1. A method for producing a Fe\u2014Co based target material, comprising the steps of:
preparing a first raw-material powder having an Fe:Co weight ratio ranging from 8:2 to 7:3 and a second raw-material powder having an Fe\u2014Co weight ratio ranging from 2:8 to 0:10;
mixing the first raw-material powder and the second raw-material powder together to obtain a powder mixture having an Fe:Co weight ratio ranging from 8:2 to 2:8; and
applying a pressure of not less than 100 MPa to the powder mixture at a temperature ranging from 1073 to 1473 K for consolidation,
wherein at least one additional element selected from the group consisting of Nb, Zr, Ta and Hf is added to either one or both of the first and second raw-material powders in a total amount of 3 to 15 atom % with respect to the total amount of the powder mixture.
2. The method according to claim 1, wherein, prior to the mixing step, the additional element is added to the first raw-material powder in a total amount of not less than 1 atom % with respect to the total amount of the first raw-material powder.
3. The method according to claim 1 or 2, wherein, prior to the mixing step, the additional element is added to the second raw-material powder in a total amount of not less than 1 atom % with respect to the total amount of the second raw-material powder.
4. An Fe\u2014Co based target material, which is obtainable by the method according to claim 1, wherein the Fe\u2014Co based target material is made of an Fe\u2014Co based alloy comprising:
85 to 97 atom % of Fe and Co, which have a Fe:Co weight ratio ranging from 8:2 to 2:8; and
3 to 15 atom % of at least one additional element selected from the group consisting of Zr, Nb, Ta and Hf.
5. The Fe\u2014Co based target material according to claim 4, having magnetic permeability ranging from 10 to 200.
The claims below are in addition to those above.
All refrences to claim(s) which appear below refer to the numbering after this setence.
1. A method for producing a complex of labeled probes and a water-soluble carrier, comprising the following steps:
(a) reacting a probe having one or more thiol groups with an avidin compound having one or more maleimide groups to obtain a probe conjugate, wherein the probe is selected from the group consisting of protein G, protein A, protein L, lectin, and at least one antibody or antigen binding fragment thereof that is selected to bind to a target substance;
(b) introducing one or more thiol groups into the probe conjugate and reacting said probe conjugate having one or more thiol groups with a high-molecular water-soluble carrier having one or more maleimide groups to obtain a complex of the probe conjugate and the water-soluble carrier;
(c) mixing the complex of the probe conjugate and the water-soluble carrier with a biotinylated label so that the avidin compound in the complex of the probe conjugate and the water-soluble carrier reacts with biotin in the biotinylated label to form the complex of labeled probes and the water-soluble carrier; and
(d) isolating the complex of labeled probes and a water-soluble carrier.
2. The method according to claim 1, wherein the avidin compound is avidin or streptavidin.
3. The method according to claim 1, wherein the water-soluble carrier has a molecular weight of 500,000 or more.
4. The method according to claim 1, wherein the water-soluble carrier is selected from the group consisting of dextran, aminodextran, dextrin, cluster dextrin, ficoll, and pullulan.
5. The method according to claim 1, wherein the probe being capable of binding to a target substance is the at least one antibody or antigen binding fragment thereof.
6. The method according to claim 1, wherein the probe being capable of binding to a target substance is two or more kinds of antibodies or antigen binding fragments thereof.
7. The method according to claim 5, wherein the antibody or the antigen binding fragment thereof is selected from the group consisting of Fab\u2032, F(ab\u2032)2, Fab, IgG, and a combination thereof.
8. The method according to claim 1, wherein the biotinylated label is selected from the group consisting of biotinylated luciferase, biotinylated alkaline phosphatase, biotinylated peroxidase (POD), biotinylated glucose oxidase (GOD), biotinylated fluorescein isothiocyanate (FITC), biotinylated acridinium, biotinylated acridinium derivative, and biotinylated tris(2,2\u2032bipyridyl)ruthenium(II).
9. The method according to claim 8, wherein the biotinylated luciferase is produced by genetic recombination.
10. A complex of labeled probes and a water-soluble carrier produced by a method comprising the following steps:
(a) reacting a probe having one or more thiol groups with an avidin compound having one or more maleimide groups to obtain a probe conjugate, wherein the probe is selected from the group consisting of protein G, protein A, protein L, lectin, and at least one antibody or antigen binding fragment thereof that is selected to bind to a target substance;
(b) introducing one or more thiol groups into the probe conjugate and reacting said probe conjugate having one or more thiol groups with a high-molecular water-soluble carrier having one or more maleimide groups to obtain a complex of the probe conjugate and the water-soluble carrier;
(c) mixing the complex of the probe conjugate and the water-soluble carrier with a biotinylated label so that the avidin compound in the complex of the probe conjugate and the water-soluble carrier reacts with biotin in the biotinylated label to form the complex of labeled probes and the water-soluble carrier; and
(d) isolating the complex of labeled probes and a water-soluble carrier.
11. The complex of labeled probes and a water-soluble carrier of claim 10, wherein the avidin compound is avidin or streptavidin.
12. The complex of labeled probes and a water-soluble carrier of claim 10, wherein the water-soluble carrier has a molecular weight of 500,000 or more.
13. The complex of labeled probes and a water-soluble carrier of claim 10, wherein the water-soluble carrier is selected from the group consisting of dextran, aminodextran, dextrin, cluster dextrin, ficoll, and pullulan.
14. The complex of labeled probes and a water-soluble carrier of claim 10, wherein the probe being capable of binding to a target substance is the at least one antibody or antigen binding fragment thereof.
15. The complex of labeled probes and a water-soluble carrier of claim 10, wherein the probe being capable of binding to a target substance is two or more kinds of antibodies or antigen binding fragments thereof.
16. The complex of labeled probes and a water-soluble carrier of claim 14, wherein the antibody or antigen binding fragment thereof is selected from the group consisting of Fab\u2032, F(ab\u2032)2, Fab, IgG, and a combination thereof.
17. The complex of labeled probes and a water-soluble carrier of claim 10, wherein the biotinylated label is selected from the group consisting of biotinylated luciferase, biotinylated alkaline phosphatase, biotinylated peroxidase (POD), biotinylated glucose oxidase (GOD), biotinylated fluorescein isothiocyanate (FITC), biotinylated acridinium, biotinylated acridinium derivative, and biotinylated tris(2,2\u2032bipyridyl)ruthenium(II).
18. The complex of labeled probes and a water-soluble carrier of claim 17, wherein the biotinylated luciferase is produced by genetic recombination.
19. The method according to claim 6, wherein the antibodies or the antibody fragments are selected from the group consisting of Fab\u2032, F(ab\u2032)2, Fab, IgG, and a combination thereof.