1. A method to create a process plant operator interface, the method comprising:
receiving a search criterion;
identifying a user interface module based on the search criterion; and
adding the identified user interface module to the process plant operator interface.
2. A method as defined in claim 1, wherein the search criterion comprises at least one of a word, two or more words, a phrase, a logical expression of words, a logical expression of phrases, or a logical expression of words and phrases.
3. A method as defined in claim 1, wherein identifying the user interface module comprises comparing the search criterion with at least one of metadata or embedded text associated with respective ones of two or more user interface modules.
4. A method as defined in claim 3, wherein the at least one of metadata or embedded text associated with each of the two or more user interface modules comprises at least one of an interface module title, an interface module description, or an interface module keyword.
5. A method as defined in claim 1, wherein identifying the user interface module comprises:
identifying one or more user interface modules associated with the search criterion;
presenting a list of the one or more user interface modules; and
receiving a selection of the identified user interface module.
6. A method as defined in claim 1, wherein adding the identified user interface module to the process plant operator interface comprises:
loading the user interface module into a machine accessible memory;
adding a graphic associated with the user interface module to the process plant operator interface; and
initiating execution of the user interface module.
7. A method as defined in claim 1, wherein the user interface module and the process plant operator interface are executed at a same computer.
8. A method as defined in claim 1, wherein the user interface module and the process plant operator interface are executed at different computers, and wherein the process plant operator interface displays data provided by the user interface module to the process plant operator interface.
9. A method as defined in claim 1, wherein the user interface module comprises at least one of a display of a data value, a display of a graph, a display of a graphic image, a display of current data, a display of historical data, a display of a list of data, a display of data extracted from a database, a display of data collected from a process plant controller, a display of data collected from a process plant field device, or a transaction-based interface.
10. A method as defined in claim 1, wherein identified the user interface module based on the search criterion comprises searching an interface module library.
11. A method as defined in claim 10, wherein the interface module library is stored at a computer presenting the process plant operator interface.
12. A method as defined in claim 10, wherein the control module library is stored remotely from a computer presenting the process plant operator interface.
13. An operator station apparatus comprising:
a display;
an operator display module to present a process plant operator interface on the display; and
a search engine to identify a user interface module based upon a search criterion, the operator display module to display an output of the user interface module.
14. An apparatus as defined in claim 13, further comprising a user interface module library, wherein the search criterion is compared with at least one of metadata or embedded text associated with each of a plurality interface modules stored in the user interface module library to identify the user interface module having the output to be displayed.
15. An apparatus as defined in claim 14, further comprising a network interface, wherein the user interface module library is located remotely from the operator station apparatus, and the user interface module library is searched via the network interface.
16. An apparatus as defined in claim 14, wherein the at least one of metadata or embedded text associated with each of the plurality of interface modules comprises at least one of an interface module title, an interface module description, or an interface module keyword.
17. An apparatus as defined in claim 13, wherein the operator display module is to:
present a first interface to receive the search criterion;
present a second interface to present a search result and to receive an interface selection input associated with the identified user interface module; and
add the user interface module to the process plant operator interface.
18. An apparatus as defined in claim 13, wherein the user interface module comprises at least one of a display of a data value, a display of a graph, a display of a graphic image, a display of current data, a display of historical data, a display of a list of data, a display of data extracted from a database, a display of data collected from a process plant controller, a display of data collected from a process plant field device, or a transaction-based interface.
19. An apparatus as defined in claim 13, wherein the operator station apparatus comprises an operator station of a process plant control system.
20. An article of manufacture storing machine readable instructions which, when executed, cause a machine to create a process plant operator interface by:
receiving a search criterion;
identifying a user interface module based on the search criterion; and
adding the identified user interface module to the process plant operator interface.
21. An article of manufacture as defined in claim 20, wherein the search criterion comprises at least one of a word, two or more words, a phrase, a logical expression of words, a logical expression of phrases, or a logical expression of words and phrases.
22. An article of manufacture as defined in claim 20, wherein the machine readable instructions, when executed, cause the machine to identify the user interface module by comparing the search criterion with at least one of metadata or embedded text associated with respective ones of two or more user interface modules.
23. An article of manufacture as defined in claim 22, wherein the at least one of the metadata or the embedded text associated with each of the two or more user interface modules comprises at least one of an interface module title, an interface module description, or an interface module keyword.
24. An article of manufacture as defined in claim 20, wherein the machine readable instructions, when executed, cause the machine to identify the user interface module by:
identifying one or more user interface modules associated with the search criterion;
presenting a list of the one or more user interface modules; and
receiving a selection of the identified user interface module.
25. An article of manufacture as defined in claim 20, wherein the machine readable instructions, when executed, cause the machine to add the identified user interface module to the process plant operator interface by:
loading the user interface module into a machine accessible memory;
adding a graphic associated with the user interface module to the process plant operator interface; and
initiating execution of the user interface module.
26. An article of manufacture as defined in claim 20, wherein the user interface module comprises at least one of a display of a data value, a display of a graph, a display of a graphic image, a display of current data, a display of historical data, a display of a list of data, a display of data extracted from a database, a display of data collected from a process plant controller, a display of data collected from a process plant field device, or a transaction-based interface.
27. An article of manufacture as defined in claim 20, wherein the machine readable instructions, when executed, cause the machine to identify the user interface module based on the search criterion by searching an interface module library.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A Pichia pastoris cell lacking vacuolar sorting activity or having reduced vacuolar sorting activity relative to a wild-type Pichia pastoris cell, wherein the host cell comprises a functional deletion of a vacuolar protein sorting receptor 10-1 (VPS10-1).
2. The Pichia pastoris cell of claim 1; wherein the cell comprises an expression vector which comprises a sequence of nucleotides that encodes a heterologous protein.
3. The Pichia pastoris cell of claim 2, wherein the heterologous protein is a glycoprotein.
4. The Pichia pastoris cell of claim 3, wherein the cell is modified to express a glycoprotein in which the glycosylation pattern is human-like.
5. The Pichia pastoris cell of claim 1, wherein a gene encoding VPS10-1 is deleted and a gene encoding VPS10-2 is not deleted.
6. The Pichia pastoris cell of claim 1, wherein a gene encoding VPS10-1 comprises a mutation that renders the encoded Vps10-1 protein nonfunctional or incapable of vacuolar sorting activity.
7. The Pichia pastoris cell of claim 1, wherein the functional deletion of Vps10-1 activity comprises an alteration selected from the group consisting of: deletion or disruption of upstream or downstream regulatory sequences of the VPS10-1 gene, abrogation of vacuolar sorting activity by means of a chemical, peptide or protein inhibitor of Vps10-1 protein, abrogation of vacuolar sorting activity by means of a nucleic acid-based expression inhibitor and abrogation of vacuolar sorting activity by means of a transcription inhibitor.
8. A method for producing a recombinant protein in a yeast or fungal host cell comprising:
a. transforming a genetically modified yeast or fungal cell with an expression vector encoding the protein to produce a host cell, wherein the genetically modified yeast or fungal cell lacks vacuolar sorting activity or has decreased vacuolar sorting activity relative to an unmodified yeast or fungal cell of the same species;
b. culturing the transformed yeast or fungal host cell in a medium under conditions which induce expression of the protein in fermentation conditions; and
c. isolating the protein from the transformed host cell or culture medium.
9. The method of claim 8, wherein the yeast or fungal host cell is selected from the group consisting of: Pichia pastoris, Saccharomyces cerevisiae, Aspergillus niger, Schizosaccharomyces pombe, Candida albicans, Candida glabrata, Pichia stipitis, Debaryomyces hansenii, Kluyveromyces lactis, and Hansenula polymolpha.
10. The method of claim 8 or 9, wherein vacuolar sorting activity has been eliminated or reduced by deletion or disruption of a gene encoding VPS10 or a VPS10 homolog from the yeast or fungal cell genome.
11. The method of claim 10, wherein the yeast or fungal host cell is Pichia pastoris.
12. The method of claim 11, wherein the VPS10 homolog VPS10-1 is deleted.
13. A method for producing a recombinant protein in a Pichia host cell comprising:
a. transforming a genetically modified Pichia cell with an expression vector encoding the protein to produce a host cell, wherein the genetically modified Pichia cell lacks vacuolar sorting activity or has decreased vacuolar sorting activity relative to an unmodified Pichia cell of the same species;
b. culturing the transformed Pichia host cell in a medium under conditions which induce expression of the protein; and
c. isolating the protein from the transformed host cell or culture medium.
14. The method of claim 13, wherein the host cell is a Pichia pastoris host cell.
15. The method of claim 14, wherein the genetically modified Pichia pastoris cell comprises a deletion of VPS10-1.
16. The method of claim 8, wherein the genetically modified host cell comprises an alteration of the cytoplasmic domain of Vps10 or the Vps10 homolog that alters its normal trafficking pattern.
17. The method of claim 8, wherein vacuolar sorting activity is reduced or eliminated by deletion or disruption of one or more genes that are associated with the CPY vacuolar sorting pathway, wherein the one or more genes encode a protein selected from the group consisting of: Gga1, Gga2, Mvp1, Pep12, Vps1, Vps8, Vps9, Vps15, Vps21, Vps19, Vps34, Vps38, Vps45, and Vti1.
18. The method of claim 8, wherein vacuolar sorting activity is reduced or eliminated by deletion or disruption of one or more genes that encode a protein associated with recycling of Vps10 to the late Golgi, wherein the one or more genes encode a protein selected from the group consisting of: Grd19, Rgp1, Ric1, Vps5, Vps17, Vps26, Vps29, Vps30, Vps35, Vps51, Vps52, Vps53, and Vps54.
19. The method of claim 8, wherein vacuolar sorting activity is reduced or eliminated by deletion or disruption of one or more genes that encode a protein associated with MVB function, wherein the one or more genes encode a protein selected from the group consisting of: Ccz1, Fab1, Hse1, Mrl1, Vam3, Vps2, Vps3, Vps4, Vps11, Vps13, Vps16, Vps18, Vps20, Vps22, Vps23, Vps24, Vps25, Vps27, Vps28, Vps31, Vps32, Vps33, Vps36, Vps37, Vps39, Vps41, Vps43, Vps44, Vps46, Vta1, and Ypt7.
20. The method of claim 8, wherein the expression vector encodes a glycoprotein and wherein the modified host cell has been further modified to express a glycoprotein in which the glycosylation pattern is human-like.