upon the application of a force by a user in the radial direction;
a holster coupled to the locking device;
a belt coupling device coupled to the locking device;
wherein the locking device locks the holster in a plurality of angled positions in relation to the belt coupling device; the locking member having an engaging portion movable in the radial direction; the engaging portion prevents the first portion from rotating when the engaging portion is inserted in the radial direction within one of a plurality of grooves extending from a center portion of the first portion.
11. The holster system of claim 10, wherein the first portion is rotationally coupled to the second portion.
12. The holster system of claim 10, wherein the plurality of grooves are separated from one another a predetermined distance, and the plurality of grooves are arranged at predetermined angles in relation to the center of the first portion.
13. The holster system of claim 10, wherein the holster is an expandable baton holster.
14. The holster system of claim 13, the expandable baton holster comprising a first cylindrical portion and a second cylindrical portion, wherein the second cylindrical portion is tapered.
15. The holster system of claim 10, wherein the locking member has a top portion, which is accessible from above the first portion and the second portion.