1. A photosensitive member cartridge, comprising:
a photosensitive drum;
a cartridge frame configured to connect to a developer cartridge; and
a lock device comprising:
a rotating shaft;
a lock lever rotatable about the rotating shaft, the lock lever including a contact portion; and
a resin-made spring integrally formed as one piece with the contact portion and extending from the contact portion, a portion of the spring contacting the cartridge frame;
wherein the contact portion is configured to be positioned relative to an action receiving member of the developer cartridge when the developer cartridge is connected to the cartridge frame, thereby establishing a locked state preventing the developer cartridge from becoming disconnected from the cartridge frame.
2. The photosensitive member cartridge of claim 1, wherein the rotating shaft extends through the cartridge frame.
3. The photosensitive member cartridge of claim 1, wherein the spring extends downward from a bottom portion of the lock lever.
4. The photosensitive member cartridge of claim 1, wherein:
the cartridge frame comprises a restriction piece extending from the cartridge frame; and
the spring contacts the restriction piece.
5. The photosensitive member cartridge of claim 1, further comprising:
an urging device configured to cause a developer roller of the developer cartridge to be pressed against the photosensitive drum.
6. The photosensitive member cartridge of claim 5, wherein the urging device is configured to contact the action receiving member to cause the developer roller to be pressed against the photosensitive drum.
7. The photosensitive member cartridge of claim 1, wherein the lock lever is positioned such that:
during insertion of the developer cartridge into to the cartridge frame, the action receiving member contacts the lock lever, thereby causing the lock lever to be rotated from a first position to a second position against a force of the spring; and
when the developer cartridge is connected to the cartridge frame, the force of the spring causes the lock lever to return to the first position where the action receiving member is facing a lower surface of the contact portion, thereby establishing the locked state that prevents the developer cartridge form becoming disconnected from the cartridge frame.
8. The photosensitive member cartridge of claim 1, wherein the contact portion of the lock lever is configured to contact the action receiving member of the developer cartridge when the developer cartridge is connected to the cartridge frame, thereby establishing the locked state preventing the developer cartridge from becoming disconnected from the cartridge frame.
9. A process unit, comprising:
the photosensitive member cartridge of claim 1; and
the developer cartridge connected to the cartridge frame.
10. The process unit of claim 9, wherein the contact portion of the lock lever is positioned relative to the action receiving member of the developer cartridge such that the developer cartridge cannot be disconnected from the cartridge frame.
11. The process unit of claim 10, wherein a force of the spring positions the lock lever relative to the action receiving member.
12. The process unit of claim 10, wherein the contact portion of the lock lever is facing the action receiving member of the developer cartridge such that the developer cartridge cannot be disconnected from the cartridge frame.
13. The process unit of claim 12, wherein the contact portion of the lock lever contacts the action receiving member of the developer cartridge such that the developer cartridge cannot be disconnected from the cartridge frame.
14. The process unit of claim 9, wherein:
the cartridge frame comprises a restriction piece extending from the cartridge frame; and
the spring contacts the restriction piece.
15. The process unit of claim 9, wherein the rotating shaft extends through the cartridge frame.
16. The process unit of claim 9, wherein the spring extends downward from a bottom portion of the lock lever.
17. The process unit of claim 9, wherein:
the photosensitive member cartridge further comprises an urging device that causes a developer roller of the developer cartridge to be pressed against the photosensitive drum.
18. The process unit of claim 17, wherein the urging device contacts the action receiving member to cause the developer roller to be pressed against the photosensitive drum.
19. A photosensitive member cartridge, comprising:
a photosensitive drum;
a cartridge frame configured to connect to a developer cartridge;
a restriction piece extending from the cartridge frame; and
a lock device comprising:
a rotating shaft;
a lock lever rotatable about the rotating shaft, the lock lever including a contact portion; and
a spring extending from the contact portion, a portion of the spring contacting the restriction piece;
wherein the contact portion is configured to be positioned relative to an action receiving member of the developer cartridge when the developer cartridge is connected to the cartridge frame, thereby establishing a locked state preventing the developer cartridge from becoming disconnected from the cartridge frame.
20. A photosensitive member cartridge, comprising:
a photosensitive drum;
a cartridge frame configured to connect to a developer cartridge; and
a lock device comprising:
a rotating shaft;
a lock lever rotatable about the rotating shaft, the lock lever including a contact portion; and
a spring extending from the contact portion, a portion of the spring contacting the cartridge frame;
wherein:
the contact portion is configured to be positioned relative to an action receiving member of the developer cartridge when the developer cartridge is connected to the cartridge frame, thereby establishing a locked state preventing the developer cartridge from becoming disconnected from the cartridge frame; and
the lock lever is positioned such that:
during insertion of the developer cartridge into to the cartridge frame, the action receiving member contacts the lock lever, thereby causing the lock lever to be rotated from a first position to a second position against a force of the spring; and
when the developer cartridge is connected to the cartridge frame, the force of the spring causes the lock lever to return to the first position where the action receiving member is facing a lower surface of the contact portion, thereby establishing the locked state that prevents the developer cartridge form becoming disconnected from the cartridge frame.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A desalting tip column comprising:
i) a column body having an open upper end, an open lower end, and an open channel between the upper and lower ends of the column body;
ii) a bottom membrane screen bonded to and extending across the open channel;
iii) a top frit bonded to and extending across the open channel between the bottom membrane screen and the open upper end of the column body, wherein the top frit, bottom membrane screen, and column body define a media chamber; and
iv) a packed bed of size exclusion media positioned inside the extraction media chamber.
2. The desalting tip column of claim 1 wherein,
the packed bed of size exclusion media comprises an interstitial space that is substantially full of glycerol.
3. The desalting tip column of claim 1 wherein,
the open upper end of the desalting tip column body is attached to a pipettor.
4. A plurality of desalting tip columns comprised of:
i) a column body having an open upper end, an open lower end, and an open channel between the upper and lower ends of the column body;
ii) a bottom frit bonded to and extending across the open channel;
iii) a top frit bonded to and extending across the open channel between the bottom frit and the open upper end of the column body, wherein the top frit, bottom frit, and column body define a media chamber; and
iv) a packed bed of size exclusion media positioned inside the extraction media chamber.
5. The plurality of desalting tip columns of claim 4, wherein
the packed bed of size exclusion media comprises an interstitial space that is substantially full of glycerol.
6. The plurality of desalting tip columns of claim 4, further comprising a protein sample.
7. The plurality of desalting tip columns of claim 6, wherein the protein sample was eluted from a pipette-tip column.
8. The plurality of desalting tip columns of claim 4, wherein the open upper ends of the desalting columns are attached to a pump.
9. The plurality of desalting tip columns of claim 8, wherein the pump is a syringe pump.
10. The plurality of desalting tip columns of claim 9, wherein the syringe pump is a multichannel pipettor.
11. The plurality of desalting tip columns of claim 8, further comprising:
a controller, wherein said controller controls the pump; and
a computer, wherein said computer can be programmed to control the movement of the pump through the controller.
12. A method for desalting a protein sample comprising:
(a) depositing the protein sample onto a desalting tip column, wherein said desalting tip column is comprised of
i) a column body having an open upper end, an open lower end, and an open channel between the upper and lower ends of the column body;
ii) a bottom frit bonded to and extending across the open channel;
iii) a top frit bonded to and extending across the open channel between the bottom frit and the open upper end of the column body, wherein the top frit, bottom frit, and column body define a media chamber; and
iv) a packed bed of size exclusion media positioned inside the extraction media chamber.
(b) attaching the open upper end of the desalting tip column to a pump;
(c) pumping the protein sample through the desalting tip column.
13. The method for desalting a protein sample of claim 12, wherein following step (c), the method is further comprised of
(d) depositing a chaser solution onto the desalting tip column; and
(e) pumping the chaser through the desalting tip column.
14. The method for desalting a protein sample of claim 12 wherein the protein sample is eluted from a pipette-tip column.
15. The method for desalting a protein sample of claim 12, wherein
the pump is a syringe pump.
16. The method for desalting a protein sample of claim 15, wherein the syringe pump is a pipettor.
17. A method for desalting a plurality of protein samples, comprising:
(a) depositing protein samples onto a plurality of desalting tip columns of claim 4;
(b) attaching the open upper ends of the desalting tip columns to a pump; and
(c) pumping the protein samples through the desalting tip columns.
18. The method for desalting a plurality of protein samples of claim 17, wherein following step (c) the method is further comprised of:
(d) depositing a chaser solution onto the desalting tip columns;
(e) pumping the chaser through the desalting tip columns.
19. The method for desalting a plurality of protein samples of claim 17, wherein
the pump is a multichannel pipettor.
20. The method for desalting a plurality of protein samples of claim 17, wherein the pump is further comprised of:
a controller; and
a computer, wherein said computer can be programmed to control the movement of the pump through the controller.
21. The method for desalting a plurality of protein samples of claim 17, wherein the protein samples are eluted from pipette tip columns.