1. A method for generating andor enriching antigen-specific T cells comprising:
(a) exposing a first population of cells wherein at least a portion thereof comprises antigen presenting cells to a paramagnetic bead wherein said paramagnetic bead has antigen attached thereto, such that said paramagnetic bead with antigen attached thereto is ingested by said APC;
(b) exposing a second population of cells wherein at least a portion thereof comprises T cells to the population of cells in part (a); thereby generating andor enriching antigen-specific T cells through direct contact between said APC and said T cells; and
(c) isolating said APCs and antigen-specific T cells by means of a magnetic field.
2. The method according to claim 1 wherein said antigen-specific T cells are expanded according to the following method:
(a) exposing said T cells to an anti-CD3 antibody which is immobilized on a surface; and
(b) stimulating an accessory molecule on the surface of the T cells with an anti-CD28 antibody, wherein said anti-CD28 antibody is immobilized on the same surface as the anti-CD3 antibody;
thereby inducing expansion of said antigen-specific T cells.
3. The method according to claim 2, further comprising exposing said T cells to IL-15.
4. The method according to claim 2, further comprising exposing said T cells to a natural ligand for CD137.
5. The method according to claim 2, further comprising exposing said T cells to an anti-CD137 antibody.
6. The method according to claim 2, further comprising exposing said T cells to an anti-NKG2D antibody or a natural ligand for NKG2D.
7. The method according to claim 2 wherein said antigen-specific T cells are expanded by exposing said antigen-specific T cells to a mitogen.
8. The method according to claim 7 wherein said mitogen is selected from the group consisting of phytohemagglutinin (PHA), phorbol myristate acetate (PMA) and ionomycin, lipopolysaccharide (LPS), and superantigen.
9. The method according to claim 1 wherein said antigen is selected from the group consisting of protein, glycoprotein, peptides, antibodyantigen complexes, whole tumor or virus-infected cells, fixed tumor or virus-infected cells, heat-killed tumor or virus-infected cells, tumor lysate, non-soluble cell debris, apoptotic bodies, necrotic cells, whole tumor cells from a tumor or a cell line that have been treated such that they are unable to continue dividing, allogeneic cells that have been treated such that they are unable to continue dividing, irradiated tumor cells, irradiated allogeneic cells, natural or synthetic complex carbohydrates, lipoproteins, lipopolysaccharides, transformed cells or cell line, transfected cells or cell line, transduced cells or cell line, and virally infected cells or cell line.
10. The method according to claim 1 wherein said antigen is attached to said surface by an antibodyligand interaction.
11. The method according to claim 10 wherein said antibodyligand interaction comprises an interaction between an antibodyligand pair selected from the group consisting of anti-MART-1 antibodyMART-1 antigen, anti-WT-1 antibodyWT-1, anti-PR1 antibodyPR1, anti-PR3 antibodyPR3, anti-tyrosinase antibodytyrosinase antigen, anti-MAGE-1 antibodyMAGE-1 antigen, anti-MUC-1 antibodyMUC-1 antigen, anti-\u03b1-fetoprotein antibody\u03b1-fetoprotein antigen, anti-Her2Neu antibodyHer2Neu, anti-HIV gp120 antibodyHIV gp120, anti-influenza HA antibodyinfluenza HA, anti-CMV pp65CMV pp65, anti-hepatitis C antibodyhepatitis C proteins, anti-EBV EBNA 3B antibodyEBV EBNA 3B antigen, and anti-human Ig heavy and light chainsIg from a myeloma cancer patient, and anti-human Ig heavy and light chainsIg from a CLL cancer patient.
12. The method according to claim 1 wherein said antigen is chemically attached to said surface.
13. The method according to claim 1 wherein the attachment of said antigen to said surface comprises a biotin-avidin interaction.
14. The method according to claim 1 wherein said population of cells wherein at least a portion thereof comprises APC is derived from a source selected from the group consisting of leukapheresis product, peripheral blood, lymph node, tonsil, thymus, tissue biopsy, tumor, spleen, bone marrow, cord blood, CD34 cells, monocytes, and adherent cells.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. An Internet refrigerator, comprising:
a server controller that transmits a display signal to a client Web pad on the basis of a remote display protocol such that data processed in said Internet refrigerator is displayed through said client Web pad;
a main controller that performs refrigeration associated functions;
an application program driver that drives a plurality of application programs installed in said Internet refrigerator; and
an Internet data processor that processes data transmitted and received over an external Internet network,
wherein said client Web pad is detachably mounted on an outer surface of said Internet refrigerator, receives a first display signal and a second display signal transmitted from said Internet refrigerator and displays the received display signals;
wherein said server controller includes an application program module that outputs a control signal to said application program driver in response to an execution command input from said client Web pad to drive one of said application programs corresponding to the input execution command, and transmits said first display signal to said client Web pad so that the corresponding application program can be displayed through said client Web pad;
wherein said server controller includes a multimedia module that transmits said second display signal to said client Web pad so that multimedia data transmitted from at least one of a television and a radio can be displayed through said client Web pad, and
wherein said server controller is configured to perform a home server function of transmitting and receiving signals to and from a plurality of home appliances connected to a home network.
2. The Internet refrigerator as set forth in claim 1, wherein said multimedia module includes a compression-encoding module that compresses said multimedia data processed by a multimedia data processor.
3. The Internet refrigerator as set forth in claim 1,
wherein said Internet refrigerator includes a terminal configured for communications with an antenna terminal of at least one of a television and a radio; and
wherein said Internet refrigerator further comprising a multimedia data processor that processes multimedia data transmitted in real time from the antenna terminal.
4. The Internet refrigerator as set forth in claim 1,
wherein said client Web pad includes a compression-decoding module that decodes compressed multimedia data transmitted from said Internet refrigerator.
5. A method for operating a client Web pad with an Internet refrigerator including a server, comprising:
detachably mounting the client Web pad on an outer surface of said Internet refrigerator and inputting a control command through the Client Web pad;
driving resources of said Internet refrigerator in response to the input control command;
transmitting, to the client Web pad, a first display signal, based on a remote display protocol, for an application program so that the application program can be displayed through said client Web pad, and transmitting, to the client Web pad, a second display signal, based on a remote display protocol, for multimedia data, so that the multimedia data can be displayed through said client Web pad, such that a driven state of the resources of the refrigerator is displayed through the client Web pad;
displaying the application program, in response to the first display signal being received by the client Web pad, and displaying the multimedia data, in response to the second display signal being received by the client Web pad, and
performing, at a server controller of the Internet refrigerator, a home server function of transmitting and receiving signals to and from a plurality of home appliances connected to a home network.
6. The method as set forth in claim 5, wherein driving the resources comprises:
connecting said Internet refrigerator with an antenna terminal of a media when the input control is a media connection request command;
receiving multimedia data transmitted from the antenna terminal; and
compression-encoding the received multimedia data.
7. The method as set forth in claim 6, wherein the displaying comprises decoding the data received by the client Web pad when the data is in a compressed state.
8. A method for operating an Internet refrigerator with a client Web pad, comprising:
detachably mounting the client Web pad on an outer surface of said Internet refrigerator and inputting a control command through the client Web pad;
driving resources of said Internet refrigerator in response to the input control command;
transmitting a first display signal for an application program and a second display signal for multimedia data to the client Web pad such that a driven state of the resources of the refrigerator is displayed through the client Web pad, at least one of the first display signal and the second display signal being based on a remote display protocol;
displaying data received by the client Web pad in response to the transmitted display signal, and
performing, at a server controller of the Internet refrigerator, a home server function of transmitting and receiving signals to and from a plurality of home appliances connected to a home network.
9. The method as set forth in claim 8, wherein driving the resources comprises:
connecting said Internet refrigerator with an antenna terminal of a media when the input control is a media connection request command;
receiving multimedia data transmitted from the antenna terminal; and
compression-encoding the received multimedia data.
10. The method as set forth in claim 9, wherein the displaying comprises decoding the data received by the client Web pad if the data is in a compressed state.